Abstract：
:The precise function of APP (Alzheimer's amyloid precursor protein) remains to be fully elucidated, but various lines of evidence suggest that it may be involved in cell adhesion processes. Because APP is a transmembrane glycoprotein, variations in its expression level may have direct bearing on its putative role in cell adhesion. Our results revealed that although APP levels did not change markedly with increasing cell density (ICD), there was a small but reproducible increase in APP expression at subconfluent conditions. Higher expression APP levels led to corresponding increases in the amount of APP processed and secreted APP (sAPP) released into the cell media. Given that phorbol esters stimulate the non-amyloidogenic pathway at the expense of reducing production of Abeta (the peptide found deposited as neuritic plaques in the brains of patients with Alzheimer's disease), thus providing an interesting therapeutic focus, we tested the effect of the phorbol 12-myristate 13-acetate (PMA) on APP processing at ICD. PMA not only stimulated sAPP release at all densities tested, but also produced a corresponding decrease in the intracellular levels of APP. Further experimentation revealed that increased APP expression with ICD was dependent on factors present in conditioned medium. Interestingly, exposing cells to the Abeta peptide itself could mimic these results, thus providing evidence for a potential positive feedback mechanism between Abeta production and intracellular APP levels.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Rebelo S,Henriques AG,da Cruz e Silva EF,da Cruz e Silva OAdoi
10.1002/jnr.20091keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
406-14issue
3eissn
0360-4012issn
1097-4547journal_volume
76pub_type
杂志文章abstract：:Living organotypic cocultures of rat thalamic and cortical explants were used to examine the effects of blocking action potential activity on the morphological development of axons in the mammalian neocortex. Studies in vivo have suggested that blocking sodium channel-dependent activity influences the growth character...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960315)43:6<707::AID-JNR
更新日期：1996-03-15 00:00:00
abstract：:The function of the prion protein is unknown despite suggestions it binds copper. Radioactive copper (Cu(67)) was used to demonstrate that histidine-dependent uptake of copper by cerebellar cells in culture is related to the level of PrP(c) expression. Copper is released by neurones at the synapse. Veratridine-induced...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:The ED1 monoclonal antibody recognizes an antigen in lysosomal membranes of phagocytes. The expression of this antigen in cells increases during phagocytic activity. Here we describe the expression of ED1-immunoreactivity during the various stages of both acute (monophasic) and chronic relapsing experimental autoimmun...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490380402
更新日期：1994-07-01 00:00:00
abstract：:This study demonstrates the ability of proteasome inhibitors (lactacystin, MG 115, MG 132) adenosine diphosphate to induce a time- and dose-dependent increase in poly-ADP-ribosylation (PAR) in the neural PC6 cell line, a subclone of PC12 cells. Elevated levels of PAR contribute to the toxicity associated with impaired...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20000815)61:4<436::AID-JNR10>3.0
更新日期：2000-08-15 00:00:00
abstract：:The rate and direction of neurite growth have been shown in a number of studies to be determined by the distribution of adhesive sites on the growth cone. Recent evidence showing that the application of extrinsic electric fields can redistribute membrane molecules and alter both the rate and direction of neurite growt...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130118
更新日期：1985-01-01 00:00:00
abstract：:Cultures of rat C6 rat glioma cells exhibit a diminished response to isoproterenol and forskolin after being treated with phorbol 12,13-dibutyrate (PDbU). An IC50 for PDbU of 38 +/- 5 nM and 62 +/- 8 nM was observed in the isoproterenol and forskolin response, respectively. Similarly, C6 cultures exhibited a diminishe...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250110
更新日期：1990-01-01 00:00:00
abstract：:Astrocytes are excitable neural cells that contribute to brain information processing via bidirectional communication with neurons. This involves the release of gliosignaling molecules that affect synapses patterning and activity. Mechanisms mediating the release of these molecules likely consist of non-vesicular and ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24051
更新日期：2017-11-01 00:00:00
abstract：:Grafting fetal ventral mesencephalon has been utilized to alleviate the symptoms of Parkinson's disease. One obstacle in using this approach is the limited outgrowth from the transplanted dopamine neurons. Thus, it is important to evaluate factors that promote outgrowth from fetal dopamine neurons. Proteoglycans (PGs)...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21465
更新日期：2008-01-01 00:00:00
abstract：:Although proteins belonging to the sHSP superfamily are diverse in sequence and size, most share characteristic features, including 1) a small molecular mass of 12-43 kDa, 2) a conserved alpha-crystallin domain of 80-100 residues, 3) formation of large oligomers, 4) a dynamic quaternary structure, and 5) induction by ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.21682
更新日期：2008-08-01 00:00:00
abstract：:Axotomy of peripheral nerves in neonatal rats induces motoneuron death that can be delayed but not arrested by the application of several neurotrophic factors (NFs) or adenoviral vectors carrying genes for NFs. We tested whether an adenoviral vector carrying the gene for glial cell-line-derived neurotrophic factor (Ad...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981215)54:6<766::AID-JNR
更新日期：1998-12-15 00:00:00
abstract：:We have demonstrated recently that atypical antipsychotics possess neuroprotective actions in H2O2-mediated and serum-withdrawal models of cell death. In the present study, we compared the ability of atypical and typical antipsychotics to protect against an insult mediated by Abeta(25-35), an apoptogenic fragment of t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10832
更新日期：2003-12-15 00:00:00
abstract：:Hyperglycemia and acidosis are the key factors in diabetic complications. It has been shown that acute or chronic diabetes alters serotonin levels in brain. However, the mechanism of hyperglycemia- or acidosis-induced changes in serotonin levels remains poorly understood. Because Ca2+-dependent protein kinases play a ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20499
更新日期：2005-05-15 00:00:00
abstract：:The ability of altered environmental conditions to modulate some properties of synaptosomes has been studied. Incubation conditions used included the presence of methyl mercury or an organochlorine insecticide: chlordecone. Other adverse chemical conditions during incubation were the absence of calcium salts from the ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250211
更新日期：1990-02-01 00:00:00
abstract：:In the present study, following previous experience with electrolytic lesion of the rat brain, and subsequent reduction of reactive gliosis with 7 beta-hydroxycholesterol derivatives (Bochelen et al.: Neuroscience 51:827-834, 1992), we have performed a hemisection of the spinal cord in adult rats and investigated the ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490410110
更新日期：1995-05-01 00:00:00
abstract：:The Shaw subfamily of potassium channel genes, including Kv3.1, are highly expressed within the auditory nuclei of the brainstem, where they have been implicated in the characteristic response properties of particular types of neurons. Potassium currents carried by Kv3.1 are voltage-dependent, have a high activation t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19991215)58:6<791::aid-jnr
更新日期：1999-12-15 00:00:00
abstract：:The thrombospondin (TSP) family of extracellular matrix glycoproteins are widely expressed in the developing and adult central nervous system although their function remains poorly defined. We have used cell culture techniques to analyse the expression and function of TSPs in glial cells derived from myelinated region...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19971015)50:2<202::AID-JNR
更新日期：1997-10-15 00:00:00
abstract：:Nerve growth factor (NGF) is a protein essential for the survival and normal function of sympathetic neurons. Two-site immunoassays have been developed over the past decade in several laboratories and used to estimate its endogenous concentrations in a variety of effector tissues. However, levels appear restricted to ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19961201)46:5<581::AID-JNR
更新日期：1996-12-01 00:00:00
abstract：:Structural microtubule-associated proteins (MAPs) interact with microtubules to regulate the various dynamic stages of microtubules. The purpose of this study was to measure the impact of myc-tagged MAP1a fragments on microtubule dynamic phases in vivo. Cells from an epithelial kidney cell line (LLCPK1) that had been ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21920
更新日期：2009-04-01 00:00:00
abstract：:To further our understanding of the functions of the major myelin proteins, myelin basic protein (MBP) and proteolipid protein (PLP), and other myelin proteins, such as 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and myelin-associated glycoprotein (MAG), bovine brain myelin was extracted with Triton X-100, and p...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10383
更新日期：2002-10-01 00:00:00
abstract：:Mg2+ATPase and (Na+ + K+)ATPase activities were measured in clonal line NN hamster astroblasts and in clonal lines M1 and N1E-115 mouse neuroblastoma cells after the cells had been subjected to the acute and chronic actions of 100 mM ethanol. Exposure of the astroblasts to ethanol for periods as long as 68 days produc...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490020205
更新日期：1976-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200306
更新日期：1988-07-01 00:00:00
abstract：:Transferrin is the second most abundant plasma protein and functions to transport iron. It is an essential constituent in culture media for virtually all cells. In a recent study, we reported that transferrin (Tf) is specifically located in oligodendrocytes in the rat nervous system. This investigation examines immuno...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490170108
更新日期：1987-01-01 00:00:00
abstract：:Niemann-Pick C (NPC) disease is an autosomal recessive lipidosis characterized by lysosomal accumulations of unesterified cholesterol. Cultured NPC cells exhibit a defect in the intracellular trafficking of LDL-derived cholesterol that leads to lysosomal accumulations of unesterified cholesterol. We found in a prelimi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr
更新日期：1997-08-01 00:00:00
abstract：:The role of the plasmin-generating system, a serum component, in the development of dissociated embryonic chick spinal cord cells in culture was studied. Studies were performed in a defined system where the cells were maintained in a serum-free medium. Under these conditions the cells produce plasminogen activator. It...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490080237
更新日期：1982-01-01 00:00:00
abstract：:This paper describes a novel technique to identify and study neurons that express specific mRNAs. The method is called Live FISH because it is like fluorescent in situ hybridization (FISH) but can be applied to living tissues. In Live FISH, molecular beacons, which fluoresce only in the presence of the complementary m...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22191
更新日期：2010-01-01 00:00:00
abstract：:Mitochondrial dysfunction has been implicated in cell death in many neurodegenerative diseases. Diminished activity of the alpha-ketoglutarate dehydrogenase complex (KGDHC), a key and arguably rate-limiting enzyme of the Krebs cycle, occurs in these disorders and may underlie decreased brain metabolism. The present st...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10756
更新日期：2003-10-15 00:00:00
abstract：:Thirty-day readmission rates has been increasingly used by clinicians, hospital administrators, and policy makers as a metric for the quality of care. However, the 30-day readmission rates may be affected by other factors intrinsic to the patient and may not be a good measure of the quality of care provided by the hos...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24398
更新日期：2020-01-01 00:00:00
abstract：:Fatty acid amide hydrolase (FAAH) is a membrane-bound enzyme activity that degrades neuromodulatory fatty acid amides, including oleamide and anandamide. A single 2.5-kb FAAH mRNA is distributed throughout the rat CNS and accumulates progressively between embryonic day 14 and postnatal day 10, remains high until postn...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19971215)50:6<1047::AID-JN
更新日期：1997-12-15 00:00:00
abstract：:Fast oscillations at approximately 200 Hz, termed ripples, occur in the hippocampus and cortex of several species, including humans, and are thought to play a role in physiological (e.g., sensory information processing or memory consolidation) and pathological (e.g., seizures) processes. Blocking gamma-aminobutyric ac...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20440
更新日期：2005-04-01 00:00:00
abstract：:Cranial idiopathic acute peripheral facial nerve paralysis (Bell palsy) has been used as a model disease in the present study mainly to investigate trophic capacity of serum components. The ability to induce sprouting from chick embryo dorsal root ganglia was used as the bioassay for trophic activity. Sera from normal...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490090411
更新日期：1983-01-01 00:00:00
