当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > A caveolin-binding domain in the HCN4 channels mediates functional interaction with caveolin proteins.

A caveolin-binding domain in the HCN4 channels mediates functional interaction with caveolin proteins.

Abstract：

:Pacemaker (HCN) channels have a key role in the generation and modulation of spontaneous activity of sinoatrial node myocytes. Previous work has shown that compartmentation of HCN4 pacemaker channels within caveolae regulates important functions, but the molecular mechanism responsible is still unknown. HCN channels have a conserved caveolin-binding domain (CBD) composed of three aromatic amino acids at the N-terminus; we sought to evaluate the role of this CBD in channel-protein interaction by mutational analysis. We generated two HCN4 mutants with a disrupted CBD (Y259S, F262V) and two with conservative mutations (Y259F, F262Y). In CHO cells expressing endogenous caveolin-1 (cav-1), alteration of the CBD shifted channels activation to more positive potentials, slowed deactivation and made Y259S and F262V mutants insensitive to cholesterol depletion-induced caveolar disorganization. CBD alteration also caused a significant decrease of current density, due to a weaker HCN4-cav-1 interaction and accumulation of cytoplasmic channels. These effects were absent in mutants with a preserved CBD. In caveolin-1-free fibroblasts, HCN4 trafficking was impaired and current density reduced with all constructs; the activation curve of F262V was not altered relative to wt, and that of Y259S displayed only half the shift than in CHO cells. The conserved CBD present in all HCN isoforms mediates their functional interaction with caveolins. The elucidation of the molecular details of HCN4-cav-1 interaction can provide novel information to understand the basis of cardiac phenotypes associated with some forms of caveolinopathies.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Barbuti A,Scavone A,Mazzocchi N,Terragni B,Baruscotti M,Difrancesco D

doi

10.1016/j.yjmcc.2012.05.013

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

187-95

issue

2

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(12)00190-3

journal_volume

53

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Flecainide inhibits arrhythmogenic Ca2+ waves by open state block of ryanodine receptor Ca2+ release channels and reduction of Ca2+ spark mass.

    abstract：:Catecholaminergic polymorphic ventricular tachycardia (CPVT) is linked to mutations in the cardiac ryanodine receptor (RyR2) or calsequestrin. We recently found that the drug flecainide inhibits RyR2 channels and prevents CPVT in mice and humans. Here we compared the effects of flecainide and tetracaine, a known RyR2 ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.10.005

    authors： Hilliard FA,Steele DS,Laver D,Yang Z,Le Marchand SJ,Chopra N,Piston DW,Huke S,Knollmann BC

    更新日期：2010-02-01 00:00:00

  • Characterization of thromboxane A2/prostaglandin H2 binding sites in guinea pig cardiac membrane preparations.

    abstract：:Thromboxane A2 (TXA2) and prostaglandin H2 (PGH2) induce platelet aggregation and are potent vasoconstrictors, and they have been implicated in coronary vasospasm and myocardial infarction. The TXA2 mimetic [1S-(1 alpha, 2 beta (5Z), 3 alpha (1E,3S*), 4 alpha)]-7-[3-(3-hydroxy-4-(4'- iodophenoxy)-1-butenyl)-7-oxabicyc...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1109

    authors： Bowling N,Dubé GP,Kurtz WL,Brune KA,Saussy DL Jr,Dorn GW 2nd,Mais DE

    更新日期：1994-07-01 00:00:00

  • Regulation of post-translationally modified microtubule populations during neonatal cardiac development.

    abstract：:Microtubules have been implicated in a number of muscle-specific functions, including sarcomerogenesis and the regulation of heart cell beating rate. Post-translationally modified microtubules (MTs) have been correlated, in other cell types, with MTs of increased stability and possibly distinct functions. This study w...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1997.0421

    authors： Webster DR

    更新日期：1997-06-01 00:00:00

  • PDGF-BB enhances monocyte chemoattractant protein-1 mRNA stability in smooth muscle cells by downregulating ribonuclease activity.

    abstract：:Platelet-derived growth factor (PDGF) has protean manifestations, including the regulation of growth and migration, in many cell types. We have previously reported that PDGF-BB induces the accumulation of monocyte chemoattractant protein (MCP)-1 mRNA in smooth muscle cells (SMC), in large part due to an increase in mR...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.03.426

    authors： Liu B,Poon M,Taubman MB

    更新日期：2006-07-01 00:00:00

  • Enhanced effect of myocardial gene transfection by VP22-mediated intercellular protein transport.

    abstract：:One of the major issues in myocardial gene therapy is poor transfection efficiency. The herpes simplex virus protein VP22 is known to facilitate intercellular protein transport. Not only VP22 but also VP22-linked protein are exported from the cytoplasm of cells, in which it is synthesised endogenously, and transferred...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.01.007

    authors： Suzuki K,Murtuza B,Brand NJ,Varela-Carver A,Fukushima S,Yacoub MH

    更新日期：2004-04-01 00:00:00

  • Intracellular angiotensin II fusion protein alters AT1 receptor fusion protein distribution and activates CREB.

    abstract：:In recently published studies, we show that angiotensin II (AII) generated from an engineered rat angiotensinogen cDNA, and maintained intracellularly, is growth stimulatory for a rat hepatoma cell line. In the present study, we report that co-expression of AII fused to cyan fluorescent protein (ECFP/AII) and angioten...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2003.09.021

    authors： Cook JL,Re R,Alam J,Hart M,Zhang Z

    更新日期：2004-01-01 00:00:00

  • Duality of innate stress responses in cardiac injury, repair, and remodeling.

    abstract：:The ability of the myocardium to successfully adapt to cardiac injury ultimately determines whether the heart will decompensate and fail, or whether instead it will maintain preserved function. Despite the importance of the myocardial response to cardiac injury, very little is known with respect to the biochemical mec...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2004.05.028

    authors： Wilson EM,Diwan A,Spinale FG,Mann DL

    更新日期：2004-10-01 00:00:00

  • Impaired p38 MAPK/HSP27 signaling underlies aging-related failure in opioid-mediated cardioprotection.

    abstract：:Cardioprotection and preconditioning mediated via G-protein-coupled receptors may be lost or impaired with advancing age, limiting ischemic tolerance and the ability to pharmacologically protect older hearts from ischemic injury. Our preliminary findings indicated a loss of delta-opioid receptor-mediated protection in...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.02.011

    authors： Peart JN,Gross ER,Headrick JP,Gross GJ

    更新日期：2007-05-01 00:00:00

  • Characterization of contractile function in diabetic hypertensive cardiomyopathy in adult rat ventricular myocytes.

    abstract：:Diabetes and hypertension both produce myocardial dysfunction that accelerates cardiovascular morbidity and mortality. Coexistence of the two often results in a more severe cardiomyopathy than either process alone. The purpose of this study was to characterize the contractile function of diabetic hypertensive cardiomy...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1431

    authors： Wold LE,Relling DP,Colligan PB,Scott GI,Hintz KK,Ren BH,Epstein PN,Ren J

    更新日期：2001-09-01 00:00:00

  • Ischemia-induced noradrenaline release in the isolated rat heart: influence of perfusion substrate and duration of ischemia.

    abstract：:To examine some of the characteristics of the local noradrenaline (NA) release in myocardial ischemia a study was made on Langendorff-perfused rat hearts, prelabelled with 3H-NA. The left coronary artery was ligated for 15, 30, 60 or 120 min, followed by 10 min of reperfusion. The coronary effluent was collected and a...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(83)90344-9

    authors： Abrahamsson T,Almgren O,Carlsson L

    更新日期：1983-12-01 00:00:00

  • Lysozyme binding to endocardial endothelium mediates myocardial depression by the nitric oxide guanosine 3',5' monophosphate pathway in sepsis.

    abstract：:Inflammatory mediators have been implicated as a cause of reversible myocardial depression in septic shock. We previously reported that the release of lysozyme-c (Lmz-S) from leukocytes from the spleen or other organs contributes to myocardial dysfunction in Escherichia coli septic shock in dogs by binding to a cardia...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.06.009

    authors： Mink SN,Bose R,Roberts DE,Jacobs H,Duke K,Bose D,Cheng ZQ,Light RB

    更新日期：2005-10-01 00:00:00

  • Structural vs. contractile protein remodeling and myocardial stiffness in hypertrophied rat left ventricle.

    abstract：:Left ventricular pressure overload will result in the hypertrophic growth of the myocardium and in the rat may include a remodeling of both the structural and contractile proteins. As a result, an adaptive rise in active stiffness, or the force generating capacity of the myocardium, may occur. The relative importance ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(88)90597-4

    authors： Jalil JE,Doering CW,Janicki JS,Pick R,Clark WA,Abrahams C,Weber KT

    更新日期：1988-12-01 00:00:00

  • Metabolism, hypoxia and the diabetic heart.

    abstract：:The diabetic heart becomes metabolically remodelled as a consequence of exposure to abnormal circulating substrates and hormones. Fatty acid uptake and metabolism are increased in the type 2 diabetic heart, resulting in accumulation of intracellular lipid intermediates and an increased contribution of fatty acids towa...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2011.01.007

    authors： Heather LC,Clarke K

    更新日期：2011-04-01 00:00:00

  • Stable high level expression of a transfected human HSP70 gene protects a heart-derived muscle cell line against thermal stress.

    abstract：:Heat shock protein 70 (HSP70) has been shown to play a fundamental role in the induction of thermotolerance in many biological systems. Elevated synthesis of HSP70 in response to diverse stresses such as heat, anoxia, ischaemia, ethanol and heavy metals has been correlated with protection against subsequent more sever...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1084

    authors： Heads RJ,Latchman DS,Yellon DM

    更新日期：1994-06-01 00:00:00

  • Stimulation of the AT2 receptor reduced atherogenesis in ApoE(-/-)/AT1A(-/-) double knock out mice.

    abstract：:AT1 receptor blockers (ARB) and in part ACE inhibitors (ACI) potentially exert beneficial effects on atherogenesis independent of AT1 receptor inhibition. These pleiotropic effects might be related to angiotensin II mediated activation of the AT2 receptor. To analyze this hypothesis we investigated the development of ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2011.12.007

    authors： Tiyerili V,Mueller CF,Becher UM,Czech T,van Eickels M,Daiber A,Nickenig G,Wassmann S

    更新日期：2012-03-01 00:00:00

  • Arrhythmogenic coupling between the Na+ -Ca2+ exchanger and inositol 1,4,5-triphosphate receptor in rat pulmonary vein cardiomyocytes.

    abstract：:Atrial fibrillation, the most common sustained arrhythmia, is believed to be triggered by ectopic electrical activity originating in the myocardial sleeves surrounding the pulmonary veins (PVs). It has been reported that myocardial sleeves have the potential to generate automaticity in response to norepinephrine. This...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.01.007

    authors： Okamoto Y,Takano M,Ohba T,Ono K

    更新日期：2012-05-01 00:00:00

  • Intra-tracheal gene delivery of aerosolized SERCA2a to the lung suppresses ventricular arrhythmias in a model of pulmonary arterial hypertension.

    abstract：BACKGROUND:Pulmonary arterial hypertension (PAH) results in right ventricular (RV) failure, electro-mechanical dysfunction and heightened risk of sudden cardiac death (SCD), although exact mechanisms and predisposing factors remain unclear. Because impaired chronotropic response to exercise is a strong predictor of ear...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.11.017

    authors： Strauss B,Sassi Y,Bueno-Beti C,Ilkan Z,Raad N,Cacheux M,Bisserier M,Turnbull IC,Kohlbrenner E,Hajjar RJ,Hadri L,Akar FG

    更新日期：2019-02-01 00:00:00

  • Intracellular pH during hypoxia in normal and hypertrophied right ventricle of ferret heart.

    abstract：:The effects of preventing oxidative phosphorylation on pHi were compared in papillary muscles from right ventricles of normal and pressure-overloaded ferret hearts. Hypertrophy was induced by pulmonary artery clipping for 30-45 days. pHi was recorded with pH-sensitive microelectrodes. Resting pHi and the relationship ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(95)90043-8

    authors： Do E,Baudet S,Gow IF,Ellis D,Noireaud J

    更新日期：1995-03-01 00:00:00

  • Adenosine and dipyridamole mimic the effects of ischemic preconditioning.

    abstract：:The effects of preconditioning, adenosine and dipyridamole in protecting the systolic and diastolic alterations of myocardial stunning in rabbit hearts were studied. Isovolumic left ventricular developed pressure (LVDP), and end diastolic pressure (LVEDP) were measured. The time constant of relaxation (T) was calculat...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1158

    authors： Mosca SM,Gelpi RJ,Cingolani HE

    更新日期：1994-10-01 00:00:00

  • Effects of neuropeptide Y on collateral development in a swine model of chronic myocardial ischemia.

    abstract：:We investigated the role of neuropeptide Y (NPY), abundant in the myocardial sympathetic nervous system and endothelial cells, in angiogenesis during chronic myocardial ischemia. Adult male Yorkshire swine underwent ameroid constrictor placement on the proximal left circumflex coronary artery. After 3 weeks, an osmoti...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2010.08.022

    authors： Robich MP,Matyal R,Chu LM,Feng J,Xu SH,Laham RJ,Hess PE,Bianchi C,Sellke FW

    更新日期：2010-12-01 00:00:00

  • Oxygen diffusion distance in thyroxine-induced hypertrophic rabbit myocardium.

    abstract：:We studied the oxygen diffusion distance in the rabbit myocardium in untreated animals and in animals treated with thyroxine (T4) for 3 days and 16 days. The subepicardial and subendocardial regions were studied separately. Sixteen days of T4 treatment results in significant cardiac hypertrophy. After 16 days, the per...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(88)80146-9

    authors： Seiden D,Navidad P,Weiss HR

    更新日期：1988-10-01 00:00:00

  • The external work-pressure time integral relationships and the afterload dependence of Frank-Starling mechanism.

    abstract：:The mechanisms underlying the Frank-Starling Law of the heart are elusive and the prevalent notion suggests that it is afterload independent. However, isolated fiber studies reveal that the afterload determines cardiac function through cross-bridge dependent mechanisms. The study explores the roles of the afterload, i...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.05.007

    authors： Sela G,Landesberg A

    更新日期：2009-10-01 00:00:00

  • Zinc improves mitochondrial respiratory function and prevents mitochondrial ROS generation at reperfusion by phosphorylating STAT3 at Ser727.

    abstract：:Serine 727 (Ser727) phosphorylation of STAT3 plays a role in the regulation of mitochondrial respiration. This study aimed to test if zinc could regulate mitochondrial respiration through phosphorylation of STAT3 at Ser727 in the setting of ischemia/reperfusion in the heart. Under normoxic conditions, treatment of iso...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.03.019

    authors： Zhang G,Sheng M,Wang J,Teng T,Sun Y,Yang Q,Xu Z

    更新日期：2018-05-01 00:00:00

  • Sarcoplasmic reticulum Ca2+ pump blockade decreases O2 use of unloaded contracting rat heart slices: thapsigargin and cyclopiazonic acid.

    abstract：:We previously established a new measuring method of the myocardial O2 consumption of mechanically unloaded rat left-ventricular slices. O 2 consumption of unstimulated myocardium corresponds to basal metabolism. We have found O2 consumption of stimulated myocardium to include basal metabolism and O 2 consumption for C...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1997.0630

    authors： Takaki M,Kohzuki H,Kawatani Y,Yoshida A,Ishidate H,Suga H

    更新日期：1998-03-01 00:00:00

  • Mechanisms of action of beta-adrenergic agents on the arrhythmogenic transient inward current in rabbit Purkinje fibers.

    abstract：:Catecholamines increase the amplitudes of oscillatory afterpotentials (OAP) and peak magnitude of the transient inward current (Iti) responsible for OAP. The objectives of this study were to determine whether beta-adrenoceptor stimulation can induce Iti, and to determine the mechanism by which beta-adrenoceptor stimul...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(91)90047-p

    authors： Han XQ,Ferrier GR

    更新日期：1991-05-01 00:00:00

  • Bones of contention: marrow-derived cells in myocardial regeneration.

    abstract：:Almost 7 years have passed since the initial publication reporting that bone marrow cells regenerate infarcted myocardium. The subsequent years produced hundreds of investigations that ran the gamut of findings from validation to disproof. Undeterred by the concurrent debate, clinical trials ensued to test the safety ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2008.03.007

    authors： Sussman MA,Murry CE

    更新日期：2008-06-01 00:00:00

  • Protein kinase C activation enhances the delayed rectifier potassium current in guinea-pig heart cells.

    abstract：:The possible involvement of protein kinase C in modulating membrane currents was investigated in isolated guinea-pig ventricular cells. In a Na(+)-and K(+)-free external solution, the delayed rectifier K+ current (IK) was increased by the activator of protein kinase C (PKC), 12-O-tetradecanoylphorbol-13-acetate (TPA)....

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(90)91015-y

    authors： Tohse N,Kameyama M,Sekiguchi K,Shearman MS,Kanno M

    更新日期：1990-06-01 00:00:00

  • Microtubules mobility affects the modulation of L-type I(Ca) by muscarinic and beta-adrenergic agonists in guinea-pig cardiac myocytes.

    abstract：:To investigate the interaction of cytoskeleton with the receptor modulation of ionic currents, we studied the effect of muscarinic and beta-adrenergic stimulation in adult guinea-pig ventricular cardiac myocytes treated with paclitaxel and colchicine, two drugs that respectively stabilize or destabilize microtubules. ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(02)00312-7

    authors： Malan D,Gallo MP,Bedendi I,Biasin C,Levi RC,Alloatti G

    更新日期：2003-02-01 00:00:00

  • Interleukin-1beta attenuates beta-very low-density lipoprotein uptake and its receptor expression in vascular smooth muscle cells.

    abstract：:The very low-density lipoprotein (VLDL) receptor is a member of the low-density lipoprotein (LDL) receptor gene family with distinct tissue distribution and function. VLDL receptors are also expressed in vascular smooth muscle cells (VSMCs) and have been shown to be upregulated in atherosclerotic lesions. In the prese...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.02.006

    authors： Takahashi M,Takahashi S,Suzuki C,Jia L,Morimoto H,Ise H,Iwasaki T,Hattori H,Suzuki J,Miyamori I,Kobayashi E,Ikeda U

    更新日期：2005-04-01 00:00:00

  • Characterisation of murine cytomegalovirus myocarditis: cellular infiltration of the heart and virus persistence.

    abstract：:Myocarditis triggered by a viral infection has integral viral and immunological aspects associated with the pathogenesis of disease. The present study was performed to analyse the cellular inflammatory response in the heart and cytomegalovirus replication during the development of myocarditis in vivo. We examined muri...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2002.2003

    authors： Lenzo JC,Fairweather D,Cull V,Shellam GR,James Lawson CM

    更新日期：2002-06-01 00:00:00