Abstract:
:Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide. However, science has not yet been able to substantially improve the prognosis of lung cancer patients. Accumulating evidence suggests that microRNAs (miRNAs) are key players in the regulation of tumor development and metastasis. Expression of six miRNAs previously shown to play roles in tumor development (miR-146b-5p, miR-128b, miR-21, miR-221, miR-34a, and Let-7a) in other tumor types was examined using real-time RT-PCR in 78 specimens of NSCLC. The results revealed that patients with low expression of miR-146b-5p had significant shorter median and mean survival time than those with high miR-146b-5p expression (33.00 and 30.44 months versus 42.0 and 36.90 months, respectively; log-rank test P=0.048), thus low miR-146b-5p expression level was associated with poor prognosis in NSCLC patients. Univariate Cox hazard regression analysis demonstrated that miR-146b-5p expression levels tended to be a significant prognostic indicator of NSCLC (adjusted hazard ratio=0.482, 95% CI: 1.409- 29.593, P=0.016). Multivariate Cox proportional hazard regression analysis showed that miR-146b-5p expression levels were an independent prognostic factor for NSCLC patients (hazard ratio=0.259, 95% CI: 0.083-0.809, P=0.020). Furthermore, the effects of miR-146b-5p and miR-146b-3p on NSCLC cell growth and invasion in vitro were investigated. Our findings demonstrate that ectopic expression of miR-146b-5p suppressed cell proliferation, clonogenicity, migration/ invasion and also induced G1 arrest in vitro, but did not induce cell apoptosis; whereas enforced expression of miR-146b-3p did not have a significant effect on cell growth and metastasis. Further experiments indicated that miR-146b-5p could reduce mRNA levels of MMP16 and TRAF6 in vitro and was negatively related to the expression of TRAF6 in human NSCLC tissues. In a mouse model, Ago-miR-146b-5p could significantly inhibit the growth of lung cancer xenografts in nude mice. In conclusion, our findings demonstrate that miR-146b-5p functions as a suppressor miRNA and prognosis predictor in NSCLC.
journal_name
J Cancerjournal_title
Journal of Cancerauthors
Li Y,Zhang H,Dong Y,Fan Y,Li Y,Zhao C,Wang C,Liu J,Li X,Dong M,Liu H,Chen Jdoi
10.7150/jca.16961subject
Has Abstractpub_date
2017-06-30 00:00:00pages
1704-1716issue
9issn
1837-9664pii
jcav08p1704journal_volume
8pub_type
杂志文章abstract::In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine ...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.4163
更新日期:2012-01-01 00:00:00
abstract::There is limited literature documenting granulocytic sarcoma of the colon. We report a case of a 28 year-old female with a colonic granulocystic sarcoma of the colon as a complication of AML, as it is an important consideration with surgical management of typhilitis. ...
journal_title:Journal of Cancer
pub_type:
doi:10.7150/jca.1.98
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abstract::Background: To support cancer therapy, development of low cost library preparation techniques for targeted next generation sequencing (NGS) is needed. In this study we designed and tested a PCR-based library preparation panel with limited target area for sequencing the top 12 somatic mutation hot spots in colorectal c...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.16037
更新日期:2017-01-12 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.9794
更新日期:2014-10-28 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.2.36
更新日期:2011-01-08 00:00:00
abstract::Alternative splicing plays critical roles in many disease processes and splicing dysregulation is a hallmark of cancer. The different splicing isoforms may have significantly different effects on the malignant progression of cancer. Checkpoint kinase 1 (CHK1) is a serine/threonine kinase and regulates DNA damage respo...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.39443
更新日期:2020-01-17 00:00:00
abstract:BACKGROUND:MALAT-1 is significantly overexpressed in various cancers, suggesting that it might be a potential biomarker of cancer. METHODS:A meta-analysis was performed using microarray data obtained via the Affymetrix Human Genome U133 Plus 2.0 platform found in the Gene Expression Omnibus (GEO) database and data obt...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.14663
更新日期:2016-05-20 00:00:00
abstract::Objective: Liposarcoma is a mesenchymal malignant tumor characterized by adipocyte differentiation which is divided into four subtypes with different prognosis. Accurate histopathological diagnosis is essential for precise treatment. Perilipins, including PLIN1, PLIN2, PLIN3, PLIN4, PLIN5, is a family of lipid droplet...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.41736
更新日期:2020-04-07 00:00:00
abstract::Colorectal cancer (CRC) is an important health issue worldwide, accounting for the third place of cancer incidence. Chronic inflammation, as seen in Crohn's disease and ulcerative colitis, is the most important risk factor for developing CRC, as it favours neoplastic transformation by enhancing epithelial cell turnove...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.13123
更新日期:2016-07-25 00:00:00
abstract::Metabolic syndrome has been previously identified as a risk factor for breast cancer and is increasingly a public health concern. This study aims to investigate the prevalence of metabolic syndrome and its components among primary breast cancer and control population. The clinical data of metabolic syndrome and its co...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.22968
更新日期:2018-01-01 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.16331
更新日期:2016-08-12 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.46902
更新日期:2020-08-25 00:00:00
abstract::Background: Radiotherapy is a primary treatment strategy for patients with unresectable hepatocellular carcinoma (HCC); however, the prognostic factors among HCC patients who have received radiotherapy but not undergone surgery have not been systematically studied. Thus, the prognostic factors were investigated in thi...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.30365
更新日期:2019-07-25 00:00:00
abstract::Purpose: The early BCR-ABL1 reduction had the prognostic impact of the chronic-phase chronic myeloid leukemia (CML-CP) patients. This study was to find a more precise early prognosis index at 3 months in the patients with newly diagnosed CML-CP, especially for the patients with BCR-ABL1IS >10%. Methods: We retrospecti...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.38752
更新日期:2020-02-03 00:00:00
abstract::Objective: Both previous and recent literature showed long non-coding RNAs (lncRNAs) were crucial participants in multiple myeloma (MM) evolution. However, the dynamic regulation and mechanism of lncRNAs in MM progression was not fully understood. This study will explore the expression and effects of prostate cancer-a...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.35098
更新日期:2019-10-20 00:00:00
abstract::Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential enzyme in the base excision repair pathway. Epidemiological studies have suggested associations between APE1 rs1760944 polymorphism and cancer risk. This study was aimed to evaluate the relationship between APE1 rs1760944 polymorphism and cancer risk. We sear...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.8085
更新日期:2014-03-13 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.34471
更新日期:2019-09-07 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.41135
更新日期:2020-03-13 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.35097
更新日期:2019-10-15 00:00:00
abstract::Endometrial carcinoma (EC) is the most common malignant tumors in female derived from the endometrial epithelium. Several previous studies have described estrogen receptors (ER), progesterone Receptor (PR) and phosphatase and tensin homolog (PTEN) are associated with clinicopathological factors and prognosis in EC pat...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.33720
更新日期:2020-01-13 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2017-08-22 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2018-04-30 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2016-01-01 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.2.329
更新日期:2011-01-01 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.22318
更新日期:2018-01-01 00:00:00
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journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2019-07-10 00:00:00
abstract::Background: Noninvasive stool-based DNA methylation testing emerges as a new approach for detecting colorectal cancer (CRC). However, its feasibility for early detection of CRC and precancerous lesions in the Chinese population remains inconclusive. Methods: In this study, we establish a possibilities screening method...
journal_title:Journal of Cancer
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
abstract::Esophageal cancer (EC) is a unique and heterogeneous disease diagnosed mostly at advanced stages. Altered glycans presented on cell surfaces are involved in the occurrence and development of malignancy. However, the effects of glycans on EC progression are largely unexplored. Here, a lectin array was utilized to detec...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.43806
更新日期:2020-06-01 00:00:00
abstract::We found that microRNA-320a (miR-320a) was an attractive prognostic biomarker in breast cancer (BC) previously, whereas its regulatory mechanism in BC was not well understood. Our aim was to identify miR-320a target gene, examine the clinical relationship between miR-320a and its target, and further explore the functi...
journal_title:Journal of Cancer
pub_type: 杂志文章
doi:10.7150/jca.15737
更新日期:2016-11-25 00:00:00
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journal_title:Journal of Cancer
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更新日期:2020-01-01 00:00:00