Herpes simplex virus vector-mediated expression of Bcl-2 protects spinal motor neurons from degeneration following root avulsion.

Abstract:

:Proximal axotomy in adult animals results in delayed death of motor neurons. Features characteristic of both necrosis and apoptosis have been described in motor neurons of the spinal cord following proximal avulsion of the ventral roots. We have previously demonstrated that a genomic herpes simplex virus (HSV)-based vector expressing the anti-apoptotic peptide Bcl-2 protects dopaminergic neurons of the substantia nigra from neurotoxin-induced apoptotic cell death and preserves the neurotransmitter phenotype of those cells. In this study we examined whether the same vector could protect adult rat lumbar motor neurons from cell death following proximal ventral root avulsion. Injection of the Bcl-2-expressing vector 1 week prior to root avulsion increased the survival of lesioned motor neurons, determined by retrograde Fluorogold labeling, by 50%. The Bcl-2-expressing vector did not preserve choline acetyltransferase neurotransmitter phenotype of the lesioned cells. These results shed light on the mechanism of cell death following axonal injury, and have implications for developing an effective treatment for the clinical problem of proximal root avulsion.

journal_name

Exp Neurol

journal_title

Experimental neurology

authors

Yamada M,Natsume A,Mata M,Oligino T,Goss J,Glorioso J,Fink DJ

doi

10.1006/exnr.2000.7597

keywords:

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

225-30

issue

2

eissn

0014-4886

issn

1090-2430

pii

S0014-4886(00)97597-6

journal_volume

168

pub_type

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