The IGF-I/IGFBP-3 system in gingival crevicular fluid and dependence on application of fixed force.

Abstract:

BACKGROUND:During application of orthodontic force on the tooth, various molecular parameters associated with tissue remodeling are changed. IGF-I is a regulatory protein produced during periodontal regeneration. IGF binding proteins-3 (IGFBP-3), a specific IGF-I binding protein, is the major regulatory factor of IGF-I activity. OBJECTIVES:We tested the hypothesis that changes in the IGF-I/ IGFBP-3 system occur during fixed force application to the tooth and that these changes are detectable in the gingival crevicular fluid (GCF). METHODS:IGFBP-3 and IGF-I secretion into gingival crevicular fluid (GCF) was analyzed by Western blotting and immunoradiometric assay (IRMA), respectively, in GCF of 6 healthy subjects just prior to and during orthodontics treatment using fixed appliances. RESULTS:We observed a significant time-dependent decrease of IGFBP-3 content in GCF during orthodontic treatment (4 h and 10 days). Reduction in levels of intact, glycosylated 47 kDa form of IGFBP-3 was associated with its degradation and the appearance of intermediate breakdown products. IGF-I levels were significantly increased 4 h after application of orthodontic force, while they were significantly reduced 10 days after the start of treatment. CONCLUSIONS:IGFBP-3 secretion into GCF and its molecular structure are modified by the fixed force of orthodontic treatment. Alterations in IGFBP-3 appear to be unrelated to the binding to IGF-I, suggesting an IGF-independent role of this binding protein in tooth movement.

journal_name

J Endocrinol Invest

authors

Toia M,Galazzo R,Maioli C,Granata R,Scarlatti F

doi

10.1007/BF03345340

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

1009-14

issue

11

eissn

0391-4097

issn

1720-8386

pii

2625

journal_volume

28

pub_type

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