Abstract:
:The distribution of muscle fibres classified on the basis of their content of different myosin heavy chain (MHC) isoforms was analysed in vastus lateralis muscle biopsies of 15 young men (with an average age of 22 y) by correlating immunohistochemistry with specific anti-MHC monoclonal antibodies, myofibrillar ATPase (mATPase) histochemistry and in situ hybridisation with probes specific for MHC beta-slow, MHC-IIA and MHC-IIX. The characterisation of a large number of individual fibres was compared and correlated on a fibre-to-fibre basis. The panel of monoclonal antibodies used in the study allowed classification of human skeletal muscle fibres into 5 categories according to the MHC isoform they express at the protein level, types I, I+IIA, IIA, IIAX and IIX. Hybrid fibres coexpressing two isoforms represented a considerable proportion of the fibre composition (about 14%) and were clearly underestimated by mATPase histochemistry. For a very high percentage of fibres there was a precise correspondence between the MHC protein isoforms and mRNA transcripts. The integrated methods used demonstrate a high degree of precision of the immunohistochemical procedure used for the identification and quantification of human skeletal muscle fibre types. The monoclonal antibody S5-8H2 is particularly useful for identifying hybrid IIAX fibres. This protocol offers new prospects for muscle fibre classification in human experimental studies.
journal_name
J Anatjournal_title
Journal of anatomyauthors
Serrano AL,Pérez M,Lucía A,Chicharro JL,Quiroz-Rothe E,Rivero JLdoi
10.1046/j.1469-7580.2001.19930329.xkeywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
329-37issue
Pt 3eissn
0021-8782issn
1469-7580journal_volume
199pub_type
杂志文章abstract::Since their discovery in different human tissues by Zimmermann in 1898, primary cilia have been found in the vast majority of cell types in vertebrates. Primary cilia are considered to be cellular antennae that occupy an ideal cellular location for the interpretation of information both from the environment and from o...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/joa.12360
更新日期:2015-10-01 00:00:00
abstract::At the tip of the lower beak of the chicken there were found 15-20 specialised dermal papillae containing large numbers of mechanoreceptors. The Merkel or Grandry corpuscles were situated distally in the papillae and at the papillae base was a collection of Herbst corpuscles. The apex of the papillae, under the scanni...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::A vomeronasal organ and patent nasopalatine ducts were found in adult specimens of Aotus trivirgatus, Arctocebus calabarensis, Ateles geoffroyi and Saguinus fuscicollis. The vomeronasal complex appeared to be functional except in Ateles. However, the relative size of the organ in the Platyrrhini is much reduced compar...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::Mammalian pregnancy involves remodelling of the uterine epithelium to enable placentation. In marsupials, such remodelling has probably played a key role in the transition from ancestral invasive placentation to non-invasive placentation. Identifying uterine alterations that are unique to marsupials with non-invasive ...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/joa.13162
更新日期:2020-06-01 00:00:00
abstract::These studies, on a variety of laboratory, zoo and agricultural animals, show that the phenomenon of replacement of tissues lost in an ear punch is common to some other mammals as well as the rabbit, although the rabbit appears to be the most proficient in this process. It is suggested that the cartilage may be replac...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::The gross and microscopic anatomy of the Cape fur seal heart, lung, liver, spleen, stomach, intestine and kidneys (n = 31 seals) is described. Absolute and relative size of organs from 30 male seals are presented, with histological examination conducted on 7 animals. The relationship between log body weight, log organ...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1046/j.1469-7580.1999.19520235.x
更新日期:1999-08-01 00:00:00
abstract::Advances in X-ray computed tomography (CT) have led to a rise in the use of non-destructive imaging methods in comparative anatomy. Among these is contrast-enhanced CT scanning, which employs chemical stains to visualize soft tissues. Specimens may then be 'digitally dissected', producing detailed, three-dimensional d...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/joa.13007
更新日期:2019-08-01 00:00:00
abstract::Complex I is one of the respiratory chain enzymes related to NADH dehydrogenase and is an encoded gene product derived from both nuclear and mitochondrial genomes. Transcription levels of ND1 (mitochondrial) and 51 kDa (nuclear) subunits of complex I in the postnatal development of the intrinsic muscle in rat tongues ...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1046/j.1469-7580.2003.00152.x
更新日期:2003-02-01 00:00:00
abstract::Enzymatic degradation followed by lectin application was carried out on specimens of the rabbit oviduct. The sequential application of the glycosidases (neuraminidase, alpha-L-fucosidase, beta-galactosidase and alpha-mannosidase) and lectins (peanut, winged pea, wheat germ and soybean) seem promising as an effective m...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1985-06-01 00:00:00
abstract::This study analyses the evaluation of tomographic indicators of tibia structure, assuming that the usual loading pattern shifts from uniaxial compression close to the heel to a combined compression, torsion and bending scheme towards the knee. To this end, pQCT scans were obtained at 5% intervals of the tibia length (...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/j.1469-7580.2009.01201.x
更新日期:2010-04-01 00:00:00
abstract::The aim of this study was to record growth-related changes in collagen network organization and proteoglycan distribution in intermittently peak-loaded and continuously lower-level-loaded articular cartilage. Cartilage from the proximal phalangeal bone of the equine metacarpophalangeal joint at birth, at 5, 11 and 18 ...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/j.1469-7580.2009.01140.x
更新日期:2009-11-01 00:00:00
abstract::Degeneration, scar formation and regeneration have been studied after section of the optic nerve in the frog. In the normal optic nerve two types of macroglial cell were identified: astrocytes and oligodendroglia. Degeneration after injury proceeded rapidly in comparison with mammals but did not lead to the production...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::The microvascular anatomy of discrete knee ligaments in adult rabbits is described. Epiligamentous plexuses give rise to a limited number of vessels which penetrate deeply into ligament substance. Intraligamentous vessels are usually longitudinally orientated, widely separated linear anastomoses but occasionally compl...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::A comparison of the relative position of the dorsoventral axes of the bases and heads of the metacarpal bones shows that these do not lie in the same plane, and that torsion can therefore be said to exist in these bones. Torsion is such that the heads of the second and third metacarpals appear to be rotated laterally ...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract::Abstract The skull and the face are directly related, and skeletal morphology influences the facial proportions, muscle structure, feature formation and hence detailed facial appearance. Each face is unique and we are each capable of perceiving very subtle differences between faces in order to carry out personal recog...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/j.1469-7580.2008.00972.x
更新日期:2008-11-27 00:00:00
abstract::In an attempt to clarify the nature of the human cemento-dentinal junction, ground sections of incompletely formed and fully formed extracted teeth were prepared and their histology compared with their microradiographic appearances. The results showed that incompletely formed teeth possess distinctive surface layers o...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::Light and electron microscopic techniques were employed to study the structure of the ureter of the starling (Sturnus vulgaris). Particular attention was paid to the characterisation of the epithelial cell types. Differences were found in the chemical composition of the mucins found in the ureteric epithelial cells an...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potenti...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/j.1469-7580.2007.00719.x
更新日期:2007-05-01 00:00:00
abstract::The backbone or vertebral column is the defining feature of vertebrates and is clearly metameric. Given that vertebrae arise from segmented paraxial mesoderm in the embryo, this metamerism is not surprising. Fate mapping studies in a variety of species have shown that ventromedial sclerotome cells of the differentiate...
journal_title:Journal of anatomy
pub_type: 杂志文章,评审
doi:10.1046/j.1469-7580.2001.19910177.x
更新日期:2001-07-01 00:00:00
abstract::The paraspinal compartment acts as a bone-muscle composite beam of the spine. The elastic properties of the paraspinal muscles play a critical role in spine stabilization. These properties depend on the subjects' posture, and they may be drastically altered by low back pain. Supersonic shear wave elastography can be u...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/joa.12978
更新日期:2019-06-01 00:00:00
abstract::In an adult skull a pre-interparietal bone was found to be present in two symmetrical pieces on either side of the midline. This is the result of failure of fusion of a third pair of centres with each other on the midline. The possibilities of variations due to non-fusion of various pairs of the centres in the interpa...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::The neuromuscular junction (NMJ) is the highly specialised peripheral synapse formed between lower motor neuron terminals and muscle fibres. Post-synaptic acetylcholine receptors (AChRs), which are found in high density in the muscle membrane, bind to acetylcholine released into the synaptic cleft of the NMJ, thereby ...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/joa.13228
更新日期:2020-10-01 00:00:00
abstract::Early hominins formed large and thick-enamelled cheek-teeth within relatively short growth periods as compared with modern humans. To understand better the developmental basis of this process, we measured daily enamel increments, or cross striations, in 17 molars of Plio-Pleistocene hominins representing seven differe...
journal_title:Journal of anatomy
pub_type: 历史文章,杂志文章
doi:10.1111/j.1469-7580.2008.00938.x
更新日期:2008-08-01 00:00:00
abstract::The aim of the study was to evaluate the distribution of apoptosis in the medullary nuclei of infants and adults who died of hypoxic-ischaemic injury. Apoptosis was studied by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) in brainstems from 22 adults (7 subjects who died of opiate into...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/j.1469-7580.2007.00842.x
更新日期:2008-02-01 00:00:00
abstract::The structure of the gonad primordia is described in the embryos of three species of poeciliid fish. Prior to the sexualisation of the germ cells, a dimorphism exists based upon the gonadal somatic features. This serves as a basis for distinguishing between male and female specimens. Presumptive males are characterise...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::The differentiation and patterning of tendon fibroblasts is at present a poorly understood aspect of musculoskeletal development in the vertebrate limb. Precursors of tendon fibroblasts originate in the somatic mesoderm adjacent to the early limb bud and gradually become incorporated into the limb mesenchyme as develo...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1046/j.1469-7580.2003.00145.x
更新日期:2003-01-01 00:00:00
abstract::Retrograde tracers were injected in various parts of the neocortex of the porpoise (Phocoena phocoena). Labelled thalamic neurons were plotted in three-dimensional reconstructions. The lateral geniculate nucleus projects to the visually excitable part of the lateral gyrus. Ventral parts of the medial geniculate nucleu...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Keratinocytes, in response to irritants, secrete pro-inflammatory mediators which recruit and activate immune and mesenchymal cells, including fibroblasts, to repair the skin. Fibroblasts respond by synthesising collagen and promoting the crosslinking extracellular matrix (ECM). We recently showed that the deletion of...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/joa.12442
更新日期:2016-05-01 00:00:00
abstract::Natural loading of the calcanei of deer, elk, sheep and horses produces marked regional differences in prevalent/predominant strain modes: compression in the dorsal cortex, shear in medial-lateral cortices, and tension/shear in the plantar cortex. This consistent non-uniform strain distribution is useful for investiga...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:10.1111/j.1469-7580.2011.01428.x
更新日期:2011-12-01 00:00:00
abstract::Scanning microinterferometric measurements were made of matrix dry mass in the intermediate layer of normal rabbit patellar articular cartilage and in the area surrounding standard, experimental, full-thickness lesions of 1-5 mm diameter. The lesions were created in 1 kg animals and were allowed to heal for up to 3 we...
journal_title:Journal of anatomy
pub_type: 杂志文章
doi:
更新日期:1977-09-01 00:00:00