Abstract：
:The binding of alpha-factor to its receptor (Ste2p) activates a G-protein-signaling pathway leading to conjugation of MATa cells of the budding yeast S. cerevisiae. We conducted a genetic screen to identify constitutively activating mutations in the N-terminal region of the alpha-factor receptor that includes transmembrane domains 1-5. This approach identified 12 unique constitutively activating mutations, the strongest of which affected polar residues at the cytoplasmic ends of transmembrane domains 2 and 3 (Asn84 and Gln149, respectively) that are conserved in the alpha-factor receptors of divergent yeast species. Targeted mutagenesis, in combination with molecular modeling studies, suggested that Gln149 is oriented toward the core of the transmembrane helix bundle where it may be involved in mediating an interaction with Asn84. These residues appear to play specific roles in maintaining the inactive conformation of the protein since a variety of mutations at either position cause constitutive receptor signaling. Interestingly, the activity of many mammalian G-protein-coupled receptors is also regulated by conserved polar residues (the E/DRY motif) at the cytoplasmic end of transmembrane domain 3. Altogether, the results of this study suggest a conserved role for the cytoplasmic end of transmembrane domain 3 in regulating the activity of divergent G-protein-coupled receptors.
journal_name
Geneticsjournal_title
Geneticsauthors
Parrish W,Eilers M,Ying W,Konopka JBkeywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
429-43issue
2eissn
0016-6731issn
1943-2631journal_volume
160pub_type
杂志文章相关文献
GENETICS文献大全abstract：:We used ends-in gene targeting to generate knockout mutations of the nucleosome assembly protein 1 (Nap1) gene in Drosophila melanogaster. Three independent targeted null-knockout mutations were produced. No wild-type NAP1 protein could be detected in protein extracts. Homozygous Nap1(KO) knockout flies were either em...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-02-01 00:00:00
abstract：:The N-terminal tail of Ndc80 is essential for kinetochore-microtubule binding in human cells but is not required for viability in yeast. We show that the yeast Ndc80 tail is required for timely mitotic progression and accurate chromosome segregation. The tail is essential when cells are limited for Dam1, demonstrating...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.143818
更新日期：2012-10-01 00:00:00
abstract：:Many insects feed on only one or a few types of host. These host specialists often evolve a preference for chemical cues emanating from their host and develop mechanisms for circumventing their host's defenses. Adaptations like these are central to evolutionary biology, yet our understanding of their genetics remains ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.154773
更新日期：2013-11-01 00:00:00
abstract：:The fixation of a beneficial allele in a population leaves a well-characterized signature in patterns of nucleotide variation at linked sites. This signature can be used to estimate the time since fixation from patterns of polymorphism in extant individuals. I introduce a method to assess the support in polymorphism d...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：:Positive selection on a new mutant allele can increase the frequencies of closely linked alleles (through hitchhiking), as well as create linkage disequilibrium between them. Because this disequilibrium is induced by the selected allele, one may be able to identify loci under selection by measuring the influence of a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:At least six nuclear loci are responsible for the genetic control of malate dehydrogenase (L-malate: NAD oxidoreductase; EC 1.1.1.37; MDH) in coleoptiles of maize. Three independently segregating loci (Mdh1, Mdh2, Mdh3) govern the production of MDH isozymes resistant to inactivation by ascorbic acid and found largely ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:Hybrid incompatibility can result from gene misregulation produced by divergence in trans-acting regulatory factors and their cis-regulatory targets. However, change in trans-acting factors may be constrained by pleiotropy, which would in turn limit the evolution of incompatibility. We employed a mechanistically expli...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.171397
更新日期：2014-12-01 00:00:00
abstract：:Of 313 motility-deficient mutants isolated from an LT2 his(amber) strain fixed in phase 1 by gene vh2(-), 25 regained motility when amber or ochre suppressors were introduced, in F' factors or by transduction. The fla mutants (23 amber, 1 ochre) fell in complementation groups A, B, C, F, K, a new group, M, and at leas...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-02-01 00:00:00
abstract：:We have determined the complete nucleotide sequence of the mitochondrial DNA (mtDNA) of the dogfish, Scyliorhinus canicula. The 16,697-bp-long mtDNA possesses a gene organization identical to that of the Osteichthyes, but different from that of the sea lamprey Petromyzon marinus. The main features of the mtDNA of oste...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:The Drosophila PS1 and PS2 integrins are required to maintain the connection between the dorsal and ventral wing epithelia. If alphaPS subunits are inappropriately expressed during early pupariation, the epithelia separate, causing a wing blister. Two lines of evidence indicate that this apparent loss-of-function phen...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:In many plant species, exposure to a prolonged period of cold during the winter promotes flowering in the spring, a process termed vernalization. In Arabidopsis thaliana, the vernalization requirement of winter-annual ecotypes is caused by the MADS-box gene FLOWERING LOCUS C (FLC), which is a repressor of flowering. D...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.069336
更新日期：2007-05-01 00:00:00
abstract：:The prion-like behavior of Sup35p, the eRF3 homolog in the yeast Saccharomyces cerevisiae, mediates the activity of the cytoplasmic nonsense suppressor known as [PSI(+)]. Sup35p is divided into three regions of distinct function. The N-terminal and middle (M) regions are required for the induction and propagation of [...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:We have amplified and sequenced the gene encoding Esterase-P (Est-P) in 10 strains of Drosophila melanogaster. Three premature termination codons occur in the coding region of the gene in two strains. This observation, together with other indirect evidence, leads us to propose that Est-P may be a pseudogene in D. mela...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:We have identified a new locus involved in gibberellin (GA) signal transduction by screening for suppressors of the Arabidopsis thaliana GA biosynthetic mutant gal-3. The locus is named RGA for repressor of gal-3. Based on the recessive phenotype of the digenic rga/gal-3 mutant, the wild-type gene product of RGA is pr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Transcription of the Saccharomyces MAL structural genes is induced 40-fold by maltose and requires the MAL-activator and maltose permease. To identify additional players involved in regulating MAL gene expression, we carried out a genetic selection for MAL constitutive mutants. Strain CMY4000 containing MAL1 and integ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.029611
更新日期：2004-10-01 00:00:00
abstract：:In Drosophila melanogaster, the endogenous retrovirus gypsy is repressed by the functional alleles (restrictive) of an as-yet-uncloned heterochromatic gene called flamenco. Using gypsy-lacZ transcriptional fusions, we show here that this repression takes place not only in the follicle cells of restrictive ovaries, as ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.3.1313
更新日期：2004-03-01 00:00:00
abstract：:Genetic and developmental analysis of an X-linked vital locus vnd was undertaken. Embryos hemizygous for the original allele vnd did not hatch and exhibited a disorganized ventral nervous system (VNS). The mutation maps in the region 1B6-7 to 1B9-10, a subregion of an area previously shown to be essential to normal ne...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:We have examined the stability of the tandemly repeated genes that encode the ribosomal RNA in Coprinus cinereus. These genes are contained within two linked HindIII fragments in a 3.0-Mb chromosome. We monitored the size of these fragments in both mitotic and meiotic segregants using the contour-clamped homogeneous e...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:The Sh2/A1 orthologous region of maize, rice, and sorghum contains five genes in the order Sh2, X1, X2, and two A1 homologs in tandem duplication. The Sh2 and A1 homologs are separated by approximately 20 kb in rice and sorghum and by approximately 140 kb in maize. We analyzed the fate of the Sh2/A1 region in large-ge...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Fluorescent in situ hybridization was used to examine the distribution of six abundant long terminal repeat (LTR) retroelements, Opie, Huck, Cinful-1, Prem-2/Ji, Grande, and Tekay/Prem-1 on maize pachytene chromosomes. Retroelement staining in euchromatin was remarkably uniform, even when we included the structurally ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：:Positive selection in genes and genomes can point to the evolutionary basis for differences among species and among races within a species. The detection of positive selection can also help identify functionally important protein regions and thus guide protein engineering. Many existing tests for positive selection ar...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.074732
更新日期：2007-08-01 00:00:00
abstract：:We have isolated 15 spontaneous mutants resistant to one or several antibiotics like chloramphenicol, erythromycin and spiramycin. We have shown by several criteria that all of them result from mutations localized in the mitochondrial DNA. The mutations have been mapped by allelism tests and by two- and three-factor c...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-12-01 00:00:00
abstract：:The meiotic properties of paracentric inversion heterozygotes have been well studied in insects and plants, but not in mammalian species. In essence, a single meiotic recombination event within the inverted region results in the formation of a dicentric chromatid, which usually breaks or is stretched between the two d...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:We study levels of X-linked vs. autosomal diversity using a model developed to analyze the hitchhiking effect. Repeated bouts of hitchhiking are thought to lower X-linked diversity for two reasons: first, because sojourn times of beneficial mutations are shorter on the X, and second, because adaptive substitutions may...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.030999
更新日期：2004-12-01 00:00:00
abstract：:The Triplo-lethal locus (Tpl) is unique in its dosage sensitivity; no other locus in Drosophila has been identified that is lethal when present in three doses. Tpl is also haplo-lethal, and its function is still a mystery. Previous workers have found it nearly impossible to mutationally inactive Tpl other than by comp...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:In contrast to our growing understanding of patterns of additive genetic variance in single- and multi-trait combinations, the relative contribution of nonadditive genetic variance, particularly dominance variance, to multivariate phenotypes is largely unknown. While mechanisms for the evolution of dominance genetic v...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.175489
更新日期：2015-05-01 00:00:00
abstract：:Statistical associations between phenotypic traits often result from shared developmental processes and include both covariation between the trait values and more complex associations between higher moments of the joint distribution of traits. In this article, an analytical technique for calculating the covariance bet...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.1.513
更新日期：2004-01-01 00:00:00
abstract：:The resistance of rice to its bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo) has both qualitative and quantitative components that were investigated using three near-isogenic line sets for four resistance (R) genes (Xa4, xa5, xa13, and Xa21) and 12 Xoo races. Our results indicate that these two resistan...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:Isogenic lines, in which chromosomes sampled from natural populations of C. melanogaster are substituted into a common genetic background, were used to detect and partially characterize autosomal factors that affect the activities of the two pentose phosphate pathway enzymes, glucose 6-phosphate dehydrogenase (G6PD) a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:Environmental toxicants are chemicals that negatively affect human health. Although there are numerous ways to limit exposure, the ubiquitous nature of certain environmental toxicants makes it impossible to avoid them entirely. Consequently, scientists are continuously working toward developing strategies for combatin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303577
更新日期：2020-12-01 00:00:00
