Abstract:
Introduction:Understanding drug resistance is important in drug selection for Helicobacter pylori (H. pylori) eradication, and drug resistance data are lacking in Beijing. Purpose:This cross-sectional study aimed to isolate H. pylori from patients with gastroduodenal diseases and to analyze drug resistance to clarithromycin (CLA) and levofloxacin (LEV), which are used frequently in China. Patients and Methods:One hundred and seventy-six patients with gastroduodenal diseases undergoing gastroduodenoscopy were selected by convenient sampling. Gastric mucosa samples were cultured and sub-cultured using a new medium broth. Active H. pylori strains were confirmed by microscopy observation as Gram-negative curved bacilli with positive test results for urease, oxidase, and catalase, and H. pylori 16S rRNA amplification by polymerase chain reaction (PCR). CLA and LEV resistance was identified by minimum inhibitory concentration (MIC) tests and sequencing of 23S rRNA, gyrA, and gyrB genes. Results:From the 176 clinical samples, 112 (112/176, 63.6%) were confirmed with H. pylori infection and 65 (65/176, 36.9%) active H. pylori strains were obtained and further confirmed by MIC assay. Overall, the rates of CLA-resistant and LEV-resistant mutations in the 112 samples were 50.9% and 33.0%, respectively. Mutation related to CLA resistance was A2143G in the 23S rRNA gene and mutations associated with LEV resistance were N87K, D91G, and D91Y in the gyrA gene. Of 112 samples, 22 (19.6%) presented dual resistance to CLA and LEV. Resistance of the H. pylori strains to CLA (r=0.846, P<0.001) and LEV (r=0.936, P<0.001) had a strong correlation in phenotypic and genotypic level. Conclusion:The results indicated that resistance of CLA and LEV is severe among patients with gastroduodenitis. A good consistency could be found as to drug resistance between genotypic or phenotypic assay, suggested extending the detection of H. pylori drug resistance from the MIC method to a genotypic assay.
journal_name
Infect Drug Resistjournal_title
Infection and drug resistanceauthors
Lok CH,Zhu D,Wang J,Ren YT,Jiang X,Li SJ,Zhao XYdoi
10.2147/IDR.S249370subject
Has Abstractpub_date
2020-07-06 00:00:00pages
2145-2153issn
1178-6973pii
249370journal_volume
13pub_type
杂志文章abstract:INTRODUCTION:In recent decades, antimicrobial resistance has become a public health problem, particularly in cases of healthcare-associated infections. Interaction between antibiotic consumption and resistance development is of particular interest regarding Gram-negative bacilli, whose growing resistance has represente...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S22491
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abstract:Purpose:Antifungal resistance and virulence properties of Candida albicans (C. albicans) are growing health problems worldwide. The present study aims to investigate the effect of Zinc Oxide (ZnO) nanoparticles and Nystatin on SAP1-3 genes expression in C. albicans isolates of females with Vulvovaginal Candidiasis (VVC...
journal_title:Infection and drug resistance
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abstract:Objective:Evaluate host and pathogen factors associated with mortality in those with hospital acquired infections (HAI) in a tertiary intensive care unit in Brazil. Methods:Observational and analytical cohort single center study in a general intensive care unit (ICU) in Northeastern Brazil between January 2016 and Aug...
journal_title:Infection and drug resistance
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abstract:Objective:An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the determination of selinexor was established to investigate the effects of isavuconazole, itraconazole and fluconazole on the pharmacokinetics of selinexor in rats, respectively. Methods:Twenty-four healthy male rat...
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journal_title:Infection and drug resistance
pub_type: 杂志文章
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journal_title:Infection and drug resistance
pub_type: 杂志文章
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更新日期:2013-08-20 00:00:00
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journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
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更新日期:2013-09-18 00:00:00
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journal_title:Infection and drug resistance
pub_type: 杂志文章
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更新日期:2015-10-20 00:00:00
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journal_title:Infection and drug resistance
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更新日期:2018-01-09 00:00:00
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journal_title:Infection and drug resistance
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更新日期:2020-03-23 00:00:00
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journal_title:Infection and drug resistance
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doi:10.2147/IDR.S252332
更新日期:2020-06-04 00:00:00
abstract::Purpose: Treatment-refractory Giardia cases have increased rapidly within the last decade. No markers of resistance nor a standardized susceptibility test have been established yet, but several enzymes and their pathways have been associated with metronidazole (MTZ) resistant Giardia. Very limited data are available r...
journal_title:Infection and drug resistance
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doi:10.2147/IDR.S177997
更新日期:2019-05-10 00:00:00
abstract:Background:The incidence of human papilloma virus (HPV) infections in conservative populations is increasing, yet vaccination is not routinely recommended by primary health care (PHC) physicians. The aim of this study is to evaluate PHC physicians' awareness of HPV infections and vaccination in Saudi Arabia. Materials...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S179642
更新日期:2018-11-15 00:00:00
abstract::Raoultella ornithinolytica is an encapsulated Gram-negative, oxidase-negative, catalase-positive, aerobic, non-motile rod that belongs to the Enterobacteriaceae family. This bacterium was initially classified in the genus Klebsiella as Klebsiella ornithinolytica, until the creation of the genus Raoultella in 2001. R. ...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S191387
更新日期:2020-04-15 00:00:00
abstract::One of the main fundamental mechanisms of antibiotic resistance in Gram-negative bacteria comprises an effective change in the membrane permeability to antibiotics. The Gram-negative bacterial complex cell envelope comprises an outer membrane that delimits the periplasm from the exterior environment. The outer membran...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S144299
更新日期:2017-08-30 00:00:00
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journal_title:Infection and drug resistance
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更新日期:2020-05-07 00:00:00
abstract:Background and purpose:Invasive nontyphoidal salmonellosis, mostly caused by serovars Typhimurium and Enteritidis of Salmonella enterica, has emerged as a major public health problem in sub-Saharan Africa. The aim of this study was the clinical and microbiological characterization of nontyphoidal salmonellosis episodes...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S147243
更新日期:2018-01-31 00:00:00
abstract::Lucio's phenomenon (LP) is a special reactional state associated with diffuse multibacillary leprosy; both exhibit a limitative global distribution mainly in Mexico and Central America. We report a case of a 28-year-old female leprosy patient in the People's Republic of China, together with LP and positive anticardiol...
journal_title:Infection and drug resistance
pub_type:
doi:10.2147/IDR.S202386
更新日期:2019-04-02 00:00:00
abstract:Introduction:Klebsiella pneumoniae carbapenemase (KPC) belongs to the Group-A β-lactamases that incorporate serine at their active site and hydrolyze various penicillins, cephalosporins, and carbapenems. Metallo-beta-lactamases (MBLs) are group-B enzymes that contain one or two essential zinc ions in the active sites a...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S262493
更新日期:2020-08-18 00:00:00
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journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S175679
更新日期:2018-10-26 00:00:00
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journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S150839
更新日期:2018-02-13 00:00:00
abstract::Mycoplasma genitalium is one of the important causes of non-gonococcal urethritis. Rising incidence and emerging antimicrobial resistance are a major concern these days. The poor clinical outcomes with doxycycline therapy led to the use of azithromycin as the primary drug of choice. Single-dose azithromycin regimen ov...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S105469
更新日期:2017-09-01 00:00:00
abstract:Background:Leishmaniasis is an infectious disease common in tropical and subtropical regions caused by the genus Leishmania, which is transmitted by the bite of female sandflies. In this study, we evaluate the anti-leishmanial effect of recombinant Clostridium α-toxin protein alone and the combination with glucantime t...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S257561
更新日期:2020-07-15 00:00:00
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更新日期:2019-09-30 00:00:00
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journal_title:Infection and drug resistance
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更新日期:2020-10-15 00:00:00
abstract:Background:The aim of this study was to evaluate the clinical performance of the BD Kiestra InoqulA automated specimen processing system with commonly encountered clinical microbiology specimens. Materials and Methods:Four types of clinical specimens (sputum, urine, normally sterile body fluids, and feces) were inocul...
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更新日期:2020-04-01 00:00:00