Abstract:
:Amycolatopsis mediterranei is used for industry-scale production of rifamycin, which plays a vital role in antimycobacterial therapy. As the first sequenced genome of the genus Amycolatopsis, the chromosome of strain U32 comprising 10,236,715 base pairs, is one of the largest prokaryotic genomes ever sequenced so far. Unlike the linear topology found in streptomycetes, this chromosome is circular, particularly similar to that of Saccharopolyspora erythraea and Nocardia farcinica, representing their close relationship in phylogeny and taxonomy. Although the predicted 9,228 protein-coding genes in the A. mediterranei genome shared the greatest number of orthologs with those of S. erythraea, it was unexpectedly followed by Streptomyces coelicolor rather than N. farcinica, indicating the distinct metabolic characteristics evolved via adaptation to diverse ecological niches. Besides a core region analogous to that common in streptomycetes, a novel 'quasi-core' with typical core characteristics is defined within the non-core region, where 21 out of the total 26 gene clusters for secondary metabolite production are located. The rifamycin biosynthesis gene cluster located in the core encodes a cytochrome P450 enzyme essential for the conversion of rifamycin SV to B, revealed by comparing to the highly homologous cluster of the rifamycin B-producing strain S699 and further confirmed by genetic complementation. The genomic information of A. mediterranei demonstrates a metabolic network orchestrated not only for extensive utilization of various carbon sources and inorganic nitrogen compounds but also for effective funneling of metabolic intermediates into the secondary antibiotic synthesis process under the control of a seemingly complex regulatory mechanism.
journal_name
Cell Resjournal_title
Cell researchauthors
Zhao W,Zhong Y,Yuan H,Wang J,Zheng H,Wang Y,Cen X,Xu F,Bai J,Han X,Lu G,Zhu Y,Shao Z,Yan H,Li C,Peng N,Zhang Z,Zhang Y,Lin W,Fan Y,Qin Z,Hu Y,Zhu B,Wang S,Ding X,Zhao GPdoi
10.1038/cr.2010.87subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1096-108issue
10eissn
1001-0602issn
1748-7838pii
cr201087journal_volume
20pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract::Although VEGFR-3 deficiency disrupts blood vascular development during early embryogenesis, the underlying mechanism was not clear. To characterize its function in angiogenesis and lymphangiogenesis, we employed two genetically modified mouse models in this study, targeting the coding region for the ligand-binding dom...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2010.116
更新日期:2010-12-01 00:00:00
abstract::A monoclonal antibody, LC-1, recognizing lung cancer associated common antigens was obtained in authors' laboratory. Its single chain Fv fragment (ScFv) named LC-1 ScFv was constructed based on recombinant phage displayed techniques. For expression on cell membrane, LC-1 ScFv was cloned into pDisplay vector, which dir...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290109
更新日期:2002-03-01 00:00:00
abstract::This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for beta-glucuroni...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290092
更新日期:2001-09-01 00:00:00
abstract::Previously, mouse bone marrow-derived stem cells (MSC) treated with the unspecific DNA methyltransferase inhibitor 5-azacytidine were reported to differentiate into cardiomyocytes. The aim of the present study was to investigate the efficiency of a similar differentiation strategy in human mononuclear cells obtained f...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310116
更新日期:2006-12-01 00:00:00
abstract::NF-κBs are a family of transcription factors that control a number of essential cellular functions including immune responses, cell proliferation and antiapoptosis. NF-κB activities are tightly regulated through upstream signaling molecules and downstream feedback loops. In this review, structural discoveries in the N...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2010.171
更新日期:2011-01-01 00:00:00
abstract::Our previous study showed that hydroxyurea (Hu) could induce HEL cells to express human beta-globin gene. However the molecular mechanisms by which the expression of beta-globin gene is activated and regulated are poorly understood. Here we show that the binding patterns between the core DNA sequences (HS2 core sequen...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290144
更新日期:2002-12-01 00:00:00
abstract::Brain tumors are dynamic complex ecosystems with multiple cell types. To model the brain tumor microenvironment in a reproducible and scalable system, we developed a rapid three-dimensional (3D) bioprinting method to construct clinically relevant biomimetic tissue models. In recurrent glioblastoma, macrophages/microgl...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-020-0338-1
更新日期:2020-10-01 00:00:00
abstract::Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial c...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7310039
更新日期:2006-03-01 00:00:00
abstract::The endoplasmic reticulum (ER) is composed of the nuclear envelope, perinuclear sheets and a peripheral tubular network. The peripheral ER and mitochondria form tight contacts at specific subdomains, which coordinate the functions of the two organelles and are required for multiple cellular processes such as Ca2+ tran...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-018-0065-z
更新日期:2018-08-01 00:00:00
abstract::Dysregulation of beta-site APP-cleaving enzyme (BACE) and/or gamma-secretase leads to anomalous production of amyloid-beta peptide (Abeta) and contributes to the etiology of Alzheimer's disease (AD). Since these secretases mediate proteolytic processing of numerous proteins, little success has been achieved to treat A...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2010.3
更新日期:2010-02-01 00:00:00
abstract::Recent outbreaks of Zika virus (ZIKV) highlight an urgent need for therapeutics. The protease complex NS2B-NS3 plays essential roles during flaviviral polyprotein processing, and thus represents an attractive drug target. Here, we developed a split luciferase complementation-based high-throughput screening assay to id...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2017.88
更新日期:2017-08-01 00:00:00
abstract::Spontaneous Ca2+ oscillations in vascular smooth muscle cells have been modeled using a single Ca2+ pool. This report describes spontaneous Ca2+ oscillations dependent on two separate Ca2+ sources for the nuclear versus cytoplasmic compartments. Changes in free intracellular Ca2+ were monitored with ratiometric Ca2+- ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290238
更新日期:2004-10-01 00:00:00
abstract::The Wnt/β-catenin pathway plays important roles in the differentiation of multiple cell types, including mesenchymal stem cells. Using a cell-based chemical screening assay with a synthetic chemical library of 270 000 compounds, we identified the compound SKL2001 as a novel agonist of the Wnt/β-catenin pathway and unc...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.127
更新日期:2012-01-01 00:00:00
abstract::RIG-I belongs to a type of intracellular pattern recognition receptors involved in the recognition of viral RNA by the innate immune system. A report by Peisley et al. published in Nature provides the crystal structure of human RIG-I revealing a tetrameric architecture of the RIG-I 2-CARD domain bound by three K63-lin...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2014.58
更新日期:2014-06-01 00:00:00
abstract::Parthenogenetic embryonic stem (pES) cells provide a valuable in vitro model system for studying the molecular mechanisms that underlie genomic imprinting. However, the pluripotency of pES cells and the expression profiles of paternally expressed imprinted genes have not been fully explored. In this study, three mouse...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2007.70
更新日期:2007-09-01 00:00:00
abstract::In mice treated with antibiotics to deplete commensal microbiota, there is a significant overhaul of host cellular disposition and function with CX3CR1+ mononuclear phagocytic cells carrying pathogenic and non-pathogenic administered bacteria to the (messenteric lymph node) MLN, resulting in T cell stimulation and IgA...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2013.41
更新日期:2013-05-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Cell research
pub_type: 已发布勘误
doi:10.1038/s41422-019-0262-4
更新日期:2020-02-01 00:00:00
abstract::Macrophages are important innate immune cells with functions in tissue repair and remodeling, induction and resolution of inflammation, as well as elimination of invading pathogens. In a recent study, Schmidt and colleagues describe the open epigenetic landscape of the human inflammatory macrophages, and the transcrip...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2016.5
更新日期:2016-02-01 00:00:00
abstract::DNA double-strand breaks (DSBs) are introduced in cells by ionizing radiation and reactive oxygen species. In addition, they are commonly generated during V(D)J recombination, an essential aspect of the developing immune system. Failure to effectively repair these DSBs can result in chromosome breakage, cell death, on...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2008.3
更新日期:2008-01-01 00:00:00
abstract::CRISPR-Cas9 and CRISPR-Cpf1 systems have been successfully harnessed for genome editing. In the CRISPR-Cas9 system, the preordered A-form RNA seed sequence and preformed protein PAM-interacting cleft are essential for Cas9 to form a DNA recognition-competent structure. Whether the CRISPR-Cpf1 system employs a similar ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2016.88
更新日期:2016-08-01 00:00:00
abstract::A recent study led by Professor Rob Martienssen in Cell showed that 3'-tRNA-derived small RNAs can suppress long terminal repeat retrotransposon activity in mammalian cells by mechanisms independent of DNA-associated epigenetic marks, suggesting how the genome may defend itself from retrotransposon invasion during epi...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2017.109
更新日期:2017-11-01 00:00:00
abstract::The completion of genome sequences and subsequent high-throughput mapping of molecular networks have allowed us to study biology from the network perspective. Experimental, statistical and mathematical modeling approaches have been employed to study the structure, function and dynamics of molecular networks, and begin...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/cr.2008.16
更新日期:2008-02-01 00:00:00
abstract::Intracellular redox homeostasis plays a critical role in determining tumor cells' sensitivity to drug-induced apoptosis. Here we investigated the role of thioredoxin-1 (TRX1), a key component of redox regulation, in arsenic trioxide (As(2)O(3))-induced apoptosis. Over-expression of wild-type TRX1 in HepG(2) cells led ...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2007.112
更新日期:2008-04-01 00:00:00
abstract::Plant reproduction is initiated by the specification of sporocytes that form haploid spores through meiosis. A new study in Arabidopsis published in Cell Research shows how the product of sporocyteless/nozzle, a key gene in this process, partners with co-repressors and transcription factors to promote spore formation,...
journal_title:Cell research
pub_type: 评论,杂志文章
doi:10.1038/cr.2014.164
更新日期:2015-01-01 00:00:00
abstract::Transforming growth factor beta (TGF-beta) carries out tumor suppressor activity in epithelial and lymphoid cells, whereas telomerase is required for most cancers. Although the molecular mechanisms by which TGF-beta acts as a tumor suppressor are yet to be fully established, a link between TGFb and its tumor suppresso...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7310023
更新日期:2006-02-01 00:00:00
abstract::Various factors/pathways including hormonal regulation have been suggested to control herpes simplex virus type 1 (HSV-1) latency and reactivation. Our computer analysis identified a DNA repeat containing thyroid hormone-responsive elements (TRE) in the regulatory region of HSV-1 latency-associated transcript (LAT). T...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2010.50
更新日期:2010-05-01 00:00:00
abstract::Autophagy is a conserved process that delivers cytosolic substances to the lysosome for degradation, but its direct role in the regulation of antiviral innate immunity remains poorly understood. Here, through high-throughput screening, we discovered that CCDC50 functions as a previously unknown autophagy receptor that...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/s41422-020-0362-1
更新日期:2021-01-01 00:00:00
abstract::Apoptosis is a complex process involving a large array of genes and mutation of any of these genes may lead to malignancy formation. Re-acquirement of FasL by tumor cells may enable them to evade the surveillance of immune system and thus contributes to the growth of tumor. Apart from traditional therapies, inducing a...
journal_title:Cell research
pub_type: 杂志文章,评审
doi:10.1038/sj.cr.7290060
更新日期:2001-03-01 00:00:00
abstract::In mammals, the inheritance of mitochondrion and its DNA (mtDNA) is strictly maternal, despite the fact that a sperm can inject up to 100 functional mitochondria into the oocyte during fertilization. The mechanisms responsible for the elimination of the paternal mitochondria remain largely unknown. We report here that...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/cr.2011.182
更新日期:2011-12-01 00:00:00
abstract::Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have been considered for an...
journal_title:Cell research
pub_type: 杂志文章
doi:10.1038/sj.cr.7290275
更新日期:2005-02-01 00:00:00