Abstract:
:Transcriptional activator proteins in bacteria often operate by interaction with the C-terminal domain of the alpha-subunit of RNA polymerase (RNAP). Here we report the discovery of an "anti-alpha" factor Spx in Bacillus subtilis that blocks transcriptional activation by binding to the alpha-C-terminal domain, thereby interfering with the capacity of RNAP to respond to certain activator proteins. Spx disrupts complex formation between the activator proteins ResD and ComA and promoter-bound RNAP, and it does so by direct interaction with the alpha-subunit. ResD- and ComA-stimulated transcription requires the proteolytic elimination of Spx by the ATP-dependent protease ClpXP. Spx represents a class of transcriptional regulators that inhibit activator-stimulated transcription by interaction with alpha.
journal_name
Proc Natl Acad Sci U S Aauthors
Nakano S,Nakano MM,Zhang Y,Leelakriangsak M,Zuber Pdoi
10.1073/pnas.0637648100keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
4233-8issue
7eissn
0027-8424issn
1091-6490pii
0637648100journal_volume
100pub_type
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