Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus.

abstract：:The corticotropin-releasing factor (CRF) ligand family has diverse effects on the CNS, including the modulation of the stress response. The ligands' effects are mediated by binding to CRF G protein-coupled receptors. We have determined the 3D NMR structure of the N-terminal extracellular domain (ECD1) of the mouse CRF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grace CR,Perrin MH,DiGruccio MR,Miller CL,Rivier JE,Vale WW,Riek R

更新日期：2004-08-31 00:00:00

abstract：:A nuclear protein, termed leucine-rich acidic nuclear protein (LANP), has been isolated from among rat cerebellar proteins whose expression was transiently increased during an early stage of postnatal development. The amino acid sequence, deduced from its cDNA, showed that LANP contains 247 amino acids consisting of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuoka K,Taoka M,Satozawa N,Nakayama H,Ichimura T,Takahashi N,Yamakuni T,Song SY,Isobe T

更新日期：1994-10-11 00:00:00

abstract：:Estimation of gene diversity (heterozygosity) at 1442 single-nucleotide polymorphism (SNP) loci in an ethnically diverse sample of humans revealed consistently reduced gene diversities at SNP loci causing amino acid changes, particularly those causing amino acid changes predicted to be disruptive to protein structure....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes AL,Packer B,Welch R,Bergen AW,Chanock SJ,Yeager M

更新日期：2003-12-23 00:00:00

abstract：:To optimize the use of modified adenoviruses as vectors for gene delivery to the myocardium, we have characterized infection of cultured fetal and adult rat cardiac myocytes in vitro and of adult cardiac myocytes in vivo by using a replication-defective adenovirus carrying the chloramphenicol acetyltransferase (CAT) r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kass-Eisler A,Falck-Pedersen E,Alvira M,Rivera J,Buttrick PM,Wittenberg BA,Cipriani L,Leinwand LA

更新日期：1993-12-15 00:00:00

abstract：:We have developed a procedure for purifying highly specific polyclonal antibodies against 5-methylcytosine. These antibodies were used to probe the distribution of 5-methylcytosine among fractionated nucleosomes of mouse cell chromatin. Our results demonstrate that at least 80% of the 5-methylcytosine is localized in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball DJ,Gross DS,Garrard WT

更新日期：1983-09-01 00:00:00

abstract：:Granulocyte-colony stimulating factor (G-CSF) induces differentiation of M1 murine myeloid leukemia cells into mature granulocytes/macrophages and also causes accumulation of the cells in the G1 phase of the cell cycle. We examined, therefore, whether synchronization of M1 cells in the G1 phase could affect G-CSF-indu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsuda H,Neckers LM,Pluznik DH

更新日期：1986-06-01 00:00:00

abstract：:Charge transfer at metallo-molecular interfaces may be used to design multifunctional hybrids with an emergent magnetization that may offer an eco-friendly and tunable alternative to conventional magnets and devices. Here, we investigate the origin of the magnetism arising at these interfaces by using different techni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Al Ma'Mari F,Rogers M,Alghamdi S,Moorsom T,Lee S,Prokscha T,Luetkens H,Valvidares M,Teobaldi G,Flokstra M,Stewart R,Gargiani P,Ali M,Burnell G,Hickey BJ,Cespedes O

更新日期：2017-05-30 00:00:00

abstract：:Surfaces with patterned wettability contrast are important in industrial applications such as heat transfer, water collection, and particle separation. Traditional methods of fabricating such surfaces rely on microfabrication technologies, which are only applicable to certain substrates and are difficult to scale up a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen D,McKinley GH,Cohen RE

更新日期：2016-07-19 00:00:00

abstract：:High sensitivity differential scanning calorimetry is applied to the study of the thermotropic behavior of mixtures of synthetic phospholipids in multilamellar aqueous suspensions. The systems dimyristoylphosphatidylcholine dipalmitoylphosphatidylcholine, and dimyristoylphosphatidylethanolamine-distearoylphosphatidyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mabrey S,Sturtevant JM

更新日期：1976-11-01 00:00:00

abstract：:Tropical deforestation for the establishment of tree cash crop plantations causes significant alterations to soil organic carbon (SOC) dynamics. Despite this recognition, the current Intergovernmental Panel on Climate Change (IPCC) tier 1 method has a SOC change factor of 1 (no SOC loss) for conversion of forests to p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Straaten O,Corre MD,Wolf K,Tchienkoua M,Cuellar E,Matthews RB,Veldkamp E

更新日期：2015-08-11 00:00:00

abstract：:Chronic exposure of various cell types to adrenergic agonists leads to a decrease in cell surface beta 2-adrenergic receptor (beta 2AR) number. Sequestration of the receptor away from the cell surface as well as a down-regulation of the total number of cellular receptors are believed to contribute to this agonist-medi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valiquette M,Bonin H,Hnatowich M,Caron MG,Lefkowitz RJ,Bouvier M

更新日期：1990-07-01 00:00:00

abstract：:It is demonstrated that, properly represented, the amino acid composition of protein sequences contains the information necessary to delineate the global properties of protein structure space. A numerical representation of amino acid sequence in terms of a set of property factors is used, and the values of those prope...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S

更新日期：2009-08-25 00:00:00

abstract：:It has been proposed that the causative agent of scrapie represents a class of infectious particle that is devoid of nucleic acid and that an altered form of the endogenous prion protein (PrP) is the agent. However, it has been difficult to exclude the possibility that PrP purified from scrapie tissues might be contam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caughey B,Race RE,Vogel M,Buchmeier MJ,Chesebro B

更新日期：1988-07-01 00:00:00

abstract：:A new class of Escherichia coli mutants, referred to as grn, has been isolated by localized mutagenesis. These mutations affect the sigma subunit of DNA-dependent RNA polymerase (ribonucleoside 5'-triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) by abolishing the expression of the lambda N gene, and they are close...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,Kurihara T,Saito H,Uchida H

更新日期：1979-09-01 00:00:00

abstract：:Isolation and chromatography of collagen from chick cartilage revealed an excess of alpha1 relative to alpha2-chains. Characterization of the cyanogen bromide peptides from the alpha1 fraction indicated the presence of two homologous forms of alpha1. The results suggest that cartilage contains two collagens, one ident...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller EJ,Matukas VJ

更新日期：1969-12-01 00:00:00

abstract：:IL-17A is a therapeutic target in many autoimmune diseases. Most nonhematopoietic cells express IL-17A receptors and respond to extracellular IL-17A by inducing proinflammatory cytokines. The IL-17A signal transduction triggers two broad, TRAF6- and TRAF5-dependent, intracellular signaling pathways to produce represen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McGowan J,Peter C,Kim J,Popli S,Veerman B,Saul-McBeth J,Conti H,Pruett-Miller SM,Chattopadhyay S,Chakravarti R

更新日期：2020-10-06 00:00:00

abstract：:Membrane-associated and periplasmic proteins of Agrobacterium tumefaciens C-58 were compared with those from avirulent (nontumrigenic) derivative strains by slab and two-dimensional gel electrophoresis. Two proteins (Per-I and Per-2), with a molecular weight of 37,500 and 37,300, respectively, were detected in the sup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sonoki S,Kado CI

更新日期：1978-08-01 00:00:00

abstract：:Neuropeptide B (NPB) and neuropeptide W (NPW) have been recently identified as ligands for the G protein-coupled receptor (GPR) 7 and GPR8. The precise in vivo role of this neuropeptide-receptor pathway has not been fully demonstrated. In this paper, we report that NPB-deficient mice manifest a mild adult-onset obesit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly MA,Beuckmann CT,Williams SC,Sinton CM,Motoike T,Richardson JA,Hammer RE,Garry MG,Yanagisawa M

更新日期：2005-07-12 00:00:00

abstract：:A closed duplex DNA molecule relaxed and containing nucleosomes has a different linking number from the same molecule relaxed and without nucleosomes. What does this say about the structure of the nucleosome? A mathematical study of this question is made, representing the DNA molecule by a ribbon. It is shown that the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuller FB

更新日期：1978-08-01 00:00:00

abstract：:p19ARF has been implicated as a key regulator of p53 stability and activation. While numerous stresses activate the p53 growth arrest pathway, those requiring p19ARF remain to be elucidated. We used p19ARF knockout mouse embryo fibroblasts to show that DNA damage and microtubule disruption require p19ARF to induce p53...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan SH,Moritsugu J,Wahl GM

更新日期：2000-03-28 00:00:00

abstract：:Cyclic nucleotide metabolism was investigated in growing kidneys of rats during compensatory hypertrophy and during neonatal development. After unilateral nephrectomy a mild and short-lasting decrease in cyclic 3':5" adenosine monophosphate (cAMP) was observed in the hypertrophying kidney. In contrast, cyclic 3':5' gu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schlondorff D,Weber H

更新日期：1976-02-01 00:00:00

abstract：:We image macroscopic transient pores in mechanically stretched giant vesicles. Holes open above a critical radius r(c1), grow up to a radius r(c2), and close. We interpret the upper limit r(c2) by a relaxation of the membrane tension as the holes expand. The closing of the holes is caused by a further relaxation of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandre O,Moreaux L,Brochard-Wyart F

更新日期：1999-09-14 00:00:00

abstract：:Tobacco mosaic virus (TMV) RNA was encapsulated in large, unilamellar phospholipid vesicles (liposomes), and the encapsulated TMV RNA was shown to be infectious when incubated with tobacco protoplasts under appropriate conditions. Maximal virus production in protoplasts was observed after their incubation with TMV RNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fraley RT,Dellaporta SL,Papahadjopoulos D

更新日期：1982-03-01 00:00:00

abstract：:Triglyceridemic response to a standard oral fat meal was determined in 28 healthy subjects and related to the levels of several lipids, lipoproteins, and apolipoproteins in the post-absorptive plasma. A fatty test meal was administered orally, and postprandial plasma triglyceride levels were determined. In the fasting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patsch JR,Karlin JB,Scott LW,Smith LC,Gotto AM Jr

更新日期：1983-03-01 00:00:00

abstract：:Genes carried by EcoRI endonuclease-generated fragments of Staphylococcus plasmid DNA have been covalently joined to the E. coli antibiotic-resistance plasmid pSC101, and the resulting hybrid molecules have been introduced into E. coli by transformation. The newly constructed plasmids replicate as biologically functio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang AC,Cohen SN

更新日期：1974-04-01 00:00:00

abstract：:The two internal transcribed spacers (ITS1 and ITS2) of rDNA of three members of the Ixodes ricinus "complex" (Acari: Ixodidae) were sequenced. Sequence variation was assessed for the North American species I. scapularis, I. dammini, and I. pacificus at three levels: within individual/population, between individuals o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wesson DM,McLain DK,Oliver JH,Piesman J,Collins FH

更新日期：1993-11-01 00:00:00

abstract：:After exposure of segments of rabbit taenia coli to the nonionic detergent Triton X-100, tension could be induced by increasing the [Ca2+] in the micromolar range. In the presence of a saturating [Ca2+], this preparation developed nearly 100% of the control tetanus tension recorded from the intact muscle prior to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon AR

更新日期：1978-07-01 00:00:00

abstract：:The formation of myelin by Schwann cells (SCs) occurs via a series of orchestrated molecular events. We previously used global expression profiling to examine peripheral nerve myelination and identified the NAD(+)-dependent deacetylase Sir-two-homolog 2 (Sirt2) as a protein likely to be involved in myelination. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beirowski B,Gustin J,Armour SM,Yamamoto H,Viader A,North BJ,Michán S,Baloh RH,Golden JP,Schmidt RE,Sinclair DA,Auwerx J,Milbrandt J

更新日期：2011-10-25 00:00:00

abstract：:Non-human primates (NHP) provide crucial research models. Their strong similarities to humans make them particularly valuable for understanding complex behavioral traits and brain structure and function. We report here the genetic mapping of an NHP nervous system biologic trait, the cerebrospinal fluid (CSF) concentra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freimer NB,Service SK,Ophoff RA,Jasinska AJ,McKee K,Villeneuve A,Belisle A,Bailey JN,Breidenthal SE,Jorgensen MJ,Mann JJ,Cantor RM,Dewar K,Fairbanks LA

更新日期：2007-10-02 00:00:00

abstract：:A biomimetic hydrogel platform was designed to signal encapsulated cells using immobilized cell-cell communication cues, with a focus on enhancing the survival and function of encapsulated pancreatic β-cells to treat type 1 diabetes. When MIN6 cells, a pancreatic β-cell line, were encapsulated in poly(ethylene glycol)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin CC,Anseth KS

更新日期：2011-04-19 00:00:00