Abstract：
:DNA double-strand breaks (DSBs) are repaired by homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ in yeast chromosomes has been observed only when HR is blocked, as in rad52 mutants or in the absence of a homologous repair template. We detected yKu70p-dependent imprecise NHEJ at a frequency of approximately 0.1% in HR-competent Rad+ haploid cells. Interestingly, yku70 mutation increased DSB-induced HR between direct repeats by 1.3-fold in a haploid strain and by 1.5-fold in a MAT homozygous (a/a) diploid, but yku70 had no effect on HR in a MAT heterozygous (a/alpha) diploid. yku70 might increase HR because it eliminates the competing precise NHEJ (religation) pathway and/or because yKu70p interferes directly or indirectly with HR. Despite the yku70-dependent increase in a/a cells, HR remained 2-fold lower than in a/alpha cells. Cell survival was also lower in a/a cells and correlated with the reduction in HR. These results indicate that MAT heterozygosity enhances DSB-induced HR by yKu-dependent and -independent mechanisms, with the latter mechanism promoting cell survival. Surprisingly, yku70 strains survived a DSB slightly better than wild type. We propose that this reflects enhanced HR, not by elimination of precise NHEJ since this pathway produces viable products, but by elimination of yKu-dependent interference of HR.
journal_name
Geneticsjournal_title
Geneticsauthors
Clikeman JA,Khalsa GJ,Barton SL,Nickoloff JAkeywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
579-89issue
2eissn
0016-6731issn
1943-2631journal_volume
157pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The genotypes of three color mutants in goldfish: a depigmentation character of larval melanophores, albinism and a recessive-transparent character, were analyzed by crossing experiments. The depigmentation character in the common goldfish is controlled by two dominant multiple genes, Dp1 and Dp2, and only fish with d...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Promoter proximal pausing (PPP) of RNA polymerase II has emerged as a crucial rate-limiting step in the regulation of gene expression. Regulation of PPP is brought about by complexes 7SK snRNP, P-TEFb (Cdk9/cycT), and the negative elongation factor (NELF), which are highly conserved from Drosophila to humans. Here, we...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303419
更新日期：2020-09-01 00:00:00
abstract：:Obligate pathogenic and endosymbiotic bacteria typically experience gene loss due to functional redundancy, asexuality, and genetic drift. We hypothesize that reduced genomes increase their functional complexity through protein multitasking, in which many genes adopt new roles to counteract gene loss. Comparisons of i...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.145656
更新日期：2013-01-01 00:00:00
abstract：:A consensus microsatellite-based linkage map of the turbot (Scophthalmus maximus) was constructed from two unrelated families. The mapping panel was derived from a gynogenetic family of 96 haploid embryos and a biparental diploid family of 85 full-sib progeny with known linkage phase. A total of 242 microsatellites we...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.075416
更新日期：2007-12-01 00:00:00
abstract：:The swi8+ gene of Schizosaccharomyces pombe appears to be involved in the termination step of copy synthesis during mating-type (MT) switching. Mutations in swi8 confer a general mutator phenotype and, in particular, generate specific mutations in the MT region. Sequencing of the MT cassettes of the h90 swi8-137 mutan...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Heterochromatin protein 1a (HP1a) is a highly conserved and versatile epigenetic factor that can both silence and activate transcription. However, the function of HP1a in development has been underinvestigated. Here, we report the role of maternal HP1a in producing maternal transcripts that drive early Drosophila embr...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301704
更新日期：2019-01-01 00:00:00
abstract：:In Saccharomyces cerevisiae, silencers flanking the HML and HMR loci initiate the establishment of transcriptional silencing. We demonstrate that the activity of a silencer pertaining to its potency and directionality is dependent on its genomic position. The context of the HML-E silencer is more permissive to silence...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.055525
更新日期：2006-09-01 00:00:00
abstract：:Recessive mutations in three autosomal genes, him-1, him-5 and him-8, cause high levels of X chromosome nondisjunction in hermaphrodites of Caenorhabditis elegans, with no comparable effect on autosomal disjunction. Each of the mutants has reduced levels of X chromosome recombination, correlating with the increase in ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The S-M checkpoint ensures that entry into mitosis is dependent on completion of DNA replication. In the fission yeast Schizosaccharomyces pombe, the SM checkpoint mutant cdc2-3w is thought to be defective in receiving the checkpoint signal. To isolate genes that function in the checkpoint pathway, we screened an S. p...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:The Saccharomyces cerevisiae nuclear membrane is part of a complex nuclear envelope environment also containing chromatin, integral and peripheral membrane proteins, and large structures such as nuclear pore complexes (NPCs) and the spindle pole body. To study how properties of the nuclear membrane affect nuclear enve...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.119149
更新日期：2010-11-01 00:00:00
abstract：:Predicting the chromosomal location of mapped markers has been difficult because linkage maps do not reveal differences in crossover frequencies along the physical structure of chromosomes. Here we combine a physical crossover map based on the distribution of recombination nodules (RNs) on Solanum lycopersicum (tomato...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.074138
更新日期：2007-08-01 00:00:00
abstract：:Repeated elements are remarkably important for male meiosis and spermiogenesis in Drosophila melanogaster. Pairing of the X and Y chromosomes is mediated by the ribosomal RNA genes of the Y chromosome and X chromosome heterochromatin, spermiogenesis depends on the fertility factors of the Y chromosome. Intriguingly, a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:We propose a new method for calculating probabilities for pedigree genetic data that incorporates crossover interference using the chi-square models. Applications include relationship inference, genetic map construction, and linkage analysis. The method is based on importance sampling of unobserved inheritance pattern...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：:Most species of the Drosophila genus and other Diptera are polymorphic for paracentric inversions. A common observation is that successful inversions are of intermediate size. We test here the hypothesis that the selected property is the recombination length of inversions, not their physical length. If so, physical le...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:The formation and stability of epigenetically regulated chromatin is influenced by DNA replication and factors that modulate post-translational modifications on histones. Here we describe evidence that PCNA can affect silencing in Saccharomyces cerevisiae by facilitating deposition of H3 K56ac onto chromosomes. We pro...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.084525
更新日期：2008-06-01 00:00:00
abstract：:Microtubules are dynamic structures that must elongate, disassemble, and be cleaved into smaller pieces for proper neuronal development and function. The AAA ATPase Spastin severs microtubules along their lengths and is thought to regulate the balance between long, stable filaments and shorter fragments that seed exte...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.130831
更新日期：2011-09-01 00:00:00
abstract：:Differentiation in morphometric traits among experimental populations of the housefly subjected to an experimental bottleneck was examined for replicate lines founded with one, four or 16 pairs of flies. Differentiation among lines within a bottleneck size was significantly greater than predicted by drift in relation ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:According to theory, F(2) hybrid breakdown (lethality or sterility) is due to incompatibilities between interacting genes of the different species (i.e., the breaking up of coadapted gene complexes). Detection of such incompatibilities is particularly straightforward in haplodiploid species, because virgin F(1) hybrid...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:The genetic effects of one generation of spermatogonial X-irradiation in rats, by a single dose of 600r in one experiment and by a fractionated dose of 450r in another, were measured in three generations of their descendants. Estimates of dominant lethal mutation rates--(2 to 3) X 10-4/gamete/r--from litter size diffe...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:The polarized partitioning of proteins in cells underlies asymmetric cell division, which is an important driver of development and cellular diversity. The budding yeast Saccharomyces cerevisiae divides asymmetrically, like many other cells, to generate two distinct progeny cells. A well-known example of an asymmetric...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303439
更新日期：2020-11-01 00:00:00
abstract：:The central and marginal populations of D. robusta differ greatly in the level of inversion polymorphism; the marginal populations are monomorphic or nearly so and the central populations are highly polymorphic. This paper presents the frequencies of alleles at forty gene loci in various populations of D. robusta, stu...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-10-01 00:00:00
abstract：:Crossovers (COs) generated through meiotic recombination are important for the correct segregation of homologous chromosomes during meiosis. Several models describing the molecular mechanism of meiotic recombination have been proposed. These models differ in the arrangement of heteroduplex DNA (hDNA) in recombination ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.070367
更新日期：2007-05-01 00:00:00
abstract：:The gilthead sea bream (Sparus aurata L.) is a marine fish of great importance for fisheries and aquaculture. It has also a peculiar sex-determination system, being a protandrous hermaphrodite. Here we report the construction of a first-generation genetic linkage map for S. aurata, based on 204 microsatellite markers....
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.059014
更新日期：2006-10-01 00:00:00
abstract：:Specific mutations in the gene encoding the largest subunit of RNA polymerase II (RpII215) cause a partial transformation of a structure of the third thoracic segment, the capitellum, into the analogous structure of the second thoracic segment, the wing. This mutant phenotype is also caused by genetically reducing the...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:We have used the results of an experiment mapping quantitative trait loci (QTL) affecting milk yield and composition to estimate the total number of QTL affecting these traits. We did this by estimating the number of segregating QTL within a half-sib daughter design using logic similar to that used to estimate the "fa...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.077784
更新日期：2007-10-01 00:00:00
abstract：:The murine dilute suppressor gene (dsu) suppresses the coat-color phenotype of three pigment mutations, dilute (d), ashen (ash) and leaden (ln), that each produce adendritic melanocytes. Suppression is due to the ability of dsu to partially restore (ash and ln), or almost completely restore (d), normal melanocyte morp...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Crossovers (COs) between homologous chromosomes are critical for meiotic chromosome segregation and form in the context of the synaptonemal complex (SC), a meiosis-specific structure that assembles between aligned homologs. During Caenorhabditis elegans meiosis, central region components of the SC (SYP proteins) are e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302625
更新日期：2019-10-01 00:00:00
abstract：:A likelihood ratio test is presented for comparing rates of evolutionary change in the paths of descent leading to two species. The test is compared to previous relative rate tests based on variances of estimated numbers of base substitutions. The likelihood approach allows for different transversion and transition ra...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Mitomycin C (MMC) is a genotoxic agent that induces DNA cross-links, DNA alkylation, and the production of reactive oxygen species (ROS). MMC induces the SOS response and RpoS regulons in Escherichia coli SOS-encoded functions are required for DNA repair, whereas the RpoS regulon is typically induced by metabolic stre...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.199471
更新日期：2017-07-01 00:00:00
abstract：:The Drosophila piwi gene is the founding member of the only known family of genes whose function in stem cell maintenance is highly conserved in both animal and plant kingdoms. piwi mutants fail to maintain germline stem cells in both male and female gonads. The identification of piwi-interacting genes is essential fo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
