Abstract:
:Dogs with immunoglobulin E (IgE) allergy for ragweed that are sensitized by intrapulmonary exposure to ragweed can be used to study the pulmonary immune response that is important in allergic asthma. Using this model, we tested the hypothesis that T lymphocytes are activated locally in the airways shortly after allergen exposure of the lungs. The airways of six allergic dogs and three non-allergic dogs were exposed to ragweed by segmental allergen challenge (SAC). T-cell subsets and T-cell activation in blood and bronchoalveolar lavage (BAL) fluid were measured by flow cytometry before SAC and at 4, 24 and 72 hr thereafter. SAC caused a statistically significant increase in the percentage of major histocompatibility complex (MHC) class II-positive CD4 and CD8 T cells in BAL fluid and a significant increase in the mean fluorescent activity of MHC class II from 4 hr after SAC onward. This activation was significantly different from that found in cells from lung lobes challenged with saline, or from lung lobes in non-allergic dogs challenged with ragweed. The percentage of CD45RA(bright) CD8 cells increased significantly in allergic dogs after both ragweed and saline challenges. This was significantly higher than in non-allergic dogs. We conclude that T-cell activation in the airways of dogs can be measured after in vivo activation of the cells by measuring MHC class II and CD45RA expression in BAL fluid T cells. Furthermore, in allergic dogs, T cells are activated locally in the lungs within 4 hr after exposure to ragweed allergen. These results suggest a role for T lymphocytes in the development of late-phase allergic reactions in the airways.
journal_name
Immunologyjournal_title
Immunologyauthors
Out TA,Wang SZ,Rudolph K,Bice DEdoi
10.1046/j.1365-2567.2002.01383.xkeywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
499-508issue
4eissn
0019-2805issn
1365-2567pii
1383journal_volume
105pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Rabbit IgG and its Fab, Fc and pFc' fragments, prepared by papain or peptic digestion, were assayed for binding to homologous peritoneal macrophages. The binding affinity of IgG for the peritoneal macrophages (Ka = 5.9 +/- 1.6 x 10(5) L/M) was comparable to that recorded with alveolar macrophages (7.6 +/- 1.8 x 10(5) ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::Factors that influence the generation and maintenance of memory CD8+ T cells are not fully understood. The homeostasis of memory T cells is highly dynamic and tightly regulated by various stimuli, including cytokines and antigen-major histocompatibility complex ligands. We characterized the hepatitis C virus (HCV)-spe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2003.01769.x
更新日期:2003-12-01 00:00:00
abstract::Cytotoxic T-lymphocyte antigen-4 (CTLA-4) is an important molecule in the down-regulation of T-cell activation. A study was undertaken to evaluate the association of the CTLA-4 gene polymorphisms -319C/T, +49A/G, (AT)(n), CT60A/G and Jo31G/T with the levels of membrane CTLA-4 (mCTLA-4) and cytoplasmic CTLA-4 (cCTLA-4)...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2009.03083.x
更新日期:2009-09-01 00:00:00
abstract::Human haematopoietic progenitor/stem cells (HPCs) differentiate into functional T cells in the thymus through a series of checkpoints. A convenient in vitro system will greatly facilitate the understanding of T-cell development and future engineering of therapeutic T cells. In this report, we established a lentiviral ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.03013.x
更新日期:2009-09-01 00:00:00
abstract::Donor T cells are crucial for target organ injury in graft-versus-host disease (GVHD). We examined the effects of donor T cells on the target organs using a parent-into-F1 model of acute and chronic GVHD. Donor T cells showed engraftment in the spleen, small intestine and liver of mice with acute GVHD, causing typical...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01240.x
更新日期:2001-07-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II molecules present antigenic peptides derived from engulfed exogenous proteins to CD4(+) T cells. Exogenous antigens are processed in mature endosomes and lysosomes where acidic proteases reside and peptide-binding to class II alleles is favoured. Hence, maintenance of th...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03309.x
更新日期:2010-11-01 00:00:00
abstract::Interleukin-19 (IL-19) plays an important role in asthma by stimulating T helper type 2 (Th2) cytokine production. Interestingly, IL-4, a key Th2 cytokine, in turn up-regulates IL-19 expression in bronchial epithelial cells, so forming a positive feedback loop. In atopic dermatitis (AD), another Th2 disease closely re...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12339
更新日期:2014-12-01 00:00:00
abstract::To analyse the immunological mechanism of hepatocellular injury in hepatitis B virus (HBV) infection, the immunoreactivity of HBV-encoded antigens as a target for cytotoxic T lymphocyte (CTL) response was examined using recombinant vaccinia virus (RVV) expressing surface protein (S), precore/core protein (PC), and cor...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::Immunopotentiation has been demonstrated when Klebsiella O3 lipopolysaccharide (KO3 LPS), which possesses a linear mannan as the O-specific side chain, was injected subcutaneously into endotoxin resistant C3H/HeJ mice together with soluble protein antigens. The LPS exhibited significantly positive adjuvant effects on ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::Virus plaque assay (VPA) was utilized for the quantitative evaluation of activated lymphocytes. We examined what types of cells, especially which of activated T and non-T lymphocytes, were detected as infective centres after infection with vesicular stomatitis virus. Marked increases in DNA synthesis and in virus-plaq...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::The interleukin-2 receptor alpha chain (IL-2R alpha) is a T-cell growth factor receptor and is known to be induced in helper T cells by infection with human T-cell leukaemia virus type-1 (HTLV-1). The Rex protein of HTLV-1 has been shown to stabilize IL-2R alpha mRNA. Although the 3' untranslated region of many RNA ha...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::Antibody plaque-forming cells (FC) to the somatic antigens of Vibrio cholerae were enumerated in the spleen of mice after primary and secondary immunization with a heat-killed vaccine prepared from the vibrios. Immunocytes releasing both high efficiency IgM and low efficiency IgG antibody were readily detected using a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::Sialoadhesin (Sn, also known as Siglec-1 and CD169) is a macrophage-restricted cell surface receptor that is conserved across mammals. Sn is a member of the sialic acid-binding IgG-like lectin (Siglec) family of proteins characterized by affinity to specifically sialylated ligands, and under normal conditions is expre...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12042
更新日期:2013-03-01 00:00:00
abstract::The effects of cyclophosphamide (CY) on antigenic competition in IgE antibody production were studied in mice treated with the drug on different days and immunized with a mixture of two non-related antigens. Injection of 100 mg of CY/kg of body weight 3 days before or 6 days after immunization resulted in a partial or...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-09-01 00:00:00
abstract::The non-specific normal immunosuppressive protein (Nip) has been described in our laboratory and its biological activity was extensively studied. In the present study, further purification analyses of Nip were conducted. Fractionation of Nip by Ultrogel AcA 34 column resulted in peak (I) that displayed Nip activity in...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::To define the role of memory T cells in a non-persistent viral infection, we have delineated the phenotype of memory CD8+ T cells specific for influenza A virus (FluA; matrix protein M158-66) based on the expression of several memory/effector lineage markers and relevant chemokine receptors. We found a majority of Flu...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02135.x
更新日期:2005-06-01 00:00:00
abstract::Post-translational modifications of histone proteins are major mechanisms that modify chromatin structure and regulate gene expression in eukaryotes. Activation of histone acetyltransferases or inhibition of histone deacetylases (HDACs) is generally believed to allow chromatin to assume a more open state, permitting t...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02678.x
更新日期:2007-12-01 00:00:00
abstract::A monolayer depletion/adoptive immunization protocol that biased the immune response towards recognition of placental macrophage (pMO) antigens was established. BALB/c spleen cells immune to human pMO were adsorbed onto monolayers of the B-cell line QIMR-WIL. Monolayer-depleted or unfractionated cells were transferred...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Loss of ζ-associated protein 70 (Zap70) results in severe immunodeficiency in humans and mice because of the critical role of Zap70 in T-cell receptor (TCR) signalling. Here we describe a novel mouse strain generated by N-ethyl-N-nitrosourea mutagenesis, with the reduced protein stability (rps) mutation in Zap70. The ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12199
更新日期:2014-03-01 00:00:00
abstract::CD44, a cell adhesion molecule, exists in multiple isoforms that are generated by RNA alternative splicing. CD44 isoforms containing exon V6 (CD44 V6) have been associated with tumorigenesis and metastasis. We investigated the association between human B-cell activation and CD44 V6 isoform expression by analysing its ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
abstract::Human β-defensin 3 (hBD-3) activates antigen-presenting cells through Toll-like receptors (TLRs) 1/2. Several TLR1/2 agonists have been identified but little is known about how they might differentially affect cellular activation. We compared the effects of hBD-3 with those of another TLR1/2 agonist, Pam(3) CSK(4) , i...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03475.x
更新日期:2011-10-01 00:00:00
abstract::Iron-withholding by the chelating agent desferrioxamine abrogates the proliferative response of human peripheral blood mononuclear cells (PBMC) to phytohaemagglutinin (PHA). The present study investigated whether desferrioxamine operates late in the activation process or, as recently suggested, at an early stage, by i...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::The functional avidity of a cytotoxic T lymphocyte (CTL) is known to be a critical determinant of the efficacy with which it clears pathogens. High avidity cells, which are by definition highly sensitive to peptide antigen, are superior for elimination of viruses and tumours. Our studies have established the ability o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03440.x
更新日期:2011-07-01 00:00:00
abstract::We have previously demonstrated that immunoglobulin A (IgA)(-/-) knockout (KO) mice exhibit levels of susceptibility to influenza virus infection that are similar to those of their normal IgA(+/+) littermates. To understand the mechanism of this apparent mucosal immunity without IgA, immunoglobulin isotype and T helpe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.0019-2805.2001.01368.x
更新日期:2002-03-01 00:00:00
abstract::The current study sought to delineate the gene expression profile of the host response in the caecum and colon during acute infection with Clostridium difficile in a mouse model of infection, and to investigate the nature of the unfolded protein response in this process. The infected mice displayed a significant up-re...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12122
更新日期:2013-09-01 00:00:00
abstract::Down-regulated chemerin expression has been reported to correlate with poor prognosis of several types of cancer including melanoma. All-trans retinoic acid (atRA) is a potent inducer of chemerin, and we previously reported that atRA inhibited murine melanoma growth through enhancement of anti-tumor T-cell immunity. H...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13065
更新日期:2019-07-01 00:00:00
abstract::Interaction between CD27 and its ligand CD70 provides a second signal for T-cell proliferation and tumour necrosis factor-alpha (TNF-alpha) production. Whereas CD27 is broadly expressed during T-cell development, expression of CD70 in vivo is restricted. To determine when CD27 CD70 interactions can occur in immune rea...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00134.x
更新日期:1997-01-01 00:00:00
abstract::Various methods for measuring monocyte numbers and function in human blood are compared. The assays used were: phagocytosis of antibody-sensitized sheep red cells; staining for diffuse non-specific esterase activity; capacity to adhere to polystyrene and ability to lyse human A1 red cells sensitized with allogeneic an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-12-01 00:00:00
abstract::Innate immune responses vary in a circadian manner, and more recent investigations aim to understand the underlying molecular mechanisms. Cytokine production varies significantly over the course of a day depending on the time of stimulation by pathogens or Toll-like receptor ligands, and multiple signaling pathways li...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13053
更新日期:2019-06-01 00:00:00
abstract::Burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease with high incidence and mortality in South East Asia and northern Australia. To date there is no protective vaccine and antibiotic treatment is prolonged and not always effective. Most people living in endemic areas have been expose...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12709
更新日期:2017-05-01 00:00:00