Abstract：
:The prion-like behavior of Sup35p, the eRF3 homolog in the yeast Saccharomyces cerevisiae, mediates the activity of the cytoplasmic nonsense suppressor known as [PSI(+)]. Sup35p is divided into three regions of distinct function. The N-terminal and middle (M) regions are required for the induction and propagation of [PSI(+)] but are not necessary for translation termination or cell viability. The C-terminal region encompasses the termination function. The existence of the N-terminal region in SUP35 homologs of other fungi has led some to suggest that this region has an adaptive function separate from translation termination. To examine this hypothesis, we sequenced portions of SUP35 in 21 strains of S. cerevisiae, including 13 clinical isolates. We analyzed nucleotide polymorphism within this species and compared it to sequence divergence from a sister species, S. paradoxus. The N domain of Sup35p is highly conserved in amino acid sequence and is highly biased in codon usage toward preferred codons. Amino acid changes are under weak purifying selection based on a quantitative analysis of polymorphism and divergence. We also conclude that the clinical strains of S. cerevisiae are not recently derived and that outcrossing between strains in S. cerevisiae may be relatively rare in nature.
journal_name
Geneticsjournal_title
Geneticsauthors
Jensen MA,True HL,Chernoff YO,Lindquist Skeywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
527-35issue
2eissn
0016-6731issn
1943-2631journal_volume
159pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Mapping of expression quantitative trait loci (eQTL) is a powerful means for elucidating the genetic architecture of gene regulation. Yet, eQTL mapping has not been applied toward investigating the regulation architecture of genes involved in the process of population divergence, ultimately leading to speciation event...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.086306
更新日期：2008-08-01 00:00:00
abstract：:We consider the effect on the distribution of pairwise differences between mitochondrial DNA sequences of the incorporation into the underlying population genetics model of two particular effects that seem realistic for human populations. The first is that the population size was roughly constant before growing to its...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since approximately 10% of the genes are expressed in the male gametophyte and approximately 9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduce...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.090852
更新日期：2009-04-01 00:00:00
abstract：:The time of the pre-meiotic S-period was determined by (32)P incorporation in synchronously germinating zygospores of Chlamydomonas reinhardi at six and one-half to seven hours after the beginning of germination. Phenethyl alcohol treatment caused death of zygospores at a period one hour before the S-period, and also ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-01-01 00:00:00
abstract：:Infantile neuronal ceroid lipofuscinosis (INCL) is a pediatric neurodegenerative disease caused by mutations in the human CLN1 gene. CLN1 encodes palmitoyl-protein thioesterase 1 (PPT1), suggesting an important role for the regulation of palmitoylation in normal neuronal function. To further elucidate Ppt1 function, w...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.067983
更新日期：2007-05-01 00:00:00
abstract：:Cornell Control White Leghorn chicks were grown in a common environment to five weeks of age and selected for fast and slow gain in body weight from five to nine weeks of age at two temperatures, 21.1 degrees (cold) and 32.2 degrees (hot), during which time a constant 50% relative humidity was maintained. All lines we...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:In Arabidopsis thaliana, significant efforts to determine the extent of genomic variation between phenotypically divergent accessions are under way, but virtually nothing is known about variation at the transcription level. We used microarrays to examine variation in transcript abundance among three inbred lines and t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.041509
更新日期：2005-11-01 00:00:00
abstract：:Deficiencies encompassing part or all of the X heterochromatin of Drosophila melanogaster have been linked to three abnormalities in male meiosis and spermatogenesis: X-Y nondisjunction, skewed sperm recovery ratios favoring sperm with reduced chromatin content, and sterility in males carrying either a Y-autosome tran...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Variations in K(+) channel composition allow for differences in cell excitability and, at an organismal level, provide flexibility to behavioral regulation. When the function of a K(+) channel is disrupted, the remaining K(+) channels might incompletely compensate, manifesting as abnormal organismal behavior. In this ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.137455
更新日期：2012-03-01 00:00:00
abstract：:From the analysis of restriction maps of the Amy region in eight sibling species belonging to the Drosophila melanogaster species subgroup, we herein show that the patterns of duplication of the Amy gene are almost the same in all species. This indicates that duplication occurred before speciation within this species ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:There is an increasing awareness that selection affecting linked neutral sites strongly influences on how diversity is distributed across the genome. In particular, linked selection is likely involved in the formation of heterogenous landscapes of genetic diversity, including genomic regions with locally reduced effec...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.301991
更新日期：2019-05-01 00:00:00
abstract：:The aim of many genetic studies is to locate the genomic regions (called quantitative trait loci, QTL) that contribute to variation in a quantitative trait (such as body weight). Confidence intervals for the locations of QTL are particularly important for the design of further experiments to identify the gene or genes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.061549
更新日期：2006-09-01 00:00:00
abstract：:The neurogenic loci comprise a small group of genes which are required for proper division between the neural and epidermal pathways of differentiation within the neuroectoderm. Loss of neurogenic gene function results in the misrouting of prospective epidermal cells into neuroblasts. A molecular analysis of the neuro...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:The distribution of fitness effects (DFE) of new mutations is of fundamental importance in evolutionary genetics. Recently, methods have been developed for inferring the DFE that use information from the allele frequency distributions of putatively neutral and selected nucleotide polymorphic variants in a population s...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.131730
更新日期：2011-12-01 00:00:00
abstract：:The Drosophila piwi gene is the founding member of the only known family of genes whose function in stem cell maintenance is highly conserved in both animal and plant kingdoms. piwi mutants fail to maintain germline stem cells in both male and female gonads. The identification of piwi-interacting genes is essential fo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:Nonallelic gene conversion has been proposed as a major force in homogenizing the sequences of paralogous genes. In this work, we investigate the extent and characteristics of gene conversion among gene families in nine species of the genus Drosophila. We carried out a genome-wide study of 2855 gene families (includin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.115444
更新日期：2010-05-01 00:00:00
abstract：:Analysis of raw pooled data from distinct studies of a single question generates a single statistical conclusion with greater power and precision than conventional metaanalysis based on within-study estimates. However, conducting analyses with pooled genetic data, in particular, is a daunting task that raises importan...
journal_title：Genetics
pub_type： 杂志文章,meta分析
doi：
更新日期：2001-11-01 00:00:00
abstract：:The Saccharomyces cerevisiae GAL/MEL regulon genes are normally induced within minutes of galactose addition, but gal3 mutants exhibit a 3-5-day induction lag. We have discovered that this long-term adaptation (LTA) phenotype conferred by gal3 is complemented by multiple copies of the GAL1 gene. Based on this result a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:Type II topoisomerases are essential ATP-dependent homodimeric enzymes required for transcription, replication, and chromosome segregation. These proteins alter DNA topology by generating transient enzyme-linked double-strand breaks for passage of one DNA strand through another. The central role of type II topoisomera...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.144006
更新日期：2012-11-01 00:00:00
abstract：:The Genetics Society of America's (GSA) Edward Novitski Prize is awarded to researchers who have solved challenging problems in genetics through experiments that demonstrate exceptional creativity and ingenuity. Job Dekker of the University of Massachusetts Medical School has been selected for the 2018 award in recogn...
journal_title：Genetics
pub_type： 传,历史文章,杂志文章
doi：10.1534/genetics.118.301641
更新日期：2018-11-01 00:00:00
abstract：:Dopamine acts through two classes of G protein-coupled receptor (D1-like and D2-like) to modulate neuron activity in the brain. While subtypes of D1- and D2-like receptors are coexpressed in many neurons of the mammalian brain, it is unclear how signaling by these coexpressed receptors interacts to modulate the activi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.128512
更新日期：2011-07-01 00:00:00
abstract：:Zinc plays many critical roles in biological systems: zinc bound to proteins has structural and catalytic functions, and zinc is proposed to act as a signaling molecule. Because zinc deficiency and excess result in toxicity, animals have evolved sophisticated mechanisms for zinc metabolism and homeostasis. However, th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.084384
更新日期：2008-06-01 00:00:00
abstract：:Selective sweeps are typically associated with a local reduction of genetic diversity around the adaptive site. However, selective sweeps can also quickly carry neutral mutations to observable population frequencies if they arise early in a sweep and hitchhike with the adaptive allele. We show that the interplay betwe...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.138461
更新日期：2012-06-01 00:00:00
abstract：:A study of spontaneous mutation in Arabidopsis thaliana was initiated from a single inbred Columbia founder; 120 lines were established and advanced 17 generations by single-seed descent. Here, we report an assay of reproductive traits in a random set of 40 lines from generations 8 and 17, grown together at the same t...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:A specific host-pathogen interaction exists between Caenorhabditis elegans and the gram-positive bacterium Microbacterium nematophilum. This bacterium is able to colonize the rectum of susceptible worms and induces a defensive tail-swelling response in the host. Previous mutant screens have identified multiple loci th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.060087
更新日期：2007-02-01 00:00:00
abstract：:The mitochondrial genome of Neurospora is usually found in a single covalently closed circular 62-kbp DNA molecule. We report here that the mitochondrial genome of a phenotypic revertant of a stopper mutant (stp-ruv) is contained primarily in two separate, nonoverlapping, autonomously replicating circular chromosomes....
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:An elevated mutation rate can provide cells with a source of mutations to adapt to changing environments. We identified a negative epistatic interaction involving naturally occurring variants in the MLH1 and PMS1 mismatch repair (MMR) genes of Saccharomyces cerevisiae We hypothesized that this MMR incompatibility, cre...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.199513
更新日期：2017-04-01 00:00:00
abstract：:The parasitoid wasp Nasonia vitripennis is an emerging model organism for developmental and behavioral genetics. It reproduces by haplodiploidy; males typically develop parthenogenetically from haploid eggs and females from fertilized diploid eggs. A polyploid mutant strain is available in which females are triploid a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.044743
更新日期：2006-02-01 00:00:00
abstract：:Antisuppressor mutations in the eEF1A gene of Podospora anserina were previously shown to impair ascospore formation, to drastically increase life span, and to permit the development of the Crippled Growth degenerative process. Here, we show that eEF1A controls ascospore formation through accuracy level maintenance. E...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-08-01 00:00:00
abstract：:We consider some practical statistical issues in QTL analysis where several crosses originate in multiple inbred parents. Our results show that ignoring background polygenic variation in different crosses may lead to biased interval mapping estimates of QTL effects or loss of efficiency. Threshold and power approximat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
