Abstract：
:Fibroblast and epidermal growth factors (FGF-2 and EGF) are powerful mitogens for neural precursor cells isolated from the developing striatum and grown as neurospheres. However, questions remain as to the exact role of each of these molecules, and how the proteoglycan heparin may modify their behavior. Here, we show that FGF-2 is remarkably unstable in culture media, but that heparin could completely prevent its degradation, which led to faster cell growth rates. In addition, heparin significantly increased the number of cells within the E14 striatum responding to a brief pulse of FGF-2. In contrast, EGF was unable to stimulate the growth of E14 striatal precursors. However, EGF could induce the division of E18 striatal precursors as neurospheres and acted synergistically with FGF-2. FGF-2/heparin neurospheres generated significantly more neurons than EGF neurospheres. Interestingly, the addition of heparin to EGF neurospheres, which had no effects on EGF stability or growth rates, increased the numbers of neurons generated to that seen for FGF-2/heparin neurospheres. EGF neurospheres were found to produce FGF-2, but addition of heparin did not affect its concentration within cells or in the medium suggesting this released FGF-2 may already be bound to a proteoglycan. In addition, expanding cells with EGF plus heparin in the presence of an FGF-2 blocker did not have a significant effect on the number of neurons generated confirming that the increase in neuronal number is through a mechanism which is independent of FGF-2.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Caldwell MA,Garcion E,terBorg MG,He X,Svendsen CNdoi
10.1016/j.expneurol.2004.05.007keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
408-20issue
2eissn
0014-4886issn
1090-2430pii
S0014488604001797journal_volume
188pub_type
杂志文章abstract：:Oxaliplatin (OXA) is the common and extremely potent anti-advanced colorectal cancer chemotherapeutic. Accumulating evidence reveals that OXA evokes mechanical and cold hypersensitivity. However, the mechanism underlying these bothersome and dose-limiting adverse effects is poorly understood. It is well known that cyc...
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abstract：:Parkinson's disease (PD) is associated with complex compensatory changes in the functional and neurochemical anatomy of the basal ganglia resulting from striatal dopamine deficiency. Available evidence suggests that glutamatergic corticostriatal pathway becomes overactive following nigrostriatal dopaminergic denervati...
journal_title：Experimental neurology
pub_type： 杂志文章
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abstract：:The precise mechanisms underlying the efficacy of intravenous immunoglobulin (IVIg) in autoimmune neurological disorders including Guillain-Barré syndrome (GBS) are not known. Anti-ganglioside antibodies have been reported to be pathogenic in some variants of GBS, and we have developed passive transfer animal models t...
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abstract：:Increased intercellular coupling has been implicated in contributing to the synchronization of discharges characteristic of epileptic foci. Using RNA blot analysis, the level of mRNA for the heart-type gap junction protein, connexin43, is shown to be elevated in samples of temporal lobe neocortex obtained at the time ...
journal_title：Experimental neurology
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更新日期：1991-02-01 00:00:00
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journal_title：Experimental neurology
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journal_title：Experimental neurology
pub_type： 杂志文章
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2000.7496
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journal_title：Experimental neurology
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journal_title：Experimental neurology
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90226-2
更新日期：1985-02-01 00:00:00
abstract：:The extensive period of retinal ganglion cell (RGC) neurogenesis in the rat is associated with a protracted sequence of arrival of their axons into central targets such as the superior colliculus (SC) (Dallimore et al., 2002). Using in utero 5-bromo-2'-deoxyrudine (BrdU) injections to label early (embryonic day (E) 15...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2010.07.007
更新日期：2010-10-01 00:00:00
abstract：:Administration of endogenous corticosterone to intact animals induces calbindin-D28k protein in the hippocampal CA1-CA2 subfields. The fact that this effect on calbindin-D28k was shown to be specific for the hippocampus argues for a receptor-mediated effect on gene expression. In addition, chronic pretreatment with co...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6729
更新日期：1998-02-01 00:00:00
abstract：:Cinnamophilin (CINN, (8R, 8'S)-4, 4'-dihydroxy-3, 3'-dimethoxy-7-oxo-8, 8'-neolignan) protects against ischemic stroke in mice. While some anti-oxidative effects of CINN have been characterized, its therapeutic window and molecular basis for neuroprotection remain unclear. We evaluated antioxidant and anti-inflammator...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2009.01.019
更新日期：2009-05-01 00:00:00
abstract：:We assessed the potential of different MRI measures to detect and quantify skeletal muscle changes with denervation in two mouse models of denervation/neurogenic atrophy. Acute complete denervation and chronic partial denervation were examined in calf muscles after sciatic nerve axotomy and in transgenic SOD1(G93A) mi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.04.033
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abstract：:The precise role of hypothalamic norepinephrine (NE) in the control of vasopressin (AVP) release has remained unclear, due to reports of both inhibitory and excitatory effects of NE and only a few studies with direct hypothalamic manipulations. The present study utilized a chronically implanted swivel brain cannula to...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(90)90042-q
更新日期：1990-12-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2016.04.019
更新日期：2016-10-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(84)90084-0
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2005.07.019
更新日期：2005-11-01 00:00:00
abstract：:Many reports indicate that GM1 ganglioside is effective in reducing CNS ischemic injury in animal models. These models employ invasive surgery to induce ischemic damage in otherwise healthy animals. The purpose of this study was to determine if the beneficial effects of GM1 could be generalized to Spontaneously Hypert...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1994.1045
更新日期：1994-03-01 00:00:00
abstract：:Potassium and caffeine contractures of isolated small bundles (100 to 200 micron diameter) of muscle fibers isolated from the diaphragm of normal and dystrophic (C57BL/6J dy2J/dy2J) mice were compared. In diaphragms of pathologic mice (3 to 5 months old) the resting potential, the characteristics of the twitch, and so...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(84)90126-2
更新日期：1984-03-01 00:00:00
abstract：:The question of whether or not the development of dystrophic muscles is similar to that of normal muscles, prior to the manifestations of the symptoms of the disease, is investigated here. The developmental change in the activity of succinate dehydrogenase was therefore measured in individual fibers of prospectively d...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(87)90020-3
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90270-4
更新日期：1983-08-01 00:00:00
abstract：:Seizures were induced in immature 18-day-old rats by i.p. administration of homocysteine (11 mmol/kg) and the effects of selected antagonists of NMDA receptors [MK-801 (0.5 mg/kg), AP7 (0.33 mmol/kg), CGP 40116 (10 mg/kg)] and non-NMDA receptors [GDEE (4 mmol/kg), NBQX (two doses, 30 mg/kg each)] were studied. The eff...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6464
更新日期：1997-06-01 00:00:00
abstract：:Two neutral alpha-glucosidase isoenzymes were isolated from the muscle of Japanese quails with late-onset acid maltase deficiency. One isoenzyme is predominantly expressed in embryonic muscle and the other in adult muscle. The time of switching from one to the other of these two neutral alpha-glucosidases was the same...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90117-3
更新日期：1988-05-01 00:00:00
abstract：:Grafting fetal striatal cells into the brain of Huntington's disease (HD) patients has raised certain expectations in the past decade as an effective cell-based-therapy for this devastating condition. We argue that the first requirement for successful transplantation is defining the window of plasticity for the striat...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2014.04.021
更新日期：2014-07-01 00:00:00
abstract：:The role of interferon-gamma (IFN-gamma) in the pathogenesis of multiple sclerosis and experimental autoimmune encephalomyelitis (EAE) is still controversial. We have studied the function of IFN-gamma and its receptor in the EAE model using two different IFN-gamma receptor knockout (IFN-gamma R(-/-)) mouse types: C57B...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2001.7815
更新日期：2001-12-01 00:00:00
abstract：:Status epilepticus (SE) is an acute event, characterized by repeated or continuous seizures, which alters neuronal properties of the brain and can promote the epileptic disorder. Experimental observations indicate that SE becomes progressively less responsive to anti-epileptic drugs, suggesting changes in the underlyi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2012.01.020
更新日期：2012-04-01 00:00:00
