Abstract:
:Entry into mitosis is driven by signaling cascades of mitotic kinases. Our recent studies show that TTK, a kinetochore-associated protein kinase, interacts with CENP-E, a mitotic kinesin located to corona fiber of kinetochore. Using immunoelectron microscopy, here we show that TTK is present at the nuclear pore adjacent complex of interphase HeLa cells. Upon nuclear envelope fragmentation, TTK targets to the outermost region of the developing kinetochores of monoorient chromosome as well as to spindle poles. After stable attachment, throughout chromosome congression, TTK is a constituent of the corona fibers, extending up to 90 nm away from the kinetochore outer plate. Upon metaphase alignment, TTK departs from the kinetochore and migrates toward the centrosomes. Taken together, this evidence strongly supports a model in which TTK functions in spindle checkpoint signaling cascades at both kinetochore and centrosome.
journal_name
Cell Resjournal_title
Cell researchauthors
Dou Z,Sawagechi A,Zhang J,Luo H,Brako L,Yao XBdoi
10.1038/sj.cr.7290186keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
443-9issue
6eissn
1001-0602issn
1748-7838journal_volume
13pub_type
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