Characterisation of murine cytomegalovirus myocarditis: cellular infiltration of the heart and virus persistence.


:Myocarditis triggered by a viral infection has integral viral and immunological aspects associated with the pathogenesis of disease. The present study was performed to analyse the cellular inflammatory response in the heart and cytomegalovirus replication during the development of myocarditis in vivo. We examined murine cytomegalovirus in an animal model of myocarditis using both susceptible BALB/c and resistant C57BL/6 mice. The heart infiltrating cells of BALB/c mice were found to comprise predominantly CD8+ T cells, with other cells of the CD4+ T cell, macrophage, B cell and neutrophil phenotype. Infectious MCMV titres in the heart were low and replicative virus could not be isolated beyond the first week post-infection (p.i.). Direct viral lysis of myocytes in vitro and apoptosis of cardiac cells in vivo was observed. Furthermore, viral DNA was detected in the heart of both mouse strains throughout the development of chronic disease. Viral gB RNA was detected during the first 35 days p.i. However, viral transcript for ie1 RNA but not gB RNA was found in the heart during the late stage of disease, suggesting latent viral infection of the heart. Our findings suggest that maintenance of the chronic phase of myocarditis involving post-viral immunological responses can occur in the presence of little infectious virus replication in the heart.


J Mol Cell Cardiol


Lenzo JC,Fairweather D,Cull V,Shellam GR,James Lawson CM





Has Abstract


2002-06-01 00:00:00














  • Reduced Ca(2+)-sensitivity of SERCA 2a in failing human myocardium due to reduced serin-16 phospholamban phosphorylation.

    abstract::It is still a matter of debate, whether decreased protein expression of SERCA 2a and phospholamban (PLB), or alterations in the phosphorylation state of PLB are responsible for the reduced SERCA 2a function in failing human myocardium. Thus, in membrane preparations from patients with terminal heart failure due to idi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Schwinger RH,Münch G,Bölck B,Karczewski P,Krause EG,Erdmann E

    更新日期:1999-03-01 00:00:00

  • The relationship between cardiac function and metabolism in acute adriamycin-treated perfused rat hearts studied by 31P and 13C NMR spectroscopy.

    abstract::Acute adriamycin cardiotoxicity was studied in the isolated, perfused rat heart by 31P and 13C NMR spectroscopy at flow rates of 15 and 5 ml/min. Treated hearts received a total dose of 13.5 mg of adriamycin. 31P NMR spectra were collected at the beginning and end of each experiment, and cardiac function was recorded ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Chatham JC,Cousins JP,Glickson JD

    更新日期:1990-10-01 00:00:00

  • S100A1 in cardiovascular health and disease: closing the gap between basic science and clinical therapy.

    abstract::Calcium (Ca(2+)) signaling plays a major role in a wide range of physiological functions including control and regulation of cardiac and skeletal muscle performance and vascular tone. As all Ca(2+) signals require proteins to relay intracellular Ca(2+) oscillations downstream to different signaling networks, a specifi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Kraus C,Rohde D,Weidenhammer C,Qiu G,Pleger ST,Voelkers M,Boerries M,Remppis A,Katus HA,Most P

    更新日期:2009-10-01 00:00:00

  • The SCFFBXO3 ubiquitin E3 ligase regulates inflammation in atherosclerosis.

    abstract::Inflammation is critical in the pathobiology of atherosclerosis. An essential player in the inflammatory process in atherosclerosis are macrophages that scavenge oxidatively modified low-density lipoproteins (OxLDL) deposited in the subendothelium of systemic arteries that secrete a myriad of pro-inflammatory mediator...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Chandra D,Londino J,Alexander S,Bednash JS,Zhang Y,Friedlander RM,Daskivich G,Carlisle DL,Lariviere WR,Nakassa ACI,Ross M,St Croix C,Nyunoya T,Sciurba F,Chen B,Mallampalli RK

    更新日期:2019-01-01 00:00:00

  • Effects of neuropeptide Y on collateral development in a swine model of chronic myocardial ischemia.

    abstract::We investigated the role of neuropeptide Y (NPY), abundant in the myocardial sympathetic nervous system and endothelial cells, in angiogenesis during chronic myocardial ischemia. Adult male Yorkshire swine underwent ameroid constrictor placement on the proximal left circumflex coronary artery. After 3 weeks, an osmoti...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Robich MP,Matyal R,Chu LM,Feng J,Xu SH,Laham RJ,Hess PE,Bianchi C,Sellke FW

    更新日期:2010-12-01 00:00:00

  • Fatty acid regulation of glucose metabolism in the intact beating rat heart assessed by carbon-13 NMR spectroscopy: the critical role of pyruvate dehydrogenase.

    abstract::Although the myocardium is capable of utilizing both glucose and fatty acid substrates, glucose metabolism is inhibited in the presence of fatty acid during normal perfusion conditions. Fatty acid regulation of glucose utilization in intact beating rat hearts was studied with 13C-enriched substrates and 13C and 31P NM...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Weiss RG,Chacko VP,Gerstenblith G

    更新日期:1989-05-01 00:00:00

  • Attenuation of myocardial ischaemic injury 24 h after diacylglycerol treatment in vivo.

    abstract::Previous work suggests that delayed protection against infarction following ischaemic preconditioning of rabbit myocardium may involve the activation of protein kinase C (PKC). Preconditioning in the presence of chelerythrine, an inhibitor of PKC, abolished the late anti-infarct effect of preconditioning. In the studi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Baxter GF,Mocanu MM,Yellon DM

    更新日期:1997-07-01 00:00:00

  • Cardiomyocyte specific overexpression of a 37 amino acid domain of regulator of G protein signalling 2 inhibits cardiac hypertrophy and improves function in response to pressure overload in mice.

    abstract::Regulator of G protein signalling 2 (RGS2) is known to play a protective role in maladaptive cardiac hypertrophy and heart failure via its ability to inhibit Gq- and Gs- mediated GPCR signalling. We previously demonstrated that RGS2 can also inhibit protein translation and can thereby attenuate cell growth. This G pro...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Lee KN,Lu X,Nguyen C,Feng Q,Chidiac P

    更新日期:2017-07-01 00:00:00

  • Proteotoxicity: an underappreciated pathology in cardiac disease.

    abstract::In general, in most organ systems, intracellular protein homeostasis is the sum of many factors, including chromosomal state, protein synthesis, post-translational processing and transport, folding, assembly and disassembly into macromolecular complexes, protein stability and clearance. In the heart, there has been a ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审


    authors: Sandri M,Robbins J

    更新日期:2014-06-01 00:00:00

  • Reperfusion-induced arrhythmias are critically dependent upon occluded zone size: relevance to the mechanism of arrhythmogenesis.

    abstract::The relationship between reperfusion-induced arrhythmias and the size of the occluded zone was examined. The isolated perfuse rat heart was used because its negligible collateral flow maximizes susceptibility to arrhythmias and reduces variability. Ischemia lasting 10 min was followed by 10 min of reperfusion. A const...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Curtis MJ,Hearse DJ

    更新日期:1989-06-01 00:00:00

  • Cardiomyocyte cell cycling, maturation, and growth by multinucleation in postnatal swine.

    abstract:BACKGROUND:Rodent cardiomyocytes (CM) undergo mitotic arrest and decline of mononucleated-diploid population post-birth, which are implicated in neonatal loss of heart regenerative potential. However, the dynamics of postnatal CM maturation are largely unknown in swine, despite a similar neonatal cardiac regenerative c...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Velayutham N,Alfieri CM,Agnew EJ,Riggs KW,Baker RS,Ponny SR,Zafar F,Yutzey KE

    更新日期:2020-09-01 00:00:00

  • Conditional knockout of Fgf13 in murine hearts increases arrhythmia susceptibility and reveals novel ion channel modulatory roles.

    abstract::The intracellular fibroblast growth factors (iFGF/FHFs) bind directly to cardiac voltage gated Na+ channels, and modulate their function. Mutations that affect iFGF/FHF-Na+ channel interaction are associated with arrhythmia syndromes. Although suspected to modulate other ionic currents, such as Ca2+ channels based on ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Wang X,Tang H,Wei EQ,Wang Z,Yang J,Yang R,Wang S,Zhang Y,Pitt GS,Zhang H,Wang C

    更新日期:2017-03-01 00:00:00

  • Adenosine and dipyridamole mimic the effects of ischemic preconditioning.

    abstract::The effects of preconditioning, adenosine and dipyridamole in protecting the systolic and diastolic alterations of myocardial stunning in rabbit hearts were studied. Isovolumic left ventricular developed pressure (LVDP), and end diastolic pressure (LVEDP) were measured. The time constant of relaxation (T) was calculat...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Mosca SM,Gelpi RJ,Cingolani HE

    更新日期:1994-10-01 00:00:00

  • Effect of ATP depletion on kinetics of Na/Ca exchange-mediated Ca influx in Na-loaded heart cells.

    abstract::The kinetics of Ca influx by Na/Ca exchange into adult rat heart cells loaded with Na and depleted of ATP were investigated, to further elucidate how ATP regulates exchanger properties in the intact heart cell. We found an eight-fold reduction in Vmax for Ca uptake by ATP depletion, with no significant change in the K...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Haworth RA,Biggs AV

    更新日期:1997-02-01 00:00:00

  • Effects of hemodynamic variables on myocardial K+ balance during and after shortlasting ischemia.

    abstract::Ischemia-induced myocardial potassium loss and post-ischemic potassium reuptake was quantitated in 8 open chest pigs during control conditions and during hemodynamic alterations which have been shown to increase steady state sarcolemmal potassium fluxes. Myocardial K+ balance was continuously computed before, during a...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Aksnes G,Ellingsen O,Rutlen DL,Ilebekk A

    更新日期:1989-12-01 00:00:00

  • Molecular nature and regulation of the mitochondrial permeability transition pore(s), drug target(s) in cardioprotection.

    abstract::The mitochondrial permeability transition, an established mechanism for heart diseases, is a long-standing mystery of mitochondrial biology and a prime drug target for cardioprotection. Several hypotheses about its molecular nature have been put forward over the years, and the prevailing view is that permeabilization ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Carraro M,Carrer A,Urbani A,Bernardi P

    更新日期:2020-07-01 00:00:00

  • Depressed Frank-Starling mechanism in the left ventricular muscle of the knock-in mouse model of dilated cardiomyopathy with troponin T deletion mutation ΔK210.

    abstract::It has been reported that the Frank-Starling mechanism is coordinately regulated in cardiac muscle via thin filament "on-off" equilibrium and titin-based lattice spacing changes. In the present study, we tested the hypothesis that the deletion mutation ΔK210 in the cardiac troponin T gene shifts the equilibrium toward...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Inoue T,Kobirumaki-Shimozawa F,Kagemoto T,Fujii T,Terui T,Kusakari Y,Hongo K,Morimoto S,Ohtsuki I,Hashimoto K,Fukuda N

    更新日期:2013-10-01 00:00:00

  • Rational design of a selective antagonist of epsilon protein kinase C derived from the selective allosteric agonist, pseudo-RACK peptide.

    abstract::We have previously shown that domains involved in binding of protein kinase C (PKC) isozymes to their respective anchoring proteins (RACKs) and short peptides derived from these domains are PKC isozyme-selective antagonists. We also identified PKC isozyme-selective agonists, named psiRACK peptides, derived from a sequ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Liron T,Chen LE,Khaner H,Vallentin A,Mochly-Rosen D

    更新日期:2007-04-01 00:00:00

  • Quantifying antiarrhythmic drug blocking during action potentials in guinea-pig papillary muscle.

    abstract::This report examines the blocking effects that several antiarrhythmic drugs have on rapid upstroke velocities of action potentials recorded from guinea-pig papillary muscle. A mathematical analysis is developed, that measures an effective "blocking rate' that acts during the action potential with the purpose being to ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Courtney KR

    更新日期:1983-11-01 00:00:00

  • Expression of perlecan proteoglycan in the infarct zone of mouse myocardial infarction.

    abstract::Perlecan, a basal lamina proteoglycan, has been shown to interact with other extracellular matrix (ECM) components, especially type IV collagen, and is thus involved in ECM formation. Perlecan has also been postulated to promote growth factor-receptor interactions, including the binding of basic fibroblast growth fact...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Nakahama M,Murakami T,Kusachi S,Naito I,Takeda K,Ohnishi H,Komatsubara I,Oka T,Ninomiya Y,Tsuji T

    更新日期:2000-06-01 00:00:00

  • Phospholamban pentamers attenuate PKA-dependent phosphorylation of monomers.

    abstract::Phospholamban (PLN) is a key regulator of cardiac contraction and relaxation through its inhibition of the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA2a). The inhibitory effect is attenuated upon protein kinase A (PKA) dependent phosphorylation of PLN. PLN exists in an equilibrium of pentamers and monomers. While...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Wittmann T,Lohse MJ,Schmitt JP

    更新日期:2015-03-01 00:00:00

  • Maturational differences in bioenergetic state and purine formation during "supply" and "demand" ischemia.

    abstract::We examined metabolic effects of "supply" and "demand" ischemia in immature and mature rabbit hearts. Moderate supply ischemia was produced by a 50% reduction in coronary flow (to approximately 5.0 ml min-1 g-1 giving a 50-55% rise in O2 extraction and a 35% drop in O2 supply/demand). Demand ischemia was produced by s...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Matherne GP,Ely SW,Headrick JP

    更新日期:1996-05-01 00:00:00

  • Novel C-terminus frameshift mutation, 1122fs/147, of HERG in LQT2: additional amino acids generated by frameshift cause accelerated inactivation.

    abstract:OBJECTIVE:The function of the C-terminus region of the human ether-a-go-go related gene (HERG) has not been well characterized except for its involvement in trafficking. To understand further the role of C-terminus region, we performed a functional analysis of a novel frameshift mutation (1122fs/147) identified in a Ja...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Sasano T,Ueda K,Orikabe M,Hirano Y,Kawano S,Yasunami M,Isobe M,Kimura A,Hiraoka M

    更新日期:2004-12-01 00:00:00

  • Functional importance of cardiac enhancer-associated noncoding RNAs in heart development and disease.

    abstract::The key information processing units within gene regulatory networks are enhancers. Enhancer activity is associated with the production of tissue-specific noncoding RNAs, yet the existence of such transcripts during cardiac development has not been established. Using an integrated genomic approach, we demonstrate that...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Ounzain S,Pezzuto I,Micheletti R,Burdet F,Sheta R,Nemir M,Gonzales C,Sarre A,Alexanian M,Blow MJ,May D,Johnson R,Dauvillier J,Pennacchio LA,Pedrazzini T

    更新日期:2014-11-01 00:00:00

  • Cardiac morphogenetic defects and conduction abnormalities in mice homozygously deficient for connexin40 and heterozygously deficient for connexin45.

    abstract::Connexin40 (Cx40) and connexin45 (Cx45) are involved in both cardiac morphogenesis and propagation of electrical activity. We found that Cx40/Cx45 double deficiency (Cx40(-/-)/Cx45(+/-)) causes a variety of cardiac defects leading to high mortality during embryonic development and at birth. The majority of Cx40(-/-)/C...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Krüger O,Maxeiner S,Kim JS,van Rijen HV,de Bakker JM,Eckardt D,Tiemann K,Lewalter T,Ghanem A,Lüderitz B,Willecke K

    更新日期:2006-11-01 00:00:00

  • Regulation of the endothelin-1 transmembrane signaling pathway: the potential role of agonist-induced desensitization in the coronary artery of the rapid ventricular pacing-overdrive dog model of heart failure.

    abstract::This study examined the potential role of ET-1 and the contribution of protein kinase C (PKC) in the desensitization of the ET-1 transmembrane signaling pathway in the left circumflex coronary artery (CCA) of a dog model of congestive heart failure (CHF). In the CCA of the rapid ventricular pacing-overdrive dog model ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Calderone A,Rouleau JL,de Champlain J,Bélichard P,Stewart DJ

    更新日期:1993-08-01 00:00:00

  • Stimulation of DNA synthesis by PDGF in the newt cardiac myocyte.

    abstract::The adult newt cardiac ventricular myocyte has been successfully placed in cell culture and has been shown to undergo in vitro DNA synthesis. Although several growth factors have been reported to increase DNA synthesis in cardiac myocytes in vitro, PDGF has not been reported to do so, but has been shown to be active i...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Soonpaa MH,Oberpriller JO,Oberpriller JC

    更新日期:1992-09-01 00:00:00

  • Taurine depletion caused by knocking out the taurine transporter gene leads to cardiomyopathy with cardiac atrophy.

    abstract::The sulfur-containing beta-amino acid, taurine, is the most abundant free amino acid in cardiac and skeletal muscle. Although its physiological function has not been established, it is thought to play an important role in ion movement, calcium handling, osmoregulation and cytoprotection. To begin examining the physiol...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Ito T,Kimura Y,Uozumi Y,Takai M,Muraoka S,Matsuda T,Ueki K,Yoshiyama M,Ikawa M,Okabe M,Schaffer SW,Fujio Y,Azuma J

    更新日期:2008-05-01 00:00:00

  • Adenosine receptor blockade enhances isoproterenol-induced increases in cardiac interstitial adenosine.

    abstract::The failure of adenosine receptor antagonists to consistently attenuate metabolic coronary vasodilation suggests that adenosine is not a primary regulator of functional hyperemia. An alternative hypothesis, however, is that metabolic stimulation of the heart in the presence of an adenosine receptor antagonist results ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Heller LJ,Dole WP,Mohrman DE

    更新日期:1991-08-01 00:00:00

  • Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.

    abstract::We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals we...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章


    authors: Eschenhagen T,Laufs U,Schmitz W,Scholz H,Warnholtz A,Weil J,Schäfer HJ

    更新日期:1995-10-01 00:00:00