Abstract:
:Epidemiological studies have linked consumption of broccoli to a reduced risk of colon cancer in individuals with the glutathione S-transferase M1 (GSTM1) null genotype. GSTs are involved in excretion and elimination of isothiocyanates (ITCs), which are major constituents of broccoli and other cruciferous vegetables and have cancer chemopreventive potential, so it is speculated that ITCs may play a role in protection against human colon cancer. However, there is a lack of data from animal studies to support this. We carried out a bioassay to examine whether sulforaphane (SFN) and phenethyl isothiocyanate (PEITC), major ITCs in broccoli and watercress, respectively, and their corresponding N:-acetylcysteine (NAC) conjugates, show any chemopreventive activity towards azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) in F344 rats. Groups of six male F344 rats were treated with AOM subcutaneously (15 mg/kg body wt) once weekly for 2 weeks. SFN and PEITC and their NAC conjugates were administered by gavage either three times weekly for 8 weeks (5 and 20 micromol, respectively) after AOM dosing (post-initiation stage) or once daily for 3 days (20 and 50 micromol, respectively) before AOM treatment (initiation stage). The bioassay was terminated on week 10 after the second AOM dosing and ACF were quantified. SFN, SFN-NAC, PEITC and PEITC-NAC all significantly reduced the formation of total ACF from 153 to 100-116 (P < 0.01) and multicrypt foci from 52 to 27-38 (more than four crypts/focus; P < 0.05) during the post-initiation treatment. However, only SFN and PEITC were effective during the initiation phase, reducing the total ACF from 153 to 109-115 (P < 0.01) and multicrypt foci from 52 to 35 (more than four crypts/focus; P < 0.05). The NAC conjugates were inactive as anti-initiators against AOM-induced ACF. These findings provide important laboratory evidence for a potential role of SFN and PEITC in the protection against colon cancer.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Chung FL,Conaway CC,Rao CV,Reddy BSdoi
10.1093/carcin/21.12.2287keywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
2287-91issue
12eissn
0143-3334issn
1460-2180journal_volume
21pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Microsatellite instability (MSI) is present in ulcerative colitis (UC) and colitis-associated colorectal cancers (CAC). Certain factors released by polymorphonuclear cells (PMNs) may drive mucosal frameshift mutations resulting in MSI and cancer. Here, we applied a co-culture system with PMNs and colon epithelial cell...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgx118
更新日期:2018-02-09 00:00:00
abstract::Previous studies have indicated that the Nb rat has a higher predisposition to the spontaneous development of nephroblastoma than most other strains of laboratory rat. In order to determine whether this inherent susceptibility could be exploited as a model for Wilms' tumor, Nb rats were treated with various chemicals ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.11.1551
更新日期:1985-11-01 00:00:00
abstract::One of the peaks present in HPLC profiles of [3H]benzo[a]-pyrene (BaP)-deoxyribonucleosides prepared by enzymatic degradation of [3H]BaP-DNA isolated from Wistar rat embryo cell cultures exposed to [G-3H]BaP was found to be r-7,c-9,c-10,t-8-tetrahydroxy-7,8,9,10-tetrahydroBaP, a BaP-DNA adduct decomposition product (P...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.10.1863
更新日期:1988-10-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is an important process implicated in tumor invasion and metastasis. Twist1 is a transcription factor that induces EMT, including E-cadherin suppression and cancer cell migration and invasion; hence it promotes cancer metastasis. Twist1 directly or indirectly regulates the expre...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgv005
更新日期:2015-03-01 00:00:00
abstract::The N-hydroxylamine of the carcinogen 2-amino-3-methylimidazolo[4,5-f]quinoline (IQ) covalently bound to calf thymus DNA at pH 7, and the binding was 11% higher under acidic conditions (pH 5). The extent of N-hydroxy-IQ binding to single-stranded polynucleotides at neutral pH was in the following order: polyguanylic a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.6.1061
更新日期:1988-06-01 00:00:00
abstract::4,4'-Methylenediphenyl diisocyanate (MDI) is the most widely used isocyanate in the manufacture of polyurethanes. MDI has been implicated as one of the major causes of occupational asthma. Hydrolysis of MDI can yield 4,4'-methylenedianiline (MDA), which is a suspected human carcinogen. Thus the need to monitor occupat...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.10.2583
更新日期:1995-10-01 00:00:00
abstract::Epidemiological studies have shown that a high incidence of oral cancers is associated with chewing betel quid. Since chromosomal aberrations are involved in many types of cancers, we investigated whether increased frequencies of chromosomal alterations could be detected in the oral mucosa cells of betel quid chewers ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.12.2347
更新日期:1997-12-01 00:00:00
abstract::The breast cancer gene BRCA1 has previously been cloned from both human and mouse. We cloned a fragment of the rat Brca1 homologue in order to map it and explore its biological function. Partial cDNA fragments of the rat Brca1 homologue were isolated by RT-PCR. Sequence analysis revealed that the RING-finger domain is...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.8.1561
更新日期:1996-08-01 00:00:00
abstract::Epidemiologic studies have suggested that aromatic amines (and nitroaromatic hydrocarbons) may be carcinogenic for human pancreas. Pancreatic tissues from 29 organ donors (13 smokers, 16 non-smokers) were examined for their ability to metabolize aromatic amines and other carcinogens. Microsomes showed no activity for ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.5.1085
更新日期:1997-05-01 00:00:00
abstract::The association between prostate cancer risk and dietary fat consumption is well documented and explained partly by accelerated lipid peroxidation. We explored the possible effects of high dietary cholesterol on carcinogenesis and oxidative stress in the prostate of ACI/Seg rats. The rats develop prostate cancer spont...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh105
更新日期:2004-06-01 00:00:00
abstract::Teleocidin, a new tumor promoter, increased incorporation of radioactive inorganic phosphate (32Pi) into phospholipids in HeLa cells. This effect was detected within 1 h on incubation of the cells in medium containing teleocidin. The half-maximum effective dose of teleocidin was approximately 10 ng/ml. The main effect...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.1.107
更新日期:1983-01-01 00:00:00
abstract::Inactivation of the adenomatous polyposis coli (Apc) gene by loss of the wild-type Apc allele (LOH) is a prerequisite for the development of intestinal adenomas in Msh2 proficient Min (Apc+/-Msh2+/+) mice. In contrast, adenomas from Msh2 deficient Min (Apc+/-Msh2-/-) mice are not usually associated with LOH. Given the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.2.217
更新日期:2003-02-01 00:00:00
abstract::Transforming growth factor-alpha (TGF-alpha) is a potent mitogenic factor which acts by binding to the epidermal growth factor (EGF). c-erbB-2 is a member of the EGF receptor family and is known to be associated with cellular growth and differentiation. The roles played by these factors in benign prostatic hyperplasia...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.4.729
更新日期:1995-04-01 00:00:00
abstract::Multidrug resistance 2 (Mdr2), also called adenosine triphosphate-binding cassette B4 (ABCB4), is the transporter of phosphatidylcholine (PC) at the canalicular membrane of mouse hepatocytes, which plays an essential role for bile formation. Mutations in human homologue MDR3 are associated with several liver diseases....
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgv156
更新日期:2016-01-01 00:00:00
abstract::To elucidate the mechanism of metabolic activation of 3'-methyl-N,N-dimethyl-4-aminoazobenzene (3'-Me-DAB), the mutagenicity of its metabolites and some related azo compounds towards Salmonella typhimurium TA-98 and TA-100 was investigated. Mutagenicity of the metabolites, either N-demethylated or oxidized at the 3'-m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.2.121
更新日期:1980-02-01 00:00:00
abstract::Diethylstilbestrol (DES), a synthetic stilbene estrogen, is a potent development toxin and carcinogen in humans and rodents. A number of 32P-postlabeling studies suggest that genotoxic effects of DES substantially contribute to these biological effects. The mechanisms involved in DES-mediated genotoxicity are not comp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.11.2643
更新日期:1995-11-01 00:00:00
abstract::A line of C3H mouse fibroblasts designated CVP3SC6 (CVP) was found to be nonresponsive to the induction of morphological transformation by the polycyclic aromatic hydrocarbon benzo[a]pyrene (BaP) and its BaP-7,8-diol and BaP-diol-epoxide derivates. This cell line was, however, susceptible to BaP-induced mutation to ou...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.9.963
更新日期:1982-01-01 00:00:00
abstract::Reduced retinyl ester synthesis has been associated with several forms of cancer; we therefore proposed studying melanoma development from the perspective of this biochemical pathway. Cultures of human melanoma cells with fibroblastoid morphology showed negligible retinyl ester synthesis; in sharp contrast, those with...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.11.1821
更新日期:2002-11-01 00:00:00
abstract::Forty-two compounds belonging to various chemical groups have been tested for their ability to suppress formation of aflatoxin B1--DNA adduct mediated by microsome in vitro. While many of these compounds have either marginal or no modulating effect, some have been identified as effective inhibitors. The strong inhibit...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.10.1359
更新日期:1984-10-01 00:00:00
abstract::Currently there is no well-defined biological parameter or marker to help define agents, doses, and dose schedules for human cancer chemoprevention trials. Induction of ornithine decarboxylase, the rate limiting enzyme in the polyamine biosynthetic pathway, has been shown to be an essential aspect of mouse skin tumor ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.11.1589
更新日期:1985-11-01 00:00:00
abstract::Chondrosarcoma is a form of malignant skeletal tumor of cartilaginous origin. The non-malignant form of the disease is termed chondroma. Correctly distinguishing between the two forms is essential for making therapeutic decisions. However, due to their similar histological appearances and the lack of a reliable diagno...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs254
更新日期:2012-11-01 00:00:00
abstract::Information suggests that the cyclooxygenase (COX) metabolites, the prostanoids, play a role in gall bladder physiology and disease. Non-steroidal anti-inflammatory drugs which inhibit COX enzymes have been shown in vivo and in vitro to alter the growth patterns of intestinal epithelial cells, and specific COX-2 inhib...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::The experiments reported in this study were designed to examine the question of whether a mammary epithelial cell's independence from hormonal requirements is established at the time of carcinogenic initiation, or whether the emergence of hormone independence is associated with the process of tumor progression. A newl...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.3.383
更新日期:1998-03-01 00:00:00
abstract::Carnosol is a naturally occurring phytopolyphenol found in rosemary. Carnosol functions as antioxidant and anticarcinogen. In the present study, we compared the antioxidant activity of carnosol and other compounds extracted from rosemary. Carnosol showed potent antioxidative activity in alpha,alpha-diphenyl-beta-picry...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.6.983
更新日期:2002-06-01 00:00:00
abstract::The metabolic activation of the heterocyclic food carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) by two human cytochrome P450 monoxygenases (P4501A1 and P4501A2) and two human N-acetyltransferases (NAT1 and NAT2) was investigated. Various combinations of these enzymes were functionally expressed in COS-1 cell...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.10.1713
更新日期:1992-10-01 00:00:00
abstract::One hundred forty-seven female Sprague-Dawley rats were divided into 5 groups and at 60 days of age were treated i.g. with 5 mg of 7,12-dimethylbenzanthracene (DMBA) suspended in 1.0 ml of sesame oil. Selenium (Se), as selenium dioxide (SeO2), was administered in the drinking water to 4 of the 5 groups (30 rats/group)...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.6.519
更新日期:1981-01-01 00:00:00
abstract::The rates of reaction and the products formed when two vicinal diol-epoxides derived from benz(a)anthracene, anti-BA-3,4-diol 1,2-oxide (t-3,r-4-dihydroxy-t-1,2-oxy-1,2,3,4-tetrahydrobenz(a) anthracene)* and anti-BA-8,9-diol 10,11-oxide (r-8,t-9-dihydroxy-t-10,11-oxy-8,9,10,11-tetrahydrobenz(a)anthracene) reacted with...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/1.3.277
更新日期:1980-03-01 00:00:00
abstract::There is indirect evidence to suggest that the excision-repair mechanism responsible for the removal of UV-induced thymine dimers may also play a role in the repair of cis-diamminedichloroplatinum(II) (cis-DDP)-induced DNA adducts in both bacteria and mammalian cells. It was hypothesized that UV dimers and cis-DDP add...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.8.1465
更新日期:1989-08-01 00:00:00
abstract::An important difference between chemical agents that induce oxidative damage in DNA and ionizing radiation is that radiation-induced damage is clustered locally on the DNA. Both modelling and experimental studies have predicted the importance of clustering of lesions induced by ionizing radiation and its dependence on...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.5.905
更新日期:1999-05-01 00:00:00
abstract::We investigated possible mechanisms associated with promotion of hepatocarcinogenesis by the synthetic estrogens mestranol (M) and ethinyl estradiol (EE). Our first objective was to determine whether chronic EE treatment was associated with hepatotoxicity accompanied by regenerative hyperplasia. Female Sprague-Dawley ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.12.2007
更新日期:1986-12-01 00:00:00