Abstract:
:Carnosol is a naturally occurring phytopolyphenol found in rosemary. Carnosol functions as antioxidant and anticarcinogen. In the present study, we compared the antioxidant activity of carnosol and other compounds extracted from rosemary. Carnosol showed potent antioxidative activity in alpha,alpha-diphenyl-beta-picrylhydrazyl (DPPH) free radicals scavenge and DNA protection from Fenton reaction. High concentrations of nitric oxide (NO) are produced by inducible NO synthase (iNOS) in inflammation and multiple stages of carcinogenesis. Treatment of mouse macrophage RAW 264.7 cell line with carnosol markly reduced lipopolysaccharide (LPS)-stimulated NO production in a concentration-related manner with an IC50 of 9.4 microM; but other tested compounds had slight effects. Western blot, reverse transcription-polymerase chain reaction, and northern blot analyses demonstrated that carnosol decreased LPS-induced iNOS mRNA and protein expression. Carnosol treatment showed reduction of nuclear factor-kappaB (NF-kappaB) subunits translocation and NF-kappaB DNA binding activity in activated macrophages. Carnosol also showed inhibition of iNOS and NF-kappaB promoter activity in transient transfection assay. These activities were referred to down-regulation of inhibitor kappaB (IkappaB) kinase (IKK) activity by carnosol (5 microM), thus inhibited LPS-induced phosphorylation as well as degradation of IkappaBalpha. Carnosol also inhibited LPS-induced p38 and p44/42 mitogen-activated protein kinase (MAPK) activation at a higher concentration (20 microM). These results suggest that carnosol suppresses the NO production and iNOS gene expression by inhibiting NF-kappaB activation, and provide possible mechanisms for its anti-inflammatory and chemopreventive action.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Lo AH,Liang YC,Lin-Shiau SY,Ho CT,Lin JKdoi
10.1093/carcin/23.6.983keywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
983-91issue
6eissn
0143-3334issn
1460-2180journal_volume
23pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::When exogenous [(3)H]retinol (vitamin A) was added to culture medium, normal human epithelial cells from the oral cavity, skin, lung and breast took up and esterified essentially all of the [(3)H]retinol within a few hours. As shown by [(3)H]retinol pulse-chase experiments, normal epithelial cells then slowly hydrolyz...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.11.1925
更新日期:2000-11-01 00:00:00
abstract::Glucosinolates are common components of cruciferous vegetables that can be hydrolyzed during food processing to yield isothiocyanates, some of which have been shown to inhibit the induction of mammary tumors in rats by 7,12-dimethyl-benz[a]anthracene. To determine how intact glucosinolates affect the metabolism of ben...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.4.657
更新日期:1988-04-01 00:00:00
abstract::Cancer of the stomach is one of the most commonly diagnosed malignancies and remains an important cause of mortality world wide. This type of cancer is not uniformly distributed among populations but shows a marked variation in both incidence and mortality. Although gastric cancer is declining in many parts of the wor...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/20.12.2195
更新日期:1999-12-01 00:00:00
abstract::Glucuronide conjugates of 4-aminobiphenyl and its N-hydroxy metabolite can be rapidly hydrolyzed in acidic urine to undergo further metabolic activation and form DNA adducts in the urothelium. We conducted a large multicenter case-control study in Spain to explore the etiology of bladder cancer and evaluated the assoc...
journal_title:Carcinogenesis
pub_type: 杂志文章,多中心研究
doi:10.1093/carcin/bgr048
更新日期:2011-06-01 00:00:00
abstract::N-Nitrosodimethylamine (NDMA) is a potent mutagen and animal carcinogen to which many people are exposed through the consumption of contaminated food and the use of tobacco products. Although the mutational specificity of NDMA has been studied in bacteria, little is known about the specific types of mutations induced ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.5.755
更新日期:1998-05-01 00:00:00
abstract::p53-upregulated modulator of apoptosis (PUMA) plays an essential role in p53-dependent apoptosis following DNA damage. PUMA also mediates apoptosis independent of p53. In this study, we investigated the role and mechanism of PUMA induction in response to serum starvation in p53-deficient cancer cells. Following serum ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn150
更新日期:2008-10-01 00:00:00
abstract::Metabolism and nucleic acid binding of the mammary gland carcinogen N-hydroxy-4-acetylaminobiphenyl (N-OH-AABP) was investigated using the human mammary epithelial cell line MCF 10A. Chromatographic analysis of the ethyl acetate extract of the media from cultured MCF 10A after 24 h exposure to N-OH-AABP revealed the f...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.4.611
更新日期:1994-04-01 00:00:00
abstract::The use of naturally occurring botanicals with substantial antioxidant activity to afford protection to human skin against UV damage is receiving increasing attention. The green tea constituent (-)-epigallocatechin-3-gallate (EGCG) is a potent antioxidant and has shown remarkable preventive effects against photocarcin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.2.287
更新日期:2001-02-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the leading causes for cancer related mortality worldwide. Poor prognosis of HCC patients is mainly due to frequent metastasis and recurrence. Deregulation of metastasis suppressors in malignant cells plays critical roles during cancer metastasis. Thus, novel metastasis suppres...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgv108
更新日期:2015-10-01 00:00:00
abstract::Hypoxia-inducible factors (HIFs), in particular HIF-1alpha, have been implicated in tumor biology. However, HIF target genes in the esophageal tumor microenvironment remain elusive. Gene expression profiling was performed upon hypoxia-exposed non-transformed immortalized human esophageal epithelial cells, EPC2-hTERT, ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp326
更新日期:2010-03-01 00:00:00
abstract::Folate and vitamin D have been shown to be influenced by ultraviolet (UV) radiation. UVA radiation can break down plasma folate, whereas vitamin D can be synthesized in UVB-exposed skin. Folate metabolism is involved in DNA synthesis and repair, and vitamin D processes anti-proliferative effects. The functions of both...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl156
更新日期:2007-02-01 00:00:00
abstract::Crude plasma membranes were prepared from the liver of control rats or of rats submitted to an initiation by diethyl-nitrosamine and selection with 2-acetylaminofluorene and carbon tetrachloride (group IS) or of rats submitted to an initiation-selection protocol followed by a promotion with phenobarbital (group IS PB)...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/12.4.659
更新日期:1991-04-01 00:00:00
abstract::To determine whether B or T lymphoid malignancies could be induced following the exposure of lymphoid cells within different lymphoid organs to a potential chemical carcinogen, 3-methylcholanthrene (3-MC) was directly injected into surgically exposed Peyer's patches (PP), mesenteric lymph nodes (MLN), axillary lymph n...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.8.1257
更新日期:1986-08-01 00:00:00
abstract::Lung tumors were induced in A/J mice by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and the related compounds acetoxymethylmethylnitrosamine (AMMN) and 4-acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone (NNKOAc). NNK both methylates and pyridyloxobutylates DNA while AMMN and...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.11.2419
更新日期:1993-11-01 00:00:00
abstract::When hepatocytes isolated from mouse liver are cultivated in vitro, a small fraction of cells can survive, forming colonies, while most cells die within a few weeks. We compared the colony forming capacity of hepatocytes in three mouse strains; two strains susceptible for hepatocarcinogenesis, C3H/HeJ (C3) and DBA/2J ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.6.1103
更新日期:1998-06-01 00:00:00
abstract::Adipocytokines are a group of adipocyte-secreted proteins that have significant effects on the metabolism of lipids and carbohydrates, as well as numerous other processes. A number of recent studies have indicated that some adipocytokines may significantly influence the proliferation of malignant cells in vitro, where...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn080
更新日期:2008-05-01 00:00:00
abstract::1,3-Butadiene (BD) is an indirect alkylating agent that has greater cancer potency in the mouse than in the rat. The purpose of the present study was to compare the mutagenic potency of BD at the hprt locus of T-lymphocytes of exposed mice and rats and to determine whether mutations induced in this marker gene can be ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.6.1019
更新日期:1998-06-01 00:00:00
abstract::[3H]Benzo[a]pyrene (B[a]P) was administered to male Sprague-Dawley rats via intratracheal instillation, and bile was collected over a period of 6 h. Conjugated metabolites of B[a]P in bile were separated by paper chromatography or reversed-phase ion-pair HPLC and quantified by liquid scintillation spectrometry. In pap...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.3.403
更新日期:1992-03-01 00:00:00
abstract::Circulating microRNAs (miRNAs) are potential biomarkers for cancer diagnosis, screening and prognosis. This study aimed to identify serum miRNAs as predictors of survival in patients with advanced non-small cell lung cancer (NSCLC). We profiled serum miRNAs in a pilot set of four patients with good survival (>24 month...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy132
更新日期:2019-07-04 00:00:00
abstract::In order to explore a possibility that the custom of drinking green tea infusion is efficacious for reducing the carcinogenic risk of environmental exposure to pentachlorophenol (PCP), we examined the effects in a hepato- and cholangiocarcinogenesis model in mice exposed to diethylnitrosamine (DEN). In the first exper...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg053
更新日期:2003-06-01 00:00:00
abstract::Progress in prostate cancer research has been hindered by the lack of well characterized, immortalized, human prostatic epithelial cell lines that express markers of normal prostatic epithelial cells and mimic normal growth and differentiation responses to androgens. The objectives of this study were to: (i) establish...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.8.1641
更新日期:1996-08-01 00:00:00
abstract::Currently there is no well-defined biological parameter or marker to help define agents, doses, and dose schedules for human cancer chemoprevention trials. Induction of ornithine decarboxylase, the rate limiting enzyme in the polyamine biosynthetic pathway, has been shown to be an essential aspect of mouse skin tumor ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.11.1589
更新日期:1985-11-01 00:00:00
abstract::Stimulation of cell proliferation caused by peroxisome proliferators was blocked by antibodies against TNF alpha and agents that inactivate Kupffer cells, a rich source of TNF alpha, which supports the hypothesis that Kupffer cells play a pivotal role in peroxisome proliferator-induced hyperplasia. Here, the ability o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.7.1217
更新日期:1998-07-01 00:00:00
abstract::Food-derived aminoimidazoazarenes have been shown to be mutagenic and carcinogenic and to form covalent DNA adducts. 32P-Post-labelling analysis of DNA modified with these heterocyclic amines (HA), including 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.5.877
更新日期:1994-05-01 00:00:00
abstract::The O6-methylguanine-DNA methyltransferase gene (MGMT) encodes the direct reversal DNA repair protein that removes alkyl adducts from the O6 position of guanine. Several single-nucleotide polymorphisms (SNPs) exist in the MGMT promoter/enhancer (P/E) region. However, the haplotype structure encompassing these SNPs and...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt355
更新日期:2014-03-01 00:00:00
abstract::Examination of p53 mutation spectra may provide clues to molecular mechanisms involved in different histologic types of gastric cancer. A total of 105 gastric cancer cases classified according to the Laurén's system were selected from a high-risk area around Florence, Italy. Exons 5-8 of the p53 gene were examined for...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.12.2145
更新日期:1998-12-01 00:00:00
abstract::Cryptic deletions at chromosome 6q are common cytogenetic abnormalities in T-cell lymphoblastic leukemia/lymphoma (T-LBL), but the target genes have not been formally identified. Our results build on detection of specific chromosomal losses in a mouse model of γ-radiation-induced T-LBLs and provide interesting clues f...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr271
更新日期:2012-02-01 00:00:00
abstract::The role of rat O6-alkylguanine-DNA alkyltransferase (AGT) in modulating mutagenesis by O6-substituted guanines in the rat H-ras gene was examined. Rat4 cells were transfected with vectors carrying O6-methyl-, O6-ethyl- or O6-benzylguanine residues in place of the normal guanines at either the first, second, or both t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.4.593
更新日期:1993-04-01 00:00:00
abstract::Thioredoxin reductases (TrxRs) catalyse the NADPH-dependent reduction of thioredoxin and play an important role in multiple cellular events related to carcinogenesis including cell proliferation, apoptosis and cell signaling. We have used human hepatoma HepG2 cells to examine the regulation of TrxRs by isothiocyanate ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.3.497
更新日期:2003-03-01 00:00:00
abstract::Spices and flavoring plants part rich in supposedly health-promoting phytochemicals are currently receiving much attention as a possible source of cancer chemopreventive compounds. Clove, the sun-dried unopened flower bud from the plant Syzygium aromaticum L. is a commonly used spice and food flavor. In the present wo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi372
更新日期:2006-08-01 00:00:00