Abstract:
BACKGROUND:Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a specific lymphocyte transformation assay to Cryptococcus neoformans in order to identify immunodeficiency associated to neurocryptococcosis (NCC) as primary cause of the mycosis. METHODS:Healthy volunteers, poultry growers, and HIV-seronegative patients with neurocryptococcosis were tested for cellular immune response. Cryptococcal meningitis was diagnosed by India ink staining of cerebrospinal fluid and cryptococcal antigen test (Immunomycol-Inc, SP, Brazil). Isolated peripheral blood mononuclear cells were stimulated with C. neoformans antigen, C. albicans antigen, and pokeweed mitogen. The amount of 3H-thymidine incorporated was assessed, and the results were expressed as stimulation index (SI) and log SI, sensitivity, specificity, and cut-off value (receiver operating characteristics curve). We applied unpaired Student t tests to compare data and considered significant differences for p<0.05. RESULTS:The lymphotoxin alpha showed a low capacity with all the stimuli for classifying patients as responders and non-responders. Lymphotoxin alpha stimulated by heated-killed antigen from patients with neurocryptococcosis was not affected by TCD4+ cell count, and the intensity of response did not correlate with the clinical evolution of neurocryptococcosis. CONCLUSION:Response to lymphocyte transformation assay should be analyzed based on a normal range and using more than one stimulator. The use of a cut-off value to classify patients with neurocryptococcosis is inadequate. Statistical analysis should be based on the log transformation of SI. A more purified antigen for evaluating specific response to C. neoformans is needed.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Rocha KC,Pinhal C,Cavalcanti S,Vidal MS,Toscano M,Moraes-Vasconcelos D,Duarte AJ,Fonseca FL,de Abreu LC,Valenti VE,Grumach ASdoi
10.1186/1471-2334-12-278subject
Has Abstractpub_date
2012-10-30 00:00:00pages
278issn
1471-2334pii
1471-2334-12-278journal_volume
12pub_type
杂志文章abstract:BACKGROUND:The real burden of smear-positive (PTB+) and bacteriologically confirmed tuberculosis (BCTB) in Ethiopia is not known. Thus, the aim of this community-based study was to measure the prevalence and incidence of tuberculosis in the Hetosa District of Oromia Region, Ethiopia. METHODS:First, a population-based ...
journal_title:BMC infectious diseases
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更新日期:2017-03-16 00:00:00
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pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2014-02-01 00:00:00
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journal_title:BMC infectious diseases
pub_type: 杂志文章
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更新日期:2019-12-31 00:00:00
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pub_type: 杂志文章
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更新日期:2016-08-26 00:00:00
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更新日期:2013-08-23 00:00:00
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更新日期:2007-08-16 00:00:00