Abstract:
:Nitric oxide (NO) not only has normal physiological roles like vasodilation and neurotransmission in the living organism, it could also have possible neurodestructive effects under certain pathological conditions. The present study aimed to determine whether direct exposure of guinea pig cochlea to a NO donor like sodium nitroprusside (SNP), or a nitric oxide synthase (NOS) inhibitor like N(G)-nitro-L-arginine methyl ester (L-NAME), would cause damage to the auditory hair cells. A piece of gelfoam was placed on the round window of the right ear of adult albino guinea pigs. It was then soaked with 0.1 ml of SNP (3.4 microM), 0.1 ml of L-NAME (9.3 microM or 18.5 microM) or 0.1 ml of injection water, the vehicle used to dissolve the above chemicals. Twelve animals receiving SNP were perfused 1 day, 2, 3 and 7 days later, with three animals being used for each survival period. Six animals receiving L-NAME were allowed to survive up to 7 days before perfusion. Eight animals receiving injection water or 0.45% saline were used as controls. With the scanning electron microscope, the inner and outer hair cells were counted over a 1 mm length of the basilar membrane in each turn of every cochlea. The results showed that, in animals treated with L-NAME at both concentrations stated, no significant loss of either inner or outer hair cells was noted in any part of the cochlea studied. However, as early as 1 day after SNP treatment, a striking loss of inner and outer hair cells was observed in the three lower turns of the cochlea. Damage to the outer hair cells was extended to the apical turn with increasing survival period, but no significant loss of inner hair cells was evident in the apical turn at any of the survival periods studied. To rule out the possibility that the effects were due to the presence of cyanide, a metabolite of SNP, hydroxycobalamin was introduced into the scala tympani of three animals through a cannula-osmotic pump device during SNP treatment. There was no significant difference in the results between the groups with and without hydroxycobalamin infusion 7 days after SNP treatment. The present study suggests that an excessive production of NO in the inner ear could lead to extensive loss of hair cells.
journal_name
Hear Resjournal_title
Hearing researchauthors
Ruan RS,Leong SK,Yeoh KHdoi
10.1016/s0378-5955(97)00159-7subject
Has Abstractpub_date
1997-12-01 00:00:00pages
169-78issue
1-2eissn
0378-5955issn
1878-5891pii
S0378-5955(97)00159-7journal_volume
114pub_type
杂志文章相关文献
HEARING RESEARCH文献大全abstract::The otoprotective peptide AM-111, a cell-permeable inhibitor of JNK mediated apoptosis, was tested for its efficacy as a rescue agent following impulse noise trauma. Single dose administrations of AM-111 at 1h or 4h post-impulse noise exposure (155 dB peak SPL) via systemic or local routes were evaluated with a total ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2006.05.006
更新日期:2007-04-01 00:00:00
abstract::In this paper, research on the temporal and spatial distribution of cholinergic-related molecules in the lower auditory brainstem, with an emphasis on nicotinic acetylcholine receptors (nAChRs), is reviewed. The possible functions of acetylcholine (ACh) in driving selective auditory neurons before the onset of hearing...
journal_title:Hearing research
pub_type: 杂志文章,评审
doi:10.1016/j.heares.2005.02.012
更新日期:2005-08-01 00:00:00
abstract::Selected mechanical features of the design of the homodyne interferometer are described. This interferometer was utilized for basilar membrane vibration measurements. The massive construction necessary to minimize mechanical vibration and noise is described. In order to measure basilar membrane vibrations, it was nece...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(86)90173-5
更新日期:1986-01-01 00:00:00
abstract::Cochlear implant (CI) users typically have excellent speech recognition in quiet but struggle with understanding speech in noise. It is thought that broad current spread from stimulating electrodes causes adjacent electrodes to activate overlapping populations of neurons which results in interactions across adjacent c...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.02.004
更新日期:2013-05-01 00:00:00
abstract::A number of studies have shown that the ear can be protected from sound over-exposure, either by activating the cochlear efferent system, or by sound 'conditioning' in which the role of the efferent system is less certain. To study more definitively the molecular basis of deliberately induced cochlear protection from ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(01)00265-9
更新日期:2001-06-01 00:00:00
abstract::Gaumond et al. [(1982) J. Neurophysiol. 48, 856-873] showed in the cat that a multiplicative-intensity model can generally account quite well for reduction of the probability of an auditory-nerve spike by another spike preceding it by 4 to 25 ms, and that for smaller separations there is also an increased latency of t...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(93)90093-g
更新日期:1993-09-01 00:00:00
abstract::Sodium salicylate (SS) can penetrate the blood-brain barrier to target neurons in the central auditory system. Understanding how SS alters functional behaviors of different types of central auditory neurons will provide insights into the neural mechanisms of SS-induced tinnitus. Here, we report the differential effect...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2009.03.007
更新日期:2009-07-01 00:00:00
abstract::Adenosine plays a major cytoprotective role during ischemia and conditions of oxidative stress. Previous studies in our laboratory indicate that oxidative stress induces expression of the A1 adenosine receptor (A1AR) via activation of nuclear factor (NF)-kappaB. In this study, we tested whether noise exposure could in...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/S0378-5955(03)00344-7
更新日期:2004-02-01 00:00:00
abstract::Cochlear implant performance in difficult listening situations is limited by channel interactions. It is known that partial tripolar (PTP) stimulation reduces the spread of excitation (SOE). However, the greater the degree of current focusing, the greater the absolute current required to maintain a fixed loudness. As ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2011.12.009
更新日期:2012-02-01 00:00:00
abstract::Cortical auditory evoked potentials (CAEPs) have been successfully used to explore the effects of noise on speech processing at the cortical level in adults and children. The purpose of this study was to determine whether +15 dB signal-to-noise ratios (SNRs), often recommended for optimal speech perception in children...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2020.108165
更新日期:2021-01-05 00:00:00
abstract::Georg von Békésy was one of the first comparative auditory researchers. He not only studied basilar membrane (BM) movements in a range of mammals of widely different sizes, he also worked on the chicken basilar papilla and the frog middle ear. We show that, in mammals, at least, his data do not differ from those that ...
journal_title:Hearing research
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.heares.2012.04.013
更新日期:2012-11-01 00:00:00
abstract::This study is the continuation of the functional probing of the auditory periphery in the normal and jaundiced rat. Threshold tuning curves from normal rat auditory nerve fibers were comparable to those reported in other mammals. Life-long unconjugated hyperbilirubinemia does not appear to have a widespread, demonstra...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(91)90139-z
更新日期:1991-07-01 00:00:00
abstract::The effect of interaural time differences (ITDs) on stream segregation for successive tone bursts was investigated. Obligatory stream segregation was inferred from the threshold for detecting a rhythmic irregularity in an otherwise isochronous sequence of interleaved "A" and "B" tones (task 1). Subjective stream segre...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2012.06.006
更新日期:2012-09-01 00:00:00
abstract::The barn owl (Tyto alba) uses interaural level difference (ILD) as a cue for the localization of sound. The first site of binaural convergence in the pathway that processes ILD is the ventral lateral lemniscus pars posterior (VLVp). Neurons in VLVp receive excitatory input from the contralateral nucleus angularis, and...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(98)00102-6
更新日期:1998-09-01 00:00:00
abstract::A novel immunocytochemical method was used to determine whether the sound-damaged adult quail ear can repair its tectorial membrane (TM) and to compare the repair in quail to that in chicks. Birds were exposed to an octave band noise with a center frequency of 1.5 kHz at 116 dB SPL for 4 h. The chicks were grouped bas...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(95)00051-5
更新日期:1995-06-01 00:00:00
abstract::The auditory system is thought to process complex sounds through overlapping bandpass filters. Frequency selectivity as estimated by auditory filters has been well quantified in humans and other mammalian species using behavioral and physiological methodologies, but little work has been done to examine frequency selec...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2017.11.012
更新日期:2018-01-01 00:00:00
abstract::The primary goal of most cochlear implant procedures is to improve a patient's ability to discriminate speech. To accomplish this, cochlear implants are programmed so as to maximize speech understanding. However, programming a cochlear implant can be an iterative, labor-intensive process that takes place over months. ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2013.11.007
更新日期:2014-03-01 00:00:00
abstract::Acetylcholine (ACh) is the dominant transmitter released from inner ear efferent neurons. In frog vestibular organs, these efferent neurons synapse exclusively with type II hair cells. Hair cells isolated from the frog saccule hyperpolarize following the application of 50 microM ACh, thereby demonstrating the presence...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(00)00092-7
更新日期:2000-08-01 00:00:00
abstract::It has been demonstrated that INS can be utilized to stimulate spiral ganglion cells in the cochlea. Although neural stimulation can be achieved without direct contact of the radiation source and the tissue, the presence of fluids or bone between the target structure and the radiation source may lead to absorption or ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2011.06.006
更新日期:2011-12-01 00:00:00
abstract::We determined velocity discrimination thresholds and Weber fractions for sounds revolving around the listener at very high velocities. Sounds used were a broadband white noise and two harmonic sounds with fundamental frequencies of 330 Hz and 1760 Hz. Experiment 1 used velocities ranging between 288°/s and 720°/s in a...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2014.07.014
更新日期:2014-10-01 00:00:00
abstract::The cochlear microphonic (CM) is produced by a change in standing currents during the motion of the cochlear partition. The motion of the partition and associated hair cell transduction processes are nonlinear and are reflected in the variation of the instantaneous frequency (IF) of the CM. Although the CM is dominate...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(02)00642-1
更新日期:2002-12-01 00:00:00
abstract::Laser Doppler vibrometry was used to measure the sound-induced tympanic membrane (TM) velocity, assessed near the umbo, in 56 normal hearing human subjects at nine sound frequencies. A second series of measurements was made in 47 subjects with sensorineural hearing loss (SNHL). Each set of measurements has features in...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(03)00332-0
更新日期:2004-01-01 00:00:00
abstract::We are studying the interconnections between the anteroventral cochlear nucleus (AVCN) and the dorsal cochlear nucleus (DCN). Biotinylated dextran was injected into the DCN, where the best frequency of responses was also recorded. Ventrotubercular neurons in AVCN were labeled, along with cochlear nerve fibers and the ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(98)00224-x
更新日期:1999-04-01 00:00:00
abstract::Localization of calmodulin, a calcium binding protein, was identified in adult gerbil cochleas using paraffin section immunohistochemistry and immunogold electron microscopy with monoclonal antibody against bovine calmodulin. Immunoreactive calmodulin was abundant in inner hair cells (IHCs), outer hair cells (OHCs) an...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(00)00220-3
更新日期:2001-01-01 00:00:00
abstract::Measurements were made of inner hair cell receptor potentials and basilar membrane motion in the 17-21 kHz region of the guinea pig cochlea. The latter were made using the Mossbauer technique. Isoamplitude curves at 0.9 mV d.c. receptor potential were compared with isovelocity curves at 0.04 mm/s and the corresponding...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(83)90019-9
更新日期:1983-04-01 00:00:00
abstract::The discharge properties of 735 single units located in the pars magnocellularis (M) of the medial division of the medial geniculate body (MGB) were studied in 23 nitrous oxide anesthetized cats in response to simple acoustic stimuli (clicks, noise and tone bursts). A systematic decrease of single unit characteristic ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/0378-5955(89)90086-5
更新日期:1989-05-01 00:00:00
abstract::Several electrophysiological and psychophysical studies have shown that the spatial excitation pattern produced by bipolar stimulation of a cochlear implant (CI) can have a dual-peak shape. The perceptual effects of this dual-peak shape were investigated using noise-vocoded CI simulations in which synthesis filters we...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2014.11.001
更新日期:2015-01-01 00:00:00
abstract::Carboplatin is currently being used as an anticancer drug against human cancers. However, high dose of carboplatin chemotherapy resulted in hearing loss in cancer patients. We have shown that carboplatin-induced hearing loss was related to dose-dependent oxidative injury to the cochlea in rat model. However, the time ...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2004.01.011
更新日期:2004-05-01 00:00:00
abstract:OBJECTIVE:This paper introduces a new method to calculate relative risks of elevated hearing thresholds, at various ages and frequencies, between a study population and ISO1999:2003: Annex A Screened, Annex B Unscreened and ISO1999 Section 5.3 adjustment for noise exposure using Annex A Screened data. We demonstrate th...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/j.heares.2012.01.007
更新日期:2012-03-01 00:00:00
abstract::Light microscopy and immunohistochemical analyses of a freshly prepared human cochlea, removed at meningioma skull base surgery, were performed with particular emphasis on synaptophysin (SY) reactivity. Synaptophysin, a 38-kDa glycoprotein, is one of the most abundant integral membrane proteins of small presynaptic ve...
journal_title:Hearing research
pub_type: 杂志文章
doi:10.1016/s0378-5955(03)00228-4
更新日期:2003-11-01 00:00:00