Long non-coding RNA LOC554202 promotes acquired gefitinib resistance in non-small cell lung cancer through upregulating miR-31 expression.

Abstract:

:Non-small-cell lung cancer (NSCLC) patients with epidermal growth factor receptor (EGFR) mutation inevitably have a relapse due to the occurrence of acquired resistance, resulting in treatment failure. However, little is known about the mechanisms of acquired resistance of NSCLC patients. Here, we elucidated the expression pattern of LOC554202 and miR-31, and their biological functions and mechanisms in NSCLC with acquired EGFR TKI resistance to gefitinib. In the present study, we observed that LOC554202 and miR-31 promoted proliferation and clonogenic growth of gefitinib-resistant NSCLC cells in vitro. LOC554202 upregulated miR-31 expression and they both reduced sensitivity of NSCLC cells to gefitinib. In a xenograft mice model, we found that knockdown of miR-31 significantly repressed gefitinib-resistant NSCLC cells growth in vivo. Furthermore, both LOC554202 and miR-31 levels were significantly increased in NSCLC patients acquiring resistance to gefitinib, and the expression of LOC554202 was positively correlated with the expression of miR-31. By luciferase reporter assays, we identified RAS P21 Protein Activator 1 (RASA1) and Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor (FIH-1) as direct targets of miR-31 in NSCLC cells. Mechanistically, miR-31 directly repressed RASA1 and FIH-1 expression, and thus, at least partially activated the RAF-MEK-ERK and PI3K-AKT signaling pathways in NSCLC with acquired resistance to gefitinib. In conclusion, these data will help us develop potential therapeutic targets for the diagnosis and treatment of acquired EGFR TKI resistance in EGFR-mutant NSCLC.

journal_name

J Cancer

journal_title

Journal of Cancer

authors

He J,Jin S,Zhang W,Wu D,Li J,Xu J,Gao W

doi

10.7150/jca.35097

subject

Has Abstract

pub_date

2019-10-15 00:00:00

pages

6003-6013

issue

24

issn

1837-9664

pii

jcav10p6003

journal_volume

10

pub_type

杂志文章
  • Hypoxia Inducible Factor-1alpha (HIF-1A) plays different roles in Gallbladder Cancer and Normal Gallbladder Tissues.

    abstract::Purpose: Hypoxia-inducible factor-1alpha (HIF-1A) is a transcription factor that plays an "angiogenic switch" role especially under hypoxia microenvironment in solid tumor. However, the functions and clinical significance of HIF-1A in gallbladder cancer (GBC) are still controversial, and it has not been studied in nor...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.46749

    authors: Wu Y,Meng D,You Y,Sun R,Fu M,Yan Q,Zhang S,Fang Z,Bao J,Li Y

    更新日期:2021-01-01 00:00:00

  • Diagnostic and prognostic biomarkers of Human Leukocyte Antigen complex for hepatitis B virus-related hepatocellular carcinoma.

    abstract::Background: Hepatitis B virus infection had been identified its relationship with liver diseases, including liver tumors. We aimed to explore diagnostic and prognostic values between the Human Leukocyte Antigen (HLA) complex and hepatocellular carcinoma (HCC). Methods: We used the GSE14520 dataset to explore diagnosti...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.29655

    authors: Wang XK,Liao XW,Yang CK,Yu TD,Liu ZQ,Gong YZ,Huang KT,Zeng XM,Han CY,Zhu GZ,Qin W,Peng T

    更新日期:2019-08-28 00:00:00

  • A quick, cost-free method of purification of DNA fragments from agarose gel.

    abstract::In this short communication we report a quick, cost-free method of purification of DNA fragments from agarose gel. Unlike those procedures that involve commercial kits, this method uses glass wool or absorbent cotton to filter agarose gel during a quick spinning-down of DNA, thus significantly simplifying the routine ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.4163

    authors: Sun Y,Sriramajayam K,Luo D,Liao DJ

    更新日期:2012-01-01 00:00:00

  • Diagnostic accuracy of frozen section analysis of borderline ovarian tumors: a meta-analysis with emphasis on misdiagnosis factors.

    abstract::Objective: First, to evaluate the sensitivity and positive predictive value (PPV) of intra-operative frozen section (FS) diagnosis in borderline ovarian tumors (BOTs), and to explore the factors affecting the diagnostic accuracy. Second, to assess the clinical outcomes of misdiagnosed BOT patients. Methods: We perform...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.25883

    authors: Huang Z,Li L,Li C,Ngaujah S,Yao S,Chu R,Xie L,Yang X,Zhang X,Liu P,Jiang J,Zhang Y,Cui B,Song K,Kong B

    更新日期:2018-07-16 00:00:00

  • Significant prognostic impact of chemoradiotherapy-induced hemoglobin decrease on treatment outcomes of nasopharyngeal carcinoma.

    abstract:PURPOSE:To investigate prognostic impact of chemoradiotherapy-induced hemoglobin (Hb) decrease on treatment outcomes of endemic nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS:Eight hundred and fifteen non-metastatic NPC, receiving neoadjuvant chemotherapy followed by radiotherapy (NACT+RT group) or concomitant ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.11403

    authors: Liang XX,Li Q,Su Z,Lan XW,Ouyang PY,Mao YP,Shi DB,Deng WG,Cheng ZB,Wang SY,Xie FY

    更新日期:2015-04-02 00:00:00

  • Novel biomarkers and prediction model for the pathological complete response to neoadjuvant treatment of triple-negative breast cancer.

    abstract::Objective: To develop and validate a prediction model for the pathological complete response (pCR) to neoadjuvant chemotherapy (NCT) of triple-negative breast cancer (TNBC). Methods: We systematically searched Gene Expression Omnibus, ArrayExpress, and PubMed for the gene expression profiles of operable TNBC accessibl...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.52439

    authors: Han Y,Wang J,Xu B

    更新日期:2021-01-01 00:00:00

  • A Predictive Nomogram for Early Death of Metastatic Gastric Cancer: A Retrospective Study in the SEER Database and China.

    abstract::Background: To identify associated risk factors and develop a predictive nomogram for the early death of metastatic gastric cancer patients. Methods: A total of 4575 patients in the SEER cohort and 220 patients in the Chinese cohort diagnosed with metastatic gastric cancer in our Cancer Center were obtained. Univariat...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.46563

    authors: Zhu Y,Fang X,Wang L,Zhang T,Yu D

    更新日期:2020-07-14 00:00:00

  • Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor.

    abstract::MPHOSPH1, which is one of the kinesin superfamily proteins, has been reported to play an essential role in the carcinogenesis and progression of several kinds of cancers. MPHOSPH1 has also been suggested to be involved in STAT3 phosphorylation in hepatocellular carcinoma. However, the biological behavior of MPHOSPH1 i...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.25279

    authors: Abe T,Kohashi K,Takemoto J,Kinoshita F,Eto M,Oda Y

    更新日期:2018-10-31 00:00:00

  • Disruption of learning processes by chemotherapeutic agents in childhood survivors of acute lymphoblastic leukemia and preclinical models.

    abstract:OBJECTIVE:With the survival rate of acute lymphoblastic leukemia (ALL) surpassing 90 percent within this decade, new research is emerging in the field of late effects. A review of the research investigating the relationship of treatment regimens for ALL to specific late effect deficits, underlying mechanisms, and possi...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.2.292

    authors: Bisen-Hersh EB,Hineline PN,Walker EA

    更新日期:2011-01-01 00:00:00

  • Gustave Roussy Immune Score (GRIm-Score) is a prognostic marker in patients with resectable esophageal squamous cell carcinoma.

    abstract::Background: The Gustave Roussy Immune Score (GRIm-Score) was initially reported to select patients for immunotherapy. Therefore, the purpose of the current retrospective study was to determine whether GRIm-Score, a novel nutritional and inflammatory-based prognostic score, is a useful prognostic marker in patients wit...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.37898

    authors: Feng JF,Wang L,Yang X,Chen S

    更新日期:2020-01-01 00:00:00

  • Total PC Activity Is Increased in Uterine Lavage of Post-Menopausal Endometrial but Not Ovarian Cancer Patients.

    abstract::Endometrial and ovarian cancers are two most common cancers affecting women in their post-menopausal years. To date, there are no simple biochemical tests to detect these cancers at early stages. Our previous study has demonstrated that the activity of total proprotein convertases (PCs) is significantly increased in u...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.16331

    authors: Heng S,Stephens AN,Jobling TW,Nie G

    更新日期:2016-08-12 00:00:00

  • Blood-based Genomic Profiling of Circulating Tumor DNA from Patients with Advanced Pancreatic Cancer and its Value to Guide Clinical Treatment.

    abstract::Objective: Pancreatic cancer (PC) is a malignant tumor with limited therapeutic choices and extremely poor prognosis. Personalized therapy based on gene alternations is a promising choice. Considering tumor heterogeneity, the practice of ctDNA analysis has drawn the attention. Here, we try to assess the applicability ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.43087

    authors: Li H,Di Y,Li J,Jiang Y,He H,Yao L,Gu J,Lu J,Song J,Chen S,Cai S,Jin C,Yuan Z,Fu D

    更新日期:2020-04-27 00:00:00

  • Lenvatinib in Advanced Radioiodine-Refractory Thyroid Cancer - A Retrospective Analysis of the Swiss Lenvatinib Named Patient Program.

    abstract:: Purpose: Differentiated thyroid cancer (DTC) accounts for approximately 95% of thyroid carcinomas. In the metastatic RAI-refractory disease, chemotherapy has very limited efficacy and is associated with substantial toxicity. With increasing knowledge of the molecular patho...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.22318

    authors: Balmelli C,Railic N,Siano M,Feuerlein K,Cathomas R,Cristina V,Güthner C,Zimmermann S,Weidner S,Pless M,Stenner F,Rothschild SI

    更新日期:2018-01-01 00:00:00

  • Distribution and Clinical Utility of the 21-gene Recurrence Score in Pure Mucinous Breast Cancer Patients: a case-control study.

    abstract::The 21-gene recurrence score (RS) is increasingly being used for patients with early stage, hormone receptor-positive, Her-2-negative breast cancer. However, these results are largely from populations with infiltrating ductal carcinoma (IDC). The clinical value of RS testing in mucinous carcinoma has not been well inv...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.27291

    authors: Wang W,Chen X,Lin L,Fei X,Garfield DH,Hong J,Gao W,Zhu S,Wu J,Huang O,He J,Li Y,Zhu L,Chen W,Shen K

    更新日期:2018-08-06 00:00:00

  • Association of Body Mass Index Changes during Neoadjuvant Chemotherapy with Pathologic Complete Response and Clinical Outcomes in Patients with Locally Advanced Breast Cancer.

    abstract::The purpose of this study was to determine the association between body mass index (BMI) measurements (baseline BMI and changes in BMI during neoadjuvant systemic treatment [NST]) and clinical efficacy (pathologic complete response [pCR] rate and survival outcomes) in locally advanced breast cancer (LABC). We hypothes...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.10580

    authors: Kogawa T,Fouad TM,Wei C,Masuda H,Kai K,Fujii T,El-Zein R,Chavez-MacGregor M,Litton JK,Brewster A,Alvarez RH,Hortobagyi GN,Valero V,Theriault R,Ueno NT

    更新日期:2015-01-22 00:00:00

  • Circulating Lymphocytes, PD-L1 Expression on Tumor-infiltrating Lymphocytes, and Survival of Colorectal Cancer Patients with Different Mismatch Repair Gene Status.

    abstract::Clinical outcomes of checkpoint blockade immunotherapy on colorectal cancer (CRC) are influenced by mismatch repair (MMR) gene status, which is associated with distinct tumor immune infiltrates and systemic inflammatory response status. However, the prognostic value of PD-L1 expression and the systemic inflammatory re...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.25187

    authors: Kong P,Wang J,Song Z,Liu S,He W,Jiang C,Xie Q,Yang L,Xia X,Xia L

    更新日期:2019-04-05 00:00:00

  • A Majority of Low (1-10%) ER Positive Breast Cancers Behave Like Hormone Receptor Negative Tumors.

    abstract:BACKGROUND:The 2010 guidelines by ASCO-CAP have mandated that breast cancer specimens with ≥1% positively staining cells by immunohistochemistry should be considered Estrogen Receptor (ER) positive. This has led to a subclass of low-ER positive (1-10%) breast cancers. We have examined the biology and clinical behavior ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.7668

    authors: Prabhu JS,Korlimarla A,Desai K,Alexander A,Raghavan R,Anupama C,Dendukuri N,Manjunath S,Correa M,Raman N,Kalamdani A,Prasad M,Gopinath KS,Srinath BS,Sridhar TS

    更新日期:2014-01-23 00:00:00

  • Improved Prognostic Prediction of Glioblastoma using a PAS Detected from Single-cell RNA-seq.

    abstract::Glioblastoma (GBM) is a common malignant brain tumor of the central nervous system with a poor prognosis. In order to identify the prognostic signatures of GBM, we screened differentially expressed genes (DEGs) that were based on a single-cell RNA sequencing (scRNA-seq) dataset. These genes characteristically represen...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.44034

    authors: Liu H,Yang Q,Xiong Y,Xiong Z,Li X

    更新日期:2020-04-06 00:00:00

  • Progression-free Survival Decreases with Each Subsequent Therapy in Patients Presenting for Phase I Clinical Trials.

    abstract:BACKGROUND:There is often a finite progression-free interval of time between one systemic therapy and the next when treating patients with advanced cancer. While it appears that progression-free survival (PFS) between systemic therapies tends to get shorter for a number of factors, there has not been a formal evaluatio...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.3.7

    authors: Bailey CH,Jameson G,Sima C,Fleck S,White E,Von Hoff DD,Weiss GJ

    更新日期:2012-01-01 00:00:00

  • Comparison of Clinicopathological Features and Prognosis in Triple-Negative and Non-Triple Negative Breast Cancer.

    abstract:PURPOSE:Triple-negative breast cancer (TNBC) has attracted more attention both clinically and experimentally because of its high-risk biological characteristics and lacking of effective treatment method. The purpose of this retrospective study was to find out the incidence of triple-negative breast cancer (TNBC) in all...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.10944

    authors: Qiu J,Xue X,Hu C,Xu H,Kou D,Li R,Li M

    更新日期:2016-01-01 00:00:00

  • Association between the KRAS Gene Polymorphisms and Papillary Thyroid Carcinoma in a Chinese Han Population.

    abstract::Several studies have reported the association between MAPK signaling pathway gene polymorphisms and papillary thyroid carcinoma (PTC). KRAS gene, an oncogene from the mammalian RAS gene family plays an important role in the MAPK pathway. This study aimed to identify the potential association of KRAS gene polymorphisms...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.16507

    authors: Ning L,Rao W,Yu Y,Liu X,Pan Y,Ma Y,Liu R,Zhang S,Sun H,Yu Q

    更新日期:2016-12-09 00:00:00

  • Efficacy of Cabazitaxel Treatment in Metastatic Castration Resistant Prostate Cancer in Second and Later Lines. An Experience from Two German Centers.

    abstract::Purpose: Several new treatment options for patients with metastatic castration resistant prostate cancer (mCRPC) have been approved within the last years - among them cabazitaxel (CAB), abiraterone acetate, enzalutamide, and radium-223. The aim of this study was to assess factors predictive for efficacy of CAB. Method...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.17644

    authors: Zschäbitz S,Vallet S,Hadaschik B,Debatin D,Fuxius S,Karcher A,Pahernik S,Spath C,Duensing S,Jäger D,Hohenfellner M,Grüllich C

    更新日期:2017-02-11 00:00:00

  • Anti-inflammatory and Antitumor Activity of a Triple Therapy for a Colitis-Related Colorectal Cancer.

    abstract::Colorectal cancer (CRC) is an important health issue worldwide, accounting for the third place of cancer incidence. Chronic inflammation, as seen in Crohn's disease and ulcerative colitis, is the most important risk factor for developing CRC, as it favours neoplastic transformation by enhancing epithelial cell turnove...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.13123

    authors: Figueroa-González G,García-Castillo V,Coronel-Hernández J,López-Urrutia E,León-Cabrera S,Arias-Romero LE,Terrazas LI,Rodríguez-Sosa M,Campos-Parra AD,Zúñiga-Calzada E,Lopez-Camarillo C,Morales-González F,Jacobo-Herrera NJ,Pérez

    更新日期:2016-07-25 00:00:00

  • Integrin-mediated Signaling via Paxillin-GIT1-PIX Promotes Localized Rac Activation at the Leading Edge and Cell Migration.

    abstract::Rac activation is precisely regulated temporally and spatially by intracellular signaling pathways in migrating cells to guarantee the formation of specific cell protrusions-lamellipodia at the leading edge. Integrins-mediated adhesions also control the signaling pathway for localized Rac activation in the cells, but ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.32853

    authors: Li Y,Wu X,Liu Z,Lu K,Liu R,Guo X

    更新日期:2020-01-01 00:00:00

  • Pulmonary Tuberculosis is Associated with Elevated Risk of Lung cancer in Korea: The Nationwide Cohort Study.

    abstract::Objective: Although previous studies suggest that previous pulmonary tuberculosis was associated with increased risk of lung cancer. It remains controversial whether pulmonary tuberculosis is a risk factor for lung cancer. Our study was aimed to examine the association between pulmonary tuberculosis and lung cancer ri...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.37022

    authors: Oh CM,Roh YH,Lim D,Kong HJ,Cho H,Hwangbo B,Won YJ,Jung KW,Oh K

    更新日期:2020-01-20 00:00:00

  • Surgery plus chemotherapy improves survival of patients with extremity soft tissue leiomyosarcoma and metastasis at presentation.

    abstract::Purpose: Extremity soft tissue leiomyosarcoma (LMS) with metastasis is a rare disease with a poor prognosis. The purpose of our study was to define clinical features of extremity soft tissue LMS with metastasis as well as to identify multivariable predictors of survival. Methods: During 1973-2015, 239 patients with me...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.29874

    authors: Qian SJ,Wu JQ,Wang Z,Zhang B

    更新日期:2019-05-21 00:00:00

  • Impact of menopausal status and HER-2/neu protein on efficacy of EGFR-TKI in EGFR mutant patients with non-small cell lung cancer.

    abstract::Clinical studies have confirmed epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) used in lung cancer patients with EGFR mutations can obtain a better result, but still part of the patients with poor efficacy. EGFR mutation is highly related to female, nonsmoking and adenocarcinoma. Thus, we hy...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.25679

    authors: Yin ZJ,Tu HY,Fu M,Zhong WZ,An SJ,Yan HH,Chen HJ,Lin HR,Wu YL

    更新日期:2018-07-30 00:00:00

  • Landscape of FGF/FGFR Alterations in 12,372 Chinese Cancer Patients.

    abstract::Purpose: The aberrant of fibroblast growth factors and their receptors (FGF/FGFR) is an emerging target in the treatment of solid tumors. This study aimed to explore the landscape of FGF/FGFR alterations in a large cohort of cancer patients. Material and Methods: The formalin-fixed paraffin-embedded specimens of cance...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.49269

    authors: Zuo W,He Y,Li W,Wu H,Zhao Z,Zhang Y,Chen S,Yin Y

    更新日期:2020-09-23 00:00:00

  • High serum Androgen and Insulin concentrations increase the tendency of Endometrial Carcinoma.

    abstract::Purpose: The objective of the study was to evaluate the important role played by androgen and insulin in the development of endometrial carcinoma (EC), and their combined effect on EC risk. Methods: We enrolled 510 type I EC patients and 510 age-, time-, and nationality-matched subjects into this study. Metabolic and ...

    journal_title:Journal of Cancer

    pub_type: 杂志文章

    doi:10.7150/jca.46391

    authors: Teng F,Ma X,Yu X,Yan Y,Zhao J,Gao J,Gao C,Wang Y,Tian W,Xue F

    更新日期:2020-07-25 00:00:00

  • Effect of targeted agents on the endocrine response of breast cancer in the neoadjuvant setting: a systematic review.

    abstract::Inhibition of aberrantly activated pathways cross-talking with hormone receptor (HR) improves response to endocrine therapy in patients with HR-positive advanced breast cancer. We performed a Pubmed database systematic review to ascertain the existence of a better clinical response when combining endocrine therapy wit...

    journal_title:Journal of Cancer

    pub_type: 杂志文章,评审

    doi:10.7150/jca.11566

    authors: Grassadonia A,Caporale M,Tinari N,Zilli M,DeTursi M,Gamucci T,Vici P,Natoli C

    更新日期:2015-05-12 00:00:00