Abstract:
:There is extensive evidence that spinal excitatory amino acids (EAAs) like glutamate (Glu) and aspartate (Asp) are important in the processing of nociceptive behaviors caused by incisions. To better understand EAA-induced dorsal horn sensitization caused by surgery, we examined the time course and extent of spinal amino acid (AA) release during and after a plantar incision utilizing in vivo microdialysis. We also examined the role of primary afferent input and axonal conduction by measuring spinal EAAs in rats after hindpaw denervation and in rats treated with spinal tetrodotoxin (TTX). In halothane-anesthetized rats, a microdialysis filament (200 microm diameter, 45,000 MW cut off, Hospal AN69) was passed transversely through the deep dorsal horn of the spinal cord (L4-L6). After 18-24h, the dialysis filament was perfused with artificial cerebrospinal fluid (ACSF), dialysate samples collected and analyzed for AAs (Glu, Asp, asparagine, glutamine, serine, and glycine). Rats underwent anesthetic induction with halothane followed by a plantar incision (n=8) or sham operation (n=8). AAs were also measured in incised rats that underwent hindpaw denervation (n=4), in rats that had the filament placed outside the L4-L6 spinal segments (n=7), in rats with a microdialysis catheter placed in the ventral horn at L4-L6 (n=7) and in rats treated with spinally administered TTX (n=5). AAs were measured during recovery from anesthesia and for the next 8h. In the sham-operated group, Asp and Glu did not change throughout the experiment. In rats undergoing plantar incision, Asp and Glu increased from 10 to 30 min after incision to 200+/-30 and 138+/-12 percentage of control, respectively. The EAAs returned to baseline by 1h after incision. For the other AAs, only serine and asparagine increased after incision. No increase in AA release by incision was observed after hindpaw denervation, TTX treatment or placement of the filament outside the L4-L6 segments. In rats with a filament implanted in the ventral horn (L4-L6), EAAs increased during halothane induction and sham preparation. Thus, the EAA release required an intact afferent nerve barrage and segmental excitatory nerve transmission. The incision-induced nociceptive afferent barrage increased the release of Glu and Asp in the lumbar dorsal horn for 45 min. The concentrations of AAs returned to baseline by 1h. The percentage increase is in some cases less and for a shorter period of time compared to other models of persistent pain, perhaps because the incision injury is less severe compared to others models. This profile of EAA release further explains why models of inflammation and chemical irritation do not translate well to human postoperative pain.
journal_name
Painjournal_title
Painauthors
Zahn PK,Sluka KA,Brennan TJdoi
10.1016/s0304-3959(02)00241-5keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
65-76issue
1-2eissn
0304-3959issn
1872-6623pii
S0304395902002415journal_volume
100pub_type
杂志文章相关文献
PAIN文献大全abstract::Do contrasting neuropathic pain diagnoses share common pathophysiological mechanisms? Selective breeding was used to derive rat lines with a common genetic background but a striking difference in the degree of spontaneous pain behavior expressed in the neuroma model of neuropathic pain (HA rats (high autotomy) and LA ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2009.07.020
更新日期:2009-11-01 00:00:00
abstract::The characteristics and impact of pain were evaluated in a prospective cross-sectional survey of 438 ambulatory AIDS patients recruited from health care facilities in New York City. More than 60% of the patients reported 'frequent or persistent pain' during the 2 wks preceding the study. Patients with pain reported an...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/s0304-3959(96)03215-0
更新日期:1996-12-01 00:00:00
abstract::The treatment of neuropathic pain by neuromodulation is an objective for more than 40 years in modern clinical practice. With respect to spinal cord and deep brain structures, the cerebral cortex is the most recently evaluated target of invasive neuromodulation therapy for pain. In the early 90s, the first successes o...
journal_title:Pain
pub_type: 杂志文章,评审
doi:10.1097/j.pain.0000000000000401
更新日期:2016-02-01 00:00:00
abstract::This research was designed to identify and validate facial expressions of pain occurring in a clinical chronic pain condition. Facial expressions of 28 patients with chronic temporomandibular disorder pain were videotaped during a painful clinical examination procedure and coded using an anatomically based system. Cha...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(88)90278-3
更新日期:1988-10-01 00:00:00
abstract::Genetic influence on the sensory, motor and histopathological outcome of transient spinal cord ischemia was studied in Sprague-Dawley (SD), spontaneously hypertensive (SH) and Wistar-Kyoto (WKY) rats. Ischemia caused minor motor deficits and no identifiable morphological damage in the spinal cord of the majority of SD...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(93)90152-F
更新日期:1993-11-01 00:00:00
abstract::This study was designed to investigate the relative efficacy of cognitive-behavioral group treatment, including relaxation training, in comparison with a control condition in a sample of 20 outpatients with chronic low back pain. Subjects in both conditions also received the same physiotherapy back-education and exerc...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(92)90082-M
更新日期:1992-03-01 00:00:00
abstract::Migraine is a common and disabling neurologic disorder, with important psychiatric comorbidities. Its pathophysiology involves activation of neurons in the trigeminocervical complex (TCC). Kainate receptors carrying the glutamate receptor subunit 5 (GluK1) are present in key brain areas involved in migraine pathophysi...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/01.j.pain.0000460325.25762.c0
更新日期:2015-03-01 00:00:00
abstract::In recent years, oxytocin has been implicated in a wide diversity of functions. The role of oxytocin in analgesia and pain modulation represents an important new function of an endogenous system controlling sensorial information. The paraventricular (PV) nucleus of the hypothalamus is one of the most important sources...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2006.01.029
更新日期:2006-05-01 00:00:00
abstract::Endogenous pain inhibition is less efficient in chronic pain patients. Diffuse noxious inhibitory control (DNIC), a form of endogenous pain inhibition, is compromised in women and older people, making them more vulnerable to chronic pain. However, the underlying mechanisms remain unclear. Here, we used a capsaicin-ind...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000001810
更新日期:2020-06-01 00:00:00
abstract::Recent meta-analyses have shown mindfulness-based interventions (MBIs) to be effective for chronic pain, but no pooled estimates of the effect of MBIs on acute pain are available. This meta-analysis was conducted to fill that gap. A literature search was conducted in 4 databases. Articles were eligible if they reporte...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000001877
更新日期:2020-08-01 00:00:00
abstract::Twenty patients with chronic low back pain received 12 lumbar sympathetic injections, in a series of 6 with bupivacaine and a series of 6 with saline. Changes in subjective pain intensity, EMG from paravertebral muscles, joint ranges of mobility, and daily activity levels were measured at multiple intervals throughout...
journal_title:Pain
pub_type: 临床试验,杂志文章
doi:10.1016/0304-3959(80)90085-8
更新日期:1980-02-01 00:00:00
abstract::Unilateral intraplantar injection of Freund's complete adjuvant (FCA) into 1 hind paw of rats was used as a model of peripheral inflammation and persistent pain in order to examine time course effects of a continuous barrage of nociceptive input on the second-messenger transducing systems in the spinal cord. cAMP, cGM...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(94)90185-6
更新日期:1994-07-01 00:00:00
abstract::Chronic neuropathic pain is associated with long-term changes at multiple levels of the neuroaxis, including in the brain, where electrical stimulation has been used to manage severe pain conditions. However, the clinical outcome of deep brain stimulation is often mixed, and the mechanisms are poorly understood. By me...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2011.07.011
更新日期:2011-11-01 00:00:00
abstract::Few surveys have been performed to define the characteristics and impact of breakthrough pain in the cancer population. In this cross-sectional survey of inpatients with cancer, patients responded to a structured interview (the Breakthrough Pain Questionnaire) designed to characterize breakthrough pain, and also compl...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/s0304-3959(99)00006-8
更新日期:1999-05-01 00:00:00
abstract::The purpose of this study was to assess if the functional activation caused by painful stimuli could be detected with arterial spin labeling (ASL), which is a non-invasive magnetic resonance imaging (MRI) technique for measuring cerebral blood flow (CBF). Because ASL directly measures blood flow, it is well suited to ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2007.06.021
更新日期:2008-05-01 00:00:00
abstract::The capsaicin receptor in nociceptive neurons is a target for the sensitizing actions of algogenic inflammatory mediators. Capsaicin and potential endogenous ligands are thought not to gate this heat-activated ion channel but to sensitize it so profoundly that even room temperature can open it. We investigated the tem...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2004.03.033
更新日期:2004-07-01 00:00:00
abstract::Activation of supraspinal gamma-aminobutyric acid-A (GABAA) receptors is known to result in antagonism of opioid analgesia. Since benzodiazepines enhance the action of GABA at GABAA receptors, we hypothesized that administration of these agents for preoperative sedation might antagonize the analgesic effects of opioid...
journal_title:Pain
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0304-3959(97)03332-0
更新日期:1997-05-01 00:00:00
abstract::Behavioral studies have suggested that placebo analgesia is partly mediated by the endogenous opioid system. Expanding on these results we have shown that the opioid-receptor-rich rostral anterior cingulate cortex (rACC) is activated in both placebo and opioid analgesia. However, there are also differences between the...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2010.03.011
更新日期:2010-07-01 00:00:00
abstract::Intrathecal (i.t.) administration of spermine (0.1-10000 fmol), an endogenous polyamine, produced the behavioural response mainly consisting of biting and/or licking of the hindpaw along with a slight hindlimb scratching directed toward the flank in mice, which peaked at 5-15 min and almost disappeared at 30 min after...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/s0304-3959(99)00312-7
更新日期:2000-05-01 00:00:00
abstract::The responses of primate spinothalamic tract (STT) neurons to innocuous and noxious mechanical stimuli applied to the skin can be enhanced for more than an hour following prolonged noxious stimulation. This increased responsiveness is thought to reflect sensitization of dorsal horn neurons and may help account for sec...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(94)90164-3
更新日期:1994-03-01 00:00:00
abstract::A chronic compression of the dorsal root ganglion (CCD) produces ipsilateral cutaneous hyperalgesia and allodynia in rats. Intracellular electrophysiological recordings from formerly compressed neurons in the intact dorsal root ganglion (DRG) reveal lower than normal current thresholds (CTs) and abnormal spontaneous a...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2004.10.001
更新日期:2005-01-01 00:00:00
abstract:ABSTRACT:Spinal cord stimulation (SCS) is an approved treatment for truncal and limb neuropathic pain. However, pain relief is often suboptimal and SCS efficacy may reduce over time, requiring sometimes the addition of other pain therapies, stimulator revision, or even explantation. We designed and tested a new procedu...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000002160
更新日期:2020-11-30 00:00:00
abstract::Endogenous TRPV1 agonists such as oxidized linoleic acid metabolites (OLAMs) and the enzymes releasing them [eg, cytochrome P450 (CYP)] are up-regulated after inflammation in the rat. However, it is not known whether such agonists are elevated in human inflammatory pain conditions. Because TRPV1 is expressed in human ...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2013.07.011
更新日期:2013-11-01 00:00:00
abstract::In a double-blind, cross-over study, ibuprofen (600 mg), a peripherally-acting selective kappa-opioid receptor agonist (7.5 mg), or placebo were given orally in experiments on healthy volunteers 1 h before assessment of pain thresholds to radiant heat and of pain ratings to controlled mechanical impact stimuli. Mechan...
journal_title:Pain
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/S0304-3959(98)00062-1
更新日期:1998-06-01 00:00:00
abstract::Skin conductance and heart rate measures obtained from subjects previous to, during, and after their performance of the cold pressor task were correlated with their responses to the McGill Pain Questionnaire administered immediately after the completion of the cold pressor task. A reliable relationship between skin co...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/0304-3959(82)90146-4
更新日期:1982-01-01 00:00:00
abstract::Pain cannot be directly measured in neonates. Therefore, scores based on indirect behavioural signals such as crying, or physiological signs such as blood pressure, are used to quantify neonatal pain both in clinical practice and in clinical studies. The aim of this study was to determine which of the physiological an...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000000485
更新日期:2016-08-01 00:00:00
abstract::The social determinants of health (SDH) are known to differentially impact outcomes from many noncommunicable diseases; however, their potential role in low back pain (LBP) is poorly defined. This review endeavours to comprehensively inform the field of their relevance. Our research question was: "How do the broad ran...
journal_title:Pain
pub_type: 杂志文章
doi:10.1097/j.pain.0000000000001944
更新日期:2020-11-01 00:00:00
abstract::The objective of this study was to examine which factors during pregnancy and postpartum predict pelvic girdle pain (PGP) at 12 weeks postpartum among working women. A total of 548 Dutch pregnant employees were recruited in 15 companies, mainly health care, child care, and supermarkets. The definition of PGP was any p...
journal_title:Pain
pub_type: 杂志文章,多中心研究
doi:10.1016/j.pain.2012.08.003
更新日期:2012-12-01 00:00:00
abstract::The behavioral response to acute tissue injury is usually characterized by different phases, but the brain mechanisms underlying changes in pain-related behavior over time are still poorly understood. We aimed to analyze time-dependent changes in metabolic activity levels of 49 forebrain structures in the formalin pai...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/s0304-3959(03)00015-0
更新日期:2003-07-01 00:00:00
abstract::Recent studies showed an enhanced general sensitivity to painful stimuli in adult migraineurs during as well as between attacks. Yet, the influence of a prolonged pain history and potential sex differences has not been studied. We used quantitative sensory testing to examine 25 children with migraine between attacks a...
journal_title:Pain
pub_type: 杂志文章
doi:10.1016/j.pain.2005.12.015
更新日期:2006-07-01 00:00:00