Differences in Ca(2+)-handling and sarcoplasmic reticulum Ca(2+)-content in isolated rat and rabbit myocardium.

abstract：:We recently showed that angiotensin (ANG) II as well as mechanical stretch stimulated production of tumor necrosis factor (TNF) in cardiac fibroblasts. Presently, we examined the molecular mechanisms by which ANGII and lipopolysaccharide (LPS) upregulate TNF-alpha gene expression. In neonatal rat cardiac fibroblasts, ...

journal_title：Journal of molecular and cellular cardiology

authors： Sato H,Watanabe A,Tanaka T,Koitabashi N,Arai M,Kurabayashi M,Yokoyama T

更新日期：2003-10-01 00:00:00

abstract：:Atrial fibrillation (AF) is commonly associated with chronic dilatation of the left atrium, both in human disease and animal models. The immediate signaling enzyme phospholipase C (PLC) is activated by mechanical stretch to generate the Ca2+-releasing messenger inositol(1,4,5)trisphosphate (Ins(1,4,5)P3) and sn-1,2-di...

journal_title：Journal of molecular and cellular cardiology

authors： Woodcock EA,Grubb DR,Filtz TM,Marasco S,Luo J,McLeod-Dryden TJ,Kaye DM,Sadoshima J,Du XJ,Wong C,McMullen JR,Dart AM

更新日期：2009-11-01 00:00:00

abstract：:Rapamycin (Sirolimus®) is used to prevent rejection of transplanted organs and coronary restenosis. We reported that rapamycin induced cardioprotection against ischemia-reperfusion (I/R) injury through opening of mitochondrial K(ATP) channels. However, signaling mechanisms in rapamycin-induced cardioprotection are cur...

journal_title：Journal of molecular and cellular cardiology

authors： Das A,Salloum FN,Durrant D,Ockaili R,Kukreja RC

更新日期：2012-12-01 00:00:00

abstract：:Endothelin-1 (ET-1) is a potent agonist of cell growth that also stimulates Na(+)/H(+) exchanger isoform 1 (NHE-1) activity. It was hypothesized that the increase in intracellular Na(+) ([Na(+)](i)) mediated by NHE-1 activity may induce the reverse mode of Na(+)/Ca(2+) exchanger (NCX(rev)) increasing intracellular Ca(...

journal_title：Journal of molecular and cellular cardiology

authors： Dulce RA,Hurtado C,Ennis IL,Garciarena CD,Alvarez MC,Caldiz C,Pierce GN,Portiansky EL,Chiappe de Cingolani GE,Camilión de Hurtado MC

更新日期：2006-11-01 00:00:00

abstract：:Mitochondria are central players in the pathophysiology of ischemia-reperfusion. Activation of plasma membrane G-coupled receptors or the Na,K-ATPase triggers cytosolic signaling pathways that result in cardioprotection. Our working hypothesis is that the occupied receptors migrate to caveolae, where signaling enzymes...

journal_title：Journal of molecular and cellular cardiology

authors： Garlid KD,Costa AD,Quinlan CL,Pierre SV,Dos Santos P

更新日期：2009-06-01 00:00:00

abstract：:Atrial tachyarrhythmias, the most common type of cardiac arrhythmias, are associated with greater stroke risk. Muscarinic cholinergic agonists have been shown to facilitate atrial tachyarrhythmia maintenance in the absence of cardiac disease. This has been attributed to action potential shortening, which enhances myoc...

journal_title：Journal of molecular and cellular cardiology

authors： Zafalon N Jr,Oshiyama NF,Bassani JW,Bassani RA

更新日期：2013-12-01 00:00:00

abstract：:The 23Na NMR shift-reagent complexes (Dy(PPP)2, Dy(TTHA), and Tm(DOTP)) bind stoichiometric amounts of Ca2+. Thus, in perfused rat heart systems, a supplementation of Ca2+ is required to maintain the requisite extracellular free calcium concentration ([Ca(o)]f) and to approximate a physiological level of contractile f...

journal_title：Journal of molecular and cellular cardiology

authors： Gaszner B,Simor T,Hild G,Elgavish GA

更新日期：2001-11-01 00:00:00

abstract：:Cardiac myocytes in rat hearts lose their ability to undergo cytokinesis between day 3 and day 4, resulting in the formation of binucleated myocytes. Failure in the formation of the actin-myosin contractile ring could cause cardiac myocytes to be defective in cytokinesis. Enzymatically isolated cardiac myocytes from 2...

journal_title：Journal of molecular and cellular cardiology

authors： Li F,Wang X,Bunger PC,Gerdes AM

更新日期：1997-06-01 00:00:00

abstract：:The effect of linoleic and arachidonic acid derivatives on ATP-dependent calcium transport was studied in the isolated vesicles from cardiac sarcoplasmic reticulum of guinea-pigs. Oxidation products of linoleic and arachidonic acids, obtained either by autoxidation or incubation with soybean lipoxygenase, effectively ...

journal_title：Journal of molecular and cellular cardiology

authors： Kim RS,LaBella FS

更新日期：1988-02-01 00:00:00

abstract：:The transformation of vascular smooth muscle cells [VSMC] into foam cells leading to increased plaque size and decreased stability is a key, yet understudied step in atherogenesis. We reported that Interleukin-19 (IL-19), a novel, anti-inflammatory cytokine, attenuates atherosclerosis by anti-inflammatory effects on V...

journal_title：Journal of molecular and cellular cardiology

authors： Gabunia K,Herman AB,Ray M,Kelemen SE,England RN,DeLa Cadena R,Foster WJ,Elliott KJ,Eguchi S,Autieri MV

更新日期：2017-04-01 00:00:00

abstract：:Changes in the capacities of ATP-synthesizing reactions were analysed in residual non-infarcted myocardium following myocardial infarction. Rats were subjected to left coronary artery ligation (MI; n = 11) or to sham operation (sham; n = 18). Two months later, hearts were excised, rinsed and buffer-perfused isovolumic...

journal_title：Journal of molecular and cellular cardiology

authors： Laser A,Ingwall JS,Tian R,Reis I,Hu K,Gaudron P,Ertl G,Neubauer S

更新日期：1996-07-01 00:00:00

abstract：:This study describes the effect of oxygen radicals on the ultrastructure of the isolated Langendorff-perfused rat heart. Oxygen radicals were enzymatically generated by xanthine oxidase (0.025 U/ml) and hypoxanthine (0.96 mM). Hearts were perfusion-fixed for electron microscopy and stereological technique was performe...

journal_title：Journal of molecular and cellular cardiology

authors： Ytrehus K,Myklebust R,Olsen R,Mjøs OD

更新日期：1987-04-01 00:00:00

abstract：:Atrial cardiac myocytes secrete the vasoactive hormone atrial natriuretic peptide (ANP) by both constitutive and regulated exocytotic fusion of ANP-containing large dense core vesicles (LDCV) with the sarcolemma. Detailed information, however, regarding the identity and function of specific membrane fusion proteins (S...

journal_title：Journal of molecular and cellular cardiology

authors： Peters CG,Miller DF,Giovannucci DR

更新日期：2006-03-01 00:00:00

abstract：:In single guinea pig ventricular cells, genistein, a potent inhibitor of protein tyrosine kinase (PTK), was found to suppress the delayed-rectifier K (IK) current. The present study was carried out to examine the underlying mechanism. Ventricular myocytes were voltage-clamped in the conventional whole-cell mode (36 de...

journal_title：Journal of molecular and cellular cardiology

authors： Washizuka T,Horie M,Obayashi K,Sasayama S

更新日期：1998-12-01 00:00:00

abstract：:The two-pore domain potassium (K(+)) channel TWIK-1 (or K2P1.1) contributes to background K(+) conductance in diverse cell types. TWIK-1, encoded by the KCNK1 gene, is present in the human heart with robust expression in the atria, however its physiological significance is unknown. To evaluate the cardiac effects of T...

journal_title：Journal of molecular and cellular cardiology

authors： Christensen AH,Chatelain FC,Huttner IG,Olesen MS,Soka M,Feliciangeli S,Horvat C,Santiago CF,Vandenberg JI,Schmitt N,Olesen SP,Lesage F,Fatkin D

更新日期：2016-08-01 00:00:00

abstract：:Free radicals and lipid peroxides have recently been identified by us [1, 2, 3] as metabolic intermediates during acute myocardial ischemia. The mechanisms by which evolving myocardial ischemia initiates free radical production are not clear. Based on studies in vitro, it is feasible to consider the following possibil...

journal_title：Journal of molecular and cellular cardiology

authors： Rao PS,Cohen MV,Mueller HS

更新日期：1983-10-01 00:00:00

abstract：:The heart responds to increased haemodynamic load with growth of the ventricles. The rise in ventricle mass is due to increasing mass of the myocytes and proliferation of fibroblasts and smooth muscle cells. The accompanying adaptation and remodelling of the interstitium, e.g. production and composition of the extrace...

journal_title：Journal of molecular and cellular cardiology

authors： Simm A,Nestler M,Hoppe V

更新日期：1997-01-01 00:00:00

abstract：:We have tested the hypothesis that thyroid state may influence both the flow of cellular Ca2+ and the myofilament response to Ca2+ by effects on intracellular pH (pHi) and Na+ (Nai+). Single cardiac myocytes isolated from hypothyroid, euthyroid and hyperthyroid animals were loaded with fura-2/AM (Cai2+ probe), BCECF/A...

journal_title：Journal of molecular and cellular cardiology

authors： Wolska BM,Averyhart-Fullard V,Omachi A,Stojanović MO,Kallen RG,Solaro RJ

更新日期：1997-10-01 00:00:00

abstract：:The purposes of this study were to: (1) assess myocardial pyruvate dehydrogenase (PDH) activity and substrate exchange under well-perfused and ischemic conditions; (2) determine the metabolic effects of an intra-coronary infusion of the PDH activator, dichloroacetate (DCA); and (3) measure the effects of ischemia and ...

journal_title：Journal of molecular and cellular cardiology

authors： Stanley WC,Hernandez LA,Spires D,Bringas J,Wallace S,McCormack JG

更新日期：1996-05-01 00:00:00

abstract：:The opening of mitochondrial permeability transition pore (PTP) during reperfusion injury of heart has been well demonstrated and thus controlling PTP would attenuate the myocardial damage and cell death. Ursodeoxycholic acid (UDCA) is a hydrophilic bile salt and has been shown to prevent apoptosis in hepatocytes by i...

journal_title：Journal of molecular and cellular cardiology

authors： Rajesh KG,Suzuki R,Maeda H,Yamamoto M,Yutong X,Sasaguri S

更新日期：2005-11-01 00:00:00

abstract：:The resting potential of "sodium-loaded' cardiac cells can transiently hyperpolarize to levels negative to the steady state resting potential [3,5,6]. Hyperpolarization is associated with the coupled efflux of Na+ and influx of K+ driven by an active transport process and may result from an increased K+ equilibrium po...

journal_title：Journal of molecular and cellular cardiology

authors： Rasmussen HH,TenEick RE,McCullough JR,Singer DH

更新日期：1984-08-01 00:00:00

abstract：:This study addressed the question whether the molecular mass of proteins influences their release from isolated rat neonatal cardiomyocytes subjected to simulated ischemia (SI) or metabolic inhibition (MI). During these interventions cellular ATP content and the relative releases of several proteins, ranging in molecu...

journal_title：Journal of molecular and cellular cardiology

authors： Van Nieuwenhoven FA,Musters RJ,Post JA,Verkleij AJ,Van der Vusse GJ,Glatz JF

更新日期：1996-07-01 00:00:00

abstract：:Isolated and perfused rat hearts were examined by 2-dimensional gel electrophoresis and liquid scintillation counting to determine the effect of ischemia or perfusion temperature on protein synthesis. Isolated hearts were subjected to ischemia at either 4 degrees, 20 degrees, or 30 degrees C and then perfused at 37 de...

journal_title：Journal of molecular and cellular cardiology

authors： Currie RW

更新日期：1987-08-01 00:00:00

abstract：:Cardiac myosin binding protein C (cMyBP-C) phosphorylation is differentially regulated in the normal heart and during disease development. Our objective was to examine in detail three phosphorylatable sites (Ser-273, Ser-282, and Ser-302) present in the protein's cardiac-specific sequences, as these residues are diffe...

journal_title：Journal of molecular and cellular cardiology

authors： Gupta MK,Gulick J,James J,Osinska H,Lorenz JN,Robbins J

更新日期：2013-11-01 00:00:00

abstract：:Lack of endothelial nitric oxide synthase (eNOS) may affect the sensitivity of cyclic GMP signaling through soluble guanylyl cyclase (sGC). We hypothesized that in eNOS knockout (eNOS-/-) mice, stimulation of guanylyl cyclase would have enhanced effects inhibiting cardiac contraction. We measured cell shortening and c...

journal_title：Journal of molecular and cellular cardiology

authors： Su J,Tse J,Scholz PM,Weiss HR

更新日期：2005-12-01 00:00:00

abstract：:Glypicans are a group of membrane-bound heparan sulfate proteoglycans (HSPG) that are tissue specific and developmentally regulated. Transcripts for avian glypican are found in endocardial cushions, limb buds, somites and forebrain of early chick embryos. Since avian glypican is not well characterized, the cellular lo...

journal_title：Journal of molecular and cellular cardiology

authors： Niu S,Bahl JJ,Adamson C,Morkin E

更新日期：1998-03-01 00:00:00

abstract：:Lysophosphatidylcholine (LPC) accumulation in intracellular and/or interstitial space in cardiomyocytes may underlie as a mechanism for tachycardia and various arrhythmias during cardiac ischemia, which is usually accompanied by elevation of intracellular Ca(2+) concentration ([Ca(2+)](i)). The present study was there...

journal_title：Journal of molecular and cellular cardiology

authors： Zheng M,Wang Y,Kang L,Shimaoka T,Marni F,Ono K

更新日期：2010-01-01 00:00:00

abstract：:Pacemaker (HCN) channels have a key role in the generation and modulation of spontaneous activity of sinoatrial node myocytes. Previous work has shown that compartmentation of HCN4 pacemaker channels within caveolae regulates important functions, but the molecular mechanism responsible is still unknown. HCN channels h...

journal_title：Journal of molecular and cellular cardiology

authors： Barbuti A,Scavone A,Mazzocchi N,Terragni B,Baruscotti M,Difrancesco D

更新日期：2012-08-01 00:00:00

abstract：:Congenital heart block (CHB) affects offspring of mothers with autoantibodies (positive IgG) to intracellular SSA/Ro and SSB/La ribonucleoproteins and is associated with high morbidity and mortality. Here, we show that maternal anti-Ro/La antibodies immunoreact with human fetal cardiomyocyte sarcolemma, recognize huma...

journal_title：Journal of molecular and cellular cardiology

authors： Qu Y,Xiao GQ,Chen L,Boutjdir M

更新日期：2001-06-01 00:00:00

abstract：:Nkx2.5 and serum response factor (SRF) are critically important transcription factors in cardiac morphogenesis. They are also widely expressed in adult cardiomyocytes, but there is little data to indicate their possible role in adult cardiac cells. In this paper we demonstrate that the interaction of Nkx2.5 and SRF in...

journal_title：Journal of molecular and cellular cardiology

authors： Müller JG,Thompson JT,Edmonson AM,Rackley MS,Kasahara H,Izumo S,McQuinn TC,Menick DR,O'Brien TX

更新日期：2002-07-01 00:00:00