Abstract：
:Class-V myosin proceeds along actin filaments with large ( approximately 36 nm) steps. Myosin-V has two heads, each of which consists of a motor domain and a long (23 nm) neck domain. In accordance with the widely accepted lever-arm model, it was suggested that myosin-V steps to successive (36 nm) target zones along the actin helical repeat by tilting its long neck (lever-arm). To test this hypothesis, we measured the mechanical properties of single molecules of myosin-V truncation mutants with neck domains only one-sixth of the native length. Our results show that the processivity and step distance along actin are both similar to those of full-length myosin-V. Thus, the long neck domain is not essential for either the large steps or processivity of myosin-V. These results challenge the lever-arm model. We propose that the motor domain and/or the actomyosin interface enable myosin-V to produce large processive steps during translocation along actin.
journal_name
Naturejournal_title
Natureauthors
Tanaka H,Homma K,Iwane AH,Katayama E,Ikebe R,Saito J,Yanagida T,Ikebe Mdoi
10.1038/415192akeywords:
subject
Has Abstractpub_date
2002-01-10 00:00:00pages
192-5issue
6868eissn
0028-0836issn
1476-4687pii
415192ajournal_volume
415pub_type
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