Abstract：
:Proteomic analysis is defined as the characterization of the entire set of proteins encoded by a genome. Two-dimensional (2D) electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) are key technologies used in proteomic analysis to gain information about protein expression profiles and post-translational modifications. Knowledge about aging processes can be gained by recognizing changes in protein expression. Thus, to better understand the aging process through protein profiling, post-mitochondrial (PM) fractions of young (13-month) and old (31-month) male Fischer 344 rat kidney were differentially analyzed by 2D. We detected a total number of 380 spots on 2D gel images. Among them, 167 spots showed 2-fold significant alterations (p<0.05) between young and old PM fractions. Further, 103 proteins were identified by MALDI-TOF MS. The PM fraction of aged rat kidney showed increases in antioxidative and proteolytic proteins and decreases in cytoskeletal proteins. In addition, we found age-related changes in transport and homeostasis proteins. Thus, our results demonstrated that proteomic analysis can be effectively applied to the assessment of the age status of protein expression, and thereby provide valuable information on age-related changes of proteome.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Kim CH,Park DU,Chung AS,Zou Y,Jung KJ,Sung BK,Yu BP,Chung HYdoi
10.1016/j.exger.2004.04.003keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
1155-68issue
8eissn
0531-5565issn
1873-6815pii
S0531556504001731journal_volume
39pub_type
杂志文章abstract：:Gene expression is much altered in aging. We observed age-dependent decline of core clock genes' expression in the whole body of the fruit fly. We hypothesized that inducible overexpression of clock genes (cry, per, tim, cyc and Clk) in the nervous system can improve healthspan of D. melanogaster. We studied the lifes...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2018.11.005
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:The contribution of the A3243G mutation in mitochondria DNA (mtDNA) to diabetes mellitus (DM) in Werner's syndrome (WS) was studied. PATIENTS AND METHOD:DNA samples from peripheral white blood cells (WBCs) originating from 24 Japanese WS patients aged 30-56 were used. For control, 239 subjects aged 15-95 wer...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(02)002090-7
更新日期：2003-03-01 00:00:00
abstract：BACKGROUNDS:Elucidating the causal effects of common intermediate risk factors on the onset of age-related diseases is indispensable for developing prevention and intervention procedures. METHODS:We conducted two-stage time-to-event Mendelian randomization meta-analyses combining five large-scale longitudinal cohorts ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2017.09.019
更新日期：2018-07-01 00:00:00
abstract：:Natural killer (NK) cells play an important role in controlling several viral diseases. Our previous studies demonstrated an age-dependent susceptibility to mousepox due to defective NK cell responses and trafficking. However, the mechanisms that underlie the age-related impairment in NK cell migration have yet to be ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2017.06.021
更新日期：2017-10-01 00:00:00
abstract：:Neuroinflammation may play a role in the pathogenesis of Parkinson's disease (PD). The present study questioned whether this neuroinflammatory response differs between the olfactory bulb, as an early affected region and the nigrostriatal system. Indeed, increased microgliosis was shown in post-mortem olfactory bulb of...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2007.04.010
更新日期：2007-08-01 00:00:00
abstract：:Aging is associated with altered immune responses, particularly with a diminished CD8 T cell response. Although both intrinsic and extrinsic factors are hypothesized to impact this decreased T cell response, the direct evidence of an intrinsic deficiency in virus-specific CD8 T cells is limited. In this study, a TCR t...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2013.02.027
更新日期：2013-06-01 00:00:00
abstract：:We investigated the telomere lengths of individual cell types in lingual mucosa using an improved tissue quantitative fluorescence in situ hybridization (Q-FISH) method. Our tissue Q-FISH method compensates for partially cut nuclei in a tissue section by using the telomere:centromere ratio (TCR). We normalized our TCR...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2008.06.001
更新日期：2008-09-01 00:00:00
abstract：:We have recently reported that activated mouse spleen mononuclear cells (MNCs) efficiently lyse autologous erythrocytes in vitro (Saxena and Chandrasekhar, 2000). In the present study, we have investigated erythrocyte-depleting ability (EDA) of spleen MNCs from young and old mice. Time kinetics of survival of erythroc...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(00)00091-7
更新日期：2000-05-01 00:00:00
abstract：:There is growing concern over the increasing instances of decline in cognitive abilities with aging in humans. The present study evaluated the benefits of the natural antioxidant, grape seed proanthocyanidin extract (GSPE) in treating the effects of age-related oxidative stress (OS) and accumulation of lipofuscin (LF)...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2011.08.006
更新日期：2011-11-01 00:00:00
abstract：:The field of toxicology adopted the threshold dose response in the early decades of the 20th century. The model was rapidly incorporated into governmental regulatory assessment procedures and became a central feature of chemical evaluation and assessment. The toxicological community never validated the capacity of thi...
journal_title：Experimental gerontology
pub_type： 历史文章,杂志文章
doi：10.1016/j.exger.2012.02.004
更新日期：2013-01-01 00:00:00
abstract：:Given the increased concerns about the degenerative decline in the physical performance of the elderly, there is a need for developing effective strategies to suppress the age-related loss of skeletal muscle mass and functional capacity through a lifestyle intervention. This randomized controlled trial examined whethe...
journal_title：Experimental gerontology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.exger.2016.11.003
更新日期：2017-01-01 00:00:00
abstract：:Diet is the most variable aspect of life history, as most individuals have a large diversity of food choices, varying in the type and amount that they ingest. In the short-term, diet can affect metabolism and energy levels. However, in the long run, the net deficiency or excess of calories from diet can influence the ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.02.012
更新日期：2016-12-15 00:00:00
abstract：:Long-term caloric restriction (CR) has been shown to extend maximum life span in laboratory rodents. We investigated the activities of gluconeogenic and transaminase enzymes in the livers of old and young mice fed either control or calorie-restricted diets. Livers were sampled 48 h after the last scheduled feeding tim...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(02)00202-4
更新日期：2003-03-01 00:00:00
abstract：:During the course of life, cyclic females face a state of midlife transition that occurs in a fully functioning neurological system, and results in reproductive senescence. The authors' hypothesis was that changes in the activity noradrenergic neurons may be one of the factors involved in this phenomenon. The aim of t...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.04.015
更新日期：2016-08-01 00:00:00
abstract：:A colony of male Lakeview Golden (LVG) Syrian hamsters has been maintained for the last nine years as a source of various tissues for cellular aging studies. Observations on this colony also yielded data on survival time and physical and pathological manifestations of aging in this strain. Based on 150 spontaneous dea...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(90)90032-w
更新日期：1990-01-01 00:00:00
abstract：:Atrophy of the thymus, the primary site of T lymphocyte generation, is a hallmark of the aging immune system. Age-associated thymic atrophy results in diminished output of new, naïve T cells, with immune sequelae that include diminished responses to novel pathogenic challenge and vaccines, as well as diminished tumor ...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2017.12.022
更新日期：2018-05-01 00:00:00
abstract：:Mitochondria play a critical role in the life of the cell as they control their metabolic rate, energy production and cell death. Mitochondria have long been appreciated as causative to aging. The age-associated respiratory chain deficiency is typically unevenly distributed and affects only a subset of cells in variou...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/j.exger.2008.05.006
更新日期：2009-01-01 00:00:00
abstract：:Fish provide enormous spectrum of longevity and thus present the possibility of multiple mechanisms of senescence. Oxidative stress as a causative agent of senescence and the protective role of antioxidant enzymes were tested in the teleost, Channa punctatus taking peroxidase (POD) (EC 1.1 1.1.7) as the representative...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(99)00021-2
更新日期：1999-06-01 00:00:00
abstract：:Despite well-documented evidence for lifespan extension by methionine restriction (MR), underlying mechanisms remain unknown. As methionine can alter S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH), the substrate and product of DNA methyltransferase-1 (DNMT1), we hypothesized that MR diet alters DNA methyl...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.12.003
更新日期：2017-02-01 00:00:00
abstract：:This article addresses the description of several endocrine-related functions of the brain. It has emerged that steroids and growth factors may influence brain functions, and that brain cells may metabolize sex steroids. The crosstalk between different types of brain cells (neurons, astrocytes, the LHRH (luteinizing h...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/s0531-5565(96)00172-6
更新日期：1997-07-01 00:00:00
abstract：:Progressive neuronal deterioration accompanied by sensory functions decline is typically observed during aging. On the other hand, structural or functional alterations of specific sensory neurons extend lifespan in the nematode Caenorhabditis elegans. Hormesis is a phenomenon by which the body benefits from moderate s...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.03.026
更新日期：2014-08-01 00:00:00
abstract：:An age-related increase of DNA damage/mutation has been previously reported in human lymphocytes. The high copy number and mutation rate make the mtDNA genome an ideal candidate for assessing damage and to act as a potential biomarker of ageing. In the present study, two assays were developed to evaluate the level of ...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/s0531-5565(01)00200-5
更新日期：2002-01-01 00:00:00
abstract：:Reactive oxygen species (ROS) are formed in all living organisms as a by-product of normal metabolism (endogenous sources) and as a consequence of exposure to environmental compounds (exogenous sources). Endogenous ROS are largely formed during oxidative phosphorylation in the mitochondria and, therefore, mitochondria...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/s0531-5565(02)00142-0
更新日期：2002-10-01 00:00:00
abstract：:Osteoporosis is extremely frequent in post-menopausal women; nevertheless, osteoporosis in men is also a severe and frequently occurring but often underestimated disease. Increasing evidence links bone loss in male idiopathic osteoporosis and age related osteoporosis to osteoblast dysfunction rather than increased ost...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2014.05.014
更新日期：2014-09-01 00:00:00
abstract：:Average life expectancy at birth is a rough measure of the span of healthy, productive life--the functional life span. In the developed countries average life expectancies at birth now range from 76-79 years, six to nine years less than the limit of about 85 years imposed by aging. Aging is the accumulation of changes...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/s0531-5565(97)00059-4
更新日期：1998-01-01 00:00:00
abstract：:Rats of Albino Oxford (AO) strain in our animal facility exhibit a longer average healthy life span than rats of Dark Agouit (DA) strain. Since chronic activation of macrophages contributes to chronic low level inflammation common in older age, elucidation of the changes in middle-aged rats could be useful in preventi...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2016.10.005
更新日期：2016-12-01 00:00:00
abstract：:The loss of bone mineral with aging and subsequent development of osteoporosis is a common problem in elderly women, and as life expectancy increases, in elderly men as well. Space flight also causes bone loss and could be a limiting factor for long duration missions, such as, a Mars expedition or extended occupation ...
journal_title：Experimental gerontology
pub_type： 杂志文章,评审
doi：10.1016/0531-5565(91)90011-a
更新日期：1991-01-01 00:00:00
abstract：:The aim of this study was to compare the functional and structural changes in similarly identified neurons of the small parietal ganglion in 56 molluscs (Lymnaea stagnalis) of two age groups: adult (10-12 months) and old (20-22 months). No age changes were found in the values of membrane potential, resistance of the n...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/0531-5565(95)00008-5
更新日期：1995-09-01 00:00:00
abstract：:Telomeres serve as a mitotic clock and biological marker of senescence. Diabetes mellitus (DM) is associated with damage to target organs and premature aging. We assessed the effect of glycemic control on telomere dynamics in arterial cells of 58 patients undergoing coronary artery bypass and in mononuclear blood cell...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2007.07.005
更新日期：2007-10-01 00:00:00
abstract：:SAMP8 mice exhibit multiple metabolic characteristics associated with age, and it is a suitable candidate for researching aging associated metabolic dysfunction. OBJECTIVES:We aimed to 1) explore how key metabolic markers will be altered in both liver and adipose tissue with aging in SAMP8 mice; and 2) how the combin...
journal_title：Experimental gerontology
pub_type： 杂志文章
doi：10.1016/j.exger.2017.03.017
更新日期：2017-07-01 00:00:00
