Abstract：
BACKGROUND:Overweight and obese women have been shown to have an increased risk of recurrent miscarriage as well as other adverse reproductive outcomes, but it is yet unclear whether this is due to an effect on the endometrium, embryo or both. The current study employs proteomic analysis to examine for a potential endometrial defect in obese and overweight women with recurrent miscarriage. METHODS:Proteomic tissue analysis of 21 endometrial samples obtained In the midluteal phase from 16 women with recurrent miscarriage (obese, n=12 and lean, n=4) and 5 fertile volunteers (Obese, n=2 and Lean, n=3). Proteins were separated using 2-D gel electrophoresis and principle component analysis was used to quantitatively compare protein expression between groups. Protein spots showing significantly altered expression were identified using mass spectrometry. RESULTS:Obese and overweight recurrent miscarriage patients had a significantly increased endometrial expression of haptoglobin compared to their lean counterparts (p=0.01). These patients also displayed a significant increase in endometrial expression of transthyretin (p=0.04) and beta- globulin (p=0.04). Principle Component Analysis (PCA) of the studied groups also demonstrated that endometrial samples could be grouped based on differences in the BMI, suggesting that obesity is an independent factor influencing endometrial protein expression. CONCLUSIONS:These findings provide preliminary evidence for an alteration in the endometrial protein profile in overweight/obese women with recurrent miscarriage mainly in the form of increased haptoglobin, an inflammatory marker associated with obesity.
journal_name
Reprod Biol Endocrinoljournal_title
Reproductive biology and endocrinology : RB&Eauthors
Metwally M,Preece R,Thomas J,Ledger W,Li TCdoi
10.1186/1477-7827-12-75subject
Has Abstractpub_date
2014-08-05 00:00:00pages
75issn
1477-7827pii
1477-7827-12-75journal_volume
12pub_type
杂志文章abstract：:Endometrial carcinoma is the most common neoplasm of the female genital tract, accounting for nearly one half of all gynecologic cancers in the Western world. Although intensive research on pathological phenomena of endometrial cancer is currently going on, but exact cause and biological aspects of this disease are no...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-81
更新日期：2009-08-05 00:00:00
abstract：BACKGROUND:The objective of this study was to identify substantially increased proteins in bovine cystic follicular fluid (FF) in order to clarify the pathology and etiology of bovine ovarian follicular cysts (BOFC). METHODS:Proteins in normal and cystic FF samples were subjected to two-dimensional polyacrylamide gel ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-3-23
更新日期：2005-06-08 00:00:00
abstract：BACKGROUND:This study was aimed at screening out the potential key genes and pathways associated with gestational diabetes mellitus (GDM). METHODS:The GSE70493 dataset used for this study was obtained from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) in the placental tissue of women with...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-019-0546-z
更新日期：2019-11-29 00:00:00
abstract：BACKGROUND:The alterations of the glutathione peroxidase enzyme complex system occur in physiological conditions such as aging and oxidative stress consequent to strenuous exercise. METHODS:Authors optimize the spectrophotometric method to measure glutathione peroxidase activity in rat red blood cell membranes. RESUL...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-7-7
更新日期：2009-01-26 00:00:00
abstract：BACKGROUND:In-situ hybridisation studies demonstrate that Notch receptors and ligands are expressed in granulosa cells (GCs) and in the theca layer vasculature of growing follicles. Notch signaling involves cell-to-cell interaction mediated by transmembrane receptors and ligands. This signaling pathway may represent a ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-43
更新日期：2013-05-15 00:00:00
abstract：:Human spermatozoa generate low levels of reactive oxygen species in order to stimulate key events, such as tyrosine phosphorylation, associated with sperm capacitation. However, if the generation of these potentially pernicious oxygen metabolites becomes elevated for any reason, spermatozoa possess a limited capacity ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
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更新日期：2005-11-29 00:00:00
abstract：BACKGROUND:The syncytialization of cytotrophoblast cells to syncytiotrophoblast is central to human placental transport and hormone production. Many techniques for in vitro study of this process have been proposed and new investigators to the field may find the literature in the field daunting. Here, we present a strai...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-015-0070-8
更新日期：2015-07-09 00:00:00
abstract：BACKGROUND:In young women with poor ovarian response, luteal-phase ovarian stimulation (LPOS) is a potential method for collecting competent oocytes. The aim of this study was to assess the efficacy of LPOS compared with follicular phase ovarian stimulation (FPOS) in young women with poor ovarian response (POR). METHO...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12958-020-00570-7
更新日期：2020-02-07 00:00:00
abstract：BACKGROUND:Brain-derived ectonucleoside triphosphate diphosphohydrolases (NTPDases) have been known as plasma membrane-incorporated enzymes with their ATP-hydrolyzing domain outside of the cell. As such, these enzymes are thought to regulate purinergic intercellular signaling by hydrolyzing ATP to ADP-AMP, thus regulat...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
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更新日期：2009-06-16 00:00:00
abstract：BACKGROUND:In early pregnancy, increased plasma levels of the endocannabinoid anandamide (AEA) are associated with miscarriage through mechanisms that might affect the developing placenta or maternal decidua. METHODS:In this study, we compare AEA levels in failed and viable pregnancies with the levels of the trophobla...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-9-152
更新日期：2011-11-29 00:00:00
abstract：PURPOSE:To investigate the associations between blastocyst development and the sex ratio (male:female) among singleton live births resulting from single-blastocyst frozen embryo transfer (FET) cycles. METHODS:Patients with singleton live births following the first autologous single FET of non- preimplantation genetic ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-00623-x
更新日期：2020-07-15 00:00:00
abstract：BACKGROUND:The association of recombinant FSH plus recombinant LH in 2:1 ratio may be used not only to induce ovulation in anovulatory women with hypogonadotropic hypogonadism but also to achieve multiple follicular developments in human IVF. The aim of this analysis was to estimate the cost-effectiveness of Controlled...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0386-2
更新日期：2018-07-18 00:00:00
abstract：:During rat estrous cycle, the endometrium proliferates in response to sex steroids and specific endometrial epithelial cells undergo apoptosis in absence of embryonic factors. The central executioner of apoptosis is a family of aspartic acid-specific cysteine proteases known as caspases. Smac/DIABLO is released from t...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-1-59
更新日期：2003-08-27 00:00:00
abstract：:Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced. High abortion and fetal mortality rates are commonly observed. These developmental defects have been attributed to incomple...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/1477-7827-1-98
更新日期：2003-11-13 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0376-4
更新日期：2018-06-12 00:00:00
abstract：:Although much is known about the reproductive biology of pond-breeding frogs, there is comparatively little information about terrestrial-breeding anurans, a highly successful and diverse group. This study investigates the activation and in vitro fertilization of eggs of the Puerto Rican coqui frog obtained by hormona...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-2-60
更新日期：2004-08-05 00:00:00
abstract：BACKGROUND:The aim of this randomized controlled trial was to compare the efficacy of extended letrozole regimen with clomiphene citrate in women with unexplained infertility undergoing superovulation and intrauterine insemination (IUI). METHODS:Two hundred and fourteen patients with unexplained infertility were rando...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2011-06-21 00:00:00
abstract：BACKGROUND:Spermatic cord torsion can lead to testis ischemia (I) and subsequent ischemia-reperfusion (I/R) causing germ cell-specific apoptosis. Previously, we demonstrated that the hypoxia-inducible factor-1 (HIF-1) transcription factor, a key regulator of physiological responses to hypoxia, is abundant in Leydig cel...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-104
更新日期：2012-12-05 00:00:00
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journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
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更新日期：2016-09-02 00:00:00
abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) has been recognized as a metabolic disorder, manifested by abdominal obesity, insulin resistance, dyslipidemia and hypertension. Pigment epithelium-derived factor (PEDF), a member of the serine protease inhibitor family, is a pleiotropic protein known for its antiangiogenic, ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-77
更新日期：2013-08-14 00:00:00
abstract：BACKGROUND:Infertile men have higher levels of semen reactive oxygen species (ROS) than fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This study investigated the effects of supplementation with N-acetyl-cysteine (NAC) on the sperm quality, c...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/s12958-019-0468-9
更新日期：2019-02-16 00:00:00
abstract：BACKGROUND:To evaluate the relationship between endometrial thickness on day of human chorionic gonadotrophin administration (hCG) and pregnancy outcome in a large number of consecutive in vitro fertilization and embryo transfer (IVF-ET) cycles. METHODS:A retrospective cohort study including all patients who had IVF-E...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-6-37
更新日期：2008-09-02 00:00:00
abstract：BACKGROUND:L-carnitine (LC), and its acetylated form, acetyl L-carnitine (ALC), have immense functional capabilities to regulate the oxidative and metabolic status of the female reproductive system. The vulnerability of this system to free radicals demand for advanced strategies to combat them. For this purpose, the 'q...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,评审
doi：10.1186/s12958-018-0323-4
更新日期：2018-01-26 00:00:00
abstract：BACKGROUND:Experiments were designed to identify the developmental expression and function of the Dickkopf-Like1 (DKKL1/Dkkl1) gene in humans and mice. METHODS:Mouse testes cDNA samples were collected at multiple postnatal times (days 4, 9, 18, 35, and 54, as well as at 6 months) and hybridized to Affymetrix mouse who...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-10-51
更新日期：2012-07-21 00:00:00
abstract：BACKGROUND:Hatching is crucial for mammalian embryo implantation, since difficulties during this process can lead to implantation failure, ectopic pregnancy and consequent infertility. Despite years of intensive researches, how internal and external factors affecting embryo hatch are still largely unclear. METHODS:The...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-018-0356-8
更新日期：2018-04-21 00:00:00
abstract：BACKGROUND:The aim of this study was to explore the impact of endometrial thickness change after progesterone administration on pregnancy outcome in patients transferred with single frozen-thawed blastocyst. METHODS:This observational cohort study included a total of 3091 patients undergoing their first frozen-thawed ...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-019-0545-0
更新日期：2019-11-25 00:00:00
abstract：BACKGROUND:The E2F family of transcription factors is required for the activation or repression of differentially expressed gene programs during the cell cycle in normal and abnormal development of tissues. We previously determined that members of the retinoblastoma protein family that interacts with the E2F family are...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-4-63
更新日期：2006-12-05 00:00:00
abstract：BACKGROUND:Considering that clinical trial studies are limited in polycystic ovary syndrome (PCOS) patients, and there is no consensus on an optimum endometrial preparation protocol for frozen embryo transfer (FET), the present study was designed as a randomized clinical trial to compare the reproductive outcomes follo...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/s12958-020-00675-z
更新日期：2020-11-20 00:00:00
abstract：BACKGROUND:The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000°C/min). Little published data is currently available on this device. This study presents our initial clinica...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章
doi：10.1186/1477-7827-11-41
更新日期：2013-05-15 00:00:00
abstract：BACKGROUND:This study was designed to determine whether small diameter needles for oocyte retrieval alter oocyte yields in patients undergoing IVF in comparison to standard large diameter needles. METHODS:We conducted a prospective pilot study of 21 consecutive favorable prognosis patients. In each patient one ovary w...
journal_title：Reproductive biology and endocrinology : RB&E
pub_type： 杂志文章,随机对照试验
doi：10.1186/1477-7827-11-22
更新日期：2013-03-20 00:00:00
