Characterisation of murine cytomegalovirus myocarditis: cellular infiltration of the heart and virus persistence.

Abstract:

:Myocarditis triggered by a viral infection has integral viral and immunological aspects associated with the pathogenesis of disease. The present study was performed to analyse the cellular inflammatory response in the heart and cytomegalovirus replication during the development of myocarditis in vivo. We examined murine cytomegalovirus in an animal model of myocarditis using both susceptible BALB/c and resistant C57BL/6 mice. The heart infiltrating cells of BALB/c mice were found to comprise predominantly CD8+ T cells, with other cells of the CD4+ T cell, macrophage, B cell and neutrophil phenotype. Infectious MCMV titres in the heart were low and replicative virus could not be isolated beyond the first week post-infection (p.i.). Direct viral lysis of myocytes in vitro and apoptosis of cardiac cells in vivo was observed. Furthermore, viral DNA was detected in the heart of both mouse strains throughout the development of chronic disease. Viral gB RNA was detected during the first 35 days p.i. However, viral transcript for ie1 RNA but not gB RNA was found in the heart during the late stage of disease, suggesting latent viral infection of the heart. Our findings suggest that maintenance of the chronic phase of myocarditis involving post-viral immunological responses can occur in the presence of little infectious virus replication in the heart.

journal_name

J Mol Cell Cardiol

authors

Lenzo JC,Fairweather D,Cull V,Shellam GR,James Lawson CM

doi

10.1006/jmcc.2002.2003

keywords:

subject

Has Abstract

pub_date

2002-06-01 00:00:00

pages

629-40

issue

6

eissn

0022-2828

issn

1095-8584

pii

S0022282802920031

journal_volume

34

pub_type

杂志文章
  • Epithelial-mesenchymal transition of epicardial mesothelium is a source of cardiac CD117-positive stem cells in adult human heart.

    abstract::Epithelial-mesenchymal transition is implicated in the remodelling of tissues during development and in the adult life. In the heart, it gives origin to progenitors of fibroblasts, coronary endothelium, smooth muscle cells, and cardiomyocytes. Moreover, epicardially-derived cells determine myocardial wall thickness an...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2010.05.013

    authors: Di Meglio F,Castaldo C,Nurzynska D,Romano V,Miraglia R,Bancone C,Langella G,Vosa C,Montagnani S

    更新日期:2010-11-01 00:00:00

  • Prolyl hydroxylation regulates intracellular procollagen degradation in cultured rat cardiac fibroblasts.

    abstract::To determine the regulatory role of prolyl hydroxylation in intracellular cardiac procollagen turnover, we examined the effects of prolyl 4-hydroxylase inhibitors (alpha, alpha-dipyridil, 3,4-dihydroxybenzoic acid ethyl ester, pyridine 2,4-dicarboxylic acid ethyl ester) and ascorbic acid on procollagen metabolism by c...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/s0022-2828(95)90095-0

    authors: Eleftheriades EG,Ferguson AG,Spragia ML,Samarel AM

    更新日期:1995-08-01 00:00:00

  • The calcium paradox phenomenon: a flow rate and volume response study of calcium-free perfusion.

    abstract::A dose-response study concerning the importance of the flow rate (0.5 to 12 ml/min) and volume (2.5 to 60 ml) of calcium-free coronary perfusion (duration 5 min) in the induction of a calcium paradox on reperfusion (duration 15 min) with calcium-containing medium has been performed in the isolated rat heart (37 degree...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/s0022-2828(85)80076-6

    authors: Oksendal AN,Jynge P,Sellevold OF,Rotevatn S,Saetersdal T

    更新日期:1985-10-01 00:00:00

  • Development of novel method of non-viral efficient gene transfer into neonatal cardiac myocytes.

    abstract::To establish new treatment for cardiovascular disease, the development of safe and highly efficient vectors is necessary. Especially, non-viral vectors are considered to be ideal for human gene therapy, since recent adverse events with retroviral or adenoviral vectors have highlighted the issue of safety. Although we ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2005.06.007

    authors: Tashiro H,Aoki M,Isobe M,Hashiya N,Makino H,Kaneda Y,Ogihara T,Morishita R

    更新日期:2005-09-01 00:00:00

  • Distribution of the neuropeptide galanin in the cat heart and coexistence with vasoactive intestinal peptide, substance P and neuropeptide Y.

    abstract::The neuropeptide galanin (GAL) has been detected in the peripheral and central nervous systems. However, little is known about its distribution and localization in heart, and the possible coexistence of GAL with other neuropeptides in the heart is not established. The present immunocytochemical study describes the dis...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/0022-2828(92)91157-z

    authors: Zhu W,Dey RD

    更新日期:1992-01-01 00:00:00

  • Chronic β-adrenergic stimulation reverses depressed Ca handling in mice overexpressing inhibitor-2 of protein phosphatase 1.

    abstract:RATIONALE:A higher expression/activity of type 1 serine/threonine protein phosphatase 1 (PP1) may contribute to dephosphorylation of cardiac regulatory proteins triggering the development of heart failure. OBJECTIVE:Here, we tested the putatively protective effects of PP1 inhibitor-2 (I2) overexpression using a heart ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2018.10.022

    authors: Kirchhefer U,Hammer E,Heinick A,Herpertz T,Isensee G,Müller FU,Neumann J,Schulte K,Seidl MD,Boknik P,Schulte JS

    更新日期:2018-12-01 00:00:00

  • NHE-1 inhibition improves impaired mitochondrial permeability transition and respiratory function during postinfarction remodelling in the rat.

    abstract::The mechanism by which Na+-H+ exchange (NHE) inhibition results in attenuation and reversal of postinfarction remodelling and heart failure remains controversial. In this study, we investigated the possible contribution of mitochondrial involvement by determining the effect of the NHE-1-specific inhibitor EMD-87580 (E...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2004.10.007

    authors: Javadov S,Huang C,Kirshenbaum L,Karmazyn M

    更新日期:2005-01-01 00:00:00

  • Some biochemical aspects of the protective effect of trimetazidine on rat cardiomyocytes during hypoxia and reoxygenation.

    abstract::This study was undertaken to evaluate the direct cardioprotective effect of trimetazidine (TMZ), an anti-anginal drug devoid of haemodynamic action, on isolated myocytes. Cultured rat ventricular myocytes were treated with the drug 16 h and 1 h before the experiments. The drug-treated cells and control cells were plac...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.1994.1116

    authors: Fantini E,Demaison L,Sentex E,Grynberg A,Athias P

    更新日期:1994-08-01 00:00:00

  • Characterization of contractile function in diabetic hypertensive cardiomyopathy in adult rat ventricular myocytes.

    abstract::Diabetes and hypertension both produce myocardial dysfunction that accelerates cardiovascular morbidity and mortality. Coexistence of the two often results in a more severe cardiomyopathy than either process alone. The purpose of this study was to characterize the contractile function of diabetic hypertensive cardiomy...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.2001.1431

    authors: Wold LE,Relling DP,Colligan PB,Scott GI,Hintz KK,Ren BH,Epstein PN,Ren J

    更新日期:2001-09-01 00:00:00

  • Characterization of [3H]U69593 binding sites in the rat heart by receptor binding assays.

    abstract::The binding properties of [3H]U69593, a selective k-ligand, in rat heart homogenates were characterized by direct and displacement receptor binding assays. It was found that there are substantial specific [3H]U69593 binding sites in the rat heart. They were saturable, reversible and stereospecific. Both association an...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/0022-2828(91)90086-2

    authors: Tai KK,Jin WQ,Chan TK,Wong TM

    更新日期:1991-11-01 00:00:00

  • Interaction between propranolol and calcium channel blockers in cardiac and vascular smooth muscle.

    abstract::The ability of the beta-receptor antagonist propranolol to influence the response of isolated cardiac and vascular smooth muscle to several classes of calcium channel blockers was examined. For comparison, the interactions between propranolol and other classes of negative inotropic and vasorelaxant agents was also eva...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/s0022-2828(88)80144-5

    authors: Weishaar RE,Quade MM,Pugsley TA,Shih YH,Taylor DG Jr

    更新日期:1988-10-01 00:00:00

  • Ex vivo expanded hematopoietic progenitor cells improve cardiac function after myocardial infarction: role of beta-catenin transduction and cell dose.

    abstract::Cell-based therapy after myocardial infarction (MI) is a promising therapeutic option but the relevant cell subsets and dosage requirements are poorly defined. We hypothesized that cell therapy for myocardial infarction is improved by ex vivo expansion and high-dose transplantation of defined hematopoietic progenitor ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2008.06.010

    authors: Templin C,Kotlarz D,Faulhaber J,Schnabel S,Grote K,Salguero G,Luchtefeld M,Hiller KH,Jakob P,Naim HY,Schieffer B,Hilfiker-Kleiner D,Landmesser U,Limbourg FP,Drexler H

    更新日期:2008-09-01 00:00:00

  • Heat shock protein upregulation protects against pacing-induced myolysis in HL-1 atrial myocytes and in human atrial fibrillation.

    abstract::Atrial fibrillation (AF) causes myocyte stress by inducing structural changes, predominantly myolysis, which is related to the progression of AF. As heat shock proteins (Hsp) protect against cellular stress, their efficacy in preventing myolysis was investigated in a tachy-paced cell model for AF and in patients with ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2006.06.068

    authors: Brundel BJ,Henning RH,Ke L,van Gelder IC,Crijns HJ,Kampinga HH

    更新日期:2006-09-01 00:00:00

  • Effects of trimetazidine on action potentials and membrane currents of guinea-pig ventricular myocytes.

    abstract::The effects of trimetazidine on membrane potentials and membrane currents of enzymatically isolated guinea-pig ventricular cells were studied with the use of giga-seal suction pipettes for patch clamp. Trimetazidine (3 X 10(-5) M) decreased the action potential duration from 433 +/- 179 ms (mean and S.D., n = 9) to 31...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/s0022-2828(86)80433-3

    authors: Kiyosue T,Nakamura S,Arita M

    更新日期:1986-12-01 00:00:00

  • Calpain mediates cardiac troponin degradation and contractile dysfunction in atrial fibrillation.

    abstract::The self-perpetuation of atrial fibrillation (AF) is associated with atrial remodeling, including the degradation of the myofibril structure (myolysis). Myolysis is related to AF-induced activation of cysteine proteases and underlies loss of contractile function. In this study, we investigated which proteases are invo...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2008.08.012

    authors: Ke L,Qi XY,Dijkhuis AJ,Chartier D,Nattel S,Henning RH,Kampinga HH,Brundel BJ

    更新日期:2008-11-01 00:00:00

  • Extracellular matrix remodeling in atrial fibrosis: mechanisms and implications in atrial fibrillation.

    abstract::Atrial fibrosis has been strongly associated with the presence of heart diseases/arrhythmias, including congestive heart failure (CHF) and atrial fibrillation (AF). Inducibility of AF as a result of atrial fibrosis has been the subject of intense recent investigation since it is the most commonly encountered arrhythmi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.yjmcc.2009.09.001

    authors: Pellman J,Lyon RC,Sheikh F

    更新日期:2010-03-01 00:00:00

  • Ischemic preconditioning: a potential role for constitutive low molecular weight stress protein translocation and phosphorylation?

    abstract::We have investigated whether translocation of constitutive low molecular weight stress proteins (alphaB-crystallin and HSP27) to the myofilament/cytoskeletal compartment occurs during ischemic preconditioning and assessed if this is causally associated with cardioprotection. Triton-insoluble preparations from fresh or...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.2000.1136

    authors: Eaton P,Awad WI,Miller JI,Hearse DJ,Shattock MJ

    更新日期:2000-06-01 00:00:00

  • Regional expression of protein phosphatase type 1 and 2A catalytic subunit isoforms in the human heart.

    abstract::In mammalian species, including man, the duration of myocardial contraction is shorter in atria than ventricles. Total contraction time depends at least in part on phosphorylation and dephosphorylation of cardiac regulatory proteins. Dephosphorylation reactions are mediated by protein phosphatases. In the mammalian he...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.2000.1265

    authors: Lüss H,Klein-Wiele O,Bokník P,Herzig S,Knapp J,Linck B,Müller FU,Scheld HH,Schmid C,Schmitz W,Neumann J

    更新日期:2000-12-01 00:00:00

  • An analysis of lidocaine block of sodium current in isolated human atrial and ventricular myocytes.

    abstract::Lidocaine is a Na+ channel blocker that is highly effective for the treatment of ventricular tachyarrhythmias, but is largely ineffective against atrial arrhythmias. If is not known if this differential efficacy is the result of differences in lidocaine inhibition of atrial v ventricular Na+ channels. The purpose of t...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/0022-2828(95)90090-x

    authors: Furukawa T,Koumi S,Sakakibara Y,Singer DH,Jia H,Arentzen CE,Backer CL,Wasserstrom JA

    更新日期:1995-02-01 00:00:00

  • Regional biochemical remodeling in non-infarcted tissue of rat heart post-myocardial infarction.

    abstract::Changes in the capacities of ATP-synthesizing reactions were analysed in residual non-infarcted myocardium following myocardial infarction. Rats were subjected to left coronary artery ligation (MI; n = 11) or to sham operation (sham; n = 18). Two months later, hearts were excised, rinsed and buffer-perfused isovolumic...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.1996.0143

    authors: Laser A,Ingwall JS,Tian R,Reis I,Hu K,Gaudron P,Ertl G,Neubauer S

    更新日期:1996-07-01 00:00:00

  • Viral expression of a SERCA2a-activating PLB mutant improves calcium cycling and synchronicity in dilated cardiomyopathic hiPSC-CMs.

    abstract::There is increasing momentum toward the development of gene therapy for heart failure (HF) that is defined by impaired calcium (Ca2+) transport and reduced contractility. We have used FRET (fluorescence resonance energy transfer) between fluorescently-tagged SERCA2a (the cardiac Ca2+ pump) and PLB (phospholamban, vent...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2019.11.147

    authors: Stroik DR,Ceholski DK,Bidwell PA,Mleczko J,Thanel PF,Kamdar F,Autry JM,Cornea RL,Thomas DD

    更新日期:2020-01-01 00:00:00

  • Unique single molecule binding of cardiac myosin binding protein-C to actin and phosphorylation-dependent inhibition of actomyosin motility requires 17 amino acids of the motif domain.

    abstract::Cardiac myosin binding protein-C (cMyBP-C) has 11 immunoglobulin or fibronectin-like domains, C0 through C10, which bind sarcomeric proteins, including titin, myosin and actin. Using bacterial expressed mouse N-terminal fragments (C0 through C3) in an in vitro motility assay of myosin-generated actin movement and the ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2011.09.019

    authors: Weith A,Sadayappan S,Gulick J,Previs MJ,Vanburen P,Robbins J,Warshaw DM

    更新日期:2012-01-01 00:00:00

  • Improvement of cholera toxin-catalyzed ADP-ribosylation by endogenous ADP-ribosylation factor from bovine brain provides evidence for an unchanged amount of Gs alpha in failing human myocardium.

    abstract::The aim of the present study was to investigate whether or not alterations of Gs alpha can be detected with cholera toxin-induced ADP-ribosylation in myocardial membranes from patients with heart failure. Therefore, Gs alpha was radiolabeled by cholera toxin-catalzyed (32P)ADP-ribosylation with (32P)NAD as substrate. ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/0022-2828(90)90973-6

    authors: Schnabel P,Böhm M,Gierschik P,Jakobs KH,Erdmann E

    更新日期:1990-01-01 00:00:00

  • Mitochondria-targeted ROS scavenger improves post-ischemic recovery of cardiac function and attenuates mitochondrial abnormalities in aged rats.

    abstract::Mitochondria-generated reactive oxygen species (ROS) play a crucial role in the pathogenesis of aging and age-associated diseases. In this study, we evaluated the effects of XJB-5-131 (XJB), a mitochondria-targeted ROS and electron scavenger, on cardiac resistance to ischemia-reperfusion (IR)-induced oxidative stress ...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2014.10.009

    authors: Escobales N,Nuñez RE,Jang S,Parodi-Rullan R,Ayala-Peña S,Sacher JR,Skoda EM,Wipf P,Frontera W,Javadov S

    更新日期:2014-12-01 00:00:00

  • Restoration of mechanical and energetic function in failing aortic-banded rat hearts by gene transfer of calcium cycling proteins.

    abstract::The aim of this study was to examine whether short- and long-term gene transfer of Ca(2+) handling proteins restore left ventricular (LV) mechanoenergetics in aortic banding-induced failing hearts. Aortic-banded rats received recombinant adenoviruses carrying sarcoplasmic reticulum Ca(2+)-ATPase (SERCA2a) (Banding+SER...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2007.01.003

    authors: Sakata S,Lebeche D,Sakata N,Sakata Y,Chemaly ER,Liang LF,Tsuji T,Takewa Y,del Monte F,Peluso R,Zsebo K,Jeong D,Park WJ,Kawase Y,Hajjar RJ

    更新日期:2007-04-01 00:00:00

  • Fibroblast activation protein alpha expression identifies activated fibroblasts after myocardial infarction.

    abstract:INTRODUCTION:Fibroblast activation protein α (FAP) is a membrane-bound serine protease expressed by activated fibroblasts during wound healing in the skin. Expression of FAP after myocardial infarction (MI) and potential effects on cardiac wound healing are largely unknown. METHODS:MI was induced in rats and FAP expre...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2015.08.016

    authors: Tillmanns J,Hoffmann D,Habbaba Y,Schmitto JD,Sedding D,Fraccarollo D,Galuppo P,Bauersachs J

    更新日期:2015-10-01 00:00:00

  • Expression and cellular distribution of alpha(v)integrins in beta(1)integrin-deficient embryonic stem cell-derived cardiac cells.

    abstract::beta(1)integrin-deficient (beta(1)-/-) ES cells showed increased differentiation of cardiac cells characterized by reduced adhesion and high beating frequency. Whereas in whole embryoid body outgrowths of beta(1)-/- cells maximum levels of alpha(v), beta(3)and beta(5)integrin mRNA were delayed and transiently upregula...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.2000.1326

    authors: Guan K,Czyz J,Fürst DO,Wobus AM

    更新日期:2001-03-01 00:00:00

  • Mitochondrial adaptations within chronically ischemic swine myocardium.

    abstract::Experimental evidence has emerged that myocardial ischemic preconditioning can prime the mitochondria into a "stress-resistant state", so that cell death is reduced following prolonged severe ischemia and reperfusion. Using a swine model of chronically ischemic myocardium, we tested the hypothesis that mitochondria wi...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2006.07.008

    authors: McFalls EO,Sluiter W,Schoonderwoerd K,Manintveld OC,Lamers JM,Bezstarosti K,van Beusekom HM,Sikora J,Ward HB,Merkus D,Duncker DJ

    更新日期:2006-12-01 00:00:00

  • Novel C-terminus frameshift mutation, 1122fs/147, of HERG in LQT2: additional amino acids generated by frameshift cause accelerated inactivation.

    abstract:OBJECTIVE:The function of the C-terminus region of the human ether-a-go-go related gene (HERG) has not been well characterized except for its involvement in trafficking. To understand further the role of C-terminus region, we performed a functional analysis of a novel frameshift mutation (1122fs/147) identified in a Ja...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1016/j.yjmcc.2004.09.010

    authors: Sasano T,Ueda K,Orikabe M,Hirano Y,Kawano S,Yasunami M,Isobe M,Kimura A,Hiraoka M

    更新日期:2004-12-01 00:00:00

  • Improved functional recovery of ischemic rat hearts due to singlet oxygen scavengers histidine and carnosine.

    abstract::There is increasing evidence that reactive oxygen species (ROS) contribute to post-ischemic reperfusion injury, but determination of the specific ROS involved has proven elusive. In the present study electron paramagnetic resonance (EPR) spectroscopy was used in vitro to measure the relative quenching of singlet oxyge...

    journal_title:Journal of molecular and cellular cardiology

    pub_type: 杂志文章

    doi:10.1006/jmcc.1998.0850

    authors: Lee JW,Miyawaki H,Bobst EV,Hester JD,Ashraf M,Bobst AM

    更新日期:1999-01-01 00:00:00