Abstract：
BACKGROUND AND AIMS:Human VAT-1 (hVAT-1) is a homologue of the synaptic vesicle membrane protein of Torpedo californica. Its coding gene is located near the BRCA1 locus and thus hVAT-1 may be linked to an inherited predisposition to breast and ovary cancer. However, the hVAT-1 protein expression pattern in normal epithelial tissues such as skin, mammary gland and ovary, as well as in tumours of the mammary gland and ovary, has not been studied. METHODS:To address this issue, an immunohistological analysis of biopsies of normal epidermis and lesional epidermis of bullous pemphigoid and pemphigus vulgaris patients was undertaken. RESULTS:hVAT-1-expression was observed in basal keratinocytes of lesional epidermis of bullous pemphigoid patients but not in normal epidermis or in lesional epidermis of pemphigus vulgaris patients. Moreover, hVAT-1 expression in HaCaT cells was found to be calcium-dependent. Normal and malignant mammary and ovary epithelium were found to be hVAT-1-negative. CONCLUSIONS:Our results indicate that hVAT-1 exerts a specific function in keratinocyte physiology, in particular in calcium-regulated processes, with no evident deregulation in malignancies of the breast and ovary.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Koch J,Foekens J,Timmermans M,Fink W,Wirzbach A,Kramer MD,Schaefer BMdoi
10.1007/s00403-003-0421-8keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
203-10issue
5eissn
0340-3696issn
1432-069Xjournal_volume
295pub_type
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journal_title：Archives of dermatological research
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journal_title：Archives of dermatological research
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journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
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journal_title：Archives of dermatological research
pub_type： 杂志文章
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pub_type： 杂志文章
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