Different lymphocyte markers and cytokine expression in peripheral blood mononuclear cells in children with acute atopic dermatitis.

Abstract:

BACKGROUND:The pathogenesis of atopic dermatitis (AD) is still not completely understood. AD is characterized by the presence of clinical symptoms of both IgE antibody-mediated immediate hypersensitivity and specific T lymphocyte-mediated delayed hypersensitivity. OBJECTIVE:To evaluate the immunological mechanisms involved in children with acute AD lesions. MATERIAL AND METHODS:Ten children with acute AD lesions and 10 non-atopic controls were studied. Total IgE was measured by immunoassay. T cell marker expression (CD3, CD4, CD8, cutaneous lymphocyte-associated antigen [CLA]) and cytokine production (interferon [IFN]-gamma, interleukin [IL]-13) were analyzed in peripheral blood mononuclear cells by flow cytometry. RESULTS:In children with AD the percentage of CD3+ cells (p = 0.015) increased while that of CD8+ cells (p = 0.023) decreased, with no differences in CLA expression. We found increased IL-13 production in CD3+ cells (p = 0.01) and CD3+CD4+ (p = 0.001) cells with no difference in IFN-gamma. Total IgE was significantly higher in patients with AD (p = 0.01). Comparison of IL-13 production in CD4+ cells categorized by total IgE level showed that IL-13 production was significantly increased in subjects with a higher IgE level. CONCLUSION:Peripheral blood from children with AD showed an increase in IgE levels and a Th2 pattern. There was a correlation between IL-13 production and total IgE levels.

authors

Antúnez C,Torres MJ,Corzo JL,Pena RR,Mayorga C,Jurado A,Santamaría-Babi LF,Blanca M

doi

10.1016/s0301-0546(04)79251-4

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

252-8

issue

5

eissn

0301-0546

issn

1578-1267

pii

13066301

journal_volume

32

pub_type

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