Abstract:
:Living cells regulate the activity of their ion channels through a process known as gating. To open the pore, protein conformational changes must occur within a channel's membrane-spanning ion pathway. KcsA and MthK, closed and opened K(+) channels, respectively, reveal how such gating transitions occur. Pore-lining 'inner' helices contain a 'gating hinge' that bends by approximately 30 degrees. In a straight conformation four inner helices form a bundle, closing the pore near its intracellular surface. In a bent configuration the inner helices splay open creating a wide (12 A) entryway. Amino-acid sequence conservation suggests a common structural basis for gating in a wide range of K(+) channels, both ligand- and voltage-gated. The open conformation favours high conduction by compressing the membrane field to the selectivity filter, and also permits large organic cations and inactivation peptides to enter the pore from the intracellular solution.
journal_name
Naturejournal_title
Natureauthors
Jiang Y,Lee A,Chen J,Cadene M,Chait BT,MacKinnon Rdoi
10.1038/417523akeywords:
subject
Has Abstractpub_date
2002-05-30 00:00:00pages
523-6issue
6888eissn
0028-0836issn
1476-4687pii
417523ajournal_volume
417pub_type
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