Abstract:
:Background: Anti-EGFR therapies have been recommended for advanced colorectal cancer (CRC) with wild-type RAS and PIK3CA mutation. However, PIK3CA mutations are a poor prognostic marker and a negative predictor of response to anti-EGFR therapies in RAS wild-type CRC. Therefore, new and advanced treatment strategies are needed for personalized medical treatment of patients with wild-type RAS and PIK3CA mutation. Methods: Patient-derived tumor cells were collected from the ascites of a refractory colon cancer patient with wild-type RAS and PIK3CA mutation. We performed a cell viability assay for cetuximab, AZD5363 (AKT inhibitor), and everolimus (mTOR inhibitor) using PDCs. We also evaluated combinations of cetuximab plus AZD5363 or everolimus in a cell viability assay. Results: Based on cellular proliferation by MTT assay, tumor cells were significantly inhibited by 1uM cetuximab (control vs. cetuximab, mean growth = 100.0% vs 58.07%, p = 0.0103), 1uM AZD5363 (control vs. AZD5363, mean growth = 100.0% vs 58.22%, p = 0.0123), and 1uM everolimus (control vs. everolimus, mean growth = 100.0% vs 52.17%, p = 0.0011). Tumor cell growth was more profoundly reduced by combinations of cetuximab plus AZD5363 (control vs. cetuximab plus AZD5363, mean growth = 100.0% vs 25.00%, p < 0.0001) or everolimus (control vs. cetuximab+everolimus, mean growth = 100.0% vs 28.24%, p < 0.0001). Conclusions: Taken together, these results indicate that RAS wild-type and PIK3CA mutant PDCs originating from CRC are considerably inhibited by treatment with cetuximab plus AZD5363 or everolimus, with downregulation of the AKT and ERK pathways. These combinations may be considered as new options for advanced CRC patients with wild-type RAS and PIK3CA mutation in the context of clinical trials.
journal_name
J Cancerjournal_title
Journal of Cancerauthors
Kim JS,Kim JE,Kim K,Lee J,Park JO,Lim HY,Park YS,Kang WK,Kim STdoi
10.7150/jca.19458subject
Has Abstractpub_date
2017-08-22 00:00:00pages
2713-2719issue
14issn
1837-9664pii
jcav08p2713journal_volume
8pub_type
杂志文章abstract::Ever since its discovery (1924) the Warburg effect (aerobic glycolysis) remains an unresolved puzzle: why the aggressive cancer cells "prefer" to use the energetically highly inefficient method of burning the glucose at the cellular level? While in the course of the last 90 years several hypotheses have been suggested...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
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更新日期:2016-04-26 00:00:00
abstract:BACKGROUND AND PURPOSE:There is increasing evidence that the presence of an inflammation-based prognostic score (modified Glasgow prognostic score, mGPS) is associated with survival in patients with advanced cancer. This study aimed to assess whether the mGPS has prognostic value in patients with thoracic esophageal sq...
journal_title:Journal of Cancer
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更新日期:2014-09-06 00:00:00
abstract::Background and aims. Cyclin-dependent kinase inhibitor 3 (CDKN3) has been found playing a varying role in carcinogenesis, but its biological function in esophageal squamous cell carcinoma (ESCC) is unclear. The aim of this study was to demonstrate the role of CDKN3 in ESCC. Materials and Methods: Real-time PCR and Wes...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.27053
更新日期:2019-04-21 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a major malignancy of cancer-related mortality worldwide. Metastasis-associated in colon cancer-1 (MACC1) was suggested as a marker for vascular invasive HCC. This study investigated the MACC1 single-nucleotide polymorphisms (SNPs) to evaluate HCC susceptibility and clinicopathologica...
journal_title:Journal of Cancer
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更新日期:2020-01-14 00:00:00
abstract::Background: Early gastric carcinoma (EGC) with pure signet ring cell carcinoma (pSRCC) has been reported to have favourable prognosis and low risk of lymph node metastasis (LNM). However, risk factors of LNM and clinicopathological features for early gastric mixed signet ring cell carcinoma (mSRCC) remain poorly inves...
journal_title:Journal of Cancer
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更新日期:2019-01-29 00:00:00
abstract::Background and objective: Long-term aspirin use for the primary prevention of cancer remains controversial, and variations in the effect of aspirin use on cancer outcomes by aspirin dose, follow-up duration, or study population have never been systematically evaluated. The objective of this study was to evaluate the e...
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abstract::Background: This study aimed to clarify the impact of node involvement (affected to resected nodes) in optimally cytoreduced (residual tumour ≤1cm) stage IIIC/IV ovarian cancer. Methods: 108 consecutive patients with primary stage IIIC/IV ovarian cancer underwent stage-related surgery and got adjuvant platinum-based c...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.15644
更新日期:2016-11-09 00:00:00
abstract::Long non-coding RNAs (LncRNAs) have been reported to be involved in tumorigenesis and tumor progression. Single nucleotide polymorphisms (SNPs) in the lncRNAs also play a vital role in carcinogenesis. The aim of this study was to assess the relationships between the four selected tagSNPs (rs944289, rs3787016, rs145631...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.18055
更新日期:2017-04-09 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.11403
更新日期:2015-04-02 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2019-01-01 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2020-07-31 00:00:00
abstract::Non epithelial ovarian tumors represent a small group of rare gynecological cancers but they have a high incidence in young childbearing women. The recent years fertility preservation surgeries have become a common practice in specific ovarian malignancies when the patients wish to maintain their fertility. Specific t...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.26674
更新日期:2018-11-24 00:00:00
abstract::Tyrosine phosphorylation is an important post-translation modification of proteins that is controlled by tyrosine kinases and phosphatases. Disruption of the balance between the activity of tyrosine kinases and phosphatases may result in diseases. Receptor type protein tyrosine phosphatase epsilon (PTPRE) is closely r...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.27633
更新日期:2019-01-01 00:00:00
abstract::Triple negative breast cancer (TNBC) is characterized by distinctive biological features that confer an aggressive clinical behavior. In TNBC patients, the absence of well-defined driver pathways such as hormonal receptor expression or hyperactivation of the human epidermal growth factor receptor 2 (HER2) significantl...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.35109
更新日期:2019-10-12 00:00:00
abstract::Various factors modulate the risk of hepatoblastoma. In this study, we aimed to investigate whether single nucleotide polymorphisms (SNPs) in the YTHDF1 gene could predispose to hepatoblastoma. We used TaqMan assay to genotype two YTHDF1 SNPs (rs6011668 C>T and rs6090311 A>G) in a Chinese population composed of 313 su...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.46120
更新日期:2020-06-28 00:00:00
abstract::Background: Most hepatocellular carcinoma (HCC) patients have undergone a progression from chronic hepatitis, then liver cirrhosis (LC), and finally to carcinoma. The objective of this study was to elucidate risk factors to predict HCC development for cirrhosis patients. Methods: Multiple methylated specific PCR (MSP)...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.24024
更新日期:2018-06-04 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma (PPARγ), a member of the nuclear receptor superfamily, participates in multiple physiological and pathological processes. Extensive studies have revealed the relationship between PPARγ and various tumors. However, the expression and function of PPARγ in bladder cancer s...
journal_title:Journal of Cancer
pub_type: 杂志文章,评审
doi:10.7150/jca.42663
更新日期:2020-04-06 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.5419
更新日期:2013-01-01 00:00:00
abstract::Purpose: To design an alternative workflow for cancer-risk assessment to predict distant metastasis (DM) of nasopharyngeal carcinoma (NPC). Methods: We enrolled 234 patients with non-disseminated NPC and a family history of cancer who underwent intensity-modulated radiotherapy and concurrent chemo-radiotherapy with/wi...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.19611
更新日期:2017-07-20 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.32281
更新日期:2019-06-09 00:00:00
abstract::Esophageal cancer (EC) is a unique and heterogeneous disease diagnosed mostly at advanced stages. Altered glycans presented on cell surfaces are involved in the occurrence and development of malignancy. However, the effects of glycans on EC progression are largely unexplored. Here, a lectin array was utilized to detec...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.43806
更新日期:2020-06-01 00:00:00
abstract::Philadelphia chromosome-positive (Ph+) leukemia is characterized by reciprocal translocation between chromosomes 9 and 22. The resultant BCR/ABL fusion protein displays constitutive tyrosine kinase activity, leading to the induction of aberrant proliferation and neoplastic transformation. The bone marrow (BM) microenv...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.19633
更新日期:2017-10-23 00:00:00
abstract::Purpose Neuroblastoma is an embryonic solid tumor derived from the progenitors of the sympathetic nervous system. More than half of the patients developed metastatic disease at the time of initial diagnosis and had poor outcome with current therapeutic approaches. In recent years, some obligate and facultative anaerob...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.18776
更新日期:2017-06-03 00:00:00
abstract::Thyroid transcription factor-1 (TTF-1) is overexpressed in up to 95% of primary lung adenocarcinoma while negative for almost all squamous cell carcinomas. TTF-1 expression has been investigated as a prognostic factor in non-small-cell lung cancer (NSCLC) with conflicting results. We conducted this meta-analysis to ga...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.26830
更新日期:2018-10-20 00:00:00
abstract::Despite advancement in the treatment of diffuse large B-cell lymphoma (DLBCL), many patients tend to relapse or become refractory after initial therapy. Therefore, it is essential to identify novel therapeutic targets and drugs, understand the molecular pathogenesis mechanism of DLBCL, and find ways to prevent and tre...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.48310
更新日期:2021-01-01 00:00:00
abstract:INTRODUCTION:Hürthle cell carcinoma (HCC) is a rare tumor that tends to metastasize to the lymph nodes. Some studies have correlated size of Hürthle cell tumors with the risk of malignancy. Whether the size of HCC correlates with the risk of lymph node (LN) metastases, to our knowledge has not been addressed. METHODS:...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.1.23
更新日期:2010-06-02 00:00:00
abstract:AIM:The aim of our study was to compare clinical outcomes between elderly patients aged ≥75 years (elderly group, n=66) with intermediate hepatocellular carcinoma (HCC) undergoing transcatheter arterial chemoembolization (TACE) and younger patients aged <75 years (control group, n=84) with intermediate HCC undergoing T...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.9413
更新日期:2014-07-17 00:00:00
abstract::Background: Growing data suggest that DNA damage repair and detoxification pathways play crucial roles in radiation-induced toxicities. To determine whether common functional single-nucleotide polymorphisms (SNPs) in candidate genes from these pathways can be used as predictors of radiation pneumonitis (RP), we conduc...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.20643
更新日期:2018-02-01 00:00:00
abstract:PURPOSE:Chemotherapy regimens containing anthracyclines and taxanes represent the landmark of neoadjuvant systemic therapy of breast cancer. In advanced breast cancer patients liposomal anthracyclines (LA) have shown similar efficacy and less cardiac toxicity when compared to conventional anthracyclines. We performed t...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.9132
更新日期:2014-04-25 00:00:00
abstract::Butyrate, a product of dietary fiber and a histone deacetylase inhibitor, induces apoptosis of colorectal cancer cells; this effect of butyrate is in part mediated by its ability to hyperactivate Wnt signaling, and may in part explain the preventive action of dietary fiber against colorectal cancer. However, the mecha...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.37864
更新日期:2020-01-01 00:00:00