Abstract：
:Adeno-associated virus (AAV) vectors were shown capable of high efficiency transduction of both dividing and nondividing cells and tissues. AAV-mediated transduction leads to stable, long-term transgene expression in the absence of apparent immune response. These properties and the broad host range of AAV vectors indicate that they constitute a powerful tool for gene therapy purposes. An additional potential benefit of AAV vectors is their ability to integrate site-specifically in the presence of Rep proteins which can be expressed transiently, thus limiting their suspected adverse effects. The major restrictions of AAV as vectors are their limited genetic capacity and strict packaging size constraint of less than 5 kb. Another difficulty is the labor-intensive and expensive procedure for the production and packaging of recombinant AAV vectors. The major benefits and drawbacks of AAV vectors and advances made in the past 3 years are discussed.
journal_name
J Biomed Scijournal_title
Journal of biomedical scienceauthors
Tal Jdoi
10.1007/BF02253246keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
279-91issue
4eissn
1021-7770issn
1423-0127pii
25461journal_volume
7pub_type
杂志文章,评审abstract：BACKGROUND:Endothelial cell (EC) dysfunctions, including turnover enrichment, gap junction disruption, inflammation, and oxidation, play vital roles in the initiation of vascular disorders and atherosclerosis. Hemodynamic forces, i.e., atherprotective pulsatile (PS) and pro-atherogenic oscillatory shear stress (OS), ca...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-019-0551-8
更新日期：2019-08-07 00:00:00
abstract：BACKGROUND:Promoting post-stroke neurogenesis has long been proposed to be a therapeutic strategy for the enhancement of functional recovery after cerebral ischemic stroke. Despite numerous approaches have been widely reported the proliferation or differentiation of the neurogenic population therapeutic strategies by t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0521-1
更新日期：2019-04-18 00:00:00
abstract：BACKGROUND:Human papillomavirus (HPV) is the most common sexually transmitted virus in women worldwide. The persistence of the virus may cause warts that are considered benign lesions and low or high grade intraepithelial lesions (LSIL/HSIL). Immunological system plays an important role in the resolution of infections....
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0472-y
更新日期：2018-09-18 00:00:00
abstract：BACKGROUND:Prevalence of metabolic syndrome (MS) is rapidly increasing worldwide. To investigate the spread of MS risks and its relationship with eating habits including fish intake, we carried out a health examination for young and middle-aged men. METHODS:The subjects were 97 healthy men (20 to 50 years) living in M...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-S1-S34
更新日期：2010-08-24 00:00:00
abstract：:The dual signal hypothesis of apoptosis holds that a common signal can activate both apoptotic and proliferative pathways. The fate of a cell is dependent on which of these two pathways predominates. In the MAPK family of kinases, ERK and JNK have been proposed to mediate apoptosis whereas the PI3K-stimulated kinase, ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255905
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND:Oxidized low-density lipoprotein (oxLDL) mediates the transformation of macrophages (MΦ) to cholesterol-rich foam cells and the release of pro-inflammatory cytokines during atherogenesis. JAB1 (Jun activation domain binding protein-1) is present in all stages of human plaques, involved in the Toll-like recep...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-017-0320-5
更新日期：2017-02-07 00:00:00
abstract：:Mediation of the respiratory reflex effects of an exogenous serotonin challenge goes beyond the lung vagi and is suggested to involve the nodose ganglia. In the present experiments the effects of an intravenous serotonin challenge on breathing pattern were studied in 8 pentobarbitone-chloralose anaesthetised cats. Bol...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02256323
更新日期：2003-11-01 00:00:00
abstract：:The interactive role of rostral ventrolateral medulla (RVL) cardiovascular neurons and brain angiotensin II (Ang II) in regulating the arterial blood pressure was examined by recording simultaneously the spontaneous activity of these spinal projecting neurons and the arterial blood pressure in the pentobarbital-anesth...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02253100
更新日期：1996-05-01 00:00:00
abstract：BACKGROUND:Human keratinocytes and derived products are crucial for skin repair and regeneration. Despite substantial advances in engineered skin equivalents, their poor availability and immunorejection remain major challenges in skin grafting. METHODS:Induced keratinocyte-like cells (iKCs) were directly reprogrammed ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-020-00642-1
更新日期：2020-04-20 00:00:00
abstract：:The ARFP/F protein is synthesized from the +1 reading frame of the hepatitis C virus (HCV) core protein gene. The function of this protein remains unknown. To study the function of the HCV ARFP/F protein, we have conducted the yeast two-hybrid screening experiment to identify cellular proteins that may interact with t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9248-9
更新日期：2008-07-01 00:00:00
abstract：:Alterations in eIF3-p48/INT6 gene expression have been implicated in murine and human mammary carcinogenesis. We examined levels of INT6 protein in human tumors and determined that breast and colon tumors clustered into distinct groups based on levels of INT6 expression and clinicopathological variables. We performed ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-007-9149-3
更新日期：2007-05-01 00:00:00
abstract：:Accumulative evidence has supported the role of nitric oxide (NO) in a variety of normal physiological functions as well as many pathological conditions. In this study, we examined the possible diabetogenicity of NO by measuring the expression of the insulin receptor substrate (IRS)-1 in rat hepatocytes and skeletal m...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-006-9073-y
更新日期：2006-07-01 00:00:00
abstract：BACKGROUND:Stroke is one of the leading causes of neuronal death. Sesamin is known for neuroprotection by its antioxidant and anti-inflammatory properties but it lacks blood-brain barrier (BBB) activity. A panel of sesamin derivatives was screened and 3-bis (3-methoxybenzyl) butane-1,4-diol (BBD) was selected for high ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-21-15
更新日期：2014-02-18 00:00:00
abstract：BACKGROUND:The apolipoprotein E4 (apoE4) genotype is a major risk factor for developing late-onset Alzheimer's disease (AD). Inheritance of apoE4 is also associated with impairments in olfactory function in early stages of AD. In this project we examined the effects of the three common isoforms of human apoE (apoE2, ap...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-20-49
更新日期：2013-07-12 00:00:00
abstract：:Developmentally regulated endothelial cell locus-1 (Del1) is an embryonic angiogenic factor expressed in early embryonic endothelial cells, but recently has been found to be expressed in some forms of cancers including colon and breast cancers, and melanoma, and human cancer cell lines. Overexpression of Del1 accelera...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-33
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:The enzyme-prodrug system is considered a promising tool for tumor treatment when conjugated with a targeting molecule. The asparagine-glycine-arginine (NGR) motif is a developing and interesting targeting peptide that could specifically bind to aminopeptidase N (APN), which is an NGR receptor expressed on t...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-016-0227-6
更新日期：2016-01-22 00:00:00
abstract：BACKGROUND:Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certain inflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, our goal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties....
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-8
更新日期：2010-02-12 00:00:00
abstract：:Acetylcholine (ACh), the first neurotransmitter to be identified, regulate the activities of central and peripheral functions through interactions with muscarinic receptors. Changes in muscarinic acetylcholine receptor (mAChR) have been implicated in the pathophysiology of many major diseases of the central nervous sy...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-16-38
更新日期：2009-04-03 00:00:00
abstract：:The technology of "Lab-on-a-Chip" allows the synthesis and analysis of chemicals and biological substance within a portable or handheld device. The 3D printed structures enable precise control of various geometries. The combination of these two technologies in recent years makes a significant progress. The current app...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-017-0384-2
更新日期：2017-10-18 00:00:00
abstract：:The present study was to investigate the effects and action mechanisms of dehydroepiandrosterone (DHEA) on steroidogenesis in rat adrenal zona glomerulosa cells (ZG). ZG cells were incubated with DHEA in the presence or absence of angiotensin II (AngII), a high concentration of potassium, 8-Br-cAMP, forskolin, 25-OH-c...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-008-9241-3
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:ESBL-producing bacteria are a clinical problem in the management of diseases caused by these pathogens. Worldwide, systemic infections with BL enzymes are evolving by mutations from classical bla genes in an intensified manner and they continue to be transferred across species. RESULTS:E.cloacae BF1417 isol...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-015-0131-5
更新日期：2015-04-11 00:00:00
abstract：BACKGROUND:Chemotherapy and targeted therapies have made important strides in cancer treatment yet they often fail and new therapies are still needed. Here, we employed a phenotypic screen to identify and analyze the mechanism of action of novel small molecules that interfere with critical pathways involved in tumor ce...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-018-0408-6
更新日期：2018-02-08 00:00:00
abstract：BACKGROUND:Loss of ovarian function, as in menopause or after ovariectomy (OVX), is closely associated with obesity and white adipose tissue (WAT) inflammation. Estrogen replacement protects against postmenopausal obesity but increases the risks of carcinogenesis. In the present study, we investigated the effects of lo...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-019-0556-3
更新日期：2019-08-31 00:00:00
abstract：BACKGROUND:The type VI adenylyl cyclase (AC6) is a main contributor of cAMP production in the heart. The amino acid (aa) sequence of AC6 is highly homologous to that of another major cardiac adenylyl cyclase, AC5, except for its N-terminus (AC6-N, aa 1-86). Activation of AC6, rather than AC5, produces cardioprotective ...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/s12929-017-0367-3
更新日期：2017-09-04 00:00:00
abstract：:Retrograde transport of Wheat germ agglutinin conjugated to Horseradish peroxidase (WGA-HRP) was used in labeling vagal neurons projecting to the stomach from the dorsal motor nucleus of the vagus nerve (DMNV) in Streptozotocin (STZ)-induced diabetic rats. Diabetes was induced in the experimental rats by intraperitone...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/s11373-006-9097-3
更新日期：2006-09-01 00:00:00
abstract：:The sigma-1 receptor (Sig-1R) is a chaperone that resides mainly at the mitochondrion-associated endoplasmic reticulum (ER) membrane (called the MAMs) and acts as a dynamic pluripotent modulator in living systems. At the MAM, the Sig-1R is known to play a role in regulating the Ca2+ signaling between ER and mitochondr...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-017-0380-6
更新日期：2017-09-16 00:00:00
abstract：BACKGROUND:The association of TGF beta1 polymorphisms and atrial fibrillation (AF) in essential hypertensive (EH) subjects remains unknown. Methods EH subjects with AF (EH+AF+) and sinus rhythm (EH+AF-) were enrolled. The polymorphisms of +869 T --> C at codon 10 and + 915 G --> C at codon 25, were genotyped. The clini...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1186/1423-0127-17-23
更新日期：2010-03-31 00:00:00
abstract：:Reactive oxygen species (ROS) have been long considered simply as harmful by-products of metabolism, which damage cellular proteins, lipids, and nucleic acids. ROS are also known as a weapon of phagocytes, employed against pathogens invading the host. However, during the last decade, an understanding has emerged that ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/s12929-015-0194-3
更新日期：2015-10-15 00:00:00
abstract：:Experimental allergic encephalomyelitis (EAE) is an accepted animal model for the human demyelinating disease multiple sclerosis. The continuously propagated line of Lewis rat T helper lymphocytes (GP1 T cells), specific for the encephalitogenic 68-86 sequence of guinea pig myelin basic protein (GPMBP), mediates the a...
journal_title：Journal of biomedical science
pub_type： 杂志文章
doi：10.1007/BF02255600
更新日期：1997-03-01 00:00:00
abstract：:The nuclear envelope (NE) consists of two membrane layers that segregate the nuclear from the cytoplasmic contents. Recent progress in our understanding of nuclear-lamina associated diseases has revealed intriguing connections between the envelope components and nuclear processes. Here, we review the functions of the ...
journal_title：Journal of biomedical science
pub_type： 杂志文章,评审
doi：10.1186/1423-0127-16-96
更新日期：2009-10-22 00:00:00
