Abstract：
:The gastrulation defective (gd) locus encodes a novel serine protease that is involved in specifying the dorsal-ventral axis during embryonic development. Mutant alleles of gd have been classified into three complementation groups, two of which exhibit strong interallelic (intragenic) complementation. To understand the molecular basis of this interallelic complementation, we examined the complementation behavior of additional mutant alleles and sequenced alleles in all complementation groups. The data suggest that there are two discrete functional domains of Gd. A two-domain model of Gd suggesting that it is structurally similar to mammalian complement factors C2 and B has been previously proposed. To test this model we performed SP6 RNA microinjection to assay for activities associated with various domains of Gd. The microinjection data are consistent with the complement factor C2/B-like model. Site-directed mutagenesis suggests that Gd functions as a serine protease. An allele-specific interaction between an autoactivating form of Snake (Snk) and a gd allele altered in the protease domain suggests that Gd directly activates Snk in a protease activation cascade. We propose a model in which Gd is expressed during late oogenesis and bound within the perivitelline space but only becomes catalytically active during embryogenesis.
journal_name
Geneticsjournal_title
Geneticsauthors
Ponomareff G,Giordano H,DeLotto Y,DeLotto Rkeywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
635-45issue
2eissn
0016-6731issn
1943-2631journal_volume
159pub_type
杂志文章相关文献
GENETICS文献大全abstract：:In Drosophila melanogaster, the female sexual development of the soma and the germline requires the activity of the gene Sxl. The somatic cells need the function of the gene fl(2)d to follow the female developmental pathway, due to its involvement in the female-specific splicing of Sxl RNA. Here we report the analysis...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The Drosophila mei-S332 gene acts to maintain sister-chromatid cohesion before anaphase II of meiosis in both males and females. By isolating and analyzing seven new alleles and a deficiency uncovering the mei-S332 gene we have demonstrated that the onset of the requirement for mei-S332 is not until late anaphase I. A...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:A novel fine structure mapping method for quantitative traits is presented. It is based on Bayesian modeling and inference, treating the number of quantitative trait loci (QTLs) as an unobserved random variable and using ideas similar to composite interval mapping to account for the effects of QTLs in other chromosome...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Stem cells have a fascinating biology and offer great prospects for therapeutic applications, stimulating intense research on what controls their properties and behavior. Although there have been significant advances in our understanding of how local microenvironments, or niches, control the maintenance and activity o...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.108.098244
更新日期：2008-12-01 00:00:00
abstract：:In Saccharomyces cerevisiae, silencers flanking the HML and HMR loci initiate the establishment of transcriptional silencing. We demonstrate that the activity of a silencer pertaining to its potency and directionality is dependent on its genomic position. The context of the HML-E silencer is more permissive to silence...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.055525
更新日期：2006-09-01 00:00:00
abstract：:Although the role of U1 small nuclear RNAs (snRNAs) in 5' splice site recognition is well established, suppressor U1 snRNAs active in intact multicellular animals have been lacking. Here we describe suppression of a 5' splice site mutation in the Drosophila melanogaster white gene (wDR18) by compensatory changes in U1...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Spt3 of Saccharomyces cerevisiae is required for the normal transcription of many genes in vivo. Past studies have shown that Spt3 is required for both mating and sporulation, two events that initiate when cells are at G(1)/START. We now show that Spt3 is needed for two other events that begin at G(1)/START, diploid f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:We consider inference for demographic models and parameters based upon postprocessing the output of an MCMC method that generates samples of genealogical trees (from the posterior distribution for a specific prior distribution of the genealogy). This approach has the advantage of taking account of the uncertainty in t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.071910
更新日期：2007-09-01 00:00:00
abstract：:Theoretically, one of the most general benefits of sex is given by its function in facilitating selection against deleterious mutations. This advantage of sex may be deterministic if deleterious mutations affect the fitness of an individual in a synergistic way, i.e., if mutations increase each others' negative fitnes...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:Trichome development in Arabidopsis thaliana is a well-characterized model for the study of plant cell differentiation. Two genes that play an essential role in the initiation of trichome development are GL1 and TTG. Mutations in either gene prevent the initiation of most trichomes. The GL1 gene encodes a myb-related ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:Significant amounts of apoptosis take place during Drosophila development. The proapoptotic genes reaper (rpr), grim, and head involution defective (hid) are required for virtually all embryonic apoptosis. The proteins encoded by these genes share a short region of homology at their amino termini. The Drosophila IAP h...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:All living organisms require nutrient minerals for growth and have developed mechanisms to acquire, utilize, and store nutrient minerals effectively. In the aqueous cellular environment, these elements exist as charged ions that, together with protons and hydroxide ions, facilitate biochemical reactions and establish ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.112.147207
更新日期：2013-03-01 00:00:00
abstract：:We show by molecular analysis of behavioral and physiological mutants that the Drosophila Dmca1A calcium-channel alpha1 subunit is encoded by the cacophony (cac) gene and that nightblind-A and lethal(1)L13 mutations are allelic to cac with respect to an expanded array of behavioral and physiological phenotypes associa...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:We have analyzed pregastrulation cleavage patterns in Caenorhabditis elegans embryos homozygous for various chromosomal deficiencies. By two different estimates these deficiencies represent between 37 and 49% of the genome, including the entire X chromosome and substantial portions of each of the five autosomes. Among...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:We analyze nucleotide polymorphism data for a large number of loci in areas of normal to high recombination in Drosophila melanogaster and D. simulans (24 and 16 loci, respectively). We find a genome-wide, systematic departure from the neutral expectation for a panmictic population at equilibrium in natural population...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:In response to an external source of adenine, yeast cells repress the expression of purine biosynthesis pathway genes. To identify necessary components of this signalling mechanism, we have isolated mutants that are constitutively active for expression. These mutants were named bra (for bypass of repression by adenine...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:A plausible explanation for many MHC-linked diseases is lacking. Sequencing of the MHC class I region (coding units or full contigs) in several human and nonhuman primate haplotypes allowed an analysis of single nucleotide variations (SNV) across this entire segment. This diversity was not evenly distributed. It was r...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.057034
更新日期：2006-07-01 00:00:00
abstract：:A multi-locus model for complete positive assortative mating is discussed. For a two-locus model, if the gene frequencies for the two loci are different, as they are likely to be, it is shown that in equilibrium the population is not composed of only two homozygous types, as is usually thought. The limiting distributi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-12-01 00:00:00
abstract：:Differential gene expression across cell types underlies development and cell physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates to the difficulty to obtain comprehensive tissue-specific gene transcri...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303774
更新日期：2020-12-01 00:00:00
abstract：:Nontandem terminal chromosome duplications derived from N. crassa translocation T(I-->VI)NM103 give rise mitotically to some daughter nuclei which have become euploid by loss of one or the other of the two duplicated segments. Loss of the segment in normal sequence occurs as often as loss of the translocated segment. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:It is well known that most new mutations that affect fitness exert deleterious effects and that natural populations are often composed of subpopulations (demes) connected by gene flow. To gain a better understanding of the joint effects of purifying selection and population structure, we focus on a scenario where an a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.178558
更新日期：2015-12-01 00:00:00
abstract：:Previously we have shown that the nucleolus organizer region (NOR) of Neurospora crassa changes size frequently during the premeiotic portion of the sexual phase. Here, we have investigated whether these changes in size originate only in specific regions of the NOR, or are distributed throughout the NOR. In two specia...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Interspecific hybridization in the genus Mus results in male sterility and X-linked placental dysplasia. We have generated several congenic laboratory mouse lines (Mus musculus) in which different parts of the maternal X chromosome were derived from M. spretus. A strict positive correlation between placental weight an...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:We have characterized tandem duplications in the rII regions of phage T4. The rII deletion r1589 blocks only the function of the rIIA cistron, although it extends into the B cistron. Another rII deletion, r1236, blocks the function of the rIIB cistron and overlaps r1589. When a cross is made between r1589 and r1236, t...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:The large-scale systematic variation in nucleotide composition along mammalian and avian genomes has been a focus of the debate between neutralist and selectionist views of molecular evolution. Here we test whether the compositional variation is due to mutation bias using two new tests, which do not assume composition...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303419
更新日期：2020-09-01 00:00:00
abstract：:We present here a molecular analysis of the region surrounding the structural gene encoding alcohol dehydrogenase (Adh) in 47 lines of Drosophila melanogaster that have each accumulated mutations for 300 generations. While these lines show a significant increase in variation of alcohol dehydrogenase enzyme activity co...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:We have precisely mapped and sequenced the three 5' exons of the Drosophila melanogaster Gld gene and have identified the start sites for transcription and translation. The first exon is composed of 335 nucleotides and does not contain any putative translation start codons. The second exon is separated from the first ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:Paramecium has long been a model eukaryote. The sequence of the Paramecium tetraurelia genome reveals a history of three successive whole-genome duplications (WGDs), and the sequences of P. biaurelia and P. sexaurelia suggest that these WGDs are shared by all members of the aurelia species complex. Here, we present th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.163287
更新日期：2014-08-01 00:00:00
abstract：:In the absence of a vg+ gene, extensive cell death occurs in third instar imaginal discs, which results in a complete loss of adult wing margin structures. Essentially all molecularly characterized vg alleles are associated with deletions or insertions of DNA into the vg locus. These alterations reduce or eliminate a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
