Abstract:
:We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which there is a deficit of 60S ribosomal subunits. Cold sensitivity and the assembly defect are recessive and cosegregate, defining a single essential gene that we designated DRS1 (deficiency of ribosomal subunits). The wild-type DRS1 gene was cloned by complementation of the cold-sensitive phenotype of drs1. Sequence analysis reveals a high degree of similarity to a family of proteins that are thought to function as ATP-dependent RNA helicases. Pulse-chase analysis of ribosomal RNA synthesis and processing indicates that the drs1 mutant accumulates the 27S precursor of the mature 25S rRNA. These results suggest that, as in pre-mRNA splicing, RNA helicase activities are involved in ribosomal RNA processing.
journal_name
Proc Natl Acad Sci U S Aauthors
Ripmaster TL,Vaughn GP,Woolford JL Jrdoi
10.1073/pnas.89.23.11131keywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
11131-5issue
23eissn
0027-8424issn
1091-6490journal_volume
89pub_type
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