Characterization of phenotypic and genotypic traits of carbapenem-resistant Acinetobacter baumannii clinical isolates recovered from a tertiary care hospital in Taif, Saudi Arabia.

Abstract:

Background and objective:Acinetobacter baumannii (A. baumannii) is a common nosocomial pathogen, which developed multi-drug-resistance to different classes of antibiotics including carbapenems. This study examined ten common carbapenemase genes among 32 carbapenem-resistant A. baumannii clinical isolates recovered from Taif, Saudi Arabia. Methods:Isolates were phenotypically identified to the genus level by Vitek®2 and API 20NE®. The species level was confirmed by the amplification of blaOXA-51. The susceptibility for 21 different antibiotics was performed by Vitek 2 and modified Kirby-Bauer method. Isolates were genetically screened for 10 carbapenemases. Phylogenetic relatedness between isolates was determined by ERIC-PCR. Results:Genotypically identified A. baumannii represented 100% of the total phenotypically identified Acinetobacter spp. All the carbapenem-resistant isolates were sensitive to polymyxin B and colistin. Among the other antibiotics, ampicillin/sulbactam and tigecycline were the most effective agents. 90.8% of the isolates were resistant to all ten investigated β-lactams. blaOXA-51,blaIPM, blaNDM and blaOXA-23 were detected in 100%, 87.5%, 62.5% and 59.4% of isolates, respectively. Also, blaVIM and blaOXA-40 were less prevalent and were detected in 9.3% and 3.1% of the isolates, respectively. In addition, blaKPC, blaOXA-48, blaOXA-58, blaOXA-181 were not detected in any isolate. The A. baumannii isolates were categorised into ten genotypes on the basis of the detected carbapenemase genes and ERIC-PCR revealed a remarkable clonal diversity among these isolates. Conclusion:Class A and class D carbapenemase genes were the most commonly detected among carbapenem resistant A. baumannii (CRAB) clinical isolates.

journal_name

Infect Drug Resist

authors

El-Badawy MF,Abdelwahab SF,Alghamdi SA,Shohayeb MM

doi

10.2147/IDR.S206691

subject

Has Abstract

pub_date

2019-10-03 00:00:00

pages

3113-3124

issn

1178-6973

pii

206691

journal_volume

12

pub_type

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