Abstract：
:Snakebites are a relevant public health problem in Central and South America. Snake bite envenomations cause intense pain, not relieved by anti-venom. The fangs of many species are short, causing subcutaneous injection. Fangs of larger species inflict subcutaneous or intramuscular envenomation. To understand pain induced by subcutaneous venom, this study examined spinal mechanisms involved in pain-enhancing effects of subcutaneous Lys49 and Asp49 secretory phospholipase-A(2) (sPLA2), two components of Bothrops asper snake venom showing highly different enzymatic activities. Unilateral intraplantar sPLA2-Lys49 (catalytically inactive) or sPLA2-Asp49 (catalytically active) into rat hindpaws each induced mechanical hyperalgesia (Randall-Selitto test), whereas only catalytically active sPLA2-Asp49 caused mechanical allodynia (von Frey test). Effects induced by both sPLA2s were inhibited by intrathecal fluorocitrate, a reversible glial metabolic inhibitor. In support, immunohistochemical analysis revealed activation of dorsal horn astrocytes and microglia after intraplantar injection of either sPLA2. Spinal proinflammatory cytokines, nitric oxide, and prostanoids each appear to be involved in the pain-enhancing effects of these sPLA2s. Blockade of interleukin-1 (IL1) inhibited hyperalgesia induced by both sPLA2s, while leaving allodynia unaffected. Blockade of tumor necrosis factor reduced responses to sPLA2-Asp49. An inhibitor of neuronal nitric oxide synthase, 7-nitroindazole (7-NI), inhibited hyperalgesia induced by both sPLA2s, without interfering with allodynia induced by sPLA2-Asp49. On the other hand, L-N(6)-(1-iminoethyl)lysine (L-NI), an inhibitor of the inducible nitric oxide synthase, did not alter any sPLA2-induced effect. Lastly, celecoxib, an inhibitor of cyclooxygenase-2, attenuated sPLA2 actions. These data provide the first evidence of spinal mediators involved in pain facilitation induced by subcutaneous venoms.
journal_name
Painjournal_title
Painauthors
Chacur M,Gutiérrez JM,Milligan ED,Wieseler-Frank J,Britto LR,Maier SF,Watkins LR,Cury Ydoi
10.1016/j.pain.2004.06.001keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
65-76issue
1-2eissn
0304-3959issn
1872-6623pii
S0304395904002787journal_volume
111pub_type
杂志文章相关文献
PAIN文献大全abstract：:It has been suggested that a number of peptides may be involved in the transmission of pain. In order to evaluate the possible role of peptides in the development of headache, we have, in the present study, examined the presence of nerve fibres containing neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), subs...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/0304-3959(86)90162-4
更新日期：1986-12-01 00:00:00
abstract：:Chronic complications of traumatic brain injury represent one of the greatest financial burdens and sources of suffering in the society today. A substantial number of these patients suffer from posttraumatic headache (PTH), which is typically associated with tactile allodynia. Unfortunately, this phenomenon has been u...
journal_title：Pain
pub_type： 杂志文章
doi：10.1097/j.pain.0000000000002103
更新日期：2020-10-02 00:00:00
abstract：:In the present study the question was addressed whether sensitivity to experimental pain stimuli differs between families, which are previously characterized by the degree of cold tolerance (very insensitive or very sensitive) of one family member. A total of 232 healthy medical students were screened for cold pain to...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2007.11.012
更新日期：2008-08-15 00:00:00
abstract：:Human and animal imaging studies demonstrated that chronic pain profoundly alters the structure and the functionality of several brain regions. In this article, we conducted a longitudinal and multimodal study to assess how chronic pain affects the brain. Using the spared nerve injury model which promotes both long-la...
journal_title：Pain
pub_type： 杂志文章
doi：10.1097/j.pain.0000000000001610
更新日期：2019-10-01 00:00:00
abstract：:We have previously reported a novel method for producing chronic nociceptive behavior in rats following compression of the trigeminal ganglion. In the present study, we have further studied the role of demyelination in the development of prolonged nociceptive behavior in the trigeminal territory. For this purpose, lys...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2009.07.012
更新日期：2009-11-01 00:00:00
abstract：:A psychophysical investigation was carried out to examine whether heat hyperalgesia exists within the secondary mechanical hyperalgesia zone surrounding a capsaicin injection site on hairy skin. A non-contact laser stimulator was used to deliver temperature controlled stimuli to sites within and outside the zone of me...
journal_title：Pain
pub_type： 临床试验,杂志文章
doi：10.1016/s0304-3959(96)03199-5
更新日期：1996-12-01 00:00:00
abstract：:To quantify performance differences between patients with low-back pain (LBP) and a control group during their performance of a repetitive isodynamic lifting task. Case-control study was done. LBP patients were recruited and tested at an outpatient ambulatory chronic pain rehabilitation program before treatment was be...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/s0304-3959(03)00247-1
更新日期：2003-09-01 00:00:00
abstract：:VGF (nonacronymic) is a granin-like protein that is packaged and proteolytically processed within the regulated secretory pathway. VGF and peptides derived from its processing have been implicated in neuroplasticity associated with learning, memory, depression, and chronic pain. In sensory neurons, VGF is rapidly incr...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2014.03.012
更新日期：2014-07-01 00:00:00
abstract：:Substance P (SP) is released from primary afferent fibers in response to nociceptive stimuli. Capsaicin, which produces an initial hyperalgesic response followed by persistent antinociception, also elicits release of SP from primary afferent fibers. Capsaicin pretreatment decreases the content and release of SP from p...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/s0304-3959(97)03376-9
更新日期：1997-07-01 00:00:00
abstract：:It is known that morphine has a negative impact on the immune responses. The potent opioids fentanyl and buprenorphine have recently become available as transdermal preparation for the treatment of chronic pain. We analyze the effect of fentanyl and buprenorphine on splenic cellular immune responses in the mouse. The ...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2004.04.020
更新日期：2004-07-01 00:00:00
abstract：:Cyclooxygenase-2 (COX-2) activity in the spinal cord plays a key role in sensitization to sensory stimuli during acute inflammation. In contrast, intrathecal administration of COX-2 specific inhibitors has minimal analgesic effects in an incisional model of postoperative pain. We investigated the role of COX isoforms ...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/s0304-3959(02)00465-7
更新日期：2003-07-01 00:00:00
abstract：:The treatment of neuropathic pain by neuromodulation is an objective for more than 40 years in modern clinical practice. With respect to spinal cord and deep brain structures, the cerebral cortex is the most recently evaluated target of invasive neuromodulation therapy for pain. In the early 90s, the first successes o...
journal_title：Pain
pub_type： 杂志文章,评审
doi：10.1097/j.pain.0000000000000401
更新日期：2016-02-01 00:00:00
abstract：:This study focuses on changes in adrenergic sensitivity in untransected sensory axons that innervate an area of skin made neuropathic by transection of neighboring nerves. The segmental nerve injury model is favorable for this since all axons in the L5 and L6 nerves are transected whereas the L4 axons are intact. Earl...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/S0304-3959(98)00252-8
更新日期：1999-04-01 00:00:00
abstract：:In recent years, oxytocin has been implicated in a wide diversity of functions. The role of oxytocin in analgesia and pain modulation represents an important new function of an endogenous system controlling sensorial information. The paraventricular (PV) nucleus of the hypothalamus is one of the most important sources...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2006.01.029
更新日期：2006-05-01 00:00:00
abstract：:Patients with schizophrenia report reduced pain sensitivity in clinical studies, but experimental studies are required to establish pain sensitivity as a potential endophenotype. We conducted a systematic review of electronic databases from database inception until April 15, 2015, including experimental studies invest...
journal_title：Pain
pub_type： 杂志文章,meta分析
doi：10.1097/j.pain.0000000000000304
更新日期：2015-11-01 00:00:00
abstract：:Though it is clear that genomic variability plays an integral role in accounting for pain sensitivity, controversy exists over which genes are involved. While recent data suggest a "protective" (i.e., less pain) haplotype in the GTP cyclohydrolase (GCH1) gene, other research has failed to confirm this association. Pos...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2008.10.023
更新日期：2009-01-01 00:00:00
abstract：:Intramedullary or subarachnoid injections of alumina cream were made in the lumbar region of 38 adult cats. The animals were observed for 3 to 12 months and then subjected to acute neurophysiologic and histologic analysis. Neuronal hyperactivity in the dorsal horns was reliably produced by either subarachnoid or intra...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/0304-3959(78)90011-8
更新日期：1978-10-01 00:00:00
abstract：:Functional magnetic resonance imaging (fMRI) using the blood oxygen level-dependent (BOLD) contrast was used to study sensory processing in the brain of isoflurane-anesthetized mice. The use of a cryogenic surface coil in a small animal 9.4T system provided the sensitivity required for detection and quantitative analy...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2010.08.025
更新日期：2010-12-01 00:00:00
abstract：:Patients with chronic pain often report being sensitive to pain at night before falling asleep, a time when the synchronization of cortical activity is initiated. However, how cortical activity relates to pain sensitivity is still unclear. Because sleep is characterized by enhanced cortical delta power, we hypothesize...
journal_title：Pain
pub_type： 杂志文章
doi：10.1097/j.pain.0000000000001725
更新日期：2020-02-01 00:00:00
abstract：:Poor women and women of color are disproportionately affected by HIV and this is particularly evident in sub-Saharan Africa. Even with increased access to antiretroviral therapy that makes HIV/AIDS a chronic manageable condition, little progress has been made to address the issues of pain caused by HIV disease in wome...
journal_title：Pain
pub_type： 杂志文章,评审
doi：10.1016/j.pain.2007.10.009
更新日期：2007-11-01 00:00:00
abstract：:P2X3 purinoceptor cellular distribution was studied in rat sensory neurons in naive animals and following peripheral nerve injury using immunohistochemical methods. Specific antiserum was raised in rabbits and characterized by Western blot, absorption assays and labeling of recombinant receptors. In naive animals, P2X...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/s0304-3959(98)00225-5
更新日期：1999-03-01 00:00:00
abstract：:There is extensive evidence that spinal excitatory amino acids (EAAs) like glutamate (Glu) and aspartate (Asp) are important in the processing of nociceptive behaviors caused by incisions. To better understand EAA-induced dorsal horn sensitization caused by surgery, we examined the time course and extent of spinal ami...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/s0304-3959(02)00241-5
更新日期：2002-11-01 00:00:00
abstract：:A comprehensive treatment program for chronic disability related to back disease has been presented. This program has used not only more traditional methods of medical care for the structural disabilities of chronic mechanical back disorders, but has used principles of active patient participation in the improvement p...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/0304-3959(76)90007-5
更新日期：1976-09-01 00:00:00
abstract：:We report findings from clinical examination and cutaneous laser stimulation in a 57-year-old male, who suffered from a right-sided postcentral stroke. In this patient, we were able to demonstrate (i) a dissociation of discriminative and affective components of pain perception and, for the first time in humans, (ii) t...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/s0304-3959(99)00012-3
更新日期：1999-05-01 00:00:00
abstract：:Analgesic response, incidental pain and pain mechanism were evaluated in order to predict the chances of pain relief in advanced cancer patients. In an observation period of about 2 months, 130 patients were followed at home until death using only a pharmacological approach to control pain according to the WHO ladders...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/0304-3959(92)90155-5
更新日期：1992-08-01 00:00:00
abstract：:Ten patients with myofascial trigger point pain were entered into a double-blind cross-over study of the reversibility of myofascial trigger point injection (TPI) effects with naloxone versus placebo in order to test the hypothesis that the benefits of TPI are mediated, at least in part, through activation of an endog...
journal_title：Pain
pub_type： 临床试验,杂志文章
doi：10.1016/0304-3959(88)90018-8
更新日期：1988-01-01 00:00:00
abstract：:The facial expression of pain plays a crucial role in pain communication and pain diagnostics. Despite its importance, it has remained unknown which dimensions of pain (sensory and/or affective) are encoded in the face. To answer this question, we used a well-established cognitive strategy (suggestions) to differentia...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2011.10.027
更新日期：2012-02-01 00:00:00
abstract：:Facial expressions of pain and emotions provide powerful social signals, which impart information about a person's state. Unfortunately, research on pain and emotion expression has been conducted largely in parallel with few bridges allowing for direct comparison of the expressive displays and their impact on observer...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2007.05.008
更新日期：2008-03-01 00:00:00
abstract：:Data on comparative safety of opioid analgesics are limited, but some reports suggest disproportionate mortality risk associated with methadone. Our objective was to compare mortality rates among patients who received prescribed methadone or long-acting morphine for pain. This is a retrospective observational cohort d...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/j.pain.2011.03.023
更新日期：2011-08-01 00:00:00
abstract：:An experimental mononeuropathy was produced in rats by unilateral loose ligation of the sciatic nerve, with a contralateral sham surgery. Repeated bilateral sensory testing was performed over time to investigate any threshold differences between the loose ligature side and the contralateral control side. A prolonged r...
journal_title：Pain
pub_type： 杂志文章
doi：10.1016/0304-3959(93)90004-9
更新日期：1993-12-01 00:00:00
