Hypoxia-reoxygenation and polyunsaturated fatty acids modulate adrenergic functions in cultured cardiomyocytes.

abstract：:The key information processing units within gene regulatory networks are enhancers. Enhancer activity is associated with the production of tissue-specific noncoding RNAs, yet the existence of such transcripts during cardiac development has not been established. Using an integrated genomic approach, we demonstrate that...

journal_title：Journal of molecular and cellular cardiology

authors： Ounzain S,Pezzuto I,Micheletti R,Burdet F,Sheta R,Nemir M,Gonzales C,Sarre A,Alexanian M,Blow MJ,May D,Johnson R,Dauvillier J,Pennacchio LA,Pedrazzini T

更新日期：2014-11-01 00:00:00

abstract：:Ventricular trabeculae from adult and newborn ferret heart were chemically skinned by Triton X-100 which disrupts all cellular membranes. In newborn preparations, maximal Ca(2+)-activated tension was two times smaller than in adult fibres while Ca2+ sensitivity was higher. Caffeine (10 mM) and cyclopiazonic acid (100 ...

journal_title：Journal of molecular and cellular cardiology

authors： Bonnet V,Léoty C

更新日期：1996-06-01 00:00:00

abstract：:CD34+ progenitor cells hold promise for therapeutic neovascularization in various settings. In this study, the role of human peripheral blood CD34+ cells in neovascularization and inflammatory cell recruitment was longitudinally studied in vivo. Human CD34+ cells were incorporated in Matrigel, implanted subcutaneously...

journal_title：Journal of molecular and cellular cardiology

authors： van der Strate BW,Popa ER,Schipper M,Brouwer LA,Hendriks M,Harmsen MC,van Luyn MJ

更新日期：2007-06-01 00:00:00

abstract：:The effects of preconditioning, adenosine and dipyridamole in protecting the systolic and diastolic alterations of myocardial stunning in rabbit hearts were studied. Isovolumic left ventricular developed pressure (LVDP), and end diastolic pressure (LVEDP) were measured. The time constant of relaxation (T) was calculat...

journal_title：Journal of molecular and cellular cardiology

authors： Mosca SM,Gelpi RJ,Cingolani HE

更新日期：1994-10-01 00:00:00

abstract：:Insulin increases the synthesis of mitochondrial proteins in the isolated perfused heart and total cell protein synthesis in neonatal cardiac myocytes. Since carnitine-dependent fatty acid oxidation is modulated by insulin in a variety of tissues, the effects of 1.7 microM insulin on the mitochondrial enzyme(s), carni...

journal_title：Journal of molecular and cellular cardiology

authors： Hudson EK,Liu MH,Buja LM,McMillin JB

更新日期：1995-01-01 00:00:00

abstract：:Atrial cardiac myocytes secrete the vasoactive hormone atrial natriuretic peptide (ANP) by both constitutive and regulated exocytotic fusion of ANP-containing large dense core vesicles (LDCV) with the sarcolemma. Detailed information, however, regarding the identity and function of specific membrane fusion proteins (S...

journal_title：Journal of molecular and cellular cardiology

authors： Peters CG,Miller DF,Giovannucci DR

更新日期：2006-03-01 00:00:00

abstract：:We have previously proposed that the heterogeneous collapse of mitochondrial inner membrane potential (DeltaPsi(m)) during ischemia and reperfusion contributes to arrhythmogenesis through the formation of metabolic sinks in the myocardium, wherein clusters of myocytes with uncoupled mitochondria and high K(ATP) curren...

journal_title：Journal of molecular and cellular cardiology

authors： Brown DA,Aon MA,Frasier CR,Sloan RC,Maloney AH,Anderson EJ,O'Rourke B

更新日期：2010-04-01 00:00:00

abstract：:Effects of hypoxia-reoxygenation (H-R) on myocytes isolated from 10 week hypertrophied and sham control rat hearts were studied. Myocyte hypertrophy was indicated by an increase in cell size. Superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) enzyme activities were significantly higher and lipid peroxidatio...

journal_title：Journal of molecular and cellular cardiology

authors： Kirshenbaum LA,Hill M,Singal PK

更新日期：1995-01-01 00:00:00

abstract：:In the postnatal rat heart, muscle cells continue to divide as well as increase in size. At the same time the cells in the soleus muscle (a slow skeletal muscle) do not divide, although they continue to grow in size. Since microtubules may have a role in orienting intracellular structures in muscle, we determined the ...

journal_title：Journal of molecular and cellular cardiology

authors： Cartwright J Jr,Goldstein MA

更新日期：1985-01-01 00:00:00

abstract：:The authors have previously resolved four forms of cyclic nucleotide phosphodiesterase (PDE) in neonatal rat cultured cardiomyocytes by use of high-performance liquid chromatography (HPLC) and have shown that the response of the cGMP-stimulated PDE to the effector cGMP was markedly reduced as compared to that of the c...

journal_title：Journal of molecular and cellular cardiology

authors： Picq M,Dubois M,Grynberg A,Lagarde M,Prigent AF

更新日期：1996-10-01 00:00:00

abstract：:The specialized cardiac conduction system (CCS) consists of the sinoatrial node (SAN) and the atrioventricular (AV) conduction system (AVCS), which includes proximal (AV node, bundle of His and bundle branches) and distal (Purkinje fibers) components. In four CCS marker mice [two transgenic (cGATA6|lacZ, CCS|lacZ) and...

journal_title：Journal of molecular and cellular cardiology

authors： Viswanathan S,Burch JB,Fishman GI,Moskowitz IP,Benson DW

更新日期：2007-05-01 00:00:00

abstract：:Studies using chemically-induced models of diabetes have shown the diabetic myocardium to exhibit abnormalities in cellular ion transport, which may affect susceptibility to reperfusion-induced arrhythmias. We studied the incidence of reperfusion-induced ventricular tachycardia (VT) and fibrillation (VF) in isolated h...

journal_title：Journal of molecular and cellular cardiology

authors： Kusama Y,Hearse DJ,Avkiran M

更新日期：1992-04-01 00:00:00

abstract：:Microtubules have been implicated in a number of muscle-specific functions, including sarcomerogenesis and the regulation of heart cell beating rate. Post-translationally modified microtubules (MTs) have been correlated, in other cell types, with MTs of increased stability and possibly distinct functions. This study w...

journal_title：Journal of molecular and cellular cardiology

authors： Webster DR

更新日期：1997-06-01 00:00:00

abstract：:To investigate the interaction of cytoskeleton with the receptor modulation of ionic currents, we studied the effect of muscarinic and beta-adrenergic stimulation in adult guinea-pig ventricular cardiac myocytes treated with paclitaxel and colchicine, two drugs that respectively stabilize or destabilize microtubules. ...

journal_title：Journal of molecular and cellular cardiology

authors： Malan D,Gallo MP,Bedendi I,Biasin C,Levi RC,Alloatti G

更新日期：2003-02-01 00:00:00

abstract：:To test the hypothesis that alterations in adrenergic or cholinergic receptors occur in response to physical training, and that changes in receptor properties could be mechanistically important in producting the altered cardiovascular physiology of the trained state, we studied the effects of endurance training by swi...

journal_title：Journal of molecular and cellular cardiology

authors： Williams RS,Schaible TF,Bishop T,Morey M

更新日期：1984-05-01 00:00:00

abstract：:The effects of the inhibition of phospholipid degradation and superoxide radical generation on prostaglandin synthesis associated with myocardial ischemia and reperfusion were studied in the isolated, in-situ pig heart model subjected to 60 mins of regional ischemia and a further 60 mins of hypothermic potassium cardi...

journal_title：Journal of molecular and cellular cardiology

authors： Otani H,Engelman RM,Rousou JA,Breyer RH,Das DK

更新日期：1986-09-01 00:00:00

abstract：:We examined metabolic effects of "supply" and "demand" ischemia in immature and mature rabbit hearts. Moderate supply ischemia was produced by a 50% reduction in coronary flow (to approximately 5.0 ml min-1 g-1 giving a 50-55% rise in O2 extraction and a 35% drop in O2 supply/demand). Demand ischemia was produced by s...

journal_title：Journal of molecular and cellular cardiology

authors： Matherne GP,Ely SW,Headrick JP

更新日期：1996-05-01 00:00:00

abstract：:Cardiac arrhythmias are one of the main causes of death worldwide. Several studies have shown that inflammation plays a key role in different cardiac diseases and Toll-like receptors (TLRs) seem to be involved in cardiac complications. In the present study, we investigated whether the activation of TLR4 induces cardia...

journal_title：Journal of molecular and cellular cardiology

authors： Monnerat-Cahli G,Alonso H,Gallego M,Alarcón ML,Bassani RA,Casis O,Medei E

更新日期：2014-11-01 00:00:00

abstract：:Myocardial perfusion measurement with colored microspheres may become an alternative for radioactive microsphere techniques. We use and validate a spectrophotometric method that has been previously established for large animals in the isolated perfused rat heart. The perfusion system was adapted for use in a NMR micro...

journal_title：Journal of molecular and cellular cardiology

authors： Hiller KH,Adami P,Voll S,Roder F,Kowallik P,Bauer WR,Haase A,Ertl G

更新日期：1996-03-01 00:00:00

abstract：:We made novel measurements of the influence of rest intervals and stimulation frequency on twitch contractions and on sarcoplasmic reticulum (SR) Ca(2+)-content (using rapid cooling contractures, RCCs) in isolated ventricular muscle strips from rat and rabbit hearts at a physiological temperature of 37 degrees C. In a...

journal_title：Journal of molecular and cellular cardiology

authors： Maier LS,Bers DM,Pieske B

更新日期：2000-12-01 00:00:00

abstract：:Heart failure of diverse causes is associated with abnormalities of sarcoplasmic reticulum (SR) Ca(2+)transport. The purpose of this study was to determine whether the thyroid hormone analogue, 3,5-diiodothyropropionic acid (DITPA), prevents abnormal Ca(2+)transport and expression of SR proteins associated with post-i...

journal_title：Journal of molecular and cellular cardiology

authors： Pennock GD,Spooner PH,Summers CE,Litwin SE

更新日期：2000-11-01 00:00:00

abstract：:The ability of the beta-receptor antagonist propranolol to influence the response of isolated cardiac and vascular smooth muscle to several classes of calcium channel blockers was examined. For comparison, the interactions between propranolol and other classes of negative inotropic and vasorelaxant agents was also eva...

journal_title：Journal of molecular and cellular cardiology

authors： Weishaar RE,Quade MM,Pugsley TA,Shih YH,Taylor DG Jr

更新日期：1988-10-01 00:00:00

abstract：INTRODUCTION:Fibroblast activation protein α (FAP) is a membrane-bound serine protease expressed by activated fibroblasts during wound healing in the skin. Expression of FAP after myocardial infarction (MI) and potential effects on cardiac wound healing are largely unknown. METHODS:MI was induced in rats and FAP expre...

journal_title：Journal of molecular and cellular cardiology

authors： Tillmanns J,Hoffmann D,Habbaba Y,Schmitto JD,Sedding D,Fraccarollo D,Galuppo P,Bauersachs J

更新日期：2015-10-01 00:00:00

abstract：:Recent data indicates that DJ-1 plays a role in the cellular response to stress. Here, we aimed to examine the underlying molecular mechanisms mediating the actions of DJ-1 in the heart following myocardial ischemia-reperfusion (I/R) injury. In response to I/R injury, DJ-1 KO mice displayed increased areas of infarcti...

journal_title：Journal of molecular and cellular cardiology

authors： Shimizu Y,Lambert JP,Nicholson CK,Kim JJ,Wolfson DW,Cho HC,Husain A,Naqvi N,Chin LS,Li L,Calvert JW

更新日期：2016-08-01 00:00:00

abstract：:Cell-based therapy after myocardial infarction (MI) is a promising therapeutic option but the relevant cell subsets and dosage requirements are poorly defined. We hypothesized that cell therapy for myocardial infarction is improved by ex vivo expansion and high-dose transplantation of defined hematopoietic progenitor ...

journal_title：Journal of molecular and cellular cardiology

authors： Templin C,Kotlarz D,Faulhaber J,Schnabel S,Grote K,Salguero G,Luchtefeld M,Hiller KH,Jakob P,Naim HY,Schieffer B,Hilfiker-Kleiner D,Landmesser U,Limbourg FP,Drexler H

更新日期：2008-09-01 00:00:00

abstract：:Reoxygenation of isolated rat cardiac myocytes following a period of hypoxia and substrate deprivation resulted in a 1.5-2-fold increase in the total Ca2+ content which could be inhibited by 1 microM antimycin A or ruthenium red (50% inhibition at 2.5 microM). This increase in Ca2+ content was not accompanied by any r...

journal_title：Journal of molecular and cellular cardiology

authors： Stone D,Darley-Usmar V,Smith DR,O'Leary V

更新日期：1989-10-01 00:00:00

abstract：:Understanding the process of Ca(2+)/Mg(2+)exchange during muscle excitation and relaxation is fundamental to elucidating the mechanism of Ca(2+)-regulated muscle contraction. During the resting phase, the C-domain of cardiac troponin C may be occupied by either Ca(2+)or Mg(2+). Here, complexes of recombinant cardiac t...

journal_title：Journal of molecular and cellular cardiology

authors： Finley N,Dvoretsky A,Rosevear PR

更新日期：2000-08-01 00:00:00

abstract：:Previous studies have demonstrated that acute ethanol exposure induces activation of delta protein kinase C (deltaPKC) and epsilonPKC, and mimics ischemic preconditioning via epsilonPKC activation. However, the role of deltaPKC isozyme in ischemia and reperfusion is still controversial. Here, we investigated the role ...

journal_title：Journal of molecular and cellular cardiology

authors： Inagaki K,Mochly-Rosen D

更新日期：2005-08-01 00:00:00

abstract：:Vascular cells are very sensitive to their hemodynamic environment. Any change in blood pressure or blood flow can be sensed by endothelial and vascular smooth muscle cells and ultimately results in structural modifications within the vascular wall that accommodate the new conditions. In the case of hypertension, the ...

journal_title：Journal of molecular and cellular cardiology

authors： Lemarié CA,Tharaux PL,Lehoux S

更新日期：2010-03-01 00:00:00

abstract：:Our previous studies showed that homocysteine (Hcy) reduces endothelial progenitor cell (EPC) numbers and impairs functional activity. However, the mechanisms by which Hcy reduces EPCs numbers and activity remain to be determined. Recent studies have demonstrated that reduced EPCs numbers and activity was associated w...

journal_title：Journal of molecular and cellular cardiology

authors： Zhu JH,Chen JZ,Wang XX,Xie XD,Sun J,Zhang FR

更新日期：2006-05-01 00:00:00