Abstract:
:One of the first steps in the development of cerebral toxoplasmosis is the penetration of the blood-brain barrier, which is comprised of microvascular endothelial cells. We examined the capacity of human brain microvascular endothelial cells (HBMEC) to interact with Toxoplasma gondii. We found that stimulation of HBMEC with gamma interferon (IFN-gamma) resulted in the induction of toxoplasmostasis. The capacity of HBMEC to restrict Toxoplasma growth after IFN-gamma stimulation was enhanced in the presence of tumor necrosis factor alpha (TNF-alpha). In addition, we found that IFN-gamma induced a strong induction of indoleamine 2,3-dioxygenase (IDO) activity in HBMEC, and this enzyme activity was enhanced by costimulation with TNF-alpha. The addition of excess amounts of tryptophan to the HBMEC cultures resulted in a complete abrogation of the IFN-gamma-TNF-alpha-mediated toxoplasmostasis. We therefore conclude that IDO induction contributed to the antiparasitic effector mechanism inducible in HBMEC by IFN-gamma and TNF-alpha.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Däubener W,Spors B,Hucke C,Adam R,Stins M,Kim KS,Schroten Hdoi
10.1128/IAI.69.10.6527-6531.2001keywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
6527-31issue
10eissn
0019-9567issn
1098-5522journal_volume
69pub_type
杂志文章abstract::Toxin preparations were obtained by growing Clostridium difficile VPI strain 10463 in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM-100 membrane filter. Two toxic activities, designated toxins A and B, were se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1032-1040.1982
更新日期:1982-03-01 00:00:00
abstract::A comparison of viral-induced unresponsiveness of phytohemagglutinin (PHA)-induced deoxyribonucleic acid (DNA) synthesis of mouse lymphocytes was made by culturing the cells under identical conditions in the presence of HEPES buffer in a humid-air atmosphere. The degree of PHA-induced DNA synthesis was found to vary, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.3.438-444.1973
更新日期:1973-03-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.45.3.631-636.1984
更新日期:1984-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.802-805.1982
更新日期:1982-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.253-260.1991
更新日期:1991-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01518-08
更新日期:2009-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2016-09-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2012-06-01 00:00:00
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journal_title:Infection and immunity
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doi:
更新日期:1999-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.629-633.1985
更新日期:1985-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1827-1833.2000
更新日期:2000-04-01 00:00:00
abstract::Randomly bred guinea pigs were infected with Listeria monocytogenes using the intracardial, intravenous and intraperitoneal routes of infection. Doses of Listeria ranged from 5 to 1,000 x the 50% lethal dose based on the 50% lethal dose for intracardially injected Listeria. A complete necropsy was performed on all ani...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.916-924.1977
更新日期:1977-03-01 00:00:00
abstract::The presence of lipopolysaccharide (LPS) in gram-negative bacterial repeats-in-toxin (RTX) toxin preparations, as well as the harsh conditions required to remove it, suggests that LPS may complex with RTX toxins. Concentrated culture supernatant (CCS) preparations of the RTX toxin Pasteurella haemolytica leukotoxin (L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2920-2927.1999
更新日期:1999-06-01 00:00:00
abstract::T lymphocyte subpopulations in the peripheral blood of children with congenital cytomegalovirus infections and in controls were enumerated with monoclonal antibodies. Infants of less than 1 year of age with symptomatic infections showed significant increases in the proportion of suppressor cells and decreases in the r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1380-1382.1983
更新日期:1983-09-01 00:00:00
abstract::Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in sufficient quantity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3536-3541.2001
更新日期:2001-06-01 00:00:00
abstract::In an attempt to understand the mechanism of serum resistance in Actinobacillus pleuropneumoniae, in the present study we examined various interactions among the bacterial surface constituents, serum antibodies, and complement. Analysis of swine sera revealed the presence of anticapsular antibodies in convalescent-pha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3852-3860.1992
更新日期:1992-09-01 00:00:00
abstract::Hepatitis, proliferative typhlitis, and colitis were characterized in young adult and older SCID/NCr mice naturally infected with Helicobacter hepaticus. Liver lesions consisted of Kupffer, Ito, and oval cell hyperplasia along with multifocal to coalescing coagulative hepatocyte necrosis. Numerous Warthin-Starry-posit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5477-5484.1998
更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5166-5172.2001
更新日期:2001-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3637-3640.1999
更新日期:1999-07-01 00:00:00
abstract::Immune complexes have been partially purified from the serum of Plasmodium berghei-infected mice by ultracentrifugation on 10 to 40% linear sucrose gradients, by precipitation with polyethylene glycol, and by gel filtration through Sephacryl S-300. The complexes contain gamma 1, gamma 2a, gamma 2b, and gamma 3 subclas...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.613-618.1978
更新日期:1978-06-01 00:00:00
abstract::In an effort to relate the protein profile to virulence, proteins from the cellular fractions and from culture supernatants of Streptococcus suis capsular type 2 strains from different geographical origins were compared by using Western blots (immunoblots). The protein profiles of the cellular fractions were similar f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1975-1979.1995
更新日期:1995-05-01 00:00:00
abstract::Urinary tract infection (UTI) is the second most common infection in humans, making it a global health priority. Nearly half of all women will experience a symptomatic UTI, with uropathogenic Escherichia coli (UPEC) being the major causative agent of the infection. Although there has been extensive research on UPEC vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-17
更新日期:2018-02-20 00:00:00
abstract::The genetic determinants of the 120-kDa cytotoxin of Actinobacillus pleuropneumoniae serotype 2 were isolated from a lambda DNA library by a plaque immunoblot technique. Expression of the 120-kDa polypeptide was confirmed by Western immunoblot analysis of infected Escherichia coli cell lysates, which were shown to be ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2726-2732.1992
更新日期:1992-07-01 00:00:00
abstract::Monoclonal antibodies to Trichomonas vaginalis were prepared by immunizing mice with a cloned isolate of T. vaginalis. Eight antibodies reacted with the same four isolates or strains but did not react with the other T. vaginalis strains or isolates tested. All eight antibodies reacted uniformly with both the body and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.270-275.1984
更新日期:1984-01-01 00:00:00
abstract::Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Woodchuck sera were tested for antigens and antibodies with tests which detect human hepatitis virus antigens and antibodies. Data on 264 woodchuck sera are presented. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.752-757.1982
更新日期:1982-02-01 00:00:00
abstract::A chimeric recombinant protein composed of the lktA gene product from Pasteurella haemolytica fused to bovine interleukin-2 (IL-2) was made. The LKT-IL-2 chimera was compared with recombinant bovine IL-2 with regard to the ability to induce proliferative responses and LAK cell activity in bovine peripheral blood monon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.565-570.1992
更新日期:1992-02-01 00:00:00
abstract::Escherichia coli 536 (O6:K15:H31), which was isolated from a case of urinary tract infection, determines high nephropathogenicity in a rat pyelonephritis system as measured by renal bacterial counts 7 days after infection. The loss of S fimbrial adhesin formation (Sfa-) (mannose-resistant hemagglutination [Mrh-] and f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.761-767.1986
更新日期:1986-12-01 00:00:00