Effect of tetramethylpyrazine on potassium channels to lower calcium concentration in cultured aortic smooth muscle cells.

Abstract:

:1. Tetramethylpyrazine (TMP) is one of the active principles contained in Ligusticum chuanxiong Hort. (Umbelliferae), a herb that has been used widely in China to treat vascular disorders. 2. In an attempt to elucidate the possible mechanisms of action of TMP, the effect of TMP on intracellular calcium concentrations ([Ca2+]i) was investigated in cultured vascular smooth muscle (A7r5) cells using the Ca(2+)-sensitive dye Fura-2 as an indicator. 3. The increase in [Ca2+]i in A7r5 cells produced by vasopressin (1 micromol/L) or phenylephrine (1 micromol/L) was attenuated by TMP in a concentration-dependent manner. Only inhibitors specific to ATP-sensitive potassium (KATP) channels or small conductance calcium-activated potassium (SKCa) channels attenuated the action of TMP (10 micromol/L) on [Ca2+]i. However, blockers of other K+ channels failed to modify the inhibitory action of TMP (10 micromol/L) on [Ca2+]i. 4. The action of TMP on membrane potential in A7r5 cells was monitored by the fluorescence of bisoxonol. Tetramethylpyrazine caused a concentration-dependent inhibition of changes in membrane potential elicited by KCl (20 mmol/L) or phenylephrine (1 micro mol/L), an effect that was totally reversed by glibenclamide (100 micromol/L) and apamin (100 nmol/L) in combination. 5. The results obtained indicate that the decrease in [Ca2+]i in A7r5 cells produced by TMP is mediated mainly by opening of KATP and/or SKCa channels.

authors

Wong KL,Chan P,Huang WC,Yang TL,Liu IM,Lai TY,Tsai CC,Cheng JT

doi

10.1046/j.1440-1681.2003.03913.x

keywords:

subject

Has Abstract

pub_date

2003-10-01 00:00:00

pages

793-8

issue

10

eissn

0305-1870

issn

1440-1681

pii

3913

journal_volume

30

pub_type

杂志文章
  • Genetic factors regulate the rise in blood pressure in F2 generation crossed between stroke-prone spontaneously hypertensive rats and Wistar-Kyoto rats.

    abstract::1. There was no significant difference between the systolic blood pressure (SBP) of offspring derived from SHRSP mother and WKY father and the SBP of offspring derived from WKY mother and SHRSP father at the developing stage (5-13 weeks of age). 2. The degree of genetic determinations of SBP in stroke-prone spontaneou...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1991.tb01631.x

    authors: Ikeda K,Nara Y,Nabica T,Fujimoto A,Endo J,Ishino H,Yamori Y

    更新日期:1991-09-01 00:00:00

  • Noradrenergic reinnervation of the rat vas deferens after vasovasostomy following vasectomy.

    abstract::1. Anastomosis (vasovasostomy) of epididymal and prostatic halves of vasa deferentia was carried out in rats in which unilateral or bilateral medial transection (vasectomy) had been performed 4 weeks previously. 2. Noradrenaline levels in epididymal halves of vasa deferentia, 8 weeks after anastomosis with prostatic h...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1987.tb00984.x

    authors: DeGaris RM,Pennefather JN

    更新日期:1987-05-01 00:00:00

  • Hypertension through adrenaline activation of prejunctional beta-adrenoceptors.

    abstract::1. Adrenaline can enhance the stimulation-induced release of transmitter noradrenaline in sympathetically innervated tissues by activating prejunctional beta-adrenoceptors. 2. Adrenaline incorporated into sympathetic transmitter stores by neuronal uptake can be subsequently released as a co-transmitter and can then ac...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1981.tb00750.x

    authors: Majewski H,Tung LH,Rand MJ

    更新日期:1981-09-01 00:00:00

  • Involvement of both endoplasmic reticulum- and mitochondria-dependent pathways in cardiotoxin III-induced apoptosis in HL-60 cells.

    abstract::Cardiotoxin (CTX) III, a basic polypeptide with 60 amino acid residues isolated from Naja naja atra venom, has been reported to have anticancer activity. In the present study, we investigated the mechanisms underlying the anticancer activity of CTX III in human leukaemia (HL-60 cells). Cardiotoxin III activated the en...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2008.04968.x

    authors: Chien CM,Yang SH,Chang LS,Lin SR

    更新日期:2008-09-01 00:00:00

  • Pharmacokinetics and pharmacodynamics of a nitric oxide-releasing derivative of enalapril in male beagles.

    abstract::1. Pharmacological compounds that release nitric oxide (NO) have been useful tools in the evaluation of the broad role of NO in physiopathology and therapeutics. The present study compared the pharmacokinetics and pharmacodynamics of enalapril and an NO-releasing enalapril molecule (NCX899) in conscious male beagles. ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2007.04559.x

    authors: Okuyama CE,Mendes GD,Faro R,Rezende VM,Lagos RM,Astigarraga RE,Antunes E,De Nucci G

    更新日期:2007-04-01 00:00:00

  • Lithium attenuates d-amphetamine-induced hyperlocomotor activity in mice via inhibition of interaction between cyclooxygenase-2 and indoleamine-2,3-dioxygenase.

    abstract::In the present study, we investigated whether mood stabilizer lithium (Li) protects against d-amphetamine (AMP)-induced mania-like behaviours via modulating the novel proinflammatory potential. Repeated treatment with AMP resulted in significant increases in proinflammatory cyclooxygenase-2 (COX-2) and indolemaine-2,3...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/1440-1681.13243

    authors: Phan DH,Shin EJ,Jeong JH,Tran HQ,Sharma N,Nguyen BT,Jung TW,Nah SY,Saito K,Nabeshima T,Kim HC

    更新日期:2020-05-01 00:00:00

  • Effect of almitrine bismesylate on breathing pattern during hypoxia/hypercapnia in rats and ferrets.

    abstract::1. Ventilatory measurements and functional residual capacity (FRC) were recorded from anaesthetized rats and ferrets using a whole body plethysmograph. Simulation of aspects of human chronic obstructive airways disease (COAD) was attempted by making animals acutely hypoxic or hypoxic and hypercapnic by causing them to...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1987.tb02420.x

    authors: Austin CA,Wach RA,Bee D,Finlay M,Emery CJ,Suggett AJ,Barer GR

    更新日期:1987-11-01 00:00:00

  • Irinotecan (CPT-11): a brief overview.

    abstract::1. Irinotecan (also known as CPT-11) is a water soluble, semi-synthetic analogue of 20(S)camptothecin (CPT) with promising activity against a range of tumour types. 2. As with all other active analogues of CPT, irinotecan causes cell toxicity by stabilizing a ternary complex between the nuclear enzyme topoisomerase I ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.1996.tb01158.x

    authors: Rivory LP

    更新日期:1996-10-01 00:00:00

  • MiR-30a-5p ameliorates spinal cord injury-induced inflammatory responses and oxidative stress by targeting Neurod 1 through MAPK/ERK signalling.

    abstract::Spinal cord injury (SCI) is a major disability requiring more effective treatment than is currently available. MicroRNAs have been shown to effectively regulate gene expression at the translational level. The aim of the present study was to explore the potential role of miR-30-5p and possible mechanism in SCI. We foun...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/1440-1681.12856

    authors: Fu X,Shen Y,Wang W,Li X

    更新日期:2018-01-01 00:00:00

  • Competition by second messenger systems for receptor interaction and activation: implications for tissue-specific responses and disease therapy.

    abstract::1. At any one instant, most receptors are now recognized to be able to stimulate multiple signal transduction pathways in a cell when activated by an appropriate hormone. These different signalling pathways appear to allow for distinct cellular responses, such as cell proliferation, differentiation, and shape change. ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.1997.tb02104.x

    authors: Crouch MF,Frew IJ,Simson L,Davy DA

    更新日期:1997-08-01 00:00:00

  • Effects of anaesthesia on the removal from plasma of intravenously injected chylomicron-like lipid emulsions in rats and mice.

    abstract::1. In order to find an anaesthesia with minimum perturbation to the metabolism of chylomicrons, the effects of seven different anaesthetic agents on clearance from plasma of chylomicron-like emulsions were compared. 2. Avertin, urethane, fentanyl, and a ketamine/xylazine mixture all slowed the removal from plasma of e...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1993.tb01502.x

    authors: Mortimer BC,Umeda Y,Elsegood CL,Redgrave TG

    更新日期:1993-01-01 00:00:00

  • Effects of dopamine and nitric oxide on arterial pressure and renal function in volume expansion.

    abstract::1. The aim of the present study was to investigate the role of dopamine (DA) in the hypotensive and renal effects of L-arginine during extracellular fluid volume expansion (10% bodyweight). 2. Animals were randomized to non-expanded and expanded groups. Both groups received different treatments: L-arginine (250 mg/kg,...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1681.2002.03729.x

    authors: Costa Mde L,Loria A,Marchetti M,Balaszczuk AM,Arranz CT

    更新日期:2002-09-01 00:00:00

  • Histone modifications and skeletal muscle metabolic gene expression.

    abstract::1. Skeletal muscle oxidative function and metabolic gene expression are co-ordinately downregulated in metabolic diseases such as insulin resistance, obesity and Type 2 diabetes. Altering skeletal muscle metabolic gene expression to favour enhanced energy expenditure is considered a potential therapy to combat these d...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.2009.05311.x

    authors: McGee SL,Hargreaves M

    更新日期:2010-03-01 00:00:00

  • Dual nitric oxide mechanisms of cholestasis-induced bradycardia in the rat.

    abstract::1. Cholestatic liver disease is associated with nitric oxide (NO) overproduction and bradycardia. Nitric oxide has a dual effect on sinoatrial node and its effects depend on its concentration. Nitric oxide can increase heart rate by activating hyperpolarization-activated pacemaker current (If) but, at high concentrati...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1681.2002.03748.x

    authors: Mani AR,Nahavandi A,Moosavi M,Safarinejad R,Dehpour AR

    更新日期:2002-10-01 00:00:00

  • Opioid mechanisms controlling renal function.

    abstract::1. Over the past 50 years considerable evidence has been reported suggesting that endogenous opioids participate in the control of renal function. 2. Exogenous administration of opioids produces profound changes in the renal excretion of water and sodium. 3. Opioids produce changes in urine output and urine sodium exc...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.1995.tb02324.x

    authors: Kapusta DR

    更新日期:1995-12-01 00:00:00

  • Relationship between the dose-response effects of diazepam and clobazam on electroencephalographic parameters and on kindled amygdaloid seizure activity in rats.

    abstract::1. The possibility that the anticonvulsant activity of the benzodiazepines, diazepam and clobazam, is related to changes in EEG parameters, particularly beta activity, was investigated in amygdaloid kindled rats. 2. The effects of diazepam (1, 2, 4, 8 and 16 mumols/kg), administered intraperitoneally (i.p.), clobazam ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1988.tb01015.x

    authors: Harris QL,Lewis SJ,Young NA,Vajda FJ,Jarrott B

    更新日期:1988-10-01 00:00:00

  • Arterial calcification and stiffness in chronic kidney disease.

    abstract::1. Patients with chronic kidney disease (CKD) demonstrate a high burden of vascular disease. This vascular disease is unusual by way of a preponderance of medial calcification. Further, traditional cardiovascular risk factors fail to fully explain the high cardiovascular event rate in this population. 2. The present r...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1681.2007.04660.x

    authors: Kerr PG,Guerin AP

    更新日期:2007-07-01 00:00:00

  • Withdrawal of intravenous glyceryl trinitrate: absence of rebound phenomena with transition to oral isosorbide dinitrate.

    abstract::1. Glyceryl trinitrate (GTN) is frequently infused intravenously as a component of the management of acute coronary syndromes (ACS). Abrupt cessation of GTN infusion after periods of more than 24 h administration often induces rebound vasoconstriction reflecting 'pseudotolerance'; this is also the basis of the 'zero h...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1440-1681.2005.04182.x

    authors: Kelly EA,Ahmed RM,Horowitz JD

    更新日期:2005-04-01 00:00:00

  • Time course of changes in baroreceptor reflex control of heart rate in conscious SHR and WKY: contribution of the cardiac vagus and sympathetic nerves.

    abstract::1. The aim of this study was to assess the vagal and sympathetic nerve contribution to the relationship between mean arterial pressure (MAP) and heart rate (HR) at 6, 9, 14 and 20 weeks of age in conscious Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) with methoxamine- and nitroprusside-induced steady-s...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1988.tb01075.x

    authors: Head GA,Adams MA

    更新日期:1988-04-01 00:00:00

  • Brain versus peripheral angiotensin II receptors in hypovolaemia: behavioural and cardiovascular implications.

    abstract::1. Angiotensin (Ang)II is involved in responses to hypovolaemia, such as sodium appetite and increase in blood pressure. Target areas subserving these responses for AngII include the cardiovascular system in the periphery and the circumventricular organs in the brain. 2. Conflicting data have been reported for the rol...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1681.2000.03262.x

    authors: De Luca LA Jr,Sugawara AM,Menani JV

    更新日期:2000-05-01 00:00:00

  • Taurine prevents high-fat diet-induced-hepatic steatosis in rats by direct inhibition of hepatic sterol regulatory element-binding proteins and activation of AMPK.

    abstract::This study investigated if the protective effect of taurine against high fat diet-induced hepatic steatosis involves modulating the hepatic activity of 5' AMP-activated protein kinase (AMPK) and levels/activity of the sterol regulatory element-binding proteins-1/2 (SREBP1/2). Rats were divided into four groups (n = 12...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/1440-1681.13387

    authors: Morsy MD,Aboonq MS,ALsleem MA,Abusham AA

    更新日期:2020-07-21 00:00:00

  • Lack of effect of age on the cardiovascular response to neuropeptide Y injection in the rat nucleus tractus solitarius.

    abstract::1. Neuropeptide Y (NPY) is colocalized with catecholamines in central regions involved in blood pressure regulation and exerts depressor responses in the nucleus tractus solitarius (NTS). Ageing is accompanied by a decline in baroreflex function and a reduction in NPY concentrations in some brain areas. The present st...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1997.tb01801.x

    authors: Morris MJ,Pavia JM

    更新日期:1997-02-01 00:00:00

  • Chronic exercise partially restores the transmural heterogeneity of action potential duration in left ventricular myocytes of spontaneous hypertensive rats.

    abstract::Hypertension leads to electrophysiological changes in the heart. Chronic exercise induced by a treadmill-running programme (TRP) is considered a potential non-pharmacological treatment for hypertension and may have implications in heart remodelling. However, it is not known whether the TRP is able to improve the elect...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2011.05669.x

    authors: Roman-Campos D,Carneiro-Júnior MA,Prímola-Gomes TN,Silva KA,Quintão-Júnior JF,Gondim AN,Duarte HL,Cruz JS,Natali AJ

    更新日期:2012-02-01 00:00:00

  • Increased cardiac sympathetic drive and reduced vagal modulation following endothelin receptor antagonism in healthy conscious rats.

    abstract::1. The present study evaluated changes in autonomic control of the cardiovascular system in conscious rats following blockade of endothelin (ET) receptors with bosentan. 2. Rats were treated with bosentan or vehicle (5% gum arabic) for 7 days by gavage. 3. Baseline heart rate (HR) was higher in the bosentan-treated gr...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2008.04866.x

    authors: Souza HC,Terzini GC,da Silva VJ,Martins-Pinge MC,Salgado HC,Salgado MC

    更新日期:2008-07-01 00:00:00

  • RNA interference-mediated silencing of UBCH10 gene inhibits colorectal cancer cell growth in vitro and in vivo.

    abstract::1. UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme, and its overexpression has been demonstrated in a variety of malignancies. The aim of the present study is to silence UbcH10 gene by RNA interference (RNAi) and to observe its inhibitory effect on the colorectal cancer cell growth in vitro and in vivo. 2...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.2009.05348.x

    authors: Chen SM,Jiang CY,Wu JY,Liu B,Chen YJ,Hu CJ,Liu XX

    更新日期:2010-05-01 00:00:00

  • Streptokinase alters myocardial creatine kinase depletion after ischaemia and reperfusion in rabbits.

    abstract::The efficacy of streptokinase as an intracoronary thrombolytic agent is well-recognized. The effect of streptokinase, distinct from its thrombolytic action, on ischaemic myocardium distal to an area of coronary artery occlusion when reperfusion occurs has not been well-defined. In order to do this, myocardial creatine...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1986.tb02392.x

    authors: Mickelson JK,Carlson CJ,Margaretten W,Rapaport E

    更新日期:1986-09-01 00:00:00

  • Salt- and angiotensin II-dependent variations in amiloride-sensitive rectal potential difference in mice.

    abstract::1. In the rectum and distal nephron, sodium reabsorption is mediated by the amiloride-sensitive epithelial sodium channel (ENaC). The ENaC-mediated sodium transport is electrogenic and creates an amiloride-sensitive transepithelial potential difference (PD). 2. We have evaluated the salt- and angiotensin (Ang)II-depen...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1681.2000.03204.x

    authors: Wang Q,Horisberger JD,Maillard M,Brunner HR,Rossier BC,Burnier M

    更新日期:2000-01-01 00:00:00

  • The effects of 2-thiophenecarboxylic acid on serum ionic calcium and magnesium levels in rats.

    abstract::1. Administration of 2-thiophenecarboxylic acid to rats maintained on a low calcium diet resulted in a significant depression in the serum levels of total and ionized calcium and inorganic phosphate. 2. The serum magnesium levels were not altered by the drug. ...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1975.tb01828.x

    authors: Raman A,Chong YK

    更新日期:1975-03-01 00:00:00

  • Afferent control of vasopressin and renin release during haemorrhage in normal and autonomically blocked rabbits.

    abstract::1. The role of the arterial and cardiac baroreceptors on the arginine vasopressin (AVP) and plasma renin activity (PRA) responses to haemorrhage was studied in conscious rabbits. They were bled at a rate of approximately 3% of their blood volume (BV)/min, both when the autonomic nervous system (ANS) was intact and dur...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1992.tb00412.x

    authors: Courneya CA,Korner PI,Oliver JR,Woods RL

    更新日期:1992-11-01 00:00:00

  • Influence of variation in dietary sodium intake on biochemical indices of sympathetic activity in normal man.

    abstract::The effects of variation in dietary sodium intake on biochemical indices of sympathetic activity were investigated in normal subjects. Sodium restriction for 7 days (mean urinary sodium excretion 21 mmol/day, s.e.m. = 4) increased supine plasma noradrenaline concentration significantly (1.78 nmol/l, s.e.m. = 0.23) com...

    journal_title:Clinical and experimental pharmacology & physiology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1681.1984.tb00253.x

    authors: Watson RD,Esler MD,Leonard P,Korner PI

    更新日期:1984-03-01 00:00:00