Abstract:
:Epidermal growth factor responsive (EGFr) neural progenitor (NP) cells have been shown to be a potential alternative tissue source for neural transplantation and for developmental study. We have shown that nonpassaged EGFr NP cells can self-renew for 2 years in neurospheres and can robustly differentiate into glia and a number of neuronal cell types. We are now attempting to investigate if the EGFr NP cells will die or continue to live beyond the life span of the donor. In addition, we and other investigators have also found that EGFr NP cells, after transplant, retain only a small number of cells in the transplant site. In this study, we investigate the plasticity and fate of the EGFr NP cells. Using the nonpassaged method, we found EGFr NP cells live in the EGF supplement medium for over 4 years-the longest-lived EGFr NP cells ever reported. The 4-year-old striatal or cortical EGFr neurospheres, when subplated with substrate coating, migrate out of neurospheres and have robust growth with many processes. Furthermore, when nucleotide marker bromodeoxyuridine (BrdU) was added 3 days prior to the subplating, the EGFr NP cells were labeled positively with BrdU in the nucleus, indicating active proliferation activity. Meanwhile two other events were also found in the long-term EGFr NP cells. In the midst of the proliferation, apoptosis occurred. A subpopulation of EGFr NP cells are undergoing programmed cell death as indicated by the cell morphology and the TUNEL staining for DNA strand breaks. The TUNEL fluorescein-staining indicates that over 50% of EGFr NP cells are positive in the nuclei. On the other hand, we have also found that the major base excision repair enzyme, APE/ref-1, which is responsible for recognizing and repairing baseless sites in DNA, was present in the progenitor cells. However, in those cells undergoing apoptosis, APE/ref-1 levels were dramatically reduced or missing, and only a small percentage of cells were TUNEL and APE/ref-1 positive. These observations indicate that EGFr neural progenitor cells can live beyond the life span of the donor animal. The longevity of these cells in culture may be enhanced due to decreased apoptosis and the retention of normal DNA repair capacity.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Zhou FC,Kelley MR,Chiang YH,Young Pdoi
10.1006/exnr.2000.7425keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
200-8issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(00)97425-9journal_volume
164pub_type
杂志文章abstract::The development of the spinal cord represents one of the most complex structure developments of the central nervous system (CNS) as it has to unfold along the longitudinal axis and within segmental cues. There it has to cope with on the one hand connection to the periphery (skeletal muscle, dermomyotome, smooth muscle...
journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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abstract::The beta-Amyloid peptide (A beta) is hypothesized to mediate the neurodegeneration seen in Alzheimer's disease. Recently, we proposed a new hypothesis to explain the toxicity of A beta based on the free-radical generating capacity of A beta. We have recently demonstrated using electron paramagnetic resonance (EPR) spe...
journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2007.04.004
更新日期:2007-07-01 00:00:00
abstract::Multiple sclerosis (MS), the most frequent inflammatory disease of the central nervous system (CNS), affects about two and a half million individuals worldwide and causes major burdens to the patients, which develop the disease usually at the age of 20 to 40. MS is likely referable to a breakdown of immune cell tolera...
journal_title:Experimental neurology
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pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.07.009
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2007.01.027
更新日期:2007-05-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7014
更新日期:1999-05-01 00:00:00
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journal_title:Experimental neurology
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pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.02.008
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90229-3
更新日期:1986-12-01 00:00:00
abstract::Clinical studies have provided evidence that the progression of HIV-1-associated neurocognitive disorders (HAND) involves alterations in dopamine (DA) systems. Drugs of abuse that act on the brain DA system, such as cocaine (Coc), may exacerbate HIV-1 infection and consequent behavioral and neurological manifestations...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2012.10.008
更新日期:2013-01-01 00:00:00
abstract::In what ways do passive touch and active touch require particular properties of cortical physiology? Because it is not easy to make predictions "bottom up" from neurophysiology and anatomy to behavior, there have been surprises in studying behavioral deficits following selective ablations of SI versus SII. For example...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1006/exnr.1994.1017
更新日期:1994-01-01 00:00:00
abstract::Pompe disease is an inherited disorder due to a mutation in the gene that encodes acid α-glucosidase (GAA). Children with infantile-onset Pompe disease develop progressive hypotonic weakness and cardiopulmonary insufficiency that may eventually require mechanical ventilation (MV). Our team conducted a first in human t...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2016.07.013
更新日期:2017-01-01 00:00:00
abstract::We report on a 41-year old woman with prolonged comatose unresponsiveness following traumatic head injury. Structural MRI showed bilateral midbrain damage and ventriculomegalia. Functional MRI revealed robust cortical responses to visual, auditory and tactile stimulation. Speech stimuli moreover consistently elicited ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.08.007
更新日期:2008-12-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1994.1070
更新日期:1994-04-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7194
更新日期:1999-11-01 00:00:00
abstract::The tibial functional index has been employed to assess functional recovery in rats following nerve repair by direct coaptation under tension or by grafting. Recovery after direct repair of small to moderate gap sizes was better than that after grafting. ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(91)90162-6
更新日期:1991-12-01 00:00:00