The isolation and characterization of Saccharomyces cerevisiae mutants that constitutively express purine biosynthetic genes.

abstract：:mod(mdg4), also known as E(var)3-93D, is involved in a variety of processes, such as gene silencing in position effect variegation (PEV), the control of gypsy insulator sequences, regulation of homeotic gene expression, and programmed cell death. We have isolated a large number of mod(mdg4) cDNAs, representing 21 diff...

journal_title：Genetics

authors： Büchner K,Roth P,Schotta G,Krauss V,Saumweber H,Reuter G,Dorn R

更新日期：2000-05-01 00:00:00

abstract：:Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we...

journal_title：Genetics

authors： Nagarkar S,Wasnik R,Govada P,Cohen S,Shashidhara LS

更新日期：2020-09-01 00:00:00

abstract：:Intrachromosomal gene conversion is the non-reciprocal transfer of information between a pair of repeated genes on a single chromosome. This process produces eventual sequence homogeneity within a family of repeated genes. An evolutionary model for a single chromosome lineage was formulated and analyzed. Expressions w...

journal_title：Genetics

authors： Nagylaki T,Petes TD

更新日期：1982-02-01 00:00:00

abstract：:Meiosis was analyzed cytogenetically in autotetraploids of Arabidopsis, including both established lines and newly generated autotetraploid plants. Fluorescent in situ hybridization with 5S and 45S rDNA probes was used to identify the different chromosomes at metaphase I of meiosis. Multivalents were observed frequent...

journal_title：Genetics

authors： Santos JL,Alfaro D,Sanchez-Moran E,Armstrong SJ,Franklin FC,Jones GH

更新日期：2003-11-01 00:00:00

abstract：:In Saccharomyces cerevisiae, silencers flanking the HML and HMR loci initiate the establishment of transcriptional silencing. We demonstrate that the activity of a silencer pertaining to its potency and directionality is dependent on its genomic position. The context of the HML-E silencer is more permissive to silence...

journal_title：Genetics

authors： Zou Y,Yu Q,Chiu YH,Bi X

更新日期：2006-09-01 00:00:00

abstract：:A study of DNA polymorphism and divergence was conducted for the cytosolic phosphoglucose isomerase (PGI:E.C.5.3.1.9) gene of five species of the mustard genus Leavenworthia: Leavenworthia stylosa, L. alabamica, L. crassa, L. uniflora, and L. torulosa. Sequences of an internal 2.3-kb PgiC gene region spanning exons 6-...

journal_title：Genetics

authors： Filatov DA,Charlesworth D

更新日期：1999-11-01 00:00:00

abstract：:Lethal and sterility mutations were accumulated in a cage population which was initiated with lethal- and sterility-free second chromosomes of D. melanogaster. It took about 2,000 days for the frequencies of these genes to reach equilibrium levels, i.e., 18% lethal and 9% male-sterile chromosomes. Two other cage popul...

journal_title：Genetics

authors： Lee WH,Watanabe TK

更新日期：1977-07-01 00:00:00

abstract：:Among a large number of homologous gene clusters in C. elegans, two gene families that appear to undergo concerted evolution were studied in detail. Both gene families are nematode specific and encode small secreted proteins of unknown function. For both families in three Caenorhabditis species, concerted groups of ge...

journal_title：Genetics

authors： Thomas JH

更新日期：2006-04-01 00:00:00

abstract：:TAHRE, the least abundant of the three retrotransposons forming telomeres in Drosophila melanogaster, has high sequence similarity to the gag gene and untranslated regions of HeT-A, the most abundant telomere-specific retrotransposon. Despite TAHRE's apparent evolutionary relationship to HeT-A, we find TAHRE Gag canno...

journal_title：Genetics

authors： Fuller AM,Cook EG,Kelley KJ,Pardue ML

更新日期：2010-03-01 00:00:00

abstract：:bld2-1 mutant Chlamydomonas reinhardtii strains assemble basal bodies with singlet microtubules; bld2-1 cells display flagellar assembly defects as well as positioning defects of the mitotic spindle and cleavage furrow. To further understand the role of the BLD2 gene, we have isolated three new bld2 alleles and three ...

journal_title：Genetics

authors： Preble AM,Giddings TH Jr,Dutcher SK

更新日期：2001-01-01 00:00:00

abstract：:During animal development, transcription factor activities are modulated by several means, including subcellular localization. The Hox cofactor Extradenticle (Exd) has a dynamic subcellular localization, such that Exd is cytoplasmic by default, but is nuclear when complexed with another homeodomain protein, Homothorax...

journal_title：Genetics

authors： Stevens KE,Mann RS

更新日期：2007-04-01 00:00:00

abstract：:Recombinant inbred lines (RIL) derived from multiple inbred strains can serve as a powerful resource for the genetic dissection of complex traits. The use of such multiple-strain RIL requires a detailed knowledge of the haplotype structure in such lines. Broman (2005) derived the two- and three-point haplotype probabi...

journal_title：Genetics

authors： Teuscher F,Broman KW

更新日期：2007-03-01 00:00:00

abstract：:We examine the efficiency of different genotyping and phenotyping strategies in inbred line crosses from an information perspective. This provides a mathematical framework for the statistical aspects of QTL experimental design, while guiding our intuition. Our central result is a simple formula that quantifies the fra...

journal_title：Genetics

authors： Sen S,Satagopan JM,Churchill GA

更新日期：2005-05-01 00:00:00

abstract：:During the first weeks of human immunodeficiency virus-1 (HIV-1) infection, cytotoxic T-lymphocytes (CTLs) select for multiple escape mutations in the infecting HIV population. In recent years, methods that use escape mutation data to estimate rates of HIV escape have been developed, thereby providing a quantitative f...

journal_title：Genetics

authors： Leviyang S

更新日期：2013-07-01 00:00:00

abstract：:We estimated DNA sequence variation in a 5.7-kb fragment of the furrowed (fw) gene region within and between four populations of Drosophila ananassae; fw is located in a chromosomal region of very low recombination. We analyzed gene flow between these four populations along a latitudinal transect on the Indian subcont...

journal_title：Genetics

authors： Chen Y,Marsh BJ,Stephan W

更新日期：2000-07-01 00:00:00

abstract：:Selection of suppressor mutations that correct growth defects caused by substitutions in an RNA or protein can reveal functionally important molecular structures and interactions in living cells. This approach is particularly useful for the study of complex biological pathways involving many macromolecules, such as pr...

journal_title：Genetics

authors： Brow DA

更新日期：2019-05-01 00:00:00

abstract：:Florida teosinte from Honduras and Guanajuato teosinte have most of their knobs internally located and a common inversion in the short arm of chromosome 8. Teosintes from northern Guatemala, Huixta and Monajil, have all their knobs terminally located and do not have any inversions. Therefore, Florida teosinte from Hon...

journal_title：Genetics

authors： Ting YC

更新日期：1976-08-01 00:00:00

abstract：:We subjected the genes encoding the 19.3-, 21.3c-, and 51-kDa iron-sulfur subunits of respiratory chain complex I from Neurospora crassa to site-directed mutagenesis to mimic mutations in human complex I subunits associated with mitochondrial diseases. The V135M substitution was introduced into the 19.3-kDa cDNA, the ...

journal_title：Genetics

authors： Duarte M,Schulte U,Ushakova AV,Videira A

更新日期：2005-09-01 00:00:00

abstract：:For most common diseases with heritable components, not a single or a few single-nucleotide polymorphisms (SNPs) explain most of the variance for these disorders. Instead, much of the variance may be caused by interactions (epistasis) among multiple SNPs or interactions with environmental conditions. We present a new ...

journal_title：Genetics

authors： Albrechtsen A,Castella S,Andersen G,Hansen T,Pedersen O,Nielsen R

更新日期：2007-06-01 00:00:00

abstract：:Fruit flies of the genus Drosophila have been an attractive and effective genetic model organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them a century ago. Work with Drosophila has enabled dramatic advances in cell and developmental biology, neurobiology and behavior, molecular biology, ...

journal_title：Genetics

authors： Hales KG,Korey CA,Larracuente AM,Roberts DM

更新日期：2015-11-01 00:00:00

abstract：:Additional experiments with homologous as well as heterologous hybridization confirmed our previous finding in Sciara coprophila that XX females have nearly twice the number of ribosomal RNA cistrons as XO males. A comparison between two different X' chromosomes revealed that only the one carrying the irradiation-indu...

journal_title：Genetics

authors： Gerbi SA,Crouse HV

更新日期：1976-05-01 00:00:00

abstract：:Our understanding of the cellular mechanisms by which animals regulate their response to starvation is limited, despite the strong relevance of the problem to major human health issues. The L1 diapause of Caenorhabditis elegans, where first-stage larvae arrest in response to a food-less environment, is an excellent sy...

journal_title：Genetics

authors： Cui M,Wang Y,Cavaleri J,Kelson T,Teng Y,Han M

更新日期：2017-02-01 00:00:00

abstract：:We describe allelic variation at 28 gene loci in natural populations of D. willistoni. Seventy samples were studied from localities extending from Mexico and Florida, through Central America, the West Indies, and tropical South America, down to South Brazil. At least several hundred, and often several thousand, genome...

journal_title：Genetics

authors： Ayala FJ,Powell JR,Tracey ML,Mourão CA,Pérez-Salas S

更新日期：1972-01-01 00:00:00

abstract：:This study attempted to assay the zygotic contribution of X chromosome genes to the genetic control of embryonic morphogenesis in Drosophila melanogaster. A systematic screen for X-linked genes which affect the morphology of the embryo was undertaken, employing the phenotype of whole mount embryos as the major screeni...

journal_title：Genetics

authors： Eberl DF,Hilliker AJ

更新日期：1988-01-01 00:00:00

abstract：:To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors. We found SOL1 that suppresses both point and null LOS1 mutations. Since, when fused to the Ga14p DNA-binding domain, Los1p activates transcription, we tested whether Los1p regulates SOL1. We found that las1 mutants have deplete...

journal_title：Genetics

authors： Shen WC,Stanford DR,Hopper AK

更新日期：1996-06-01 00:00:00

abstract：:The wild-type Salmonella typhimurium strain LT2 contains six copies of the insertion sequence element IS200 which is unique to Salmonella. We have determined the chromosomal locations of all six copies of IS200 in strain LT2. This was done by mapping the positions of Tn10 elements inserted near each copy of IS200. Suc...

journal_title：Genetics

authors： Lam S,Roth JR

更新日期：1983-12-01 00:00:00

abstract：:Populations of several co-existing species of Simocephalus were examined electrophoretically at 11 enzyme loci. The prevailing pattern was one of extensive allelic substitution between species and low levels of polymorphism (0-10%) within species. Genetic identity values between species were very low (10-50%). These r...

journal_title：Genetics

authors： Hann BJ,Hebert PD

更新日期：1982-09-01 00:00:00

abstract：:A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites we...

journal_title：Genetics

authors： Bouza C,Hermida M,Pardo BG,Fernández C,Fortes GG,Castro J,Sánchez L,Presa P,Pérez M,Sanjuán A,de Carlos A,Alvarez-Dios JA,Ezcurra S,Cal RM,Piferrer F,Martínez P

更新日期：2007-12-01 00:00:00

abstract：:Nucleosome-depleted regions (NDRs) are present immediately adjacent to the transcription start site in most eukaryotic promoters. Here we show that NDRs in the upstream promoter region can profoundly affect gene regulation. Chromatin at the yeast HO promoter is highly repressive and numerous coactivators are required ...

journal_title：Genetics

authors： Yarrington RM,Goodrum JM,Stillman DJ

更新日期：2016-02-01 00:00:00

abstract：:Nonrandom associations between alleles at different loci can be tested for using Fisher's exact test. Extensive simulations show that there is a substantial probability of obtaining significant nonrandom associations between closely or completely linked polymorphic neutral loci in a population of constant size at equi...

journal_title：Genetics

authors： Slatkin M

更新日期：1994-05-01 00:00:00