Detection of Listeria monocytogenes in a patient with meningoencephalitis using next-generation sequencing: a case report.

Abstract:

BACKGROUND:Listeria monocytogenes (L. monocytogenes) is a facultative intracellular bacterial pathogen which can invade different mammalian cells and reach to the central nervous system (CNS), leading to meningoencephalitis and brain abscesses. In the diagnosis of L. monocytogenes meningoencephalitis (LMM), the traditional test often reports negative owing to the antibiotic treatment or a low number of bacteria in the cerebrospinal fluid. To date, timely diagnosis and accurate treatment remains a challenge for patients with listeria infections. CASE PRESENTATION:We present the case of a 66-year-old woman whose clinical manifestations were suspected as tuberculous meningoencephalitis, but the case was finally properly diagnosed as LMM by next-generation sequencing (NGS). The patient was successfully treated using a combined antibacterial therapy, comprising ampicillin and trimethoprim-sulfamethoxazole. CONCLUSION:To improve the sensitivity of LMM diagnosis, we used NGS for the detection of L. monocytogenes. Hence, the clinical utility of this approach can be very helpful since it provides quickly and trust results.

journal_name

BMC Infect Dis

journal_title

BMC infectious diseases

authors

Lan ZW,Xiao MJ,Guan YL,Zhan YJ,Tang XQ

doi

10.1186/s12879-020-05447-z

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

721

issue

1

issn

1471-2334

pii

10.1186/s12879-020-05447-z

journal_volume

20

pub_type

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