Abstract：
:Objective: Both previous and recent literature showed long non-coding RNAs (lncRNAs) were crucial participants in multiple myeloma (MM) evolution. However, the dynamic regulation and mechanism of lncRNAs in MM progression was not fully understood. This study will explore the expression and effects of prostate cancer-associated ncRNA transcript 1 (PCAT-1) in MM. Materials and Methods: The expression level of PCAT-1 was examined using quantitative real-time PCR in patients with newly diagnosed MM and cell lines. The potential biological effects and molecular mechanisms of PCAT-1 in MM were evaluated using a series of soft agar colony formation assay, CCK-8 assay, cell cycle and apoptosis assay by flow cytometry, protein chip arrays, western blot analysis, immunohistochemistry and nude subcutaneous tumorigenesis model. Results: High expression of PCAT-1 was observed in patients with newly diagnosed MM and cell lines. Over-expressed PCAT-1 enhanced cell division and inhibited apoptosis both in cultured cells and in animal model. Meanwhile, silenced PCAT-1 exerted the opposite function. Additionally, PCAT-1 knockdown sensitized MM cells to bortezomib (Bort). Inhibitor of PCAT-1 combination with Bort exhibited a more effective inhibitory effect on MM cells compared with negative control or Bort alone. Further mechanism exploration via protein chips, Go and KEGG pathway analysis along with immunoblot analysis revealed that PCAT-1 facilitated cell growth and drug resistance via the p38 and JNK MAPK pathways. Conclusion: This study identified a novel lncRNA-associated mechanism underlying MM carcinogenesis, and provided clinicians with a promising therapeutic target in MM.
journal_name
J Cancerjournal_title
Journal of Cancerauthors
Shen X,Shen P,Yang Q,Yin Q,Wang F,Cong H,Wang X,Ju Sdoi
10.7150/jca.35098subject
Has Abstractpub_date
2019-10-20 00:00:00pages
6502-6510issue
26issn
1837-9664pii
jcav10p6502journal_volume
10pub_type
杂志文章abstract：:The impact of albumin to globulin ratio (AGR) on the prognosis of various human cancers has not been well established. Here, a systemic review and meta-analysis has been performed to comprehensively assess the relationships between AGR and lymph node metastasis (LNM) or overall survival (OS). Systematical search throu...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.24889
更新日期：2018-06-12 00:00:00
abstract：:Background. Esophageal cancer is ranked in the top ten of diagnosed tumors worldwide. Even though improvements in survival could be noticed over the last years, prognosis remains poor. ETS translocation variant 1 (ETV1) is a member of a family of transcription factors and is phosphorylated by mitogen-activated protein...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.22310
更新日期：2018-01-01 00:00:00
abstract：:Objective: Emerging evidence suggests that calreticulin (CALR) has great impacts on the tumor formation and progression of various cancers, but the role of CALR remains controversial. We investigated the expression and clinical significance of CALR in nasopharyngeal carcinoma (NPC). Methods: Immunohistochemistry was u...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.35362
更新日期：2019-08-29 00:00:00
abstract：:Purpose: Eukaryotic initiation factor 4A-3 (EIF4A3) is an RNA-binding protein (RBP) that is a core component of the exon junction complex (EJC). It has been identified as an important player in post-transcriptional regulation processes. Recently, investigations have focused on EIF4A3 dysfunction in carcinogenesis. The...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.27655
更新日期：2018-11-11 00:00:00
abstract：:Tumor progression is often associated with chronic inflammation in the tumor microenvironment, which is mediated by numerous cytokines, chemokines and growth factors produced by cancer and stroma cells. All these mediators support tumor development and immunosuppression in autocrine and/or paracrine ways. Neutralizati...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.5047
更新日期：2013-01-01 00:00:00
abstract：:Background: Population-based data on the prognosis of brain metastases at initial diagnosis of ovary cancer (OCBM) are currently lacking. Besides, the effective treatment for OCBM patients is still controversial now. The study aimed to explore the prognostic factors among OCBM. Methods: We retrospectively reviewed the...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.44494
更新日期：2020-05-18 00:00:00
abstract：:Colorectal cancer (CRC) is the fifth leading cause of cancer-related death in China. The incidence of Chinese CRC has increased dramatically with the changes of dietary and lifestyle. However, the genetic landscape of Chinese colorectal cancer mutation is still poorly understood. In this study, we have performed whole...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.37017
更新日期：2020-01-01 00:00:00
abstract：:We examined the feasibility and safety of using paclitaxel and trastuzumab as maintenance therapy after high-dose chemotherapy (HDC) with autologous hematopoietic stem cell transplantation (AHST) for patients with HER2-positive metastatic breast cancer. Ten patients (9 women and 1 man) were enrolled in the study. The ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.6775
更新日期：2013-09-27 00:00:00
abstract：:Background & Aims: Primary tumors of colorectal carcinoma (CRC) with liver metastasis might gain some liver-specific characteristics to adapt the liver micro-environment. This study aims to reveal potential liver-like transcriptional characteristics associated with the liver metastasis in primary colorectal carcinoma....
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.23017
更新日期：2018-04-06 00:00:00
abstract：:Galectins are the member of soluble proteins that bind with β-galactoside containing glycans. These proteins have been considered to be associated in various important events such as different types of cancers. It has been found that galectins could contribute to neoplastic transformation or regulate cell growth, cell...
journal_title：Journal of Cancer
pub_type： 杂志文章,评审
doi：10.7150/jca.23628
更新日期：2018-11-25 00:00:00
abstract：:Purpose: The objective of the study was to evaluate the important role played by androgen and insulin in the development of endometrial carcinoma (EC), and their combined effect on EC risk. Methods: We enrolled 510 type I EC patients and 510 age-, time-, and nationality-matched subjects into this study. Metabolic and ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.46391
更新日期：2020-07-25 00:00:00
abstract：:Background: Given recent results indicating that diminished LKB1 expression in laryngeal cancer correlates with shorter survival. We aim to perform an analysis estimate the role of decreased liver kinase B1(LKB1) and in the prognostication of human laryngeal squamous cell carcinoma (LSCC). Methods: We conducted a retr...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.20535
更新日期：2017-09-30 00:00:00
abstract：:Background: Anti-EGFR therapies have been recommended for advanced colorectal cancer (CRC) with wild-type RAS and PIK3CA mutation. However, PIK3CA mutations are a poor prognostic marker and a negative predictor of response to anti-EGFR therapies in RAS wild-type CRC. Therefore, new and advanced treatment strategies ar...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.19458
更新日期：2017-08-22 00:00:00
abstract：:Background: While curative resection is the established strategy for Hepatocellular carcinoma (HCC) patients, the prognosis still remains poor, and the efficiency of existing prediction models is unsatisfactory. Therefore, we aimed to develop a credible and easy-to-use prognostic index for patients with HCC undergoing...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.22246
更新日期：2018-01-01 00:00:00
abstract：:Objectives: To investigate the role of inflammation-related factors, lymphocyte-to-monocyte ratio (LMR) alone and combined detection with cancer antigen 125 (CA125), in the prognostic assessment of ovarian cancer (OC). Methods: A retrospective clinicopathologic review was performed. The receiver-operating characterist...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.42477
更新日期：2020-03-04 00:00:00
abstract：:Cisplatin chemoresistance is a clinical obstacle in the treatment of gastric cancer (GC). Enhanced DNA repair capacity may lead to cisplatin resistance. However, the detailed molecular mechanism of GC cisplatin resistance specifically involving nucleotide excision repair (NER) is not clear. However, the mechanism thro...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.25814
更新日期：2019-01-29 00:00:00
abstract：:Background: To investigate the relationship between clinical and histopathological characteristics and overall survival of patients with oral mucosal melanoma (OMM) without distal metastasis in order to provide predictive prognostic information of OMM. Methods: Ki67 expression was assessed by immunohistochemistry in 1...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.20935
更新日期：2017-10-17 00:00:00
abstract：BACKGROUND:This study was performed to assess the impact of irradiation on the tissue penetration depth of doxorubicin delivered during Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC). METHODS:Fresh post mortem swine peritoneum was cut into 10 proportional sections. Except for 2 control samples, all received...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.14714
更新日期：2016-05-07 00:00:00
abstract：:Background: Carbonic anhydrase 4 (CA4) maintains homeostasis of carbon dioxide and bicarbonate. It is suggested to be a potential prognostic biomarker, while the correlations between CA4 and different cancers are indistinct. Methods: Differential mRNA expression of CA4 among different cancers and corresponding normal ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.46902
更新日期：2020-08-25 00:00:00
abstract：:Background: The study of CTLA-4 inhibitors has been one of the hot spots in the field of tumor immunotherapy. As the most immunogenic subtype of breast cancer, Triple negative breast cancer (TNBC) has a great potential in the treatment strategy. The aim of this study was to explore the relevant genes and pathways of C...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.46301
更新日期：2020-09-09 00:00:00
abstract：:Philadelphia chromosome-positive (Ph+) leukemia is characterized by reciprocal translocation between chromosomes 9 and 22. The resultant BCR/ABL fusion protein displays constitutive tyrosine kinase activity, leading to the induction of aberrant proliferation and neoplastic transformation. The bone marrow (BM) microenv...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.19633
更新日期：2017-10-23 00:00:00
abstract：:Background: We previously demonstrated that Proline rich 11 (PRR11) gene is associated with the development and progression of tongue squamous cell carcinoma (TSCC), but the underlying mechanism is unknown. This study aimed to investigate the molecular mechanism underlying oncogenic potential of PRR11 in TSCC cells. M...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.29265
更新日期：2019-06-02 00:00:00
abstract：:Cancer-associated fibroblasts (CAFs), which are an important component of the tumor microenvironment, have been identified in the blood circulation of patients with cancer metastasis, and metastatic cancer cells can recruit circulating CAFs. However, primary carcinoma sites usually regulate the behavior of metastatic ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.27590
更新日期：2019-07-23 00:00:00
abstract：:Background: The effect of adjuvant chemotherapy on stage II colon cancer remains constantly controversial. Recently, however, several studies have reported the definite survival benefit of adjuvant chemotherapy (ACT) in T4 disease (stage IIB and IIC) among stage II colon cancer patients. The following study investigat...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.28917
更新日期：2019-01-29 00:00:00
abstract：:Background: In the various cancer, mast cells (MCs) infiltration is correlated with a worse prognosis. There is an increasing evidence that MCs and their mediators are participated in remodeling of the tumor microenvironment and facilitate tumor growth, epithelial-to-mesenchymal transition (EMT) and metastasis. Method...
journal_title：Journal of Cancer
pub_type： 杂志文章,收录出版
doi：10.7150/jca.29953
更新日期：2019-06-09 00:00:00
abstract：OBJECTIVE:The expression of DACH1 was frequently lost in human breast cancer, which significantly correlated with poor prognosis. Herein, we aim to investigate its underlying mechanisms. METHODS:The expression of miR-217 was detected by Taqman PCR. The mRNA and protein level of DACH1 were investigated by real time PCR...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.10822
更新日期：2015-01-01 00:00:00
abstract：:Purpose: The aberrant of fibroblast growth factors and their receptors (FGF/FGFR) is an emerging target in the treatment of solid tumors. This study aimed to explore the landscape of FGF/FGFR alterations in a large cohort of cancer patients. Material and Methods: The formalin-fixed paraffin-embedded specimens of cance...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.49269
更新日期：2020-09-23 00:00:00
abstract：:Objective: To enquire into the clinical significance and potential molecular mechanism of microRNA (miRNA)-196b-5p in hepatocellular carcinoma (HCC). Methods: Quantitative reverse transcription and polymerase chain reaction (qRT-PCR) were utilized to examine miR-196b-5p expression level in 67 HCC paraffin embedded tis...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.29293
更新日期：2019-08-29 00:00:00
abstract：PURPOSE:To investigate prognostic impact of chemoradiotherapy-induced hemoglobin (Hb) decrease on treatment outcomes of endemic nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS:Eight hundred and fifteen non-metastatic NPC, receiving neoadjuvant chemotherapy followed by radiotherapy (NACT+RT group) or concomitant ...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.11403
更新日期：2015-04-02 00:00:00
abstract：:Long non-coding RNAs (lncRNAs) have been considered as biomarkers for the carcinogenesis and development of various cancers. However, the prognostic significance of lncRNAs in renal cell carcinoma (RCC) remains unclear. This study aimed to determine the predictive ability of lncRNAs in clear cell RCC (ccRCC). Among th...
journal_title：Journal of Cancer
pub_type： 杂志文章
doi：10.7150/jca.40421
更新日期：2020-04-27 00:00:00
