Abstract:
:The availability of methods for the amplification of nucleic acid sequences allows the genetic analysis in vitro of the structural and functional properties of many nucleic acids. We have now developed an in vitro selection and amplification system, and used it to analyse the self-splicing Tetrahymena ribozyme. A much wider range of selective conditions can be used in vitro than is possible with standard in vivo methods, and many more variants can be handled in vitro than in vivo. This method can be used to isolate the wild-type ribozyme, and structural variants that are as active as the wild type, from a pool of over 250,000 variants in only three cycles of selection and amplification.
journal_name
Naturejournal_title
Natureauthors
Green R,Ellington AD,Szostak JWdoi
10.1038/347406a0subject
Has Abstractpub_date
1990-09-27 00:00:00pages
406-8issue
6291eissn
0028-0836issn
1476-4687journal_volume
347pub_type
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