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Protein kinase A-dependent coupling of mouse prostacyclin receptors to Gi is cell-type dependent.

Abstract：

:The ability of the prostacyclin (IP) receptor agonist cicaprost to activate Gs-, Gq/11- and Gi-mediated cell signalling pathways has been examined in Chinese hamster ovary (CHO) cells and human embryonic kidney 293 (HEK 293) cells expressing the cloned human (hIP) or mouse (mIP) prostacyclin receptor, and compared with data from NG108-15 and SK-N-SH cells that endogenously express rat/mouse and human IP receptors, respectively. Cicaprost stimulated [3H]cyclic AMP production with EC50 values of 1.5-22 nM, and stimulated [3H]inositol phosphate production (EC50 values 49-457 nM) in all but the SK-N-SH cells. Cicaprost failed to inhibit forskolin-stimulated [3H]cyclic AMP production in any of these cell lines. Therefore, although both human and mouse IP receptors couple to Gs and Gq/11-mediated signalling pathways in a cell type-dependent manner, we could find no evidence for IP receptor coupling to Gi.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Chow KB,Jones RL,Wise H

doi

10.1016/s0014-2999(03)02006-5

keywords:

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

7-13

issue

1

eissn

0014-2999

issn

1879-0712

pii

S0014299903020065

journal_volume

474

pub_type

杂志文章

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