Abstract：
:The response of Meishan conceptuses to an exogenous precursor for oestradiol-17beta biosynthesis was investigated in vitro, to determine whether gestational age or morphological stage of development elicit changes in hormone metabolism. Conceptuses were recovered on days 11, 12, 13 or 15 after the onset of oestrus and cultured for 6 hours at 37 degrees C, in the presence or absence of testosterone. On days 12 and 13 after the onset of oestrus spherical conceptuses were recovered from some gilts, whereas others yielded elongated or filamentous conceptuses. All conceptuses recovered on day 15 after oestrus had elongated. The number of cells per individual conceptus increased from days 11 to 13 after the onset of oestrus (P < 0.001), as did conceptus surface area (P = 0.038). Supplementing culture media with testosterone, as a substrate for oestrogen biosynthesis, significantly increased conceptus oestradiol-17beta secretion in vitro on days 12, 13 and 15, regardless of whether pre- or post-elongation conceptuses were cultured. However, on day 11 oestradiol-17beta was only detected at significant concentrations in the culture media of four testosterone supplemented conceptuses and only one gilt produced conceptuses capable of secreting oestradiol-17beta in the absence of testosterone. Therefore, the onset of conceptus oestradiol-17beta secretion is apparently limited by the expression of aromatase enzymes that are activated synchronously, irrespective of the stage of morphological development, within Meishan litters. Once established, Meishan conceptus oestradiol-17beta secretion in vitro is increased in the presence of exogenous testosterone.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Pickard AR,Miller SJ,Ashworth CJdoi
10.1186/1477-7827-1-16keywords:
subject
Has Abstractpub_date
2003-02-11 00:00:00pages
16issn
1477-7827journal_volume
1pub_type
杂志文章abstract：BACKGROUND:The study was designed to test the hypothesis that granulosa cell (GC) gene expression response differs between recombinant FSH and human menopausal gonadotropin (hMG) stimulation regimens. METHODS:Females < 35 years-old undergoing IVF for tubal or male factor infertility were prospectively randomized to on...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-8-25
更新日期：2010-03-12 00:00:00
abstract：:Fertilin alpha (ADAM-1) and beta (ADAM-2) are integral membrane proteins of the ADAM family that form a fertilin complex involved in key steps of the sperm-oocyte membrane interaction. In the present work, we analyzed the presence of ADAM-1 and ADAM-2 mRNAs, the spermatozoa proteins' processing and their sub-cellular ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-9-96
更新日期：2011-06-30 00:00:00
abstract：BACKGROUND:The aim of this study was to determine the relationship between a purported luteinizing hormone/chorionic gonadotropin (LHCGR) high function polymorphism (rs4539842/insLQ) and outcome to controlled ovarian hyperstimulation (COH). METHODS:This was a prospective study of 172 patients undergoing COH at the Fer...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-71
更新日期：2013-07-25 00:00:00
abstract：BACKGROUND:Macrophage migration inhibitory factor (MIF) has special pro-inflammatory roles, affecting the functions of macrophages and lymphocytes and counter-regulating the effects of glucocorticoids on the immune response. The conspicuous expression of MIF during human implantation and early embryonic development als...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-95
更新日期：2010-08-04 00:00:00
abstract：BACKGROUND:To evaluate the relationship between endometrial thickness on day of human chorionic gonadotrophin administration (hCG) and pregnancy outcome in a large number of consecutive in vitro fertilization and embryo transfer (IVF-ET) cycles. METHODS:A retrospective cohort study including all patients who had IVF-E...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-6-37
更新日期：2008-09-02 00:00:00
abstract：BACKGROUND:Premutation range CGGn repeats of the FMR1 gene denote risk toward primary ovarian insufficiency (POI), also called premature ovarian failure (POF). This prospective cohort study was undertaken to determine if X-chromosome inactivation skew (sXCI) is associated with variations in FMR1 CGG repeat length and, ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-017-0250-9
更新日期：2017-04-28 00:00:00
abstract：BACKGROUND:Intracytoplasmic morphologically selected sperm injection (IMSI) is still proposed and employed in the clinical practice to improve the reproductive outcome in infertile couples scheduled for conventional intracytoplasmic sperm injection (cICSI). The aim of the current randomized controlled trial (RCT) was t...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验,收录出版
doi：10.1186/s12958-015-0096-y
更新日期：2015-08-27 00:00:00
abstract：BACKGROUND:We demonstrated that E2 conjugated to BSA (E2BSA) induces a rapid membrane-initiated inhibition of LH secretion followed hours later by a slight increase in LH secretion. Whether these actions of E2BSA are restricted to the pituitary gland and whether the membrane-initiated pathway of E2BSA contributes to th...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-8-40
更新日期：2010-05-10 00:00:00
abstract：:The corpus luteum is an endocrine gland whose limited lifespan is hormonally programmed. This debate article summarizes findings of our research group that challenge the principle that the end of function of the corpus luteum or luteal regression, once triggered, cannot be reversed. Overturning luteal regression by ph...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-4-53
更新日期：2006-10-30 00:00:00
abstract：BACKGROUND:Matrix metalloproteinase 2 (MMP-2) has been reported to be an important regulator of cell migration and invasion through degradation of the extracellular matrix (ECM) in many diseases, such as cancer and endometriosis. Here, we found calcium-activated neutral protease 7 (CAPN 7) expression was markedly upreg...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-64
更新日期：2013-07-15 00:00:00
abstract：BACKGROUND:Spermatozoa are extremely vulnerable to oxidative stress caused by the unbalance between concentrations of reactive oxygen species and antioxidant scavenging systems present inside the male reproductive tract. In spite of a large number of clinical studies that claimed the beneficial effects of antioxidant o...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-81
更新日期：2013-08-16 00:00:00
abstract：BACKGROUND:We found previously that the expression of SET gene was up-regulated in polycystic ovaries. Evidences suggested that SET protein was essential for regulating both the promoter activity of CYP17A1 and the biological activity of P450c17. In this study, we explored whether SET regulated androgen production in p...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-9
更新日期：2013-02-19 00:00:00
abstract：BACKGROUND:Leukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to det...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-128
更新日期：2009-11-19 00:00:00
abstract：BACKGROUND:In animals, low levels of vitamin D are associated with estrus cycle disturbances, but there are virtually no human data. We examined the association of 25-hydroxyvitamin D (25(OH)D) (a biomarker for vitamin D status) with menstrual cycle characteristics. METHODS:Women aged 35-44 were randomly selected from...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0012-5
更新日期：2015-03-11 00:00:00
abstract：BACKGROUND:In young women with poor ovarian response, luteal-phase ovarian stimulation (LPOS) is a potential method for collecting competent oocytes. The aim of this study was to assess the efficacy of LPOS compared with follicular phase ovarian stimulation (FPOS) in young women with poor ovarian response (POR). METHO...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12958-020-00570-7
更新日期：2020-02-07 00:00:00
abstract：BACKGROUND:Sperm of poor quality can negatively affect embryo development to the blastocyst stage. The aim of this comparative prospective randomized study was to evaluate the role of an intracytoplasmic morphologically selected sperm injection (IMSI) in the same infertile couples included in the programme of intracyto...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-9-123
更新日期：2011-08-29 00:00:00
abstract：BACKGROUND:Doxorubicin is a potent chemotherapeutic drug used against a variety of cancers. It acts through interaction with polymerases and topoisomerase II and free radical production. Doxorubicin activity is not specific to cancer cells and can also damage healthy cells, especially those undergoing rapid proliferati...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-79
更新日期：2012-09-11 00:00:00
abstract：BACKGROUND:Embryo selection has been an integral feature of in vitro fertilization (IVF) almost since its inception. Since the advent of extended blastocyst stage embryo culture, and especially with increasing popularity of elective single embryo transfer (eSET), the concept of embryo selection has increasingly become ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-13-3
更新日期：2015-01-11 00:00:00
abstract：BACKGROUND:Methadone is the therapeutic agent of choice for the treatment of opiate addiction in pregnancy. The co-consumption (heroin, cocaine) which may influence the effects of methadone is frequent. Therefore, the impact of cocaine and heroin on the placental transfer of methadone and the placental tissue was inves...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-61
更新日期：2009-06-11 00:00:00
abstract：BACKGROUND:Morphological aspect of polycystic ovaries (PCO) is a very common finding in an IVF center population: this includes PCOS patients identified in 18-25% of the couples presenting with infertility and so called "sonographic PCO only" the prevalence of which has been estimated as high as 33% in asymptomatic pat...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 临床试验,杂志文章
doi：10.1186/1477-7827-11-54
更新日期：2013-06-19 00:00:00
abstract：:During the follicular phase of the primate menstrual cycle, a single follicle usually matures to the preovulatory stage and releases its oocyte for fertilization and the potential establishment of pregnancy. In assisted reproductive technology procedures, it is desirable to override the natural process of follicle sel...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-2-31
更新日期：2004-06-16 00:00:00
abstract：BACKGROUND:Coordinated differentiation of the endometrial compartments in the second half of the menstrual cycle is a prerequisite for the establishment of pregnancy. Endometrial stromal cells (ESC) decidualize under the influence of ovarian progesterone to accommodate implantation of the blastocyst and support establi...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-76
更新日期：2009-07-20 00:00:00
abstract：BACKGROUND:ATF6α, one of the sensor proteins in the stress signaling pathway of the endoplasmic reticulum, is located in the membrane of the endoplasmic reticulum. To date, the physiological function of ATF6α in the process of embryo implantation has not been reported. METHODS:In this study, the expression pattern of ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-016-0199-0
更新日期：2016-10-07 00:00:00
abstract：:Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 9-18% of women in reproductive age that causes hyperandrogenism and infertility due to dysfunctional follicular maturation and anovulation. The etiology of PCOS is still poorly known, and information from experimental animal models may help imp...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/s12958-017-0231-z
更新日期：2017-02-10 00:00:00
abstract：BACKGROUND:Mares have an annual reproductive rhythm, with a phase of inactivity in midwinter. The aim of this study was to determine the impact of food restriction on physiological and metabolic hallmarks of this rhythm. METHODS:Over three successive years, 3 groups of 10 mares were kept under natural photoperiod. A '...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-9-130
更新日期：2011-09-29 00:00:00
abstract：BACKGROUND:The association of recombinant FSH plus recombinant LH in 2:1 ratio may be used not only to induce ovulation in anovulatory women with hypogonadotropic hypogonadism but also to achieve multiple follicular developments in human IVF. The aim of this analysis was to estimate the cost-effectiveness of Controlled...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0386-2
更新日期：2018-07-18 00:00:00
abstract：BACKGROUND:Endometriosis is a well-known cause of infertility, and the anti-Mullerian hormone (AMH) is an accepted biomarker of ovarian reserve and response to artificial reproductive technology procedures. The present study was a prospective analysis of age-dependent AMH serum concentration in women with bilateral and...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0125-x
更新日期：2015-11-24 00:00:00
abstract：BACKGROUND:Overweight and obese women have been shown to have an increased risk of recurrent miscarriage as well as other adverse reproductive outcomes, but it is yet unclear whether this is due to an effect on the endometrium, embryo or both. The current study employs proteomic analysis to examine for a potential endo...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-12-75
更新日期：2014-08-05 00:00:00
abstract：BACKGROUND:Considering that clinical trial studies are limited in polycystic ovary syndrome (PCOS) patients, and there is no consensus on an optimum endometrial preparation protocol for frozen embryo transfer (FET), the present study was designed as a randomized clinical trial to compare the reproductive outcomes follo...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-00675-z
更新日期：2020-11-20 00:00:00
abstract：BACKGROUND:Endocannabinoids (ECs) are important contributors to implantation and decidualization and are suppressed in early pregnancy. Elevated levels of anandamide (AEA), the endogenous ligand for the CB1 and CB2 receptors (R), interfere with receptivity of the blastocyst. Ang-(1-7) is down-regulated in the implantat...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-13-5
更新日期：2015-01-17 00:00:00
