Abstract：
:The molecular composition and three-dimensional organization of the extracellular matrix (ECM) was studied by immunofluorescent microscopy, transmission and scanning electron microscopy in three connective tissue structures of the cochlea: the spiral limbus, basilar membrane and spiral ligament. Type II collagen, fibronectin, tenascin, chondroitin sulfate proteoglycans, alphav and beta1 integrins were immunolocalized in the ECM of these connective tissue structures. Electron micrographs showed a continuum of cross-striated collagen fibrils having a similar diameter and axial periodicity that spread from the spiral limbus via the basilar membrane and into the spiral ligament. Some of collagen fibrils were aggregated laterally into bundles. Bundle images, and their digital Fourier transformations, showed a major 67-nm axial D-repeat characteristic for collagen fibrils. Transmission electron microscopy showed numerous proteoglycans associated with the collagen fibrils. The spiral limbus, basilar membrane and spiral ligament demonstrated regional differences in molecular composition and structural organization of their ECM. The glycoproteins fibronectin, tenascin and alphav integrin were immunolocalized mainly in the basilar membrane. Collagen fibrils of the spiral limbus and spiral ligament did not appear to be strongly oriented. However, most of the collagen fibrils in the basilar membrane were arranged into radially directed bundles. Collagen fibrils in the basilar membrane were also surrounded by a homogeneous matrix, which was immunoreactive to fibronectin and tenascin antibodies. A more complete understanding of the composition and structural organization of the ECM in these connective tissue structures in the cochlea provides a foundation upon which micromechanical models of cochlear function can be constructed.
journal_name
Hear Resjournal_title
Hearing researchauthors
Tsuprun V,Santi Pdoi
10.1016/s0378-5955(98)00219-6keywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
35-49issue
1-2eissn
0378-5955issn
1878-5891pii
S0378-5955(98)00219-6journal_volume
129pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract：:The standard procedure to study temporal encoding of sound waveforms in the auditory system has been Fourier analysis of responses to periodic stimuli. We introduce a new metric--correlation index (CI)--which is based on a simple counting of spike coincidences. It can be used for responses to aperiodic stimuli and doe...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2006.03.010
更新日期：2006-06-01 00:00:00
abstract：:Prior research [Caine et al., 1981] suggested that scopolamine, a central cholinergic antagonist, may increase gap thresholds in young human listeners. If confirmed, an effect of scopolamine on gap detection might help to explain why both aged humans and aged laboratory animals have less sensitive temporal acuity on g...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(00)00088-5
更新日期：2000-07-01 00:00:00
abstract：:Speech is central to human life. As such, any delay or impairment in receptive speech processing can have a profoundly negative impact on the social and professional life of a person. Thus, being able to assess the integrity of speech processing in different populations is an important goal. Current standardized asses...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2017.02.015
更新日期：2017-05-01 00:00:00
abstract：:The endocochlear potential (EP) provides part of the electrochemical drive for sound-driven currents through cochlear hair cells. Intense noise exposure (110 dB SPL, 2 h) differentially affects the EP in three inbred mouse strains (C57BL/6 [B6], CBA/J [CBA], BALB/cJ [BALB]) (Ohlemiller and Gagnon, 2007, Hearing Resear...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2018.01.015
更新日期：2018-04-01 00:00:00
abstract：:The auditory filter may be considered as a weighting function representing frequency selectivity at a particular centre frequency. Its shape can be derived using the power-spectrum model of masking which assumes: (1) in detecting a signal in a masker the observer uses the single auditory filter giving the highest sign...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(87)90050-5
更新日期：1987-01-01 00:00:00
abstract：OBJECTIVE:This paper introduces a new method to calculate relative risks of elevated hearing thresholds, at various ages and frequencies, between a study population and ISO1999:2003: Annex A Screened, Annex B Unscreened and ISO1999 Section 5.3 adjustment for noise exposure using Annex A Screened data. We demonstrate th...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2012.01.007
更新日期：2012-03-01 00:00:00
abstract：:This study was aimed at defining the relationship between noise-related hearing impairment in industrial workers exposed to continuous noise. For this malondialdehyde and glutathione peroxidase were analyzed as free radical form and antioxidant form. A total of 60 patients working in the units of a hydroelectric power...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(01)00365-3
更新日期：2001-12-01 00:00:00
abstract：:The olivocochlear bundle was sectioned at the floor of the fourth ventricle in a series of cats. From three to thirty weeks post-operatively, recordings were made from single auditory-nerve fibers. Tuning curves, spontaneous discharge rates, and rate-level functions for tones at the characteristic frequency were measu...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(90)90105-x
更新日期：1990-11-01 00:00:00
abstract：:A critical issue in large-scale gene expression analysis is the impact of sexually dimorphic genes, which may confound the results when sampling across sexes. Here, we assessed, for the first time, sex differences at the transcriptome level in the auditory brainstem. To this end, microarray experiments covering the wh...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2008.08.008
更新日期：2008-11-01 00:00:00
abstract：:It is widely appreciated that the key predictor of the pitch of a sound is its periodicity. Neural structures which support pitch perception must therefore be able to reflect the repetition rate of a sound, but this alone is not sufficient. Since pitch is a psychoacoustic property, a putative cortical code for pitch m...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2010.04.015
更新日期：2011-01-01 00:00:00
abstract：:Hearing aid (HA) users can differ markedly in their benefit from directional processing (or beamforming) algorithms. The current study therefore investigated candidacy for different bilateral directional processing schemes. Groups of elderly listeners with symmetric (N = 20) or asymmetric (N = 19) hearing thresholds f...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2017.07.014
更新日期：2017-09-01 00:00:00
abstract：:In natural environments, temporally complex signals often occur in a background of noise. The neural mechanisms underlying the preservation of temporal sensitivity in background noise are poorly understood. In the present study, we examined the ability of inferior colliculus (IC) units with primary-like and sustained ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(00)00186-6
更新日期：2000-12-01 00:00:00
abstract：:It has previously been demonstrated that ototoxicity induced by systemic administration of cisplatin is reduced by concomitant systemic administration of alpha-melanocyte stimulating hormone (alpha-MSH). In this study we investigated the effects of cochlear, perilymphatic application of alpha-MSH during intraperitonea...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/S0378-5955(03)00396-4
更新日期：2004-03-01 00:00:00
abstract：:Wavelength-swept optical coherence tomography (OCT) was used to scan the structure of cadaveric chinchilla ears in three dimensions with high spatial resolution and measure the sound-induced displacements of the entire OCT-visible lateral surfaces of the ossicles in the lateral-to-medial direction. The simultaneous me...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2020.108056
更新日期：2020-10-01 00:00:00
abstract：:The effects of genotype and diet on age-related hearing loss were evaluated using auditory brainstem response (ABR) thresholds and post-mortem cochlear histopathology in 5 inbred mouse strains, CBA/H-T6J (CH), DBA/2J (D2), C57BL/6J (B6), BALB/cByJ (BY) and WB/ReJ (WB), and their 10 F1 hybrid strains. The mice had been...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(95)00107-f
更新日期：1995-08-01 00:00:00
abstract：:Activation of heat shock factors (Hsfs) is one of the potential mechanisms for regulating the transcription of the heat shock proteins (Hsps) and certain other stress-responsive genes. Reverse transcription polymerase chain reaction (RT-PCR), Western blot and immunocytochemistry were used to examine the expression and...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(02)00607-x
更新日期：2002-11-01 00:00:00
abstract：:The analysis of the auditory scene begins from the moment we hear sounds, making it possible for the infant to distinguish the mother's voice from other sounds in the environment. The purpose of the study was to determine, in two experiments, whether the frequency separation threshold, at which the perception of a mix...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2006.12.013
更新日期：2007-03-01 00:00:00
abstract：:Cyclic histograms of the responses of single auditory ganglion cells in the guinea pig were recorded during stimulation with amplitude modulated tones. Modulation frequencies ranged from 10 Hz to 800 Hz. The response histograms, phase-locked to the modulation signals, were analysed for mean action potential rate and f...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(87)90003-7
更新日期：1987-01-01 00:00:00
abstract：:We are studying the interconnections between the anteroventral cochlear nucleus (AVCN) and the dorsal cochlear nucleus (DCN). Biotinylated dextran was injected into the DCN, where the best frequency of responses was also recorded. Ventrotubercular neurons in AVCN were labeled, along with cochlear nerve fibers and the ...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(98)00224-x
更新日期：1999-04-01 00:00:00
abstract：:The incidence and severity of audiogenic seizures in kanamycin (KM)-treated rat pups, from a Wistar strain which is inherently seizure-resistant, was analyzed as a function of (a) postnatal age at the time of KM injection (i.p.) and (b) KM dosage. The vigor of the pinna reflex response on postnatal day (PND) 28 was co...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(88)90142-6
更新日期：1988-01-01 00:00:00
abstract：:Older adults, even with clinically normal hearing sensitivity, have auditory perceptual deficits relative to their younger counterparts. This difficulty may in part, be related to a decline in the neural representation of frequency. The purpose of this study was to examine the effect of age on behavioral and physiolog...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/j.heares.2009.11.010
更新日期：2010-06-01 00:00:00
abstract：:Both sound exposure and gentamicin treatment cause damage to sensory hair cells in the peripheral chick auditory organ, the basilar papilla. This induces a regeneration response which replaces hair cells and restores auditory function. Since functional recovery requires the re-establishment of connections between rege...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(97)00150-0
更新日期：1997-11-01 00:00:00
abstract：:The inner ear contains receptor cells that oscillate spontaneously, generating waves that propagate backward in the cochlea, ultimately causing sound to be radiated into the ear canal--the spontaneous otoacoustic emissions (SOAEs). Except in rare conditions, these internally generated signals appear to go unheard. The...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(02)00388-x
更新日期：2002-09-01 00:00:00
abstract：:The directional dependence of sound pressure transformation of head and pinna has been measured in the phyllostomid bat Phyllostomus discolor for the frontal hemisphere using a maximum length sequence method. The azimuthal position of the axis of highest pinna gain came closer to the midsagital plane with increasing f...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(03)00164-3
更新日期：2003-07-01 00:00:00
abstract：:In the developing inner ear, a naturally occurring programmed cell death of cochleovestibular ganglion (CVG) neurons as well as peripheral and central target-derived trophic effects on survival of embryonic CVG neurons are known. To further analyze these target derived trophic interactions, spiral ganglion explants ob...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(92)90127-9
更新日期：1992-03-01 00:00:00
abstract：:Blind cave fish use the lateral line sensory system to detect nearby objects. The fish responds to sudden perturbations in the water column by initiating startle responses in which they swim more rapidly. Normal startle responses disappear after trauma caused by a single 15 s immersion in calcium free water, but retur...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(01)00226-x
更新日期：2001-04-01 00:00:00
abstract：:Glass microelectrodes were introduced into the stria vascularis (SV) to measure the DC potential profile of the SV in vivo from its apical as well as basal side. The K+ concentration gradient in the lateral cochlear wall was measured by means of double barrel K+-selective microelectrodes. A positive potential 10.4 +/-...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(87)90077-3
更新日期：1987-01-01 00:00:00
abstract：:The glycoconjugates in the cochlea of the guinea pig were studied by staining samples by the silver methenamine method as well as after periodic acid-Schiff (PAS) staining. Results obtained by the two methods were similar but not identical. The silver methenamine method was much better in terms of resolution. However,...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/0378-5955(93)90234-r
更新日期：1993-05-01 00:00:00
abstract：:The highly variable benefit of hearing devices is a serious challenge in auditory rehabilitation. Various factors contribute to this phenomenon such as the diversity in ear defects, the different extent of auditory nerve hypoplasia, the age of intervention, and cognitive abilities. Recent analyses indicate that, in ad...
journal_title：Hearing research
pub_type： 杂志文章,评审
doi：10.1016/j.heares.2014.02.004
更新日期：2014-06-01 00:00:00
abstract：:Nitric oxide (NO) not only has normal physiological roles like vasodilation and neurotransmission in the living organism, it could also have possible neurodestructive effects under certain pathological conditions. The present study aimed to determine whether direct exposure of guinea pig cochlea to a NO donor like sod...
journal_title：Hearing research
pub_type： 杂志文章
doi：10.1016/s0378-5955(97)00159-7
更新日期：1997-12-01 00:00:00
