Abstract：
:Vascular endothelial growth factor (VEGF) is a principal stimulator of angiogenesis. However, the downstream targets of VEGF in endothelial cells (ECs) are not entirely clarified. Survey of downstream targets of VEGF in human ECs identified a number of genes, including Down syndrome candidate region 1 (DSCR1). Here, we confirmed the inducible expression of DSCR1 in ECs by Northern and Western blottings. Moreover, VEGF-stimulated induction of DSCR1 was blocked by anti-VEGF receptor-2 monoclonal antibody (mAb), or the specific calcineurin inhibitors cyclosporin A and FK506. The expression of DSCR1 in ECs of neovessels was further shown by immunohistochemical analysis. We therefore examined whether DSCR1 played any roles in angiogenesis. The specific downregulation of DSCR1 expression by antisense oligonucleotide (AS-ODN) inhibited VEGF-stimulated migration of ECs as well as angiogenesis in vivo. AS-ODN inhibited the spreading of ECs on vitronectin, as well as on the immobilized anti-alphavbeta3 mAb, but not on anti-alphavbeta5 mAb. Moreover, AS-ODN inhibited tyrosine phosphorylation of focal adhesion kinase when ECs were plated on a vitronectin-coated dish. Immunoprecipitation followed by Western blotting showed the coimmunoprecipitation of DSCR1 and integrin alphavbeta3. These results suggest that DSCR1 is involved in angiogenesis by regulating adhesion and migration of ECs via the interaction with integrin alphavbeta3.
journal_name
J Vasc Resjournal_title
Journal of vascular researchauthors
Iizuka M,Abe M,Shiiba K,Sasaki I,Sato Ydoi
10.1159/000079832keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
334-44issue
4eissn
1018-1172issn
1423-0135pii
79832journal_volume
41pub_type
杂志文章abstract：:The novel endothelial cell tyrosine kinase receptors, Tie1 and Tie2, are essential for vascular development and remodeling in the embryo but little is known regarding the regulation of their expression and their role in the maintenance and repair of the adult vascular system. We examined the expression of Tie1 and Tie...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025655
更新日期：1999-07-01 00:00:00
abstract：:This study utilised catecholamine fluorescence histochemistry, multiple-labelling immunohistochemistry and retrograde axonal transport to determine the distribution and neuropeptide content of sympathetic neurons innervating veins in the dorsal skin of the guinea pig pinna. There was a dense plexus of sympathetic axon...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159113
更新日期：1995-11-01 00:00:00
abstract：UNLABELLED:Diabetic patients have an increased risk of restenosis and late stent thrombosis after angioplasty, i.e. complications that are related to a defective re-endothelialization. Exendin-4, a stable glucagon-like peptide (GLP)-1 receptor agonist, has been suggested to influence the formation of intimal hyperplasi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000381097
更新日期：2015-01-01 00:00:00
abstract：:Peroxynitrite is an endothelium-independent vasodilator that induces relaxation via membrane hyperpolarization. The activation of IP3 receptors triggers the opening of potassium channels and hyperpolarization. Previously we found that relaxation to peroxynitrite was maintained during the development of atherosclerosis...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000461581
更新日期：2017-01-01 00:00:00
abstract：:Thrombospondin-1 (TSP1) is a potent natural inhibitor of angiogenesis. Although TSP1 has been reported to induce endothelial cell apoptosis in vitro and to downregulate neovascularization in vivo, the molecular mechanisms that link these two processes have yet to be established. Here we report that TSP1 mediates endot...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025733
更新日期：2000-05-01 00:00:00
abstract：:Most virus-infected cells release interferon-beta (IFN-beta) as a powerful inducer of antiviral defense. Endothelial cells tightly regulate local immune cell recruitment by expression of adhesion molecules and chemokines. Here, we studied the transcriptional regulation of IFN-beta-induced chemokine expression in prima...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000097977
更新日期：2007-01-01 00:00:00
abstract：:It has been well established that wall shear stress is an important determinant of endothelial cell function and gene expression as well as of its structure. There is increasing evidence that low wall shear stress, as present in artery bifurcations opposite to the flow divider where atherosclerotic lesions preferentia...
journal_title：Journal of vascular research
pub_type： 杂志文章,评审
doi：10.1159/000091648
更新日期：2006-01-01 00:00:00
abstract：:During the early stage of angiogenesis, neovascular sprouts are composed primarily of endothelial cells. As they mature, microvessels acquire a coating of mural cells, which are critical for the development and maintenance of a functional vasculature. Though growth factor regulation of mural cell recruitment has been ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000070711
更新日期：2003-03-01 00:00:00
abstract：:Intima-media thickening (IMT) of the carotid artery, a form of vascular remodeling, correlates well with coronary artery disease risk in humans. Vascular remodeling in response to blood flow is a complex process that critically involves altered cell matrix interactions. To gain insight into these events, we performed ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000081804
更新日期：2004-11-01 00:00:00
abstract：:Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo h...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000073902
更新日期：2003-09-01 00:00:00
abstract：:Previous studies have demonstrated endothelial and smooth muscle hyperplasia occur during arterial luminal expansion associated with elevation of arterial wall shear rates. The current study investigated whether remodeling induced by elevated wall shear would ultimately result in a vessel with intimal and medial cell ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025592
更新日期：1998-07-01 00:00:00
abstract：:The effect of insulin on the vasoconstriction induced by norepinephrine is presently controversial. Therefore, the aims of our study were: (1) to evaluate the effect of low- and high-dose insulin on the concentration-response curve to norepinephrine in small resistance arteries of spontaneously hypertensive rats (SHR)...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025679
更新日期：1999-09-01 00:00:00
abstract：:The aim of this study was to use periarterial manipulation to produce an atheroma-like neo-intima in rabbits and study resting blood flow and vascular responsiveness in vivo. One common carotid artery was enclosed in a silastic collar to induce a neo-intima similar to that of human early atherosclerosis, and carotid b...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159043
更新日期：1994-07-01 00:00:00
abstract：PURPOSE:To determine: (1) whether variables of a pulse wave form analysis of ocular microcirculation shown by laser speckle flowgraphy (LSFG) correlate with left ventricular (LV) systolic dysfunction and (2) whether these variables correlate with LV diastolic dysfunction in subjects without LV systolic dysfunction as a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000494066
更新日期：2018-01-01 00:00:00
abstract：:Endothelin-1 (ET-1), a vasoconstrictor peptide produced by endothelial and vascular smooth muscle cells (VSMC) might play a role in vascular remodelling. To investigate the proposed 'mitogenic' potential of ET-1, we examined the effects of chronic exposure of VSMC to ET-1 on cell cycle, growth/proliferation and differ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158994
更新日期：1993-07-01 00:00:00
abstract：:Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are cytoplasmic adaptor proteins of the TNF/interleukin (IL)-1/Toll-like receptor superfamily. Ligands of this family such as TNFα, CD40L, and IL-1β promote chronic inflammatory processes such as atherosclerosis and restenosis, the latter being a common a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000501615
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND/AIMS:Endoplasmic reticulum (ER) stress has emerged as a potential mechanism contributing to diabetes and its comorbidities. However, the importance of ER stress in diabetic vascular dysfunction is unclear. The purpose of this study was to examine the effects of the ER stress inhibitor, tauroursodeoxycholic a...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000479967
更新日期：2017-01-01 00:00:00
abstract：:The Cytoscan Model E-II (Cytometrics Inc., Philadelphia, Pa., USA) is a newly developed instrument which functions as an intravital microscope and is small and easily portable. Through the use of orthogonal polarization spectral (OPS) imaging, the Cytoscan Model E-II delivers images of the microcirculation which are c...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000054079
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND/AIMS:Dysfunction of endothelial progenitor cell (EPCs) contributes to diabetic vascular disease. We reported that downregulated miR-126 in diabetic patients causes EPC dysfunction. The study was designed to investigate how high glucose (HG) and advanced glycation end products (AGEs) regulate miR-126 expressi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000448713
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Transfer of molecular signals from veins to adjacent arteries is an established mechanism of vascular communication in the uterine circulation, which ultimately depends on venous permeability. This study tests the hypotheses that: (1) uterine veins are permeable to intravenous solutes, using a 3-kDa dextran ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000082976
更新日期：2005-01-01 00:00:00
abstract：:Organic nitrates act as vasodilators and have long been used for treatment of cardiovascular diseases. However, the therapeutic effect of nitrates is limited by induction of nitrate tolerance which is associated with endothelial dysfunction and enhanced vasoconstriction. Multiple mechanisms cause nitrate tolerance inc...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000065542
更新日期：2002-07-01 00:00:00
abstract：BACKGROUND:Extracellular matrix deposition is the main factor inducing stenotic lesions in arterial grafts. Lysyl oxidases (LOX) play a key role in stabilizing collagen and elastin. OBJECTIVE:To examine the repair response to arterial allografts in terms of LOX expression and collagen/elastin deposition using LOX inhi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000317399
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Infusion of exogenous vascular endothelial growth factor (VEGF) into adult brain at doses above 60 ng/day induces dramatic angiogenesis accompanied by vascular leak and inflammation. Blood vessels formed by this treatment are dilated and tortuous, exhibiting a pathological morphology. Pathological VEGF-induc...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000101450
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVES:Extracellular nucleotide release at the site of arterial injury mediates the proliferation and migration of vascular smooth muscle cells. Our aim was to investigate the role of the P2Y2 nucleotide receptor (P2Y2R) in neointimal hyperplasia. Approach and Results: Vascular injury was induced by the implantatio...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000449059
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Safety concerns associated with drug-eluting stents have spurred interest in alternative vessel therapeutics following angioplasty. Microbubble contrast agents have been shown to increase gene transfection in vivo in the presence of ultrasound. OBJECTIVES/METHODS:The purpose of this study was to determine w...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000258905
更新日期：2010-01-01 00:00:00
abstract：:Ruptured atherosclerotic plaques, lined with activated platelets, constitute an attractive target for magnetic resonance imaging (MRI). This study evaluated whether microparticles of iron oxide (MPIO) targeting ligand-induced binding sites (LIBS) on the activated conformation of glycoprotein IIb/IIIa could be used to ...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000135660
更新日期：2009-01-01 00:00:00
abstract：:The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A sp...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000025753
更新日期：2000-09-01 00:00:00
abstract：:There is evidence suggesting that fluid shear stress activates phospholipid turnover in endothelial cells, but it is not clear which phospholipids are involved in the transduction of the flow signal. Cultured human umbilical-vein endothelial cells were prelabeled with [14C]-arachidonic acid and subjected to laminar sh...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000158963
更新日期：1992-11-01 00:00:00
abstract：:Recent research in ultrasound technology has led to the development of a high-resolution echo-tracking device. The present study was performed to evaluate the accuracy in the measurement of human radial artery intima-media thickness with this new device. We determined the correlation between histological and ultrasoni...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000159037
更新日期：1994-03-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating peptide (PACAP; 1-38 and 1-27) and vasoactive intestinal peptide (VIP) are related neuropeptides of the secretin/glucagon family. Overlapping signaling through G-protein-coupled receptors mediates their vasomotor activity. We previously showed that PACAP deficiency (PACAP-KO) shi...
journal_title：Journal of vascular research
pub_type： 杂志文章
doi：10.1159/000481781
更新日期：2017-01-01 00:00:00
