Abstract:
:The transition from fully developed CD4+CD8- single-positive (SP) thymocytes into fully mature recirculating peripheral T cells is both poorly understood with regard to the expression of restricted isoforms (CD45R) of the leukocyte common antigen and in terms of T cell function. The present investigation monitored the extrathymic development of CD4+CD8- SP thymocytes in euthymic recipients using allotype-marked donor cells and monoclonal antibody OX22 which recognizes an epitope on the C exon of rat CD45R. We established that donor-derived cells in the blood 1 day later bore the phenotype of the injected SP thymocytes (CD4+ Thy-1+ CD45RC-). T cells with the identical phenotype were also present in the thoracic duct lymph of uninjected rats, suggesting that the Thy-1+ CD45RC- T cells represent recent thymic emigrants (RTE) which have migrated to the periphery of their own accord. During extrathymic maturation donor-derived peripheral RTE lost Thy-1 within 3 days and expressed the CD45RC+ high molecular weight isoform by day 7; between days 8 and 14 a proportion (25%-30%) of the donor cells once again lost the high molecular weight isoform (CD45RC-). The transition of SP (CD45RC-) thymocytes to fully mature CD45RC+ CD4 T cells via intermediate peripheral RTE was accompanied at each stage by an increased ability of the maturing T cells to induce skin allograft rejection. Unexpectedly, the subsequent loss of the high molecular weight isoform, following presumed antigen encounter, was associated with a significant reduction in the ability of this Thy-1-CD45RC- subpopulation to effect graft rejection. The cyclic expression of CD45RC isoforms on both immature and mature CD4 T cells and the fact that the low molecular weight isoform was found in the periphery on both RTE (unquestionably naive) and antigen-experienced CD4 T cells, makes it unlikely that this isoform uniquely identifies memory T cells, at least in the rat.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Yang CP,Bell EBdoi
10.1002/eji.1830220913keywords:
subject
Has Abstractpub_date
1992-09-01 00:00:00pages
2261-9issue
9eissn
0014-2980issn
1521-4141journal_volume
22pub_type
杂志文章abstract::HLA-A2.1-binding peptides (n = 38) were screened for immunogenicity with human peripheral blood mononuclear cells in cytotoxic T lymphocyte (CTL) induction experiments in vitro and with splenocytes from HLA-A2.1/Kb transgenic mice following immunization in vivo. These data were compiled and analyzed to determine the l...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830260115
更新日期:1996-01-01 00:00:00
abstract::Membrane-bound immunoglobulins of the IgM and IgD class are expressed on the B cell surface in association with a disulfide-linked heterodimer consisting of alpha and beta subunits. While the alpha component of the IgM antigen receptor (IgM-alpha, 34 kDa) is encoded by the B cell-specific gene mb-1, the gene coding fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830211012
更新日期:1991-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830111109
更新日期:1981-11-01 00:00:00
abstract::The simultaneous detection of Ig and theta on the surface of mouse lymphocytes permits the detection of four cell types (Ig-theta-, Ig-theta+, Ig+theta-, and Ig+theta+) which also have distinct electrophoretic profiles. All types are present in variable proportions in all lymphoid organs studied. Results obtained in c...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830050211
更新日期:1975-02-01 00:00:00
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journal_title:European journal of immunology
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更新日期:1995-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160314
更新日期:1986-03-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis, an experimental murine model for multiple sclerosis, is induced by stimulation of myelin-specific T lymphocytes. Myelin oligodendrocyte glycoprotein (MOG), a minor component of myelin proteins, is a potent autoantigen which contributes extensively to the anti-myelin response....
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:European journal of immunology
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doi:10.1002/eji.201142242
更新日期:2012-05-01 00:00:00
abstract::The nonobese diabetic (NOD) mouse is a relevant model for studying human insulin-dependent diabetes mellitus (IDDM). The selective destruction of insulin-secreting cells in this model is subsequent to an autoimmune reaction directed towards the beta cells inside the islets of Langerhans of the pancreas. Given the key ...
journal_title:European journal of immunology
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doi:10.1002/eji.1830210511
更新日期:1991-05-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830171021
更新日期:1987-10-01 00:00:00
abstract::Rat CD4+ T cells are divided phenotypically by the anti-CD45RC monoclonal antibody OX22 into subsets with contrasting functions. Stimulation of T cells in vitro is known to induce a change in isoform from CD45RC+ to CD45RC-. We have investigated the in vitro conditions which promote a switch in isoform in the opposite...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230117
更新日期:1993-01-01 00:00:00
abstract::The initiation of CD8(+) T cell (CTL) immune responses can occur via cross-priming. Recent data suggested a relationship between cross-presentation and immunodominance of epitope-specific T cells. To test this association, we evaluated the efficacy of cross-presentation for several virus epitopes in vitro and examined...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939973
更新日期:2010-08-01 00:00:00
abstract::Naive and memory CD4 T cells differ in cell surface phenotype, function, activation requirements, and modes of regulation. To investigate the molecular bases for the dichotomies between naive and memory CD4 T cells and to understand how the T cell receptor (TCR) directs diverse functional outcomes, we investigated pro...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270838
更新日期:1997-08-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830130611
更新日期:1983-06-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270219
更新日期:1997-02-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324076
更新日期:2003-08-01 00:00:00
abstract::Multiple myeloma is a fatal B cell malignancy characterized by the accumulation of plasma cells within the bone marrow. IL-6 is a major survival factor for myeloma cells. Bcl-2 protein family regulates pathways to apoptosis that are activated upon growth factor deprivation. Pro-apoptotic proteins that have only a sing...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200424981
更新日期:2004-11-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
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abstract::CBA/J mice immunized with thyroglobulin (Tg) develop an experimental autoimmune thyroiditis (EAT) with lymphocytic infiltration of the thyroid glands, autoantibodies to Tg and occurrence of EAT-specific T cells. When these mice were treated for 4 weeks after immunization with 1 mg/week of a monoclonal antibody (mAb) t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230143
更新日期:1993-01-01 00:00:00
abstract::Stimulation of T cells via the T cell receptor (TCR) leads to an increase intracellular in free Ca2+ levels ([Ca2+]i) and the activation of the MAP kinase signaling pathway. This study analyzes for the first time Ca2+ fluxes in naive cytotoxic T cells stimulated with full agonists, partial agonists, or antagonists. Fo...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101205
更新日期:1980-12-01 00:00:00
abstract::Pre-B cells which show a delayed development of responsiveness to the B cell mitogen lipopolysaccharide (LPS) are found in murine embryonic blood between day 10 and 14 of gestation. The highest frequency of pre-B cells in embryonic blood is found at day 12 of gestation. All pre-B cells of embryonic blood, isolated at ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830101007
更新日期:1980-10-01 00:00:00
abstract::Antigen-free (AGF) and germ-free (GF) mice, although essentially free of serum IgG, maintain normal levels of circulating IgM. Using a quantitative immunoblot assay, we have now analyzed the repertoire of serum IgM from AGF, GF, and specific pathogen-free (SPF) BALB/c mice, on large panels of natural antigens from hom...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830270635
更新日期:1997-06-01 00:00:00
abstract::The present study tests whether the specific inhibition of helper T (Th) cell (and T hybridomas) by suppressor T (Ts) cells is a phenotypic trait of Th cells correlating with their acquired specificity for antigen/major histocompatibility complex or a genotypic trait not related to selection of the T cell repertoire f...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830180415
更新日期:1988-04-01 00:00:00
abstract::The crucial role of interleukin (IL)-4 in the induction of murine IgG1 and IgE responses, which are coupled through the process of sequential isotype switching, has been well documented. Whereas IL-4 is obligatory for the induction of IgE responses, it enhances IgG1 responses. In this study, using neutralizing antibod...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240624
更新日期:1994-06-01 00:00:00
abstract::The 20S proteasome is a protease complex of functional importance for antigen processing. Two of the 14 proteasome subunits, delta and MB1, can be replaced by the major histocompatibility complex (MHC)-encoded and interferon-gamma (IFN-gamma)-inducible subunits LMP2 and LMP7, respectively. LMP2 and LMP7 alter the clea...
journal_title:European journal of immunology
pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201142270
更新日期:2012-10-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141690
更新日期:2012-04-01 00:00:00
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journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830170305
更新日期:1987-03-01 00:00:00