Abstract:
Purpose:This research aimed to investigate the antibacterial activity and potential mechanism of luteolin against T. pyogenes. Materials and Methods:The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of luteolin against various T. pyogenes strains. The potential mechanism of action of luteolin was elucidated through testing and analysing the luteolin-induced alterations of T. pyogenes in several aspects, including cell wall, cell membrane, protein expression, nucleic acid content, topoisomerase activity and energy metabolism. Results:The MIC values of luteolin against various T. pyogenes isolates and ATCC19411 were 78 µg/mL. The increased cell membrane permeability, destruction of cell wall integrity and TEM images after exposure to luteolin showed that the cell wall and membrane were damaged. The content of total protein and nucleic acid in T. pyogenes decreased significantly after treatment with luteolin (1/2 MIC) for 12, 24, and 36 h. Moreover, a hypochromic effect was observed in the absorption spectrum of luteolin when deoxyribonucleic acid (DNA) was added. In addition, after treatment with luteolin, a decrease in nicked or relaxed DNA content, which was catalysed by T. pyogenes-isolated DNA topoisomerase, was observed. In addition, the adenosine triphosphate (ATP) content in cells and the activity of succinate dehydrogenase (SDH) both decreased when T. pyogenes was exposed to different concentrations (1/4 MIC, 1/2 MIC, 1 MIC, 2 MIC) of luteolin for 1 h. Conclusion:Luteolin showed distinct antibacterial activity against T. pyogenes by multiple actions, which mainly include destroying the integrity of the cell wall and cell membrane, influencing the expression of proteins, inhibiting nucleic acid synthesis, and interfering with energy metabolism.
journal_name
Infect Drug Resistjournal_title
Infection and drug resistanceauthors
Guo Y,Liu Y,Zhang Z,Chen M,Zhang D,Tian C,Liu M,Jiang Gdoi
10.2147/IDR.S253363subject
Has Abstractpub_date
2020-06-10 00:00:00pages
1697-1711issn
1178-6973pii
253363journal_volume
13pub_type
杂志文章abstract:Background:Broad-scale evidence has shown the significant association between ambient air pollutants and the development of tuberculosis (TB). However, the impact of air quality on the risk of TB in Taiwan is still poorly understood. Objective:To develop a probabilistic integrated population-level risk assessment appr...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S227823
更新日期:2019-12-06 00:00:00
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journal_title:Infection and drug resistance
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journal_title:Infection and drug resistance
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更新日期:2018-08-03 00:00:00
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更新日期:2014-08-19 00:00:00
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journal_title:Infection and drug resistance
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doi:10.2147/IDR.S263632
更新日期:2020-07-29 00:00:00
abstract:Purpose:To assess the efficacy of amoxicillin, tetracycline, high-dose metronidazole, and a proton-pump inhibitor for third-line Helicobacter pylori eradication. Methods:We enrolled 70 consecutive patients who had registered, failed to respond to two rounds of H. pylori eradication, and undergone endoscopy for H. pylo...
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doi:10.2147/IDR.S185511
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doi:10.2147/IDR.S228981
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journal_title:Infection and drug resistance
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doi:10.2147/IDR.S274589
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abstract::Raltegravir, the only integrase (IN) inhibitor approved for use in HIV therapy, has recently been licensed. Raltegravir inhibits HIV-1 replication by blocking the IN strand transfer reaction. More than 30 mutations have been associated with resistance to raltegravir and other IN strand transfer inhibitors (INSTIs). Th...
journal_title:Infection and drug resistance
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更新日期:2011-01-01 00:00:00
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journal_title:Infection and drug resistance
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更新日期:2018-03-12 00:00:00
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